• The Korean Journal of Zoology
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• v.31 no.1
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• pp.1-10
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• 1988

A syrnpatnc sample of Rana nigrnmaculata sand R plancyi (Anura) from Kwangu, Korea was investigated to clarify their taxonornic status and to estimate the degree of genic variation, genetic relatedness, and reproductive isolation between them, based on electrophoretic analysis, marphometric data, mating call, mating season, and habitat. All the characters measured including morphometric, genetic, behavioral, and ecological suggest that they are distinct species and R nigrnmaculata might have originated from R plancyi (tropical form about three and a half million years ago. An adult female was found to be Fl hybrid between two species The hybrid female possessed fettile eggs and morphologically intermediate to the parental species in head parts, hind limb, and web characters. The differences in mating call, mating behavior, breeding season, and habitat strongly suggest that postmating isolating mechanism is operating, even though very rare hybrid is found.

• Animal cells and systems
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• v.4 no.1
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• pp.45-50
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• 2000

We found a brown frog species, which is unrecorded from South Korea. Rana huanrenensis Fei, Ye, and Huang (1990), This species was originally described from northeastern China. In having 2n=24 chromosomes, this species is closely related to Rana dybowskii, R. chensinensis, R. ornativentris, R. pirica, and Chinese R. huanrenensis, but it is different from the first four species in the ecological, morphological, and genetic characteristics. By contrast, this species Is identical to Chinese R. huanrenensis In the habitat of montane stream-breeding, absence of the vocal sac, and genetic properties. This record is a significant range extention of R. huanrenensis.

• The Korean Journal of Zoology
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• v.3 no.2
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• pp.13-18
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• 1960

For the study on the sex ratio in natural populations of the different localities, the amphibian species were collected as the materials at the seven localities in Korea, and were obtained the following results. The sex ratios of each amphibian species suggest that each has it's own specific sex rations the following list. Rana nigromaculata H. : 109-39, Rana rugosa SCH. : 67.44, Rana temporaria coreana O. : 158.45, Hyla arborea japonica G. : 137.70. In Rana nigromaculata H. the average sex ratio was 109.39 as the above list, and its was reached statistically significant level according to the different localiies. The sex ratio was generally higher in the eastern area than that in the western area in this country. The sex ratio of the adult frogs of Rana nigromaculata H. was 135.18 , and it has a statistically significant level by each locality but the sex ratio of the younger frogs of the same species was 100.97 which was a little lower than that in the adult, and it was not laid at a significant level according to the localities. There is no doubt that the difference of sex ratios according to the localities was mainly due to the different ratios of the adult frogs which were effected on the constitution of sex by environmental factors in each locality.

• Animal cells and systems
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• v.4 no.1
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• pp.31-37
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• 2000

The genetic variations among six species of Rana from Korea (R. nigro-maculata, R. piancyi, R. dybowskii, R. sp, R. rugosa type A, B and R. amurensis) were investigated using 499 bases of mitochondrial DNA sequences for ND2 (NADH dehydrogenase subunit 2) gene and $tRNA^{Trp}$ gene. Partial sequences of ND2 gene (427 bp) and full sequences of $tRNA^{Trp}$ gene (73 bp) were identified. The level of sequence divergences ranged from 0.2 to 5.2% within species and 4.9-28.0% among 6 species of the genus Rana. The $tRNA^{Trp}$ gene of the genus Rana was composed of 77 nucleotides which showed a two dimensional "cloverleaf" structure. The secondary structure of $tRNA^{Trp}$ was not found compensatory changes which could potentially confound phylogenetic inference. In the neighborjoining tree, brown frogs were clustered first with the level of sequence divergence of 13.20% between R. amurensis and R. dybowskii, and 9% between R. dybowskii and R. sp. supported by 99% bootstrap iterations, respectively. R. nigromaculata and R. plancyi were clustered into another group with 5.1% divergence supported by 100% bootstrap iteration. R. rugosa A 8nd B types were grouped by 4.9% divergence and clustered into the last group with other two groups with 100% bootstrap iterations.

• Journal of Ecology and Environment
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• v.29 no.4
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• pp.331-335
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• 2006

We investigated the distribution of three anuran species, Three-striped pond frogs (Rana nigromaculata), Bullfrogs (Rana catesbeiana), and Narrow-mouthed toads (Kaloula borealis), in an administrative district, Haenam Gun, Junnam Province, Korea using volunteer call surveys. Twenty-eight volunteer call surveyors were assigned to each $2{\times}2km^2$ survey plot. Call surveys on whether the species are present or not were conducted for 5 minutes between 30 minutes after sunset and the midnight on rice fields and ponds from 10 April to 28 August in 2005. Depending on species, call surveys were carried out at seven to 28 plots with average 8.4 to 10.7 visits per the plot. We calculated the detection probabilities and occupancy rates of the three species using four models with three covariates: temperature, humidity, and the amount of water at the habitat. The model average detection probabilities of three anuran species of R. nigromaculata, R. catesbeiana, and K. borealis were 0.53, 0.74, and 0.41 respectively, and the site occupancy rates of them were 0.93, 0.94, and 0.86 respectively. Our results indicate that R. nigromaculata, R. catesbeiana, and K. borealis are common in Haenam Gun.

