The Journal of the Korean Society for Microbiology
/
v.3
no.1
/
pp.15-23
/
1968
For the effective control of Syphilis, many investigators have developed a more rapid, simple and economical screening serological test which is adequately sensitive and specific. To fulfill the requirements of a more rapid serologic test for syphlis, a substitute for the conventional serum specimen was needed since considerable time and labor are involved in the processing of blood to serum. Burdon suggested the use of plasma in the serologic tests for syphilis as a substitute for serum. He noticed that plasma was more sensitive than serum in the Kline and Kahn tests, and attributed this to the presence of more antibody-like substance, "reagin" in plasma than in serum. However, to make plasma sufficiently sensitive, it was necessary to inactivate plasma by heating at a temperature of $56^{\circ}C$ for about 30 minutes. Heating of plasma resulted in the precipitation of fibrinogen which made centrifugation necessary to obtain dear plasma. Since the chief disadvantage to the use of unheated plasma(or serum) was a reduction in sensitivity of results-which probably was due to a labile factor such as complement-Portnoy et al began to consider rapid chemical methods of inactivation of plasma(or serum). They experienced that choline chloirde was shown to be anticomplementary which suggested its use as an inactivating agent for unheated plasma(or serum). In 1959 Portnoy et al reported the Rapid Plasma Reagin(RPR) Test for syphilis which is a more rapid, economical and simple. But still this test has many disadvantages as a rapid performing, field and office procedure, because it requires the usual laboratory equipments such as centrifuge, rotating machine, microscope etc. To substitute these disadvantages of the RPR test, in 1962, Portnoy et al developed the Rapid Plasma Reagin(RPR) card test for syphilis, which has the following advantages: a) Simplicity and rapidity of performance, b) Requires no laboratory equipments, c) Stable antigen suspension, d) Adequate sensitivity and specificity. This RPR card test can be used as a rapidly performing and screening test in field investigation, outpatient clinics, small laboratories and hospitals doing limited syphilis serology, and predonor in blood bank. Private clinic which has limited laboratory equipment and technic for syphilis serology can also use this RPR card test as a tool in the rapid diagnosis of syphilis. It was thought that this RPR card test is a useful tool in Korea for private physician and mass survey for syphilis diagnosis. But Portnoy patented the reagents needed for the performing the RPR card test. Therefore authors developed newly the reagents and according to Portnoy's method evaluated the newly developed. RPR card test compared with the VDRL, Kolmer CF, and RPCF tests. The RPR card and VDRL tests were performed plasma and serum from the total 1,132 cases. Among these 1,131 cases, 521 were syphilis suspected laboratory specimens, and 611 were syphilis unsuspected healthy young men. After screening with these two tests, the RPR card and VDRL tests, reactive specimens to the above one or both tests were retested by the Kolmer CF and RPCF tests.
This experiment used chlorophyll fluorescence techniques to assess the effect of irradiant during leaf development on photoinhibition of photosynthesis in Panax ginseng. Seedlings of p. ginseng were grown in the 91asshouse at four shade levels. The maximum mid-day irradiant in each treatment between emergence (January 4) and completion of the experiment (February 25) was 1220, 485, 235, 125 $\mu$mol/$\textrm{m}^2$/s. To assess the rapidity of photosynthetic readaptation to changes in light levels, fluorescence parameters (Fo, F, Fm, Fm', AF/Fm;, Fv/Fm) were measured for three days before and after transfer of plants (on February 21) from each light treatment into each of the other light treatments. Before transfer, dark adapted values of Fv/Fm in the 1220 (0.699) and 485 (0.739) treatments were different from each other and lower than values in the 235 (0.764) and 125 (0.768) treatments, indicating mild photoinhibition. Patterns of change in F during the day also differed between treatments, with low light treatments tracking irradiant levels, but F in the high light treatment (1220) declined in the morning, presumably due to fluorescence quenching. Although plants grown at high irradiant had relatively low photosynthetic efficiency, relative electron transport rate was greater than in lower irradiant treatments. After transfer, plants adopted the daily pattern of change in F of the treatment to which they were moved with little change in absolute levels of F, except in plants transferred from the highest (1220) to the lowest light level (125), where F increased over the course of the three days following transfer. After plants were transferred, Fm' converged on values similar to those in plants raised in the treatments to which they were moved. Values of Fv/Fm in plants moved from low to high light declined dramatically, but there was no decline in plants from 485 moved to 1220. Values of Pv/Fm in plants that were moved from high light to lower light increased to values above those recorded in plants raised in the lower light treatments. Reductions in quantum efficiency caused by photoinhibition at high irradiant may be more than compensated for by higher electron transport rates, although evidence suggests that under high irradiant this tends to be balanced by reduced leaf area and earlier senescence. Chlorophyll fluorescence techniques appear capable of indicating effects of irradiant induced stress in ginseng, yielding results comparable to those obtained with gas exchange techniques but in less time and with greater replication.
