• Title/Summary/Keyword: RapidForm

Search Result 966, Processing Time 0.027 seconds

Rapid Purification of Recombinant Human Lipocortin-I Secreted from Saccharomyces cerevisiae

  • Chung, Bong-Hyun;Nam, Soo-Wan
    • Biotechnology and Bioprocess Engineering:BBE
    • /
    • v.5 no.4
    • /
    • pp.242-246
    • /
    • 2000
  • Human lipocortin-I was expressed as a secretory product by Saccharomyces cerevisiae harboring an expression system consisting of GAL10 promoter, inulinase signal sequence and lipocortin-I terminator. Fed-batch fermentation was carried out to overproduce recombinant human lipocortin-I. The culture medium was desalted and concentrated by ultrafiltration, and then subjected to hydroxyapatite column chromatography. The lipocortin-I was purified to >98% purity by single-step hydroxyapatite column chromato-graphy. However, it was found that the purified lipocortin-I was a proteolytically-cleaved form which was cleaved immediately after the basic amino acid Lys26.

  • PDF

DNA Profiles Analysed by Polymerase Chain Reaction-Random Amplified Polymorphic DNAs in Shortnecked Clam (Ruditapes philippinarum) Populations

  • Yoon, Jong-Man;Kim, Yong-Ho;Kim, Jong-Yeon
    • Proceedings of the Korean Aquaculture Society Conference
    • /
    • 2002.08a
    • /
    • pp.172-174
    • /
    • 2002
  • Out of 20 primers, 6 generated a total of 1,11 major and minor RAPD bands, producing approximately 4.2 average polymorphic bands pe primer in shortnecked clam (Ruditapes philippinarum) population from Anmyeondo. The bandsharing value altered form 0.15 to 0.74, with the average of 0.5, as calculated by bandsharing analysis. The RAPD profiles obtained with DNAs of two populations from Anmyeondo and Seocheon, respectively, were considerably different (0.20 and 0.51, respectively). The varying degrees of difference among populations may also be of relevance to the retricted hybridization of wild bivalve. Besides gene mapping and breeding applications, PCR-RAPD system could be very useful for the rapid certification and quality control of seed production and for every projects based on PCR amplification of specific bivalve DNA fragments.

  • PDF

A Study on Effects of Characteristics of Internet Shopping Mall on Customer Loyalty (인터넷쇼핑몰의 특성이 고객충성도에 미치는 영향에 관한 연구)

  • Choi Seung-Il
    • Proceedings of the Korea Contents Association Conference
    • /
    • 2005.05a
    • /
    • pp.295-299
    • /
    • 2005
  • The object of this study is the business form between the consumer and the enterprise having a rapid growth nowadays. We classified enterprises' characteristic factors in internet shopping mall into product's characteristic factors on shopping mall, characteristic factors of the system on shopping mall and characteristic factors of the interaction between customers and shopping mall in the internet shopping mall. And then we investigated how these classified characteristic factors on shopping mall could influence on customer loyalty.

  • PDF

Lymphomatosis Cerebri

  • Choi, Chan-Young;Lee, Chae-Heuck;Joo, Mee
    • Journal of Korean Neurosurgical Society
    • /
    • v.54 no.5
    • /
    • pp.420-422
    • /
    • 2013
  • Lymphomatosis cerebri is considered a diffuse form of primary central nervous system lymphoma and very rare. It is not well recognized and may be misdiagnosed with infiltrating tumors, degenerative disorders, ischemic diseases, and infectious diseases developed in the brain. Awareness of the possibility of this rare disease and early biopsy are required for differential diagnosis and preventing poor clinical outcomes. We report a case with lymphomatosis cerebri who presented with rapid neurological deteriorations and review the relevant literatures.

Implementation of Intrusion Detection System Using Packet Capture (패킷캡쳐를 이용한 침입탐지 시스템의 구현)

  • 김영진;한승조
    • Journal of the Korea Institute of Information and Communication Engineering
    • /
    • v.6 no.6
    • /
    • pp.833-842
    • /
    • 2002
  • Computer security is considered important due to the side effect generated from the expansion of computer network and rapid increase of use of computers. A attack of intruders using a vulnerability of operating system, protocol and application programs. And so, The attack methods is to be high technology and professional. Thus It must be necessity that we necessary a solution to structure, management for framework of information technology. This paper develope intrusion detecting system for separating intruders form critical system and design IDS model and implementation of it.