• Korean Journal of Environmental Biology
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• v.18 no.1
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• pp.113-120
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• 2000

Herpetofauna biodiversity was surveyed at the Chin-do. The results are as follows: During census periods presence of 2 orders, 4 families, 6 species of amphibians and 1 order, 2 suborders, 4 families, 8 species of reptiles were observed or found indirectly by auditorial recording from residents at the Chin-do. Among them 1 protected wildlife(Agkistrodon saxatilis) and several rare species such as Takydromus wolteri, Sciucella laterale laterale and Zamenis spinalis were indentified. Rana nigromaculata and Rana catesbeiana were found in all surveyed areas. Dominant amphibian species was Rana rugosa (D'= 25.8%) and reptile was Takydromus wolteri (D'= 21.8%). Among 4 areas survey area, Uisin-myon showed the highest level of species richness, diversity, and evenness indices (R'= 2.393, H'= 1.931, and E'= 0.7772, respectively). The species richness of Kunnae-myon was lower (R'= 0.932) than other surveyed regions. Based on the frequency of occurrence (85.71%), habitation density of Rana catesbeiana was very common in Chin-do, suggesting that spreading of this foreign species in the entire Korean peninsula. Scincella laterale laterale, Elaphe dione, Elaphe rufodorsata, Dinodon rufozonatus rufozonatus, Rhabdophis tigrinus tigrinus, Agkistrodon ussuriensis, and Zamenis spinalis showed low frequency of occurrence ( < 10%) and efforts to protect them are required. [Herpetofauna, Bio-diversity, Chin-Do].

• Journal of Environmental Impact Assessment
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• v.24 no.2
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• pp.175-189
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• 2015

The objective of this study was to analyze characteristics of distribution of amphibian species and the affecting ecological factors. For the study, habitat environment factors were determined and applied to a habitat suitability model for the data collected from the Seom River in Hoengseong County and Wonju City, Gangwon Province, Korea between March 2013 to October 2013. The analyzed amphibian species were Rana nigromaculata, Hyla japonica, Rana dybowski, and Rana rugosa Temminck and Schlegel, and a logistic regression model was used with the pseudo-absence data. The result of the model analysis suggests that the major factors for Rana nigromaculata are distance to vegetation and rock and that for Hyla japonica is waterway. Rana dybowski and Rana rugosa Temminck and Schlegel have similar habitat characteristics, but the latter is shown to be dominant due to its wider habitat preference. According to the species richness model, the analyzed amphibian species are shown to have tendency to move between valleys or streams. This study quantitatively analyzed habitat environment characteristics using species distribution model, however, there is a limitation in terms of analysis on food factor and connectivity with other species. Combined with additional density or habitat analysis on birds or fish, this study can lead to more comprehensive analysis on biological environment factors.

• The Korean Journal of Zoology
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• v.15 no.3
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• pp.105-110
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• 1972

A cellulosse acetate electrophoretic survey of Korean Anura has revealed the presence of diverse lactate and malate dehydrogenase (LDH and MDH) isozymes. The pattern of LDH and MDH isozymes in the tissues of the central nervous system of the six species of Anura examined are species specific and differ from those of mammals and birds. Both Rana nigromaculata nigromaculata and Rana nigromaculata coreana have two molecular forms of LDH and MDH, respectively, with almost the same pattern. Whole brain homogenate of Rana temporaria shows also a maximum of only two LDH isozymes. Both Bufo bufo asiaticus and Bombina orientalis have five molecular forms of LDH with an entirely different spacing on the zymograms, whereas Rana rugosa has three. Two molecular forms of MDH are present in all animals examined and one band is shown in olfactory lobe and mixture of cerebellum and medulla oblongata of Rana nigromaculata nigromaculata.

• Animal cells and systems
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• v.16 no.3
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• pp.260-267
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• 2012

Calling behavior is often used to infer breeding patterns in anurans. We studied the seasonal and diel calling activities of anuran species in a wetland in central Korea to determine the calling season and to evaluate the effects of abiotic factors on male calling. Acoustic monitoring was used in which frog calls were recorded for a full day, once a week, throughout an entire year. Using acoustic monitoring, we identified three frog species in the study site. Males of Rana dybowskii called in late winter and early spring; we thus classified this species as a winter/spring caller. The results of binary logistic regression showed that temperature, relative humidity, and 1-day lag rainfall were significant factors for male calling in R. dybowskii. Temperature and relative humidity were important factors for the calling activity of R. nigromaculata, whereas 24-h rainfall and 1-day lag rainfall were not significant. Thus, we determined R. nigromaculata to be a summer caller independent of weather. In Hyla japonica, relative humidity, 24-h rainfall, and 1- day lag rainfall were significant for male calling, suggesting that this species is a summer caller dependent on local rain.

• Journal of Species Research
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• v.7 no.4
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• pp.340-347
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• 2018

Four psammocinian species(Demospongiae: Dictyoceratida: Irciniidae) are described from Gageodo and Jejudo Islands, Korea. Among them, three are new species, Psammocinia rana, P. massa and P. vermins, which are very different from other recorded species. Secondary fibres of Psammocinia rana are mostly wide platelike shape with large perforation. Primary and secondary fibres of Psammocinia massa are lumped together in a thick mass through numerous areas. Psammocinia vemis is similar to P. mosulpia in skeletal structure, but differs in size of cored sands in primary fibres. The fourth species, we reclassify from Ircinia chupoensis to Psammocinia chupoensis based on more detailed observation of the skeletal structure. Ircinia only has sand cored primary fibres, while the observed species, has sand cored primary and secondary fibres.