Social commerce is a new internet business model which are types of joint purchase, social link, promotion, and on-off association etc. The recent public organization has the dual target to increase sound finance, and to improve customer satisfaction and the quality of public service. The purpose of this study is to propose the strategy of public social commerce which makes it's customers become positive purchasers. We analyzed 31 public organizations, and found that the type of social link interlocking with SNS site in the various purpose of public, and the offline association type using the service of location base would be utilized very highly. We also found that the joint purchase type and the promotion type would be used in the area of public and private selective services intending to make a profit. The anticipated performance are as follows: rapidity and reliance, customer made thing and goodwill, convenience for the service environment quality, publicity and diffusion, and sales promotion, productivity increase and new finding of revenue model for the profit increase outcome.
Gwon-Shik Ihn;Bong-Weon Kim;Sohn Moo-Jeong;Ihn-Tak Kim
Journal of the Korean Chemical Society
/
v.32
no.4
/
pp.323-332
/
1988
The bacteria containing urease convert each molecule of urea into two molecules of ammonia and one molecule of carbon dioxide gas. Bacterial electrodes have been constructed by immobilizing the Proteus vulgaris on an ammonia and a carbon dioxide gas-sensors, and were investigated for the effects of pH, temperature, buffer solution, bacterial amounts and interferences, and life time. NH3-bacterial electrode based on ammonia gas-sensor had linearity in the range of $7.0{\times}10^{-4}\;-\;3.0{\times}10^{-2}$M urea in pH 7.4, 0.05M phosphate buffer at $25^{\circ}C$ with a slope of 116.7 mV/decade. While $CO_{2-}$bacterial electrode based on carbon dioxide gas-sensor bad linearity in the range of $7.0{\times}10^{-4}\;-\;5. 0{\times}10^{-2}$M urea in pH 7.0, 0.1M phosphate buffer at $30^{\circ}C$with a slope of $45.4{\times}45.7mV/decade$. As the clinical application, urea in urine was determined by these devices and this result was compared with spectrophotometric method. Consequently, these electrodes could be used for the analysis of many samples because of simplicity, rapidity and convenience of the experimental procedure.
Journal of the Society of Cosmetic Scientists of Korea
/
v.34
no.1
/
pp.57-62
/
2008
No matter how small amount of heavy metals it may be cause skin allergies through percutaneous adsorption when existing in cosmetic products as impurities. In order to develop a highly sensitive method for simultaneous determination of $Pb^{2+},\;Fe^{2+},\;Cu^{2+},\;Ni^{2+},\;Zn^{2+},\;Co^{2+},\;Cd^{2+},\;and\;Mn^{2+}$ in coloring agents and cosmetic products with rapidity and accuracy, we carried out the determination on ion chromatography. All of these metals are well separated through a bifunctional ion-exchange column(IonPac CS5A) and detected by post-column reaction and spectrophotometric detection. The calibration graphs are linear($r^2>0.999$) in the range $0.1{\sim}1000{\mu}g/mL$. Detection limits for 200 ${\mu}L$ of sample solution are at the level of ${\mu}g/L$, which is sufficient for judging whether the product is safe or not. The relative standard deviations(RSDs) of the retention time and the peak area are less than 0.21 and 1.24%, respectively. The recovery rates are $97{\sim}104%$. The new method was applied to analyze the amount of heavy metals which were contained in 22 cosmetic products and 11 coloring agents.
