• Title/Summary/Keyword: Range of motions

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DNA Dynamics: a Fluorescence Resonance Energy Transfer Study Using a Long-Lifetime Metal-Ligand Complex

  • Kang, Jung-Sook;Lakowicz, Joseph-R.;Piszczek, Grzegorz
    • Archives of Pharmacal Research
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    • v.25 no.2
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    • pp.143-150
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    • 2002
  • Fluorescent probes bound to DNA typically display nanosecond decay times and reveal only nanosecond motions. We extend the time range of measurable DNA dynamics using $[Ru(pby)_2(dppz)]^{2+}$ (bpy=2.2'-bipyridine, dppz=dipyrido[3,2-a2',3'-c]phenazine) (RuBD) which displays a mean lifetime near 90 ns. To test the usefulness of RuBD as a probe for diffusive processes in calf thymus DNA, we compared the efficiencies of fluorescence resonance energy transfer (FRET) using three donors which display lifetimes near 5 ns for acridine orange (AO), 22 ns for ethidum bromide (EB) and 92 ns for RuBD, with nile blue (NB) as the acceptor. The F rster distances for AO-NB, EB-NB and RuBD-NB donor-acceptor pairs were 42.3, 52.3, and $30.6{\;}{\AA}$, respectively. All three donors showed dramatic decreases in fluorescence intensities and more rapid intensity decays with increasing NB concentrations. The intensity decays of AO and EB in the presence of varying concentrations of NB were satisfactorily described by the one-dimensional FRET model without diffusion (Blumen and Manz, 1979). In the case of the long-lifetime donor RuBD, the experimental phase and modulation somewhat deviated from the recovered values computed from this model. The recovered NB concentrations and FRET efficiencies from the model were slightly larger than the expected values, however, the recovered and expected values did not show a significant difference. Thus, it is suggested that the lifetime of RuBD is too short to measure diffusive processes in calf thymus DNA.

Aeroelastic Behaviors of Self-anchored Suspension Bridge with Lateral Sag of Main Cable(II) - Focused on the Behavior of Tower - (횡방향 새그를 가진 자정식 현수교의 공탄성 거동(II) - 주탑의 거동을 중심으로 -)

  • Kwon, Soon Duck;Chang, Sung Pil
    • Journal of Korean Society of Steel Construction
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    • v.9 no.2 s.31
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    • pp.269-275
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    • 1997
  • Wind tunnel test results and their interpretations, which were performed to study the aerodynamic stability of tower of self-anchored suspension bridge, are presented in this paper. Tower and full models were tested under smooth and turbulent flow conditions. In the case of the tower with inclined two columns, the vibration due to wakes were occurred at wide velocity zone because the wakes with various frequencies were generated by inclined upstream column. It has to be emphasized that the vibration characteristics of the tower in the self-anchored suspension bridge may be very sensitive to the longitudinal boundary conditions of the girder at the supports. Because of the two natural frequency of the tower, out-of-plane bending and torsional, were not well separated, coupled motions were observed in a wide range of wind velocity. The effectiveness of corner cut, countermeasure to reduce the tower vibrations, was also studied. It has been found that 1:10, comer cut size to column width, may be the most effective ratio for reducing the vibrations.

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Analysis of added resistance and seakeeping responses in head sea conditions for low-speed full ships using URANS approach

  • Kim, Yoo-Chul;Kim, Kwang-Soo;Kim, Jin;Kim, Yoonsik;Park, Il-Ryong;Jang, Young-Hun
    • International Journal of Naval Architecture and Ocean Engineering
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    • v.9 no.6
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    • pp.641-654
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    • 2017
  • The KVLCC2 and its modified hull form were investigated in regular head waves using Unsteady Reynolds Averaged Navier-Stokes (URANS) methods. The modified KVLCC2 (named KWP-bow KVLCC2) is designed for reducing wave reflection from the bow. Firstly, the original KVLCC2 is studied for verification of the present code and methodology and the computed time history of total resistance and 2DOF motions (heave and pitch) for the selected two wave length conditions are directly compared with the results obtained from KRISO towing tank experiment under the identical condition. The predicted added resistance, heave and pitch motion RAOs show relatively good agreement with the experimental results. Secondly, the comparison of performance in waves between KVLCC2 and KWP-bow KVLCC2 is carried out. We confirmed that newly designed hull form shows better performances in all the range of wave length conditions through both the computation and the experiment. The present URANS method can capture the difference of performance in waves of the two hull forms without any special treatment for short wave length conditions. It can be identified that KWP-bow KVLCC2 gives about 8% of energy saving in sea state 5 condition.

