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The effect of resin cement type and cleaning method on the shear bond strength of resin cements for recementing restorations

  • Koodaryan, Roodabeh;Hafezeqoran, Ali;Maleki, Amin Khakpour
    • The Journal of Advanced Prosthodontics
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    • v.9 no.2
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    • pp.110-117
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    • 2017
  • PURPOSE. This laboratory study assessed the effect of different dentin cleaning procedures on shear bond strength of resin cements for recementing prosthesis. MATERIALS AND METHODS. A $4{\times}4$ flat surface was prepared on the labial surface of 52 maxillary central incisors. Metal frames ($4{\times}4{\times}1.5mm$) were cast with nickel-chromium alloy. All specimens were randomly divided into 2 groups to be cemented with either Panavia F2.0 (P) or RelyX Ultimate (U) cement. The initial shear bond strength was recorded by Universal Testing Machine at a crosshead speed of 0.5 mm/min. Debonded specimens were randomly allocated into 2 subgroups (n = 13) according to the dentin cleaning procedures for recementation. The residual cement on bonded dentin surfaces was eliminated with either pumice slurry (p) or tungsten carbide bur (c). The restorations were rebonded with the same cement and were subjected to shear test. Data failed the normality test (P < .05), thus were analyzed with Mann Whitney U-test, Wilcoxon signed rank test, and two-way ANOVA after logarithmic transformation (${\alpha}=.05$). RESULTS. The initial shear bond strength of group P was significantly higher than group U (P = .001). Pc and Uc groups presented higher bond strength after recementation compared to the initial bond strength. However, it was significant only in Pc group (P = .034). CONCLUSION. The specimens recemented with Panavia F2.0 provided higher bond strength than RelyX Ultimate cement. Moreover, a tungsten carbide bur was a more efficient method in removing the residual resin cement and increased the bond strength of Panavia F2.0 cement after recementation.

Development of Numerical Technique to Analyze the Flow Characteristics of Porous Media Using Lattice Boltzmann Method (격자볼쯔만법을 이용한 다공체의 유동특성 분석방법 개발에 관한 연구)

  • Kim, Hyung Min
    • Transactions of the Korean Society of Mechanical Engineers B
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    • v.40 no.11
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    • pp.689-695
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    • 2016
  • The performance of proton exchange membrane fuel cells (PEMFC) is strongly related to the water flow and accumulation in the gas diffusion layer (GDL) and catalyst layer. Understanding the behavior of fluid from the characteristics of the media is crucial for the improvement of the performance and design of the GDL. In this paper, a numerical method is proposed to calculate the design parameters of the GDL, i.e., permeability, tortuosity, and effective diffusivity. The fluid flow in a channel filled with randomly packed hard spheres is simulated to validate the method. The flow simulation was performed by lattice Boltzmann method with bounce back condition for the solid volume fraction in the porous media, with different values of porosities. Permeability, which affects the flow, was calculated from the average pressure drop and the velocity in the porous media. Tortuosity, calculated by the ratio the average path length of the randomly injected massless particles to the thickness of the porous media, and the resultant effective diffusivity were in good agreement with the theoretical model. The suggested method can be used to calculate the parameters of real GDL accurately without any modification.

Evaluation of Wear of Periodontal Curet's Lateral Surface in Working-end (수종 치주큐렛의 작업측 마모도의 비교연구)

