• Journal of the Korean Applied Science and Technology
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• v.35 no.4
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• pp.1269-1275
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• 2018

Raphiolepis indica (R. indica) is one of evergreen shrubs belonging to the Rosaceae and is grown wildly in Jeju. This study was performed to evaluate the hepatoprotective effect of different fractions (n-hexane, dichloromethane, ethyl acetate, butanol, water) from R. indica. Anti-oxidative effects were determined using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activity and total phenol contents. Hepatoprotective effect was identified by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) assay in Huh7 cells. Among various fractions, ethyl acetate and butanol fractions showed the lowest DPPH remained rate reaching approximately 78.7 and 65.5% at $400{\mu}g/mL$. Ethyl acetate and butanol fractions showed the total phenolic content at 164.5 and 137.3 mg GAE/g extract. The ethyl acetate and butanol fractions were resistant against oxidative stress in MTT assay and showed higher hepatoprotective effect than other fractions. Therefore, these results suggest that the ethyl acetate and butanol fractions of R. indica might have therapeutic value in liver damage.

• Horticultural Science & Technology
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• v.31 no.5
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• pp.617-625
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• 2013

Stauntonia hexaphylla and Raphiolepis indica, cold-tolerant broadleaved evergreens ranging through the southern region of South Korea, were assessed on salt tolerance with NaCl treatment using visual damage, chlorophyll florescence image, and malondialdehyde (MDA) analysis. As NaCl concentrations increased, the soil pH decreased and EC increased, and the soil of S. hexaphylla was affected more strongly by the treatment than that of R. indica. In visual damage, S. hexaphylla withered above 200 mM NaCl at 20 days after the treatment. All individuals of R. indica survived during the experiment though the leaves of R. indica showed visual damages up to 400 mM NaCl. The color changes in chlorophyll fluorescence showed a strong correlation with the degree of visual damage. As NaCl increased, the red color of the leaves of S. hexaphylla was distinctly changed to blue and chlorophyll fluorescence decreased starting from the margin to the middle of a leaf. R. indica showed subtle color changes and remained in red color during the experiment. At five days after the NaCl treatment, the MDA of S. hexaphylla was above $4.56nmol{\cdot}g^{-1}$ when plants showed the highest visual damage and EC. The MDA of R. indica in all treatments showed below $1.5nmol{\cdot}g^{-1}$ except 400 mM NaCl treatment during the experiment.

• Journal of Environmental Science International
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• v.23 no.3
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• pp.335-357
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• 2014

This study aims at classifying and interpreting on the vegetation structure and the vegetation transition over 25 years (between 1986 and 2010), and the correlation with the change of some conditions (the vegetation height and coverage on each layer and the climate factors as WI, CI, mean annual temperature, mean annual total precipitation etc.) in the Evergreen Broad-Leaved Forest,, Hong-Do island. The EBLF is classified into five units of vegetation (Hedera rhombea-Machilus thunbergii community (M-M comm.), Castanopsis sieboldii forest (Machilus japonica-Castanopsis sieboldii community; Raphiolepis indica var. umbellata-C. sieboldii community), community (Qa comm.), Carpinus turczaninovii community (Ct comm.), Camellia japonica stand (Cj stand)). The vegetation transition by CCA had high correlation with the height and coverage on each layer and the climate factors, and it did the succession (transition) that the M-M comm. (2010) from Mallotus japonicus community Machilus thunbergii community Carpinus coreana community (Cc comm.) Aucuba japonica community (Aj comm.) Trachelos permum asiaticum var. intermedium-Quercus acuta community (TQ comm.) (1986), the communities of C. sieboldii forest (2010) from Aj comm. TQ comm. Raphiolepis umbellata-Camellia japonica community (RC comm.) (1986), the Qa comm. (2010) from Ardisia japonica-Castanopsis sieboldii community (AC comm.) and TQ comm. (1986), the Ct comm. (2010) from Cc comm. RC comm. Aj comm. Quercus serrata community and the Cj stand (2010) from AC comm. (1986). the height and coverage on each layer are also changed.

• Journal of Life Science
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• v.30 no.8
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• pp.688-694
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• 2020

In a previous study, the total phenolic content in ethanol extracts of medicinal plants that naturally grow on Jeju Island were analyzed with the extracts of Raphiolepsis indica leaf found to have the highest. The current study was carried out to evaluate the total flavonoid content, radical-scavenging activity, and the protective effect of R. indica extracts and solvent fractions on oxidative stress in HaCaT keratinocytes. More specifically, total flavonoid content and 2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging activity were measured to assess anti-oxidative activity, and protective effects against hydrogen peroxide (H₂O₂) were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay on the HaCaT cells. Of the various fractions analyzed, the ethyl acetate extract of R. indica showed the highest total flavonoid content (149.13 mg/g extracts) and the lowest remaining ABTS. In addition, the ethyl acetate fraction was significantly more resistant against H₂O₂ than the negative control. Our results therefore suggest that an ethyl acetate fraction of R. indica protects HaCaT cells against oxidative stress and could prove useful for developing functional cosmetic materials.