Fresh cut vegetables provide convenience and rapidity to consumers. However, they have a weakness with respect to their short shelf-life due to browning and quality degradation via increased respiration. To overcome this problem, the effect of packaging film on the short-term storage of cut kimchi cabbages was investigated. Polypropylene (PP), oriented polypropylene (OPP), and low-density polyethylene (LDPE) films were used as packaging film, and cut kimchi cabbages were stored in the packaging films at $5^{\circ}C$ for 4 weeks. PP film packaging showed the least weight loss and soluble solids loss after 4 weeks. Titratable acidity increased during storage for all samples, however, the increase rate of titratable acidity in PP and OPP film packaging decreased, which was lower than that of LDPE film packaging. Color values decreased over time during storage. In appearance, PP film packaging was better than other films due to their high transparency. In a sensory test, there was no statistical difference among samples. Taken together, the transparent PP film packaging was more effective for short-term storage of cut kimchi cabbages. Thus, this study provides useful information for the selection of packaging materials for cut kimchi cabbage marketing.
Kim, Kyu-Il;Choi, Sang-Soo;Park, Hark-Soo;Ko, Sang-Jun;Song, Jung-Suk
Journal of the Korea Institute of Information Security & Cryptology
/
v.24
no.5
/
pp.871-883
/
2014
New types of attacks that mainly compromise the public, portal and financial websites for the purpose of economic profit or national confusion are being emerged and evolved. In addition, in case of 'drive by download' attack, if a host just visits the compromised websites, then the host is infected by a malware. Website falsification detection system is one of the most powerful solutions to cope with such cyber threats that try to attack the websites. Many domestic CERTs including NCSC (National Cyber Security Center) that carry out security monitoring and response service deploy it into the target organizations. However, the existing techniques for the website falsification detection system have practical problems in that their time complexity is high and the detection accuracy is not high. In this paper, we propose website falsification detection system based on image and code analysis for improving the performance of the security monitoring and response service in CERTs. The proposed system focuses on improvement of the accuracy as well as the rapidity in detecting falsification of the target websites.
This study was conducted to develop a sensitized latex-antigen for serodiagnosis of toxoplasmosis in animals. Tachyzoites of T gondii(RH-strain) harvested from mouse peritoneal cavity were purified through the filtraton of polycarbonate membrane(pore size, $3.0{{\mu}m}$, Costar Co.) and disrupted by ultrasonicator. The tachyzoite suspension was ultracentrifuged for 30 min at $60,000{\times}g(4{^{\circ}C})$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $0.8{{\mu}m}$ in diameter(Sigma) were used for the preparation of sensitized latex-antigen suspension. The several parameters including the preparation conditions, incubation buffer. serum dilution buffer and stability of agglutination reactions were evaluated and the results obtained were summarized as follows : 1. The antigen consisting of a water-lysate of T gondii tachyzoites was adsorbed onto polystyrene latex particles of $0.8{{\mu}m}$ in diameter by adding a latex suspension to an equal volume of diluted antigen solution and by incubating the mixture at $37{^{\circ}C}$ under different conditions. 2. The optimum incubation buffer used for the antigen sensitization was 0.1M Tris-HCl buffer(pH 8.0). 3. The optimum serum dilution buffer used for the latex agglutination test was 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300 mM NaCl. But 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300-600 mM NaCl, 0.5% BSA and 0.01% Tween-20 improved the agglutination pattems and cleared the background of microplate well without the effects on L.A titer. 4. The time required for antigen sensitization was 40 and 60 min in incubation buffer(pH 8.0) at $37{^{\circ}C}$. But the optimun time for antigen sensitization was min at $37{^{\circ}C}$. 5. The optimun quantity of antigen absorbed on latex particles for proper agglutination was the range of 20 to $32{\mu}g$ of latex particles. 6. The optimun concentration of the latex-antigen suspension for the proper agglutination reaction was determined as 0.2%(w/v). 7. The specificity, rapidity and simplicity of the latex-particle agglutination test suggested that it might be adaptable to large scale serum screening.
