• Near Infrared Analysis
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• 제1권2호
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• pp.9-20
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• 2000

Recently, near-infrared (NIR) spectroscopy and Raman spectroscopy have received keen interest as powerful techniques for nondestructive analysis of biological materials. The purpose of this review paper is to compare the advantages of NIR and Raman spectroscopy in the nondestructive analysis. Both methods are quite unique and often complementary. For example. NIR spectroscopy is very useful in monitoring in situ the content of components inside biological materials while Raman spectroscopy is very suitable for identifying micro-components on the surface of biological materials. In this article specific characters of the two spectroscopic methods are discussed first and then several examples of applications of NIR and Raman spectroscopy to the biological nondestructive analysis are introduced.

• Journal of Biosystems Engineering
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• 제42권3호
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• pp.170-189
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• 2017

Purpose: This paper presents Raman chemical imaging technology for inspecting food and agricultural products. Methods The paper puts emphasis on introducing and demonstrating Raman imaging techniques for practical uses in food analysis. Results & Conclusions: The main topics include Raman scattering principles, Raman spectroscopy measurement techniques (e.g., backscattering Raman spectroscopy, transmission Raman spectroscopy, and spatially offset Raman spectroscopy), Raman image acquisition methods (i.e., point-scan, line-scan, and area-scan methods), Raman imaging instruments (e.g., excitation sources, wavelength separation devices, detectors, imaging systems, and calibration methods), and Raman image processing and analysis techniques (e.g., fluorescence correction, mixture analysis, target identification, spatial mapping, and quantitative analysis). Raman chemical imaging applications for food safety and quality evaluation are also reviewed.

• 한국표면공학회지
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• 제57권2호
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• pp.71-85
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• 2024

This article provides an overview of Raman spectroscopy and its practical applications for surface analysis of semiconductor processes including real-time monitoring. Raman spectroscopy is a technique that uses the inelastic scattering of light to provide information on molecular structure and vibrations. Since its inception in 1928, Raman spectroscopy has undergone continuous development, and with the advent of SERS(Surface Enhanced Raman Spectroscopy), TERS(Tip Enhanced Raman Spectroscopy), and confocal Raman spectroscopy, it has proven to be highly advantageous in nano-scale analysis due to its high resolution, high sensitivity, and non-destructive nature. In the field of semiconductor processing, Raman spectroscopy is particularly useful for substrate stress and interface characterization, quality analysis of thin films, elucidation of etching process mechanisms, and detection of residues.

• Toxicological Research
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• 제34권2호
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• pp.127-132
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• 2018

Alcohol consumption triggers toxic effect to organs and tissues in the human body. The risks are essentially thought to be related to ethanol content in alcoholic beverages. The identification of ethanol in blood samples requires rapid, minimal sample handling, and non-destructive analysis, such as Raman Spectroscopy. This study aims to apply Raman Spectroscopy for identification of ethanol in blood samples. Silver nanoparticles were synthesized to obtain Surface Enhanced Raman Spectroscopy (SERS) spectra of blood samples. The SERS spectra were used for Partial Least Square (PLS) for determining ethanol quantitatively. To apply PLS method, $920{\sim}820cm^{-1}$ band interval was chosen and the spectral changes of the observed concentrations statistically associated with each other. The blood samples were examined according to this model and the quantity of ethanol was determined as that: first a calibration method was established. A strong relationship was observed between known concentration values and the values obtained by PLS method ($R^2=1$). Second instead of then, quantities of ethanol in 40 blood samples were predicted according to the calibration method. Quantitative analysis of the ethanol in the blood was done by analyzing the data obtained by Raman spectroscopy and the PLS method.

• 보존과학연구
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• 통권32호
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• pp.89-98
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• 2011

철 부식화합물은 부식인자들과 함께 다양한 결정구조를 가지며 2가지 이상의 부식화합물들이 혼합되어 존재하므로 각각의 특성을 밝혀내기가 매우 어렵다. 본 연구는 표준 철 부식물을 대상으로 Raman Micro-Spectroscopy 분석을 실시하여 표준 Raman Data 확보를 목적으로 수행하였다. 표준 철 부식물에 대한 신뢰성을 향상시키기 위하여 SEM-EDS 분석과 XRD 분석을 추가로 실시하였다. SEM-EDS 분석결과 모두 표준 철 부식물의 구성성분과 일치하였으나 Goethite의 경우 철 이외의 비철금속 성분이 검출되었다. XRD 분석 결과 모두 표준 철 부식물의 구조와 일치 하였으나 Iron sulfate($FeSO_4{\cdot}6H_2O$)의 경우 Rozenite($FeSO_4{\cdot}4H_2O$)로 확인되었다. Raman Micro-Spectroscopy 분석결과 기존에 연구되었던 수산화철, 산화철의 Wavenumber에서 새로운 peak이 발견되었는데 이는 레이저 파장의 변화에 따른 것으로 사료 된다. 염화철과 황산철에서 Wavenumber가 새롭게 확인되어 표준 Raman Data 8건을 확보하였다. Raman Micro-Spectroscopy는 적은양의 시료를 이용하여 물질의 특성과 구조를 비교적 간단하게 분석 할 수 있어 미세한 부분이나 시료의 양이 한정된 문화재에 활용도가 높을 것으로 판단된다.

