• The Korean Journal of Physiology and Pharmacology
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• 제16권4호
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• pp.231-236
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• 2012

We studied the effects of acetylcholinesterase inhibitors, donepezil and galantamine, and an N-methyl-D-aspartate (NMDA) receptor blocker, memantine, on sleep-wake architecture in rats. Screw electrodes were chronically implanted into the frontal and parietal cortex for the electroencephalography (EEG). EEG was recorded with a bio-potential amplifier for 8 h from 09:30 to 17:30. Vibration was recorded to monitor animal activity with a vibration measuring device. Sleep-wake states such as wake (W), slow-wave sleep (S) and paradoxical or rapid eye movement sleep (P), were scored every 10 sec by an experimenter. We measured mean episode duration and number of episode to determine which factor sleep disturbance was attributed to. Donepezil and memantine showed a significant increase in total W duration and decreases in total S and P duration and delta activity. Memantine showed increases in sleep latency and motor activity. Changes of S and P duration in memantine were attributed from changes of mean episode duration. Galantamine had little effect on sleep architecture. From these results, it is showed that galantamine may be an anti-dementia drug that does not cause sleep disturbances and memantine may be a drug that causes severe sleep disturbance.

• 한국산학기술학회논문지
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• 제11권6호
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• pp.2013-2020
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• 2010

본 연구에서는 기존의 전형적인 토스터가 갖는 개방된 출입구로 인한 열손실 발생, 이물질의 투입, 빵을 구운 정도를 확인할 수 없음에 대한 소비자 불만족 등의 문제점을 해결하기 위해 새로운 방식의 토스터인 폴더형 캡을 갖는 투명 토스터에 대한 모델링을 연구하였다. 먼저 1차 모델링을 수행하였으며, 이어서 RP제작을 통하여 모델링에 서 확인하지 못한 문제점을 수정 및 보완하여 2차 모델링을 수행하였다. 2차 모델링에서는 메커니즘분석을 통해 홀더 간섭을 확인하여 수정하였고, 이어서 3D 모델링, 어셈블리, 시뮬레이션 분석을 통하여 폴더형 캡의 기능을 갖는 투명 토스터를 최종 모델링하였다. 본 연구에서 사용한 3D 모델링 툴은 Pro/Engineer 4.0이며, 설계된 모델링은 폴더형 캡 의 기능을 갖추어 열손실을 최소화하고, 투명한 외부케이스로 구성되어 빵 굽는 과정을 육안으로 확인하게 함으로써 심미적 만족도를 향상시키는 기능을 갖춘 폴더형 캡의 투명 토스터이다.

• 대한치과의사협회지
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• 제46권9호
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• pp.563-573
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• 2008

Purpose : The purpose of this study is to investigate the effects of Simvastain, which is HMG-CoA reductase inhibitor, on proliferation and differentiation of osteoblast. Materials & Methods : Twenty-four cell culture plates containing essential medium were seeded with UMR-106 cell lines, at density of 5 x $10^4$ cells per plate. Each plates were incubated with 5% $CO^2$incubator $37^{\circ}C$. Starting from 2 days after incubation, cell culture medias were replaced with Osteogenesis induction media every 2 days, for 12 days. In some plates, 0.01, 0.1, 1, 10, $100\muM$ of Simvastatin were added with Osteogenesis induction media, and classified as "test group". Those not added with Simvastatin were classified as "control group". Results : 1. When Alrizarin Red staining was observed with naked eye, control group showed normal deep red color, but test group show rapid decrease of red color as Simvastatin concentration increased more than $0.1\muM$. 2, When observed with microscope, compared to control group, amount of osteo matrix stained with Alrizarin Red decreased rapidly in Simvastatin concentration more than $0.1\muM$. 3. In optical density analysis, regarding control group as a basis, mineral deposition decreased rapidly when Simvastatin concentration increased more than $0.1\muM$. 4. In flow cytometry analysis, survival rate of UMR-106 cell showed no changes in both control group and test group. Conclusion : From the above results, we were able to identify that Simvastatin inhibited osteogenesis without effecting survival or cell number of osteoblasts.

• 한국수산과학회지
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• 제53권4호
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• pp.572-576
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• 2020

As grouper aquaculture production intensifies worldwide, hybrids are increasingly produced to induce heterosis of traits such as rapid growth and strong environmental adaptability. In this study, we compared the morphological characteristics of a hybrid grouper to those of its parent species Epinephelus moara and E. lanceolatus. The coefficients of external and skeletal traits evaluated using soft X-rays did not differ significantly among groups; however, several patterns were detected among measured traits. The body depth (BD), length of caudal peduncle (LCP), and pre-anal fin length (PAL) of the hybrid were the same as those of E. moara, whereas the pre-pectoral fin length (PPL) and interorbital width (IOW) of the hybrid were the same as those of E. lanceolatus. Traits that did not differ significantly among the three groups included pre-dorsal fin length (PDL), pre-ventral fin length (PVL), length of anal fin (LAF), and length of dorsal fin (LDF), whereas the depth of caudal peduncle (DCP) and snout length (SNL) showed intermediate traits, and eye diameter (ED) was significantly smaller in the hybrid. Therefore, intermediate traits such as DCP and SNL and specific traits such as ED may be used as indices for hybrid classification.