Park, Eui-Seob;Jung, Yong-Bok;Ryu, Chang-Ha;SunWoo, Choon;Choi, Yong-Kun;Heo, Sung;Cheon, Dae-Sung
Tunnel and Underground Space
/
v.17
no.3
s.68
/
pp.165-174
/
2007
The technique, which reproduces the figures of objects from measured data of the objects using 3-D laser scanner, is called reverse engineering. Recently, research studies into applications of reverse engineering to rock engineering are increasing in number, in the discontinuity surveys for rock slopes out of man's reach, or rapid discontinuity surveys for wide range areas. For analysis of discontinuity using reverse engineering, a program for processing point clouds data from the 3-D laser scanner, for sampling from these point clouds data, and finally analyzing the discontinuity is needed. However, existing programs rarely have sufficient functions to properly analyze the discontinuities. In this study, a program was developed, which can automatically sample discontinuities from the point clouds data which measured in a rock slope using a 3-D laser scanner, and which can also undertake statistical analysis of the discontinuities. This developed program was verified by the application of discontinuity surveys in a rock slope and a tunnel. By undertaking the discontinuity survey using a 3-D laser scanner and the developed program, the feasibility and rapidity of such surveys is expected to improve in areas out of man's reach in geotechnical surveys. Taking into consideration the fact that the international level of related techniques is at a rudimentary stage, the possibility of prior occupation of a broad market is also expected.
Journal of the Korea Academia-Industrial cooperation Society
/
v.20
no.5
/
pp.27-35
/
2019
The purpose of this study is to analyze the displacement characteristics in slow-moving landslide area using digital elevation model and airborne LiDAR when unpredictable disaster such as slow-moving landslide occurred. We also aimed to provide basic data for establishing a rapid, reasonable and effective restoration plan. In this study, slow-moving landslide occurrence cracks were selected through the airborne LiDAR data, and the topographic changes and the scale of occurrence were quantitatively analyzed. As a result of the analysis, the study area showed horseshoe shape similar to the general form of slow-moving landslide occurrence in Korea, and the direction of movement was in the north direction. The total area of slow-moving landslide damage was estimated to about 2.5ha, length of landsldie scrap 327.3m, average width 19.3m, and average depth 8.6m. The slow-moving landslides did not occur on a large scale but occurred on the adjacent slope where roads were located, caused damage to retaining walls and roads. The field survey of slow-moving landslides was limited by accessibility and safety issues, but there was an advantage that accurate analysis was possible through the airborne LiDAR. However, because airborne LiDAR has costly disadvantages, it has proposed a technique to mount LiDAR on UAV for rapidity, long-term monitoring. In a slow-moving landslide damage area, information such as direction of movement of cracks and change of scale should be acquired continuously to be used in restoration planning and prevention of damage.
본 웹사이트에 게시된 이메일 주소가 전자우편 수집 프로그램이나
그 밖의 기술적 장치를 이용하여 무단으로 수집되는 것을 거부하며,
이를 위반시 정보통신망법에 의해 형사 처벌됨을 유념하시기 바랍니다.
[게시일 2004년 10월 1일]
이용약관
제 1 장 총칙
제 1 조 (목적)
이 이용약관은 KoreaScience 홈페이지(이하 “당 사이트”)에서 제공하는 인터넷 서비스(이하 '서비스')의 가입조건 및 이용에 관한 제반 사항과 기타 필요한 사항을 구체적으로 규정함을 목적으로 합니다.
제 2 조 (용어의 정의)
① "이용자"라 함은 당 사이트에 접속하여 이 약관에 따라 당 사이트가 제공하는 서비스를 받는 회원 및 비회원을
말합니다.
② "회원"이라 함은 서비스를 이용하기 위하여 당 사이트에 개인정보를 제공하여 아이디(ID)와 비밀번호를 부여
받은 자를 말합니다.
③ "회원 아이디(ID)"라 함은 회원의 식별 및 서비스 이용을 위하여 자신이 선정한 문자 및 숫자의 조합을
말합니다.
④ "비밀번호(패스워드)"라 함은 회원이 자신의 비밀보호를 위하여 선정한 문자 및 숫자의 조합을 말합니다.
