Browse > Article
http://dx.doi.org/10.11110/kjpt.2022.52.2.102

A study of the chromosome number and genome size of the rare species Rhododendron keiskei var. hypoglaucum in Korea  

CHOI, Bokyung (Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University)
KIM, Hyeonjin (Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University)
BYUN, Hye-Joo (Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University)
GANG, Geun-Hye (Plant Conservation Center, Korea National Park Research Institute)
LEE, Yongsoon (Department of Biology Education, Kongju National University)
MYEONG, Hyeon-Ho (Korea National Park Research Institute)
SO, Soonku (Plant Conservation Center, Korea National Park Research Institute)
JANG, Tae-Soo (Department of Biological Science, College of Bioscience and Biotechnology, Chungnam National University)
Publication Information
Korean Journal of Plant Taxonomy / v.52, no.2, 2022 , pp. 102-107 More about this Journal
Abstract
Rhododendron keiskei var. hypoglaucum (Ericaceae) was recently reported in Korea, with a disjunct distribution on the southern islands of the Korean Peninsula. Although chromosome numbers and ploidy variations are important traits in angiosperms, gaining a clear understanding the cytological features of Rhododendron has been hampered by the small size of its chromosomes. We herein report the chromosome number, karyotype structure, and genome size of R. keiskei var. hypoglaucum for the first time. The chromosome number of the investigated plants was 2n = 26 with x = 13 as the base chromosome number, which is the one of the frequently encountered base chromosome numbers in Rhododendron. The karyotype of R. keiskei var. hypoglaucum is composed of metacentric and submetacentric chromosomes similar in length, which ranged from 1.39 to 2.40 ㎛. The DNA 1C-value in all examined accessions was small, ranging from 0.63 to 0.65 pg, further supporting the stable genome size in Rhododendron. These comprehensive cytological results provide a framework for detailed molecular, cytogenetic, and phylogenomic analyses that can be used to interpret the slow species diversification rate in Rhododendron.
Keywords
Chromosome number; flow cytometry; genome size; karyotype; Rhododendron keiskei var. hypoglaucum;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Choi, B., S. Yang, J.-H. Song and T.-S. Jang. 2019. Karyotype and genome size variation in Ajuga L. (Ajugoideae-Lamiaceae). Nordic Journal of Botany 37: e02337.
2 Pessoa E. M., F. Nollet, R. F. Magalhaes, J. Viruel, F. Pinheiro and M. W. Chase. 2022. Nuclear-plastid discordance indicates past introgression in Epidendrum species (Laeliinae: Orchidaceae) with highly variable chromosome numbers. Botanical Journal of the Linnean Society 199: 357-371.   DOI
3 Redpath, L. E., R. Aryal, N. Lynch, J. A. Spencer, A. M. HulseKemp, J. R. Ballington, J. Green, N. Bassil, K. Hummer, T. Ranney and H. Ashrafi. 2022. Nuclear DNA contents and ploidy levels of North American Vaccinium species and interspecific hybrids. Scientia Horticulturae 297: 110955.   DOI
4 Stebbins, G. L. Jr. 1938. Cytological characteristics associated with the different growth habits in the dicotyledons. American Journal of Botany 25: 189-198.   DOI
5 Ammal, E. K. 1950. Polyploidy in the genus Rhododendron. The Rhododenron Year Book 5: 92-96.
6 Agudo, A. B., R. Torices, J. Loureiro, S. Castro, M. Castro and I. Alvarez. 2019. Genome size variation in a hybridizing diploid species complex in Anacyclus (Asteraceae: Anthemideae). International Journal of Plant Sciences 180: 374-385.   DOI
7 Atkinson, R., K. Jong and G. Argent. 2000. Chromosome numbers of some tropical Rhododendrons (section Vireya). Edinburgh Journal of Botany 57: 1-7.   DOI
8 Lysak, M. A., A. Berr, A. Pecinka, R. Schmidt, K. McBreen and I. Schubert. 2006. Mechanisms of chromosome number reduction in Arabidopsis thaliana and related Brassicaceae species. Proceedings of the National Academy of Sciences of the United States of America 103: 5224-5229.   DOI
9 Greimler, J., E. M. Temsch, Z. Xue, H. Weiss-Schneeweiss, P. Volkova, M. Peintinger, P. Wasowicz, H. Shang, I. Schanzer and J. O. Chiapella. 2022. Genome size variation in Deschampsia cespitosa sensu lato (Poaceae) in Eurasia. Plant Systematics and Evolution 308: 9.   DOI
10 Jang, T.-S., J. S. Parker, K. Emadzade, E. M. Temsch, A. R. Leitch and H. Weiss-Schneeweiss. 2018. Multiple origins and nested cycles of hybridization result in high tetraploid diversity in the monocot Prospero. Frontiers in Plant Science 9: 433.   DOI
11 Choi, B., H. Weiss-Schneeweiss, E. M. Temsch, S. So, H.-H. Myeong and T.-S. Jang. 2020. Genome size and chromosome number evolution in Korean Iris L. species (Iridaceae Juss.). Plants 9: 1284.   DOI
12 Becher, H., R. F. Powell, M. R. Brown, C. Metherell, J. Pellicer, I. J. Leitch and A. D. Twyford. 2021. The nature of intraspecific and interspecific genome size variation in taxonomically complex eyebrights. Annals of Botany 128: 639-651.   DOI
13 Bennett, M. D., P. Bhandol and I. J. Leitch. 2000. Nuclear DNA amounts in angiosperms and their modern uses-807 new estimates. Annals of Botany 86: 859-909.   DOI
14 Chang, C.-S. 2007. Ericaceae Juss. In The Genera of Vascular Plants of Korea. Park. C. W. (ed.), Academy Publishing Co., Seoul. Pp. 464-472.