• 한국균학회지
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• 제14권4호
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• pp.265-271
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• 1986

털목이버섯의 저분자 독성물질로부터 다음과 같은 결론을 얻었다. 털목이버섯 열수추출액의 $LD_{50}$은 복강 및 경구투여시 각각 1.25와 4.18 g/kg이었다. 건조 털목이버섯 100 g에는 650$\sim$700 mouse lethal unit(=MLU)이 함유되어 있었다. 열수총추출액, C분흑 및 최종성분의 1 MLU는 각각 및 28.5, 25.0 및 12.0 mg/mouse이었다. 독성물질의 일반적일 증상은 행동둔화, 체모기립, 전율, 두부진동, 안구돌출, 사지마비, 사망직전의 급격한 다리운동 및 호흡마비 등이었다.

• The Journal of Korean Physical Therapy
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• 제20권1호
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• pp.67-73
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• 2008

Purpose: The purpose of this study was to investigate the effect of the intensity of microcurrent stimulation (with currents of 50 ${\mu}A$, 100 ${\mu}A$ and 300 ${\mu}A$ using a pulse frequency of 5 pps) on wound healing in rats. Methods: Sixty male Korean rats were randomly divided into four groups of 15 rats that were subjected to four different treatment protocols (control group, no treatment; experimental groups, treated with currents of 50 ${\mu}A$, 100 ${\mu}A$ or 300 ${\mu}A$). An experimental 20 mm linear wound was made in each animal and all animals in the experimental groups received microcurrent stimulation once a day for 20 minutes until the day of sacrifice on day 1, day 3 and day 6. An optical microscope was used to determine any histological changes. Results: The experimental results were as follows. 1. In an examination with the naked eye, all groups showed similar changes until the first day. However, from the third day, a little intercellular fluid soaked through wound region in the control group rats. In the experimental group animals, little intercellular fluid soaked through wound region, and swelling and redness did not appear, from the third day. 2. In an examination with histological evaluation, more significant changes were observed in all of the experimental group rats than the control group animals. Especially, a stimulation intensity of 50 ${\mu}A$ caused a more significant effect than the use of the other intensities by day 6 of wound healing. In addition, rapid recovery was observed. Conclusion: It was determined that microcurrent stimulation had a positive effect on wound healing. A stimulation intensity of 50 ${\mu}A$ was more effective than the other intensities (100 ${\mu}A$ and 300 ${\mu}A$) utilized for wound healing. Furthermore, low-intensity microcurrent stimulation was more effective for the purpose of wound healing.

• Annals of Clinical Neurophysiology
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• 제8권1호
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• pp.1-5
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• 2006

The head thrust maneuver is a simple bedside test of the higher frequency vestibulo-ocular reflex, which is based on Ewald's second law. It is performed by grasping the patient's head and applying a brief, small-amplitude, high-acceleration head turn, first to one side and then to the other. The patient fixates on the examiner's nose and the examiner watches for corrective rapid eye movements (saccades), which are a sign of decreased vestibular response. The "catch-up" saccades after a head thrust in one direction indicate a peripheral vestibular lesion on that side (in the labyrinth or the $8^{th}$ nerve including the root's entry zone in the brain stem). An individual pair of vertical semicircular canals can also be stimulated by turning the head to the right or left by $45^{\circ}$ and then by rotating the head in the pitch plane relative to the body. Recent studies have suggested that assessment of individual semicircular canal function by head thrust test may provide useful information for anatomical and functional details of a variety of peripheral vestibulopathies and for predicting the prognosis of vestibular neuritis. In central vestibulopathy, the head thrust test may also be valuable sign to determine dysfunction of the central pathways from individual semicircular canals and its role for the development of diverse central nystagmus.