Development of Gesture Recognition-Based 3D Serious Games (치매 예방을 위한 제스처 인식 기반 3D 기능성 게임 개발)

  • He, Guan-Feng;Park, Jin-Woong;Kang, Sun-Kyung;Jung, Sung-Tae
    • Journal of Korea Game Society
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    • v.11 no.6
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    • pp.103-113
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    • 2011
  • In this paper, we propose gesture recognition based 3D Serious Games to prevent dementia. These games are designed to enhance the effect of preventing dementia by helping increase brain usage and physical activities of users by the entire body gesture recognition. The existing cameras used for gesture recognition technology are limited in terms of recognition ratio and operation range. For more stable recognition of the body gestures, we recognized users with a 3D depth camera, obtained joint data of users, and analyzed joint motions to recognize gestures of the body. Game contents were designed to practice memory, reasoning, calculation, and spatial recognition focusing on the atrophy of brain cells as a major cause of dementia. Game results of each user were saved and analyzed to measure how their recognition skills improved.

A Study on the Wearing Conditions for Developing Police Raincoat with Advanced Work Efficiency (업무효율성이 향상된 경찰 우의 개발을 위한 경찰 우의 착의실태 조사)

  • Yu, Seung-min;Kim, Dong-Eun
    • Journal of the Korean Society of Clothing and Textiles
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    • v.40 no.5
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    • pp.910-920
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    • 2016
  • This study is to understand the problems of current police raincoats for professional policemen and conscripted policemen. Interviews and a survey were completed to investigate the wearing conditions of current police raincoats. The questionnaire was composed of questions on current wearing conditions of raincoat, motional adaptability, dimensional compatibility, design suitability and demographic information on the research target. The results indicated that the wearing frequency of raincoats had significant difference according to duty and age range. The main reason for the low wearing frequency was the uncomfortableness of working while wearing the raincoat, followed by the inconvenience of managing the raincoat after use and feeling hot while wearing the raincoat. The three most important factors when wearing a raincoat was pleasantness, activity capability and the functionality of the fabric. The satisfaction on body parts during motions tended to be evaluated lower as the raincoat size increased. The results of the dissatisfaction factors indicated that the raincoat hood had the most problems. There was inconvenience when using the equipment belt because officers have to wear their belts inside the raincoat. In regards to the fabric satisfaction level, respondents were less satisfied with ventilation, hygroscopicity, and drying time. Therefor it appeared that the current raincoat had problems due a lack of functionality in regards to ventilation, hygroscopicity, and quick drying; in addition, the satisfaction on motional adaptability had a significant difference according to raincoat size.

A Biomechanical Comparison among Three Surgical Methods in Bilateral Subaxial Cervical Facet Dislocation

  • Byun, Jae-Sung;Kim, Sung-Min;Choi, Sun-Kil;Lim, T. Jesse;Kim, Daniel H.
    • Journal of Korean Neurosurgical Society
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    • v.37 no.2
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    • pp.89-95
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    • 2005
  • Objective: The biomechanical stabilities between the anterior plate fixation after anterior discectomy and fusion (ACDFP) and the posterior transpedicular fixation after ACDF(ACDFTP) have not been compared using human cadaver in bilateral cervical facet dislocation. The purpose of this study is to compare the stability of ACDFP, a posterior wiring procedure after ACDFP(ACDFPW), and ACDFTP for treatment of bilateral cervical facet dislocation. Methods: Ten human spines (C3-T1) were tested in the following sequence: the intact state, after ACDFP(Group 1), ACDFPW(Group 2), and ACDFTP(Group 3). Intervertebral motions were measured by a video-based motion capture system. The range of motion(ROM) and neutral zone(NZ) were compared for each loading mode to a maximum of 2.0Nm. Results: ROMs for Group 1 were below that of the intact spine in all loading modes, with statistical significance in flexion and extension, but NZs were decreased in flexion and extension and slightly increased in bending and axial rotation without significances. Group 2 produced additional stability in axial rotation of ROM and in flexion of NZ than Group 1 with significance. Group 3 provided better stability than Group 1 in bending and axial rotation, and better stability than Group 2 in bending of both ROM and NZ. There was no significant difference in extension modes for the three Groups. Conclusion: ACDFTP(Group 3) demonstrates the most effective stabilization followed by ACDFPW(Group 2), and ACDFP(Group 1). ACDFP provides sufficient strength in most loading modes, ACDFP can provide an effective stabilization for bilateral cervical facet dislocation with a brace.

An Analysis of Kinematics and EMG for Bandal Chagi in Taekwondo (태권도 반달차기 시 운동학적 변인 및 근전도 분석)

  • Ha, Chul-Soo;Choi, Man-Ho
    • Korean Journal of Applied Biomechanics
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    • v.18 no.3
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    • pp.11-21
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    • 2008
  • The purpose of this study was to investigate the most optimum way of performing the bandal chagi during Taekwondo Kyorugi competition. By analyzing the EMG data and the kinematic data it was hoped that scientific data would be provided to instructors and players about the optimization of the Bandal Chagi. The results of the analysis are as follows: During competition while performing the Bandal Chagi the most important factors that affect the impact point are the range of motions of the upper body hyper extension and knee joints. Through the measurement of the muscles EMG activity of a well performed Bandal Chagi with the right leg it was observed that the left side erector spinae muscle was highly activated and so it was concluded that this muscle should be trained to improve the performance of the Bandal Chagi. Likewise it was observed that for the right side of the abdominal muscle's EMG there was a high activity level and thus showed that there was a large contribution of this muscle for the optimum performance of the Bandal Chagi.