  • Shin, Dong-Whan;Lim, Sung-Bin;Chung, Chin-Hyung
    • Journal of Periodontal and Implant Science
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    • v.29 no.3
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    • pp.703-715
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    • 1999
  • The purpose of this study was to evaluate the degree of wear of periodontal curet's cutting edges made by three different manufacturers. In the first case of the experiment, this study was done with each new curett in the following three experiment. Twelve new double-ended No. 11/12 Gracy curettes from three different manufacturers (A, B and C) was randomly selected from our stock at first test. They were weared by wear tester with fixed pressure and limited distance. This study measured the width of worn curettes'cutting edge. The results were as follows ; 1)In 50 times experiment, this study discoverd that each manufacturer's curette was appeared with different degree of wear (p<0.01) 2)In 100 times experiment, this study result similarly to the 50 times experiment(p<0.01). 3)In 150 times experiment, the result of this study were alike as the X50 experiment and X100 experiment. In the second case of the experiment, the study was done with a curett of a manufacturer in the following three experiment. Two double-ended No. 11/12 Gracy curette from three different manufactures (A, B and C) was randomly selected from our stock at second test. 1)In 50 times experiment, this study discovered that each manufacturer's curette was appeared with different degree of wear (p<0.05). 2)In 100 times experiment, the result was same, compared with the 50 times experiment. 3)In 150 times experiment, this study also discovered that the result was same with the result of the upper two cases. In conclusion, this study discovered that the two cases of experimental were shown with a same result.

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Analysis Genetic Similarity of Gentiana scabra var. buergeri by Randomly Amplified Polymorphic DNA (RAPD를 이용한 용담의 유전적 유사도 분석)

  • Lee, Hae-Kyung;Lee, Mi-Kyung;Moon, Chang-Sik;Bang, Jae-Wook
    • Korean Journal of Medicinal Crop Science
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    • v.4 no.3
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    • pp.224-230
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    • 1996
  • Randomly amplified polymorphic DNA(RAPD) analysis was applied to detect the molecular polymorphisms in Gentiana scabra var. buergeri. A high level of molecular variability was found among wild plants and cultivars. In genetic analysis, eight of twenty primers were selected and 54 amplification products ranged 2. 2 to 0.2 kb were compared. Twenty - nine amplified products showed polymorphic, while five were monomorphic. Twenty of line specific bands were found. In genetic similarity and cluster analysis using PCR products, three wild plants collected from Naejangsan, Daedunsan and Keojedo and one cultivar Seochunjaerae were grouped into one cluster, while cultivar Jinbujaerae and Japanese line separated into another clusters, respectively. The identification of DNA polymorphisms by the RAPD technique will facilitate the selection of the lines from different origin.

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Effects of Replacing Soybean Meal with Fermented Rapeseed Meal on Performance, Serum Biochemical Variables and Intestinal Morphology of Broilers

  • Xu, F.Z.;Zeng, X.G.;Ding, X.L.
    • Asian-Australasian Journal of Animal Sciences
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    • v.25 no.12
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    • pp.1734-1741
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    • 2012
  • This trial was performed to study the effects of replacing soybean meal (SBM) with fermented rapeseed meal (RSM) on growth performance, serum biochemistry variable and intestinal morphology of broilers. A total of 640 d-old Arbor Acres broiler chicks were randomly allocated to 4 dietary treatments, 4 pens per treatment and 40 birds per pen for a 6-wk feeding trial. In the four treatment groups, fermented RSM replaced soybean meal at 0, 5, 10, and 15%, respectively. On 21 d and 42 d, two birds from each pen were randomly selected and slaughtered. Blood samples and sections of duodenum, jejunum, and ileum were collected for measurement of serum biochemical variables and intestinal morphology, respectively. Results showed that body weight gain (BWG) and feed conversion (FC) were significantly (p<0.01) poorer for birds fed the 15% fermented RSM diet than those fed with 0, 5 and 10% fermented RSM diets during all periods. Compared with 0 and 5% fermented RSM groups, IgG content in the serum of birds in 10 and 15% fermented RSM groups was improved (p<0.01) urea nitrogen content of serum was reduced (p<0.01) during both growing and finishing periods. However, IgM, phosphorus and calcium levels increased (p<0.05) only during the growing period. Increased (p<0.05) villus height was observed in the duodenum and jejunum of broilers fed the diet with 10% fermented RSM. In addition, villus height to crypt depth ratio in the jejunum was significantly higher (p<0.01) for birds fed the diet with 10% fermented RSM than for those fed diets with 0, 5 and 15% fermented RSM. The present results suggest that RSM fermented with Lactobacillus fermentum and Bacillus subtilis is a promising alternative protein source and that it could be safely used replace up to 10% SBM in broiler diets.

