• Journal of the Korea Institute of Military Science and Technology
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• v.27 no.1
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• pp.116-125
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• 2024

The COVID-19 pandemic is not over despite the emergency use authorization as can see recent COVID-19 daily confirmed cases. The viruses are not only difficult to diagnose and treat due to random mutations, but also pose threat human being because they have the potential to be exploited as biochemical weapons by genetic manipulation. Therefore, it is inevitable to the rapid antibody-based therapeutic platform to quickly respond to future pandemics by new/re-emerging viruses. Although numerous researches have been conducted for the fast development of antibody-based therapeutics, it is sometimes hard to respond rapidly to new viruses because of complicated expression or purification processes for antibody production. In this study, a novel rapid antibody-based therapeutic platform using single B cell sorting method and mRNA-antibody. High immunogenicity was caused to produce antibodies in vivo through mRNA-antigen inoculation. Subsequently, antigen-specific antibody candidates were selected and obtained using isolation of B cells containing antibody at the single cell level. Using the antibody-based therapeutic platform system in this study, it was confirmed that novel antigen-specific antibodies could be obtained in about 40 days, and suggested that the possibility of rapid response to new variant viruses.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.1 s.94
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• pp.95-107
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• 1999

The design models of a new product in general are created using clay models or wooden mock-ups. The reverse engineering(RE) technology enables us to quickly create the CAD model of the new product by capturing the surface of the model using laser digitizers or coordinate measuring machines. Rapid prototyping (RP) is another technology that can reduce the product development time by fabricating the physical prototype of a part using a layered manufacturing technique. In reverse engineering process, however, the digitizer generates an enormous amount of point data, and it is time consuming and also inefficient to create surfaces out of these data. In addition, the surfacing operation takes a great deal of time and skill and becomes a bottleneck. In rapid prototyping, a faceted model called STL file has been the industry standard for providing the CAD input to RP machines. It approximates the CAD model of a part using many planar triangular patches and has drawbacks. A novel procedure that overcomes these problems and integrates RE with RP is proposed. Algorithms that drastically reduce the point clouds data have been developed. These methods will facilitate the use of reverse engineered geometric data for rapid prototyping, and thereby will contribute in reducing the product development time.

• Proceedings of the Korea Concrete Institute Conference
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• 2001.11a
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• pp.1029-1034
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• 2001

The purpose of this research is to develop rapid setting cement latex modified concrete (RSLMC) which will be used to overlay bridge deck for maintaining and repairing. The main experimental variables were the types of rapid setting cement and variation of latex and antifoam agent contents were selected as admixture factor, then the properties of workability and strength development and durability properties were investigated. The results of this study show that latex content give increment of a slump due to surface tension in polymer particles and reduce unit weight of water for preservation of workability. In addition, When no and 1.6~3.2% antifoam agent were mixed, 8%, 2.0~3.8% were respectively obtained. An increasing the amount of latex produced concrete with increased flexural strength, but with slightly lower compressive strength. Rapid chloride permeability and freezing-thawing test carried out. As a results, according to increment of containing ratio antifoamer, strength of RSLMC increase, permeability showed lower value than ignorable 100 coulombs. Also, in the case of more than antifoamer 1.6%, the relativity dynamic modulus is mantained more than 90%, but in case of 0, 5%, it decrease. In consequence, with the view of strength and workability of RSLMC, it is considered that appropriate content ratio of antifoam agent and latex solid are respectively 1.6% by latex weight, 15% by cement weight.

• Clinical and Experimental Reproductive Medicine
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• v.28 no.1
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• pp.55-63
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• 2001

Objectives: This study was carried out to evaluate the effects of embryonic stage, cryoprotectant, and freezing-thawing method on the rates of survival and development of the cryopreserved mouse early embryo and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Materials and Methods: Two to eight cell embryos were obtained from oviducts of mated $F_1$ hybrid female mice superovulated by pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadotropin (hCG). Two-step 1,2-propanediol (PROH), dimethylsulfoxide (DMSO) and 4-step PROH DMSO were used as cryoprotectant and dehydration and rehydration method of embryos, and slow-cooling or rapid-cooling method was used as frozen program. The survival rates of embryos were measured after thawing and rehydration, and the developmental rates of embryos were compared and observed during culturing embryos for 24, 48, 72, 96 hrs. Results: As for the survival and development rates of embryos according to embryonic stage, the survival rate of 2 cell stage in PROH and DMSO was significantly higher than 4-8 cell (64.5% versus 62.1 %,79.7% versus 73.2%) (p<0.01, p<0.01), but the development rates of 4-8 cell embryos in PROH and DMSO were significantly higher than 2 cell embryos for whole culture period (p<0.01) and the development rates of 4-8 cell embryos in PROH were significantly higher than 2 cell embryos in DMSO (p<0.01). As for the survival and development rates of embryos according to cryoprotectant, the survival rate of 2 cell embryo in DMSO was significantly higher than that in PROH (74.4% versus 64.5%) (p<0.01), whereas the development rate of embryos was not differ till 24 hrs. The developmen1 rate from morular to hatching blastocyst, however, was significantly higher in PROH than in DMSO during 48 hr (p<0.01). The survival rate of 4-8 cell embryo was 62.1% in PROH and 73.2% in DMSO. The development rates of embryo in PROH were significantly higher for whole culture periods (p<0.01, 0.05). In respect to the effect of freezing and thawing program on the survival and development rates of embryos, method of slow cooling and rapid thawing was more effective than that of rapid cooling and rapid thawing. Conclusions: The survival rate of embryo in 2 cell stage was higher than in 4-8 cell stage, and PROH appears more effective cryoprotectant than DMSO because PROH showed better development rates of embryos in 2 and 4-8 cell stage. Moreover, slow cooling and rapid thawing method was considered as the best cryopreservation program.

