• Biomedical Science Letters
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• v.5 no.1
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• pp.95-100
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• 1999

It is generally difficult, time-consuming, and expensive for the clinical laboratory to detect vancomycin resistant enterococci (VRE). The aim of this study was to develop and evaluate the multiplex polymerase chain reaction (PCR) assay system as a diagnostic tool for the rapid detection of VRE from clinical samples and/or for the identification of VRE from the bacterial strains isolated from clinical specimens. Specific primers, designed from the nucleotide sequences respectively encoding the vanA, vanB, vanC-1, vanC-2/3 genes in enterococci, were coupled in a multiplex PCR assay system. With this multiplex PCR assay system, we investigated the incidence rates and types of VRE isolated from clinical samples. A total of 75 strains of enterococci were isolated in 3 general hospitals in Korea. Of these isolates, 36 strains showed a pattern of high-level vancomycin resistance which associated with vanA gene, whereas 18 strains showed low-level vancomycin resistance associated with vanC-1 or vanC-2/3 gene. Thus, multiplex PCR assay method established in this study could be applied for the rapid detection of VRE.

• The Journal of the Korea institute of electronic communication sciences
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• v.18 no.2
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• pp.355-360
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• 2023

Fire is a disaster that causes irreversible damage to many people due to personal injury and property damage. Various fire detection equipments are installed around us to detect and cope with it quickly. However, due to various problems such as artificial, environmental, and aging, fire detection equipment is activated even though it is not a actual fire, and there are many problems such as delaying the support to the necessary fire scene. In this paper, we analyze the non-fire alarm of the fire detection equipment and propose a system that enables the field staff to check the scene situation through the video as a way to prevent the mobilization due to the misinformation by checking the fire. The purpose of the present invention is to stably cope with a disaster by suggesting a customized automatic guidance system which induces a rapid evacuation by sending an evacuation guidance notification to a range of a fire occurrence neighboring area, and supports a rapid and accurate processing by a rapid dispatch of a firefighter, rather than a wide range of guidance such as an existing emergency disaster guidance letter when it is determined to be an actual fire through the confirmation procedure.

• Journal of the Institute of Convergence Signal Processing
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• v.2 no.1
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• pp.12-18
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• 2001

SAR radar and FLIR images, which are taken from sensors on aircrafts or satellites, are compressed prior to transmission to facilitate rapid transfer through the limited bandwidth channels. In this case, it is important that it achieves compression ratio as high as possible as well as high target detection rate. In this paper a joint ATR-compression system based on the subband coding and VQ is proposed, which utilizes the encoder as a predictor or classifier for target detection. Simulation result shows that the proposed system achieves a relatively high level of target detection performance as well as a high compression ratio over 200:1.

• The Transactions of the Korea Information Processing Society
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• v.6 no.5
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• pp.1332-1341
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• 1999

Rapid expansion of network and increment of computer system access cause computer security to be an important issue. Hence, the researches in intrusion detection system(IDS)are active to reduce the risk from hackers. Considering IDS, we propose a new IDS model(DABIDS : Distributed Agent Based Intelligent intrusion Detection System) based on distributed intrusion detecting agents. The DABIDS dynamically collects intrusion behavior knowledge from each agents when some doubtable behaviors of users are detected and make new agents codes using intrusion scenario data base, and broadcast the detector codes to the distributed intrusion detecting agent of all node. This DABIDS can efficiently solve the problem to reduce the overhead for training detecting agent for intrusion behavior patterns.

• Journal of Dairy Science and Biotechnology
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• v.33 no.4
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• pp.271-275
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• 2015

Colloidal semiconductor CdSe-ZnS core-shell nanocrystal quantum dot (Qdot) are luminescent inorganic fluorophores that show potential to overcome some of the functional limitations encountered with organic dyes in fluorescence labeling applications. Salmonella Enteritidis has emerged as a major cause of human salmonellosis worldwide since the 1980s. A rapid, specific, and sensitive method for the detection of Salmonella Enteritidis was developed using Qdot as a fluorescence marker coupled with immunomagnetic separation. Magnetic beads coated with anti-Salmonella Enteritidis antibodies were employed to selectively capture the target bacteria, and biotin-conjugated anti-Salmonella antibodies were added to form sandwich immune complexes. After magnetic separation, the immune complexes were labeled with Qdot via biotin-streptavidin conjugation, and fluorescence measurement was carried out using a fluorescence measurement system. The detection limit of the Qdot method was a Salmonella Enteritidis concentration of $10^3$ colony-forming units (CFU)/mL, whereas the conventional fluorescein isothiocyanate-based method required over $10^5CFU/mL$. The total detection time was within 2 h. In addition to the potential for general nanotechnology development, these results suggest a new rapid detection method of various pathogenic bacteria from a complex food matrix.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.23 no.12
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• pp.1535-1541
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• 2019