제 3 조 (이용약관의 효력 및 변경)
① 이 약관은 당 사이트에 게시하거나 기타의 방법으로 회원에게 공지함으로써 효력이 발생합니다.
② 당 사이트는 이 약관을 개정할 경우에 적용일자 및 개정사유를 명시하여 현행 약관과 함께 당 사이트의
초기화면에 그 적용일자 7일 이전부터 적용일자 전일까지 공지합니다. 다만, 회원에게 불리하게 약관내용을
변경하는 경우에는 최소한 30일 이상의 사전 유예기간을 두고 공지합니다. 이 경우 당 사이트는 개정 전
내용과 개정 후 내용을 명확하게 비교하여 이용자가 알기 쉽도록 표시합니다.
제 4 조(약관 외 준칙)
① 이 약관은 당 사이트가 제공하는 서비스에 관한 이용안내와 함께 적용됩니다.
② 이 약관에 명시되지 아니한 사항은 관계법령의 규정이 적용됩니다.
제 2 장 이용계약의 체결
제 5 조 (이용계약의 성립 등)
① 이용계약은 이용고객이 당 사이트가 정한 약관에 「동의합니다」를 선택하고, 당 사이트가 정한
온라인신청양식을 작성하여 서비스 이용을 신청한 후, 당 사이트가 이를 승낙함으로써 성립합니다.
② 제1항의 승낙은 당 사이트가 제공하는 과학기술정보검색, 맞춤정보, 서지정보 등 다른 서비스의 이용승낙을
포함합니다.
제 6 조 (회원가입)
서비스를 이용하고자 하는 고객은 당 사이트에서 정한 회원가입양식에 개인정보를 기재하여 가입을 하여야 합니다.
제 7 조 (개인정보의 보호 및 사용)
당 사이트는 관계법령이 정하는 바에 따라 회원 등록정보를 포함한 회원의 개인정보를 보호하기 위해 노력합니다. 회원 개인정보의 보호 및 사용에 대해서는 관련법령 및 당 사이트의 개인정보 보호정책이 적용됩니다.
제 8 조 (이용 신청의 승낙과 제한)
① 당 사이트는 제6조의 규정에 의한 이용신청고객에 대하여 서비스 이용을 승낙합니다.
② 당 사이트는 아래사항에 해당하는 경우에 대해서 승낙하지 아니 합니다.
- 이용계약 신청서의 내용을 허위로 기재한 경우
- 기타 규정한 제반사항을 위반하며 신청하는 경우
제 9 조 (회원 ID 부여 및 변경 등)
① 당 사이트는 이용고객에 대하여 약관에 정하는 바에 따라 자신이 선정한 회원 ID를 부여합니다.
② 회원 ID는 원칙적으로 변경이 불가하며 부득이한 사유로 인하여 변경 하고자 하는 경우에는 해당 ID를
해지하고 재가입해야 합니다.
③ 기타 회원 개인정보 관리 및 변경 등에 관한 사항은 서비스별 안내에 정하는 바에 의합니다.
제 3 장 계약 당사자의 의무
제 10 조 (KISTI의 의무)
① 당 사이트는 이용고객이 희망한 서비스 제공 개시일에 특별한 사정이 없는 한 서비스를 이용할 수 있도록
하여야 합니다.
② 당 사이트는 개인정보 보호를 위해 보안시스템을 구축하며 개인정보 보호정책을 공시하고 준수합니다.
③ 당 사이트는 회원으로부터 제기되는 의견이나 불만이 정당하다고 객관적으로 인정될 경우에는 적절한 절차를
거쳐 즉시 처리하여야 합니다. 다만, 즉시 처리가 곤란한 경우는 회원에게 그 사유와 처리일정을 통보하여야
합니다.
제 11 조 (회원의 의무)
① 이용자는 회원가입 신청 또는 회원정보 변경 시 실명으로 모든 사항을 사실에 근거하여 작성하여야 하며,
허위 또는 타인의 정보를 등록할 경우 일체의 권리를 주장할 수 없습니다.