• Journal of the Korean Institute of Rural Architecture
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• v.13 no.1
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• pp.63-70
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• 2011

This study is about characteristic of rural areas development project considering Rural Areas Migration. As a Rural Economy is getting worse for a rapid decrease in population and a rapid aging of the population, Decline in Rural areas has become more serious. So, Each local government is promoting rural areas development project, like Culture Village Project, Rural Village Project, Happiness Village Project, and Farming and Fishing New-town Project, to attract inhabitants in the Urban Area. This study examines the change of policy about rural areas development project in Corea through theoretical study, and analyzes the Implementation of rural village Project supported or implemented by local community to characterize the project.

• Journal of the Korean Society for Precision Engineering
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• v.17 no.10
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• pp.17-25
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• 2000

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.86-87
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• 2002

Recent technological innovations in the drug discovery process such as combinatorial synthesis and high throughput screening have led to the identification of an increasingly large number of compounds at the hits-to-leads stage. Therefore, rapid and precise pharmacokinetic/metabolic screening is essential to enhance the tractability of selected leads and to minimize the risk of failure in the later stages of drug development. (omitted)

• Computational Structural Engineering
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• v.10 no.3
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• pp.15-21
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• 1997

Modern ocean-going vessels are highly dynamically loaded all-welded structures. Traditionally, this fact has been taken into account in the design of ship structures by keeping the overall stress level within permissible limits and by limiting notch effects in the design of local structures. With an improving knowledge of loads and the rapid development of calculation means more detailed calculation-based methods are increasingly introduced. A brief review on fatigue assessment methods used in the design of ship structures has been given during the 1993 IIW International Conference in Glasgow. The rapid development during recent years underlines the importace of the subject and justifies its extended discussion in this paper.

• Journal of the Korean Society for Precision Engineering
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• v.16 no.6
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• pp.52-57
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• 1999

Funtional metal prototypes are often required in numerous industrial applications. These components are typically needed in the early stage of a project to determine form, fit and function. Recent R/P(Rapid Prototyping) part are made of soft materials such as plastics, wax, paper, these master models cannot be employed durable test in real harsh working environment. Parts by direct metal rapid tooling method, such as laser sintering, by now are hard to get net shape, pores of the green parts of powder casting method must be infiltrated to get proper strength as tool, and new type of 3D direct tooling system combining fabrication welding arc and cutting process is reported by song etc. But a system which can build directly 3D parts of high performance functional material as metal part would need long period of system development, massive investment and other serious obstacles, such as patent. In this paper, through the rapid tooling process as silicon rubber molding using R/P master model, and fabricate wax pattern in that silicon rubber mold using vacuum casting method, then we tranlsated the wax patterns to numerous metal prototypes by new investment casting process combined conventional investment casting with rapid pototyping & rapid tooling process. with this wax-injection-mold-free investment casting, we developed new investment casting process of fabricating numerous functional metal prototypes from one master model, combined 3-D CAD, R/P and conventional investment casting and tried to expect net shape measuring total dimension shrinkage from R/P part to metal part.

• Korean Journal of Computational Design and Engineering
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• v.2 no.1
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• pp.35-43
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• 1997

Concurrent Engineering is one of the methods which are used for the rapid product development. One of the important features in Concurrent Egineering is that the development process is to be parallel and the organization should be cross-functional. In order that the process be parallel and that the organization be cross-functional, an integrated information system such as PDM (Product Data Management) is required. Although the integrated data base is constructed, it could be meaningless if the application softwares were not inter-operable. This study shows an example of intergrated information system from three-dimensional product design to mold design and tooling for the development of Deflection Yoke(DY) which is one of the important parts of Cathode Ray Tube(CRT). A three-dimensional product design software, which is based on a commercial code, has been developed by ourselves. Selective Laser Sintering(SLS), which is one of the rapid prototyping techniques, has been used in this study. Mold design has been done by the three-dimensional way. A newly developed method of mold tooling, which is called Quick Die Manufacturing(QDM), has been introduced.