Micro Vibration Measurement in a Latex Sample Mimicking the Tympanic Membrane Using Micro Vibro Tomography (고막을 모방한 라텍스 샘플의 미세진동 측정을 위한 마이크로 바이브로 토모그라피 시스템 개발)

  • Kwon, Jaehwan;Kim, Pilun;Jeon, Mansik;Kim, Jeehyun
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.30 no.1
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    • pp.23-27
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    • 2019
  • In this paper, we propose a micro vibro tomography(MVT) method, that can be used to visualize two-dimensional cross-sectional images and micro-vibration tomographic images in real time in a non-contact and non-destructive manner. The proposed method is based on the optical coherence tomography(OCT) technique, with an additionally customized image processing algorithm. The proposed method can detect the micro-motions or vibrations in sample structures by measuring the phase shift variations in the sample structures. In this study, we show the potential capabilities of the proposed MVT system for measuring the micro-vibrations generated when sound waves in a frequency range of 2~5 kHz are applied to an $80-{\mu}m$ thick latex phantom, which mimics the changes in physical structure of the human tympanic membrane while hearing. Additionally, three-dimensional volumetric images of the MVT method were recorded to observe the surface morphological changes in the surface of the phantom sample which mimics the human tympanic membrane while hearing.

The Controller Design for Lane Following with 3-Degree of Freedom Vehicle Dynamics (3자유도 차량모델을 이용한 차선추종 µ 제어기 설계)

  • Ji, Sang-Won;Lim, Tae-Woo;You, Sam-Sang;Kim, Hwan-Seong
    • Journal of Power System Engineering
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    • v.17 no.3
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    • pp.72-81
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    • 2013
  • Many articles have been published about a 2-degree of freedom model that includes the lateral and yaw motions for controller synthesis in intelligent transport system applications. In this paper, a 3-degree of freedom linear model that includes the roll motion is developed to design a robust steering controller for lane following maneuvers using ${\mu}$-synthesis. This linear perturbed system includes a set of parametric uncertainties in cornering stiffness and unmodelled dynamics in steering actuators. The state-space model with parametric uncertainties is represented in linear fractional transformation form. Design purpose can be obtained by properly choosing the frequency dependent weighting functions. The objective of this study is to keep the tracking error and steering input energy small in the presence of variations of the cornering stiffness coefficients. Furthermore, good ride quality has to be achieved against these uncertainties. Frequency-domain analyses and time-domain numerical simulations are carried out in order to evaluate these performance specifications of a given vehicle system. Finally, the simulation results indicate that the proposed robust controller achieves good performance over a wide range of uncertainty for the given maneuvers.

Development of Active Seat Suspension with 2 DOF for Agricultural Tractors(I) - Development of Control System for Active Seat Suspension - (농용트랙터를 위한 2자유도를 갖는 능동형 좌석 현가장치 개발(I) - 능동형 좌석 현가장치 제어시스템의 개발 -)

  • Yu, Ji-Hoon;Lee, Kyu-Cheol;Kim, Ki-Young;Park, Hyung-Bae;Ryu, Kwan-Hee
    • Journal of Biosystems Engineering
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    • v.34 no.5
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    • pp.315-324
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    • 2009
  • Various types of vibration are transmitted to operators of agricultural tractors while working in the field. Most harmful vibration to human body is ride vibrations with low frequency ranging from 1 to 10 Hz, caused by rough terrain. These ride vibration has vertical and rotational components. This study was conducted to develop an active seat suspension system with two degrees of freedoms, enabling effectively reduce vibrations in vertical and pitch motions. Therefore, a mechanism for the active seat suspension was developed, and an electro-hydraulic servo system and a controller to drive the active seat suspension system were also developed in this study. A simulation model was developed to evaluate how the active seat suspension system effectively reduce the vibrations transmitted to the base of seat. Active seat suspension was optimized to enhance the performance using the developed simulation model. The performance of the seat suspension system was evaluated according to the test codes described in EEC78/764 in order to investigate the feasibility of application to agricultural tractors. The result showed that the developed active seat suspension system could reduce the magnitude of vertical vibration up to 80% for the input vibrations according to the test codes described in EEC78/764. The system could reduce the rotational displacement of ${\pm}\;2.5$ degrees up to 50% for the pitch vibration on the average in the frequency range of 1 to 2 Hz.