② 당 사이트가 관계법령 및 개인정보 보호정책에 의거하여 그 책임을 지는 경우를 제외하고 회원에게 부여된
ID의 비밀번호 관리소홀, 부정사용에 의하여 발생하는 모든 결과에 대한 책임은 회원에게 있습니다.
③ 회원은 당 사이트 및 제 3자의 지적 재산권을 침해해서는 안 됩니다.
제 4 장 서비스의 이용
제 12 조 (서비스 이용 시간)
① 서비스 이용은 당 사이트의 업무상 또는 기술상 특별한 지장이 없는 한 연중무휴, 1일 24시간 운영을
원칙으로 합니다. 단, 당 사이트는 시스템 정기점검, 증설 및 교체를 위해 당 사이트가 정한 날이나 시간에
서비스를 일시 중단할 수 있으며, 예정되어 있는 작업으로 인한 서비스 일시중단은 당 사이트 홈페이지를
통해 사전에 공지합니다.
② 당 사이트는 서비스를 특정범위로 분할하여 각 범위별로 이용가능시간을 별도로 지정할 수 있습니다. 다만
이 경우 그 내용을 공지합니다.
제 13 조 (홈페이지 저작권)
① NDSL에서 제공하는 모든 저작물의 저작권은 원저작자에게 있으며, KISTI는 복제/배포/전송권을 확보하고
있습니다.
② NDSL에서 제공하는 콘텐츠를 상업적 및 기타 영리목적으로 복제/배포/전송할 경우 사전에 KISTI의 허락을
받아야 합니다.
③ NDSL에서 제공하는 콘텐츠를 보도, 비평, 교육, 연구 등을 위하여 정당한 범위 안에서 공정한 관행에
합치되게 인용할 수 있습니다.
④ NDSL에서 제공하는 콘텐츠를 무단 복제, 전송, 배포 기타 저작권법에 위반되는 방법으로 이용할 경우
저작권법 제136조에 따라 5년 이하의 징역 또는 5천만 원 이하의 벌금에 처해질 수 있습니다.
제 14 조 (유료서비스)
① 당 사이트 및 협력기관이 정한 유료서비스(원문복사 등)는 별도로 정해진 바에 따르며, 변경사항은 시행 전에
당 사이트 홈페이지를 통하여 회원에게 공지합니다.
② 유료서비스를 이용하려는 회원은 정해진 요금체계에 따라 요금을 납부해야 합니다.
제 5 장 계약 해지 및 이용 제한
제 15 조 (계약 해지)
회원이 이용계약을 해지하고자 하는 때에는 [가입해지] 메뉴를 이용해 직접 해지해야 합니다.
제 16 조 (서비스 이용제한)
① 당 사이트는 회원이 서비스 이용내용에 있어서 본 약관 제 11조 내용을 위반하거나, 다음 각 호에 해당하는
경우 서비스 이용을 제한할 수 있습니다.
- 2년 이상 서비스를 이용한 적이 없는 경우
- 기타 정상적인 서비스 운영에 방해가 될 경우
② 상기 이용제한 규정에 따라 서비스를 이용하는 회원에게 서비스 이용에 대하여 별도 공지 없이 서비스 이용의
일시정지, 이용계약 해지 할 수 있습니다.
제 17 조 (전자우편주소 수집 금지)
회원은 전자우편주소 추출기 등을 이용하여 전자우편주소를 수집 또는 제3자에게 제공할 수 없습니다.
제 6 장 손해배상 및 기타사항
제 18 조 (손해배상)
당 사이트는 무료로 제공되는 서비스와 관련하여 회원에게 어떠한 손해가 발생하더라도 당 사이트가 고의 또는 과실로 인한 손해발생을 제외하고는 이에 대하여 책임을 부담하지 아니합니다.
제 19 조 (관할 법원)
서비스 이용으로 발생한 분쟁에 대해 소송이 제기되는 경우 민사 소송법상의 관할 법원에 제기합니다.
[부 칙]
1. (시행일) 이 약관은 2016년 9월 5일부터 적용되며, 종전 약관은 본 약관으로 대체되며, 개정된 약관의 적용일 이전 가입자도 개정된 약관의 적용을 받습니다.