15 Choi, B., G.-H. Gang, H. Kim, H. Byun, M. Kwak, S. So, H.-H. Myeong and T.-S. Jang. 2021. Cytological study of Cypripedium japonicum Thunb. (Orchidaceae Juss.): An endangered species from Korea. Plants 10: 1978.   DOI
16 Chung, K.-S. and G. Y. Chung. 2021. Chromosome numbers of eight Carex taxa in Korea (Cyperaceae). Korean Journal of Plant Taxonomy 51: 192-197.   DOI
17 Emadzade, K., T.-S. Jang, J. Macas, A. Kovarik, P. Novak, J. Parker and H. Weiss-Schneeweiss. 2014. Differential amplification of satellite PaB6 in chromosomally hypervariable Prospero autumnale complex (Hyacinthaceae). Annals of Botany 114: 1597-1608.   DOI
18 Choi, Y., B. Choi and T.-S. Jang. 2022. New chromosome counts in Juncus (Juncaceae) taxa from Korea. Cytologia (in press).
19 Temsch, E. M., P. Koutecky, T. Urfus, P. Smarda and J. Dolezel. 2021. Reference standards for flow cytometric estimation of abSolute Nuclear Dna Content In Plants. Cytometry Part A. Advanced Online Publication. Https://doi.org/10.1002/cyto.a.24495   DOI
20 Bou Dagher-Kharrat, M., N. Abdel-Samad, B. Douaihy, M. Bourge, A. Fridlender, S. Siljak-Yakovlev and S. C. Brown. 2013. Nuclear DNA C-values for biodiversity screening: Case of the Lebanese flora. Plant Biosystems 147: 1228-1237.   DOI
21 Yang, J.-C., Y.-H. Kwon, S.-J. Ji and C.-H. Shin. 2015. A new record of Rhododendron keiskei Miq. var. hypoglaucum Suto & Suzuki (Ericaceae) in Korea. Korean Journal of Plant Taxonomy 45: 239-242.   DOI
22 Stepanov, N. V. 1994. Chromosome numbers of some higher plants taxa of the flora of Krasnoyarsk region. Botanicheskii Zhurnal Moscow & Leningrad (St. Petersburg) 79: 135-139.
23 Temsch, E. M., J. Greilhuber and R. Krisai. 2010. Genome size in liverworts. Preslia 82: 63-80.
24 Weiss-Schneeweiss, H., A. R. Leitch, J. McCann, T.-S. Jang and J. Macas. 2015. Employing next generation sequencing to explore the repeat landscape of the plant genome. In Next Generation Sequencing in Plant Systematics. Regnum Vegetabile. Horandl, E. and M. Appelhans (eds.), Koeltz Scientific Books, Konigstein. Pp. 155-179.
25 Husband, B. C., S. J. Baldwin and J. Suda. 2013. The incidence of polyploidy in natural plant populations: Major patterns and evolutionary processes. In Plant Genome Diversity. Vol. 2. Physical Structure, Behaviour and Evolution of Plant Genomes. Leitch, I. J., J. Greilhuber, J. Dolezel and J. F. Wendel (eds.), Springer, Vienna. Pp. 255-276.