• 생명과학회지
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• 제14권2호
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• pp.221-224
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• 2004

일반적으로 오징어의 콜레스테롤 함량은 높다고 보고하고있지만, 이는 주요 가식부인 몸통육에 대한 것이고, 여타 스테롤도 함께 정량하는 비색법에 의한 정량 결과인 경우가 많아 신뢰하기 힘들다. 그리고 이런 단점을 개선한 효소법이나 GC법은 모두 다 또 다른 단점인 고가 효소 키트가 필요하다던가 복잡한 전처리가 필요하다던가 하여 적용하기가 쉽지 않다. 따라서, 오징어 몸통 이외의 새로운 가식부로 이용되고 있는 껍질부, 내장이나 간장부위 등에는 얼마만큼의 콜레스테롤이 함유되어 있는지 정량해 보고 아울러 기존 효소법이나 GC법의 단점을 모두 극복한 Iatroscan법에 의한 콜레스테롤 정량을 시도 확립해 보았다. 또한, 이 방법의 확립을 위해 필요한 오징어 부위별 수분함량과 지방함량 및 지방종 분석도 동시에 행하였다. 한국산 물오징어 중의 부위별 수분 및 지방함량에 있어서 간장부위가 각각 50.4%, 15%로서 다른 부위와 큰 차이를 보였고, 다른 부위는 각각 75.8∼83.6%, 1.9∼4.7% 수준으로서 큰 차이는 없었다. Iatroscan법에 의한 콜레스테롤 정량을 위한 근거가 되는 지방종 조성비에 있어서는 간장부위는 92.1%가 중성지방으로서 주성분을 이루고 있었고, 몸통육과 다리육이나 껍질부위는 인지질이 81.2∼87.9%%로서 주성분이었다. 5$\alpha$-cholestane을 내부표준물질로한 Iatroscan법에 의한 콜레스테롤 정량 결과 기존의 효소법과 GC법에 의한 수치와 큰 차이를 보이지는 않아 가장 간단히 콜레스테롤을 정량해 내는 법으로 사용할 수 있다고 판단되었다. 즉, Iatroscan법으로 오징어 부위별 콜레스테롤을 정량한 결과 오징어 간장 부위에 643.2 mg%, 껍질에 608.8 mg%, 다리육에 321.4 mg%, 눈 부위에 159.5 mg%, 주가식부인 몸통육에 178.9 mg% 수준으로 콜레스테롤이 함유되어 있었다.

• 대한의용생체공학회:의공학회지
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• 제35권3호
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• pp.55-61
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• 2014

In sleep monitoring system, polysomnography (PSG) is the gold-standard but previous studies revealed that attaching numerous amount of sensors disturb sleep during the test which is the fundamental disadvantage of PSG. We suggest an unconstrained rapid-eye-movement (REM) sleep monitoring method measured with polyvinylidene (PVDF) film-based sensor for the normal and the obstructive sleep apnea (OSA) patients. Nine normal subjects and seventeen OSA patients have participated in the study. During REM sleep, rate and variability of respiration are known to be greater than in other sleep stages. Based on this phenomena, respiratory signals of participants were unconstrainedly measured using the PVDF-based sensor with the PSG and REM sleep were extracted from the average rate and variability of respiration. In epoch-by-epoch REM sleep detection, proposed method classified REM sleep with an average sensitivity of 72.3%, specificity of 92.5%, accuracy of 88.9%, and kappa statistic of 0.60 compared to the results of PSG. Student's t-test showed no significant difference between the results of normal and OSA group. This method is potentially applicable to REM sleep detection in homing environment or ambulatory monitoring.

• Biomolecules & Therapeutics
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• 제25권2호
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• pp.105-111
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• 2017

It has been known that RA, one of major constituents of Perilla frutescens which has been used as a traditional folk remedy for sedation in oriental countries, shows the anxiolytic-like and sedative effects. This study was performed to know whether RA may enhance pentobarbital-induced sleep through ${\gamma}-aminobutyric$ acid $(GABA)_A-ergic$ systems in rodents. RA (0.5, 1.0 and 2.0 mg/kg, p.o.) reduced the locomotor activity in mice. RA decreased sleep latency and increased the total sleep time in pentobarbital (42 mg/kg, i.p.)-induced sleeping mice. RA also increased sleeping time and number of falling sleep mice after treatment with sub-hypnotic pentobarbital (28 mg/kg, i.p.). In electroencephalogram (EEG) recording, RA (2.0 mg/kg) not only decreased the counts of sleep/wake cycles and REM sleep, but also increased the total and NREM sleep in rats. The power density of NREM sleep showed the increase in ${\delta}-waves$ and the decrease in ${\alpha}-waves$. On the other hand, RA (0.1, 1.0 and $10{\mu}g/ml$) increased intracellular $Cl^-$ influx in the primary cultured hypothalamic cells of rats. RA (p.o.) increased the protein expression of glutamic acid decarboxylase ($GAD_{65/67}$) and $GABA_A$ receptors subunits except ${\beta}1$ subunit. In conclusion, RA augmented pentobarbital-induced sleeping behaviors through $GABA_A-ergic$ transmission. Thus, it is suggested that RA may be useful for the treatment of insomnia.