• 분석과학
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• 제37권2호
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• pp.114-122
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• 2024

A transmission Raman spectroscopy-based quantitative model, which can analyze the content of a drug product containing naproxen sodium as its active pharmaceutical ingredient (API), was developed. Compared with the existing analytical method, i.e., high-performance liquid chromatography (HPLC), Raman spectroscopy exhibits high test efficiency owing to its shorter sample pre-treatment and measurement time. Raman spectroscopy is environmentally friendly since samples can be tested rapidly via a nondestructive method without sample preparation using solvent. Through this analysis method, rapid on-site analysis was possible and it could prevent the production of defective tablets with potency problems. The developed method was applied to the assays of the naproxen sodium of coated tablets that were manufactured in commercial scale and the content of naproxen sodium was accurately predicted by Raman spectroscopy and compared with the reference analytical method such as HPLC. The method validation of the new approach was also performed. Further, the specificity, linearity, accuracy, precision, and robustness tests were conducted, and all the results were within the criteria. The standard error of cross-validation and standard error of prediction values were determined as 0.949 % and 0.724 %, respectively.

• Carbon letters
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• 제9권2호
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• pp.127-130
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• 2008

This study aims to find a correlation between XRD and Raman result of the activated carbon fibers as a function of its activation degrees. La of the isotropic carbon fiber prepared by oxidation in carbon dioxide gas have been observed using laser Raman spectroscopy. The basic structural parameters of the fibers were evaluated by XRD as well, and compared with Raman result. The La of the carbon fibers were measured to be 25.5 ${\AA}$ from Raman analysis and 23.6 ${\AA}$ from XRD analysis. La of the ACFs were 23.6 ${\AA}$ and 20.4 ${\AA}$, respectively, representing less ordered through activation process. It seems that the $I_D/I_G$ of Raman spectra were related to crystallite size(La). Raman spectroscopy has demonstrated its unique ability to detect structural changes during the activation of the fibers. There was good correlation between the La value obtained from Raman and XRD.

• Current Optics and Photonics
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• 제1권4호
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• pp.402-411
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• 2017

Combined LIBS-Raman spectroscopy has been widely studied, due to its complementary capabilities as an elemental analyzer that can acquire signals of atoms, ions, and molecules. In this study, the classification of polymorphs was performed by laser-induced breakdown spectroscopy (LIBS) to overcome the limitation in molecular analysis; the results were verified by Raman spectroscopy. LIBS signals of the $CaCO_3$ polymorphs calcite and aragonite, and $CaSO_4{\cdot}2H_2O$ (gypsum) and $CaSO_4$ (anhydrite), were acquired using a Nd:YAG laser (532 nm, 6 ns). While the molecular study was performed using Raman spectroscopy, LIBS could also provide sufficient key data for classifying samples containing different molecular densities and structures, using the peculiar signal ratio of $5s{\rightarrow}4p$ for the orbital transition of two polymorphs that contain Ca. The basic principle was analyzed by electronic motion in plasma and electronic transition in atoms or ions. The key factors for the classification of polymorphs were the different electron quantities in the unit-cell volume of each sample, and the selection rule in electric-dipole transitions. The present work has extended the capabilities of LIBS in molecular analysis, as well as in atomic and ionic analysis.

• 한국항공우주학회지
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• 제46권6호
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• pp.519-526
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• 2018

우주 자원을 분석하기 위해서는 지구로 가져와야 한다는 단점이 있었다. 하지만, 레이저 유도 분해 분광법(Laser-induced breakdown spectroscopy, LIBS)고 라만 분광법(Raman spectroscopy)을 활용한다면 우주 광물의 실시간 정성 정량적으로 분석이 가능하다. 레이저 유도 분해 분광법은 높은 에너지의 레이저를 물질 표면에 집중시켜 플라즈마를 생성한후, 방출되는 빛을 분광기를 통해 획득하여 원자 구성을 분석하는 분광법이다. 라만 분광법은 레이저를 물질 표면에 조사시켜 산란되는 빛을 측정하여 분자구조를 분석하는 분광법이다. 이 두 가지 분광법은 각각 미지의 광물의 원자 분자를 분석하는 상호보완적인 분광법으로 우주탑재체로서 효율적인 장점을 가지고 있다. 본 연구에서는 주성분 분석법(Principal Component Analysis, PCA)을 활용하여 광물을 정성적으로 분석했다. 또한, 두 가지 광물을 혼합한 시료를 제작하여 구성 성분 비율에 따른 신호 세기로부터 물질의 구성 비율을 예측하는 정량분석을 시행하였다.

• BMB Reports
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• 제30권5호
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• pp.352-355
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• 1997

The surface adsorbed protein conformations onto the vaccine adjuvants were observed with a Raman spectroscopy by using the maximum adsorption conditions described previously. The adsorbed state Raman vibrational spectra and subsequent spectral analysis display no conformational changes for BSA or IgG relative to their native species in solution.