To carry out safe and rapid decontamination in radiological accident areas, acquisition of various information on radiation sources is needed. In particular, to figure out the location and distribution of radiation sources is essential for rapid follow-up and removal of contaminants as well as minimizing worker damage. The radiation distribution detection device is used to obtain the position and distribution information of the radiation source. In the case of a radiation distribution detection device, a detection sensor unit is generally composed of a single sensor, and the detection range is limited due to the physical characteristics of the single sensor. We applied a calibration detector for controlling the detection sensitivity of a single sensor for radiation detection and improved the limited detection range of radiation dose rate. Also, gamma irradiation test confirmed the improvement of radiation distribution detection range.

• Journal of Dairy Science and Biotechnology
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• v.29 no.2
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• pp.51-57
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• 2011

Milk and dairy products are nutritionally one of the most important food in human health and the quality of raw milk is significantly important to ensure safety of dairy products. However, milk and dairy products are commonly related with chemical and microbial contaminations. Therefore, rapid and reliable detection of hazardous (e.g. pathogenic bacteria, pesticides, antibiotics, microbial toxins) in milk and dairy products is essential to ensure human health and food safety. Conventional methods for detection of food hazardous are mostly time-consuming to yield a results. Recently, biosensors have been focused as its rapidity and high sensitivity to analyse chemical and microbial hazardous from a variety of foods and environments. This study reviewed the recent trends and applications of biosensors as rapid detection method of hazardous in milk and dairy products.

• The KIPS Transactions:PartC
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• v.16C no.1
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• pp.13-20
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• 2009

Recently, as traffic flooding attacks such as DoS/DDoS and Internet Worm have posed devastating threats to network services, rapid detection and proper response mechanisms are the major concern for secure and reliable network services. However, most of the current Intrusion Detection Systems (IDSs) focus on detail analysis of packet data, which results in late detection and a high system burden to cope with high-speed network traffic. In this paper we propose an SNMP-based lightweight and fast detection algorithm for traffic flooding attacks, which minimizes the processing and network overhead of the detection system, minimizes the detection time, and provides high detection rate. The attack detection algorithm consists of three consecutive stages. The first stage determines the detection timing using the update interval of SNMP MIB. The second stage analyzes attack symptoms based on correlations of MIB data. The third stage determines whether an attack occurs or not and figure out the attack type in case of attack.

• Journal of Multimedia Information System
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• v.6 no.1
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• pp.1-6
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• 2019

In the field of industrial applications, the real-time performance of the target detection problem is very important. The most serious time consumption in the pedestrian detection process is the extraction phase of the candidate window. To accelerate the speed, in this paper, a fast extraction of pedestrian candidate window based on the BING (Binarized Normed Gradients) algorithm replaces the traditional sliding window scanning. The BING features are extracted with the positive and negative samples and input into the two-stage SVM (Support Vector Machine) classifier for training. The obtained BING template may include a pedestrian candidate window. The trained template is loaded during detection, and the extracted candidate windows are input into the classifier. The experimental results show that the proposed method can extract fewer candidate window and has a higher recall rate with more rapid speed than the traditional sliding window detection method, so the method improves the detection speed while maintaining the detection accuracy. In addition, the real-time requirement is satisfied.

• The Plant Pathology Journal
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• v.40 no.1
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• pp.40-47
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• 2024

Garlic can be infected by a variety of viruses, but mixed infections with leek yellow stripe virus, onion yellow dwarf virus, and allexiviruses are the most damaging, so an easy, inexpensive on-site method to simultaneously detect at least these three viruses with a certain degree of accuracy is needed to produce virus-free plants. The most common laboratory method for diagnosis is multiplex reverse transcription polymerase chain reaction (RT-PCR). However, allexiviruses are highly diverse even within the same species, making it difficult to design universal PCR primers for all garlic-growing regions in the world. To solve this problem, we developed an inexpensive on-site detection system for the three garlic viruses that uses a commercial mobile PCR device and a compact electrophoresis system with a blue light. In this system, virus-specific bands generated by electrophoresis can be identified by eye in real time because the PCR products are labeled with a fluorescent dye, FITC. Because the electrophoresis step might eventually be replaced with a lateral flow assay (LFA), we also demonstrated that a uniplex LFA can be used for virus detection; however, multiplexing and a significant cost reduction are needed before it can be used for on-site detection.