26 Ikeda, H., B.-M. Nam, N. Yamamoto, H. Funakoshi, A. Takano and H.-T. Im. 2021. Chromosome number of myoga ginger (Zingiber mioga: Zingiberaceae) in Korea. Korean Journal of Plant Taxonomy 51: 100-102.   DOI
27 Jang, T.-S., K. Emadzade, J. Parker, E. M. Temsch, A. R. Leitch, F. Speta and H. Weiss-Schneeweiss. 2013. Chromosomal diversification and karyotype evolution of diploids in the cytologically diverse genus Prospero (Hyacinthaceae). BMC Evolutionary Biolology 13: 136.   DOI
28 Jang, T.-S., J. McCann, J. S. Parker, K. Takayama, S.-P. Hong, G. M. Schneeweiss and H. Weiss-Schneeweiss. 2016. rDNA loci evolution in the genus Glechoma (Lamiaceae). PLoS ONE 11: e0167177.   DOI
29 Weiss-Schneeweiss, H. and G. M. Schneeweiss. 2013. Karyotype diversity and evolutionary trends in angiosperms. In Plant Genome Diversity. Vol. 2. Physical Structure, Behaviour and Evolution of Plant Genomes. Leitch, I. J., J. Greilhuber, J. Dolezel and J. F. Wendel (eds.), Springer, Vienna. Pp. 209-230.
30 Chung, K.-S. and H.-T. Im. 2020. Chromosome number report of three Carex sect. Mitratae taxa (Cyperaceae) in Korea. Korean Journal of Plant Taxonomy 50: 361-367.   DOI
31 Murin, A., I. Haberova and C. Zamsran. 1984. Further karyological studies of the Mongolian flora. Folia Geobotanica et Phytotaxonomica 19: 29-39.   DOI
32 Otto, S. P. and J. Whitton. 2000. Polyploid incidence and evolution. Annual Review of Genetics 34: 401-437.   DOI
33 Rice, A., L. Glick, S. Abadi, M. Einhorn, N. M. Kopelman, A. Salman-Minkov, J. Mayzel, O. Chay and I. Mayrose. 2015. The chromosome counts database (CCDB): A community resource of plant chromosome numbers. New Phytologist 206: 19-26.   DOI
34 Sax, K. 1930. Chromosome stability in the genus Rhododendron. American Journal of Botany 17: 247-251.   DOI
35 Fang, M. Y., R. C. Fang, M. Y. He, L. C. Hu, H. P. Yang and D. F. Chamberlain. 2005. Rhododendron. In Flora of China, Vol. 14. Wu, Z. Y. and P. H. Raven (eds.), Science Press, Beijing and Missouri Botanical Garden, St. Louis, MO. Pp. 260-455.
36 Greilhuber, J. 2005. Intraspecific variation in genome size in angiosperms: Identifying its existence. Annals of Botany 95: 91-98.   DOI
37 Gurzenkov, N. N. 1973. Studies of chromosome numbers of plants from the south of the Soviet Far East. Komarov Lectures 20: 47-61.
38 Schubert, I. 2007. Chromosome evolution. Current Opinion in Plant Biology 10: 109-115.   DOI
39 Sliwinska, E., J. Loureiro, I. J. Leitch, P. Smarda, J. Bainard, P. Bures, Z. Chumova, L. Horova, P. Koutecky, M. Lucanova, P. Travnicek and D. W. Galbraith. 2021. Application-based guidelines for best practices in plant flow cytometry. Cytometry Part A. Advanced online publication. https://doi.org/10.1002/cyto.a.24499.   DOI
40 Soltis, P. S. and D. E. Soltis. 2009. The role of hybridization in plant speciation. Annual Review of Plant Biology 60: 561-588.   DOI
41 Pellicer, J. and I. J. Leitch. 2020. The plant DNA C-values database (release 7.1): An updated online repository of plant genome size data for comparative studies. New Phytologist 226: 301-305.   DOI
42 Khan, G., J. Nolzen, H. Schepker and D. C. Albach. 2021. Incongruent phylogenies and their implications for the study of diversification, taxonomy, and genome size evolution of Rhododendron. American Journal of Botany 108: 1957-1981.   DOI
43 McCann, J., T.-S. Jang, J. Macas, G. M. Schneeweiss, N. J. Matzke, P. Novak, T. F. Stuessy, J. L. Villasenor and H. Weiss-Schneeweiss. 2018. Dating the species network: Allopolyploidy and repetitive DNA evolution in American daisies (Melampodium sect. Melampodium, Asteraceae). Systematic Biology 67: 1010-1024.   DOI
44 Mitrenina, E. Y., A. S. Erst, L. Peruzzi, M. V. Skaptsov, H. Ikeda, V. Y. Nikulin and W. Wang. 2021. Karyotype and genome size variation in white-flowered Eranthis sect. Shibateranthis (Ranunculaceae). PhytoKeys 187: 207-227.   DOI