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http://dx.doi.org/10.5352/JLS.2020.30.8.688

The Protective Effects of an Ethyl Acetate Fraction of Raphiolepsis indica Against Oxidative Stress in HaCaT Keratinocytes  

Yang, Eun Ju (Department of Clinical Laboratory Science, College of Medical Sciences, Daegu Haany University)
Kim, Hye-Ran (Department of Clinical Laboratory Science, College of Medical Sciences, Daegu Haany University)
Chang, Kyung-Soo (Department of Clinical Laboratory Science, College of Health Sciences, Catholic University of Pusan)
Chang, Jeong Hyun (Department of Clinical Laboratory Science, College of Medical Sciences, Daegu Haany University)
Publication Information
Journal of Life Science / v.30, no.8, 2020 , pp. 688-694 More about this Journal
Abstract
In a previous study, the total phenolic content in ethanol extracts of medicinal plants that naturally grow on Jeju Island were analyzed with the extracts of Raphiolepsis indica leaf found to have the highest. The current study was carried out to evaluate the total flavonoid content, radical-scavenging activity, and the protective effect of R. indica extracts and solvent fractions on oxidative stress in HaCaT keratinocytes. More specifically, total flavonoid content and 2-azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) radical-scavenging activity were measured to assess anti-oxidative activity, and protective effects against hydrogen peroxide (H2O2) were determined by 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide assay on the HaCaT cells. Of the various fractions analyzed, the ethyl acetate extract of R. indica showed the highest total flavonoid content (149.13 mg/g extracts) and the lowest remaining ABTS. In addition, the ethyl acetate fraction was significantly more resistant against H2O2 than the negative control. Our results therefore suggest that an ethyl acetate fraction of R. indica protects HaCaT cells against oxidative stress and could prove useful for developing functional cosmetic materials.
Keywords
Anti-oxidants; ethyl acetate fraction; keratinocytes; oxidative stress; Raphiolepis indica;
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