• Journal of the Korean Applied Science and Technology
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• v.35 no.2
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• pp.492-501
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• 2018

Obesity is a worldwide disease and one of the major risk factors. Virus among many factors can lead to obesity. Adenovirus 36 (Ad-36) is the adipogenic virus linked with human obesity. Nevertheless, there is no drug to treat both Ad-36 infection and obesity associated with virus. For the precedent study on anti-cholesterol test, Distylium racemosum (D. racemosum), Quercus salicina (Q. salicina) and Raphiolepis indica (R. indica) were selected. This study was carried out to evaluate the anti-cholesterol effects, anti-lipid effects and inhibition of Ad-36 replication from three extracts. D. racemosum ($50{\mu}g/mL$) inhibited lipid accumulation on 3T3-L1 adipocyte. D. racemosum inhibited adipocyte differentiation through suppression of regulator peroxisome proliferator-activated receptor-${\gamma}$ ($PPAR{\gamma}$) genes and adipocyte-specific genes such as adipocyte protein 2 (aP2). D. racemosum inhibited replication of Ad-36 at $50{\mu}g/mL$ of concentration. Therefore, the extract of D. racemosum could be a candidate for development of anti-Ad-36 and anti-obesity drugs.

• Journal of Korean Society of Forest Science
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• v.106 no.4
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• pp.397-407
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• 2017

This research aims to analyze and compare the drought resistance of 7 species of landscape trees commonly grown in Korea. The 7 species are: Camellia japonica, Rhaphiolepis indica, Quercus glauca, Machilus thunbergii, Daphniphyllum macropodum, Dendropanax morbifera and Cinnamomum camphora. In order to analyze their drought resistance, the samples were left without irrigation for 30 days (05/09/2016 ~ 05/10/2016), during which period their respective drought resistor, relative water content, electrolyte elution figures and proline content were measured. As the non-irrigation proceeded, C. camphora was the first to wither, followed by D. morbifera, then D. macropodum, then M. thunbergii, then Q. glauca, then R. indica then finally C. japonica. Of the 7 species, Q. glauca, C. japonica and R. indica can be considered highly drought resistant, since they survived for longer than 3 weeks without irrigation. Relative water content (RWC) plummeted dramatically after the first 15 days of non-irrigation. Whereas RWC readings of C. camphora, D. morbifera, D. macropodum and M. tunbergii dropped by 40% or more, the other 4 species reported a relatively low rate of decrease at 20% or lower. The Camellia japonica, the R. indica and Q. glauca, which were the species with relatively high drought resistance, showed low proline content and electrolyte elution figures, whereas those of C. camphora, D. macropodum, D. morbifera and M. tunbergii were higher. Analysis through the nonlinear regression analysis logistic model showed that non-irrigation proved fatal for the 7 sample species in a range of 22.7 to 37.6 days. The C. japonica, R. indica, Q. glauca and M. tunbergii demonstrated a high drought resistance of 30 days or longer, whereas C. camphora, D. morbifera and D. macropodum had a low resistance of 25 days or less to drought from lack of water. In conclusion, out of the 7 species of broad-leaved evergreen trees tested, C. japonica, R. indica and Q. glauca seem to be suitable for use as landscape trees, owing to their high drought resistance.

• Journal of Korean Society of Forest Science
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• v.108 no.4
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• pp.484-492
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• 2019

The aim of the present study was to compare the cold tolerance of seven different types of trees growing in southern Korea to select evergreen broad-leaved trees that can be used as street trees in large land areas experiencing climate change. The trees compared were the thorn tree, Cinnamomum camphora, Camellia japonica, Machilus thunbergii, Dendropanax morbifera, Daphniphyllum macropodum Miq., Quercus glauca Thunb., and Raphiolepis indica. When the trees were subjected to low temperature treatment, their electrolyte elution volume values appeared to increase with the decreases in the treatment temperature. The analysis of the cold tolerance of each type of tree was based on the estimated temperatures in the following order: C. japonica (-11.586℃) > R. indica (-9.348℃) > Q. glauca (-8.719℃) > M. thunbergii (-8.090℃) > D. macropodum (-7.409℃) > D. morbifera (-7.085℃) > C. camphora (-6.995℃). The relative cold tolerance difference found in the seven tree species was more than 5℃, as evaluated previously. In the Lauraceae family, the difference in cold tolerance was more than 2℃, even in the same species. The analysis showed that trees with excellent cold tolerance included Q. glauca Thunb., C. japonica, R. indica, and the thorn tree. This knowledge is required for the evaluation of the possibility of the survival of trees under cold temperature conditions in cities.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.14 no.6
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• pp.29-39
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• 2011

This study aimed to select evergreen broad-leaved trees (EBT) that can be produced and planted in Korea by analyzing the current use of the trees for landscaping in the Kyushu area of Japan, a warm temperate region. The results revealed the total production of EBT was higher than that of conifers and due to the suitable growth environment of the area and the subsequent high demand for them. The landscape tree production methods in Japan were divided into container nursery and outdoor nursery, and the uses and species of the trees varied depending on the method; a variety of native species were produced in container nursery rather than in outdoor nursery, whereas trees used as ornamental, gardening, and shade plants were produced in outdoor nursery rather than in container nursery. The results also showed that in Fukuoka, a major city in Kyushu that is adjacent to Korea, the planting rate of EBT, used as ornamental, gardening, and shade plants, was higher than that of deciduous broad-leaved trees and conifers. In the city, the planting rate of Cinnamomum camphora was the highest, Elaeocarpus sylvestris var. ellipticus, Ternstroemia gymnanthera, Ilex rotunda, Quercus glauca, Camellia sasanqua, Eurya emarginata, Pittosporum tobira, Raphiolepis indica var. umbellata, Hedera rhombea, Kadsura japonica, Trachelospermum asiaticum var. asiaticum. These species were verified in Kyushu area for their application as landscape trees and are expected to serve as landscape trees in Korea if the planting areas of them are expanded by global warming, urban heat island and regional microclimate.