Intraspecific Diversity of Korean Takydromus wolteri(Reptilia: Squamata) Based on Randomly Amplified Polymorphic DNA (RAPD) Analysis (RAPD를 이용한 한국산 줄장지뱀(Reptilia: Squamata)의 종내 다양성에 관한 연구)

  • 장민호;송재영;정규회
    • Korean Journal of Environmental Biology
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    • v.22 no.2
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    • pp.295-299
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    • 2004
  • The lacertid lizard Takydromus wolteri widely distributed in South Korea, but intraspecific diversity of this species was not almost studied. T. wolteri represented by 8 specimens collected from 5 localities were analyzed by the Randomly Amplified Polymorphic DNA (RAPD) method with 28 random decamer primers and the phenogram constructed by using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method based on RAPD data. The populations of T. wolteri using in this paper were collected from Gyeonggi-do, Chungcheongbuk-do, Jeju-do, Jeollanam-do and Gyeongsangnam -do in South Korea. Among 68 different bands detected in RAPD analysis, 59 bands (87%) showed polymorphism. The phenogram showed that the populations of T. wolteri were grouped into two. These results suggest that T. wolteri were supported intraspecific diversity.

Genetic analysis of clubroot resistance in Chinese cabbage using single spore isolate of Plasmodiophora brassicae and development of RAPD marker linked to its resistance gene

  • Cho, Kwang-Soo;Hong, Su-Young;Han, Young-Han;Yoon, Bong-Kyeong;Ryu, Seoung-Ryeol;Woo, Jong-Gyu
    • Journal of Crop Science and Biotechnology
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    • v.11 no.2
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    • pp.101-106
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    • 2008
  • To identify inheritance of clubroot disease resistance genes in Chinese cabbage, seedling tests of $BC_1P_1,\;BC_1P_2$, and $F_2$ populations derived from $F_1$ hybrid(var. CR Saerona) using single spore isolate(race 4 identified with William's differential host) from Plasmodiophora brassciae were conducted. Resistance(R) and susceptible(S) plants segregated to 1:0 in backcross to the resistant parent. The $F_2$ population segregated in a 3(R):1(S) ratio. This result implied that the resistance of clubroot disease is controlled by a single dominant gene to the race 4 of P. brassicae in CR Saerona. To develop DNA markers linked to clubroot resistance genes, 185 plants of CR Saerona among $F_2$ populations were used. A total of 300 arbitrary decamer was applied to $F_2$ population using BSARAPD(Bulked segregant analysis-Randomly amplified polymorphic DNA). One RAPD marker linked to clubroot resistance gene in CR Saerona($OPJ_{1100}$) was identified. This marker was 3.1 cM in distance from resistance gene in $F_2$ population. This marker may be useful for a marker-assisted selection(MAS) and gene pyramiding of the clubroot disease resistant gene in Chinese cabbage breeding programs.

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Randomly Amplified Polymorphic DNA (RAPD) Analysis of the Lisianthus (Eustoma grandiflorum Shinn.) Variants Obtained during Tissue Culture (꽃도라지(Eustoma grandiflorum Shinn.) 조직배양시 발생한 변이체의 RAPD 분석)

  • Cheong, Chang Ho;Yu, Kee Won;Paek, Kee Yoeup
    • Horticultural Science & Technology
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    • v.17 no.3
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    • pp.352-354
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    • 1999
  • Randomly and specifically amplified polymorphic DNA band patterns based on polymerase chain reaction (PCR) analysis were used to assess genetic variation of somaclonal variants obtained from tissue culture of lisianthus (Eustoma grandiflorum). Five different types of variant were classified by morphological characters such as leaflet number, leaf shape, caulicle length, plant height, and leaf area. Five primers out of 20 primers (10 mer) resulted in 34 random amplified DNA fragments with polymorphisms (64.7%) in all tested plants. The dissimilarity coefficient was from 0.71 to 0.91 by UPGMA cluster analysis. Based on the presence of polymorphic bands, normal plant and five somaclonal variants were divided into two groups at the similarity coefficient value of 0.79.