Analysis of Dynamic Chinese character Typography Design in Motion Graphics (모션그래픽에서 다이내한 중국문자의 타이포그래되에 대한분석)

  • Fujialun, Fujialun;Kim, Hav-Yoon
    • Proceedings of the Korea Contents Association Conference
    • /
    • 2014.11a
    • /
    • pp.329-330
    • /
    • 2014
  • The continuous development of information technology had led people into the era of new media. New technologies, in its rapid development, have also produced impacts on traditional visual communication design. As an integral element in traditional visual communication design, Chinese characters have no longer confined to traditional paper media but turned to dynamic video space. In light of the changing globalization tendency, dynamic Chinese characters are producing increasing influences in motion graphics. It is an inevitable trend in character typography design that visual communication will make a breakthrough in the traditional expression boundary. This paper analyzes the influence of dynamic Chinese characters upon motion graphics in view of typography and style, and puts forward with the typography design that can conform to the development of the time, spread information and express emotions in a better way.

  • PDF

The Pricing of Corporate Common Stock By OPM (OPM에 의한 주식가치(株式價値) 평가(評價))

  • Jung, Hyung-Chan
    • The Korean Journal of Financial Management
    • /
    • v.1 no.1
    • /
    • pp.133-149
    • /
    • 1985
  • The theory of option pricing has undergone rapid advances in recent years. Simultaneously, organized option markets have developed in the United States and Europe. The closed form solution for pricing options has only recently been developed, but its potential for application to problems in finance is tremendous. Almost all financial assets are really contingent claims. Especially, Black and Scholes(1973) suggest that the equity in a levered firm can be thought of as a call option. When shareholders issue bonds, it is equivalent to selling the assets of the firm to the bond holders in return for cash (the proceeds of the bond issues) and a call option. This paper takes the insight provided by Black and Scholes and shows how it may be applied to many of the traditional issues in corporate finance such as dividend policy, acquisitions and divestitures and capital structure. In this paper a combined capital asset pricing model (CAPM) and option pricing model (OPM) is considered and then applied to the derivation of equity value and its systematic risk. Essentially, this paper is an attempt to gain a clearer focus theoretically on the question of corporate stock risk and how the OPM adds to its understanding.

  • PDF

An Experimental Study on the Basic Properties of Lightweight Concrete Secondary Products by Admixture (혼화재료를 이용한 경량콘크리트 2차 제품의 제작을 위한 기초적 물성에 관한 실험적 연구)

  • 김영진;공민호;김광기;강태경;임남기;정상진
    • Proceedings of the Korea Concrete Institute Conference
    • /
    • 2003.05a
    • /
    • pp.775-780
    • /
    • 2003
  • The propose of this study is to discover concrete secondary products for proper mixing by lightweight concrete. The standard of water ratio 50% and weight substition 0%, 10%, 20% by Fly-ash. When produce manufactures, there use for maintain its form weight substition and addition among the viscosity agent each Silica-fume and Hydroxy propyl methyl cellulos. Testing method was to operate slump, air content, compressive strength test, rapid freezing and thawing test. The result of this study is appeared when substition Fly-ash generally it had better use Silica-fume.

  • PDF

A Condition Analysis and Response Measures of Cyberstalking (사이버스토킹의 실태 및 고찰에 관한 연구)

  • Kim, Tae-Hwan;Jung, Il-Seok
    • Journal of the Korean Society of Hazard Mitigation
    • /
    • v.4 no.3 s.14
    • /
    • pp.19-23
    • /
    • 2004
  • In current society, there has been rapid change in process towards the information society from the industrial society. Cyberstalking can be regarded as a kind of new comer in the issue of contemporary crimes. The Cyberstalking has been focused on famous people and entertainers in th past, whereas more seriously, the range of victims has expanded to the public in recent. Form this aspect, the goal of this study is establish the cyberstalking by considering causes, characteristics and suggest legal, social methods.

Evaluation of Planning Transparence of User Interface Reflecting State Schemas (스키마 개념을 도입한 사용자 계획수립의 용이도 평가)

  • ;Yoon, Wan Chul
    • Journal of the Korean Operations Research and Management Science Society
    • /
    • v.17 no.2
    • /
    • pp.45-54
    • /
    • 1992
  • It become increasingly important to design user interface to carry low complexity. The cognitive limitations of users severely restrict utility of highly intelligent but complex modern systems. Since humans are known to use schemas to reduce cognitive complexity, imposing good consistency to an interface design that may help that user form useful schemas will provide powerful control over the complexity. This present a research effort to develop a quantitative method for evaluating interface complexity that the user would experience planning his or her course of action. Taking into account the user's potential schemas, a quantitative measure based on information theory was develped to assess the navigational complexity. This approach does not rely on the subjective judgment of the researcher as most schemes dealing with user schemas do. The proposed method may benefit the rapid prototyping approach to design a better user interface by allowing handy assessment of the design.

  • PDF