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Development of Automatic Grading and Sorting System for Dry Oak Mushrooms -2nd Prototype- (건표고 자동 등급선별 시스템 개발 -시작 2호기-)

  • Hwang, H.;Kim, S. C.;Im, D. H.;Song, K. S.;Choi, T. H.
    • Journal of Biosystems Engineering
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    • v.26 no.2
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    • pp.147-154
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    • 2001
  • In Korea and Japan, dried oak mushrooms are classified into 12 to 16 different categories based on its external visual quality. And grading used to be done manually by the human expert and is limited to the randomly sampled oak mushrooms. Visual features of dried oak mushrooms dominate its quality and are distributed over both sides of the gill and the cap. The 2nd prototype computer vision based automatic grading and sorting system for dried oak mushrooms was developed based on the 1st prototype. Sorting function was improved and overall system for grading was simplified to one stage grading instead of two stage grading by inspecting both front and back sides of mushrooms. Neuro-net based side(gill or cap) recognition algorithm of the fed mushroom was adopted. Grading was performed with both images of gill and cap using neural network. A real time simultaneous discharge algorithm, which is good for objects randomly fed individually and for multi-objects located along a series of discharge buckets, was developed and implemented to the controller and the performance was verified. Two hundreds samples chosen from 10 samples per 20 grade categories were used to verify the performance of each unit such as feeding, reversing, grading, and discharging unites. Test results showed that success rates of one-line feeding, reversing, grading, and discharging functions were 93%, 95%, 94%, and 99% respectively. The developed prototype revealed successful performance such as the approximate sorting capability of 3,600 mushrooms/hr per each line i.e. average 1sec/mushroom. Considering processing time of approximate 0.2 sec for grading, it was desired to reduce time to reverse a mushroom to acquire the reversed surface image.

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Effects of Lumbar Stabilization Exercise Program on the Ratio of Abdominal and Back Muscle Strength in White Collar Workers

  • Kim, Bo-Ram;Park, Sun-Wook;Lee, Han-Suk
    • The Journal of Korean Physical Therapy
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    • v.27 no.6
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    • pp.419-424
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    • 2015
  • Purpose: This study aimed to evaluate the effects of lumbar stabilization exercise on the abdominal muscle-back muscle ratio in white-collar workers. Methods: Forty white-collar workers without any pain who worked at an information technology (IT) company located in the Gyeonggi province were included in this study. Of these, 20 subjects were randomly allocated to the lumbar stabilization exercise group, whereas the remaining 20 were randomly allocated to the control group. The lumbar stabilization exercise group performed a 40-minute-long exercise once a week for 8 weeks. The strength of the abdominal and back muscles was measured using the CENTAUR 3-D Spatial Rotation Device (BFMC, Germany). Data analysis was conducted using SPSS 18.0 for Windows. The Paired t-test was performed to compare the values measured before and after exercise within each group. The independent sample t-test was performed to analyze the differences between the 2 groups. The statistical significance for all the analyses was set at .05. Results: The strength of the abdominal muscles after the exercise significantly differed between the lumbar stabilization exercise group and the control group (p<0.05). However, the increase in the abdominal muscle-back muscle ratio was significant in only the lumbar stabilization exercise group (p<0.05), but not in the control group (p>0.05). Conclusion: The improvement of the abdominal muscle-back muscle ratio through lumbar stabilization exercise may have positive effects on lumbar stabilization. Moreover, lumbar stabilization exercise is considered beneficial for the prevention of back pain and musculoskeletal diseases.