• Korean Journal of Food Science and Technology
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• v.54 no.1
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• pp.43-51
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• 2022

Regulation of the hair follicle cycle in association with dermal papilla cells is one of the most interesting targets for promoting hair regrowth. In this study, we examined whether steam-dried Betula platyphylla extracts (BPE) promote hair growth by upregulating in vitro and in vivo responses of dermal papilla cells. The data showed that BPE3 contained high amounts of phenolic compounds with higher antioxidant effects and increased hair growth-related genes, including fibroblast growth factor7 and Wnt7b, in dermal papilla cells. Notably, BPE3 effectively enhanced the formation of hair follicles by increasing FGF7, Wnt7b, and vascular endothelial growth factor in C57BL/6N dorsal skins. Additionally, BPE3 significantly decreased the expression of inflammatory repertoires, inducible nitric oxide synthase, interleukin-6, and cyclooxygenase 2. Several small molecules, such as betulin and unsaturated fatty acids, support the pharmacological activity of BPE3. In conclusion, BPE3 effectively promoted hair growth by activating dermal papilla cells and enhancing hair follicle cycles by attenuating the inflammatory environment in the scalp.

• Korean Journal of Veterinary Service
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• v.38 no.2
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• pp.127-136
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• 2015

A simultaneous determination method was developed for nine preservatives (benzoic acid, sorbic acid, dehydroacetic acid, methyl-, ethyl-, isopropyl-, propyl-, isobutyl- and butyl-parabens) in sausage by liquid chromatography with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS). Each parameter was established by multiple reaction monitoring in negative mode. Separation was achieved on a phenyl-hexyl ($2.5{\mu}m$, $2.1{\times}150mm$, Waters) with A-20 mM ammonium acetate containing 0.1% acetic acid in water, B-Acetonitrile as mobile phase with gradient mode at a flow rate of 0.3 mL/min. The developed method was validated for specificity, linearity, accuracy and precision in sausages samples. Linearity was over 0.998 with calibration curve of the mixed standards. The mean recoveries from sausages fortified at the level of 2.0~10.0 mg/L were in range of 98.60~109.16% with RSDs lower than 8.93%. The limits of detection (LOD) and the limits of quantification (LOQ) were in the range between 0.0003~0.085 mg/L and 0.01~0.257 mg/L, respectively. Intra-day precision and inter-day precision were 0.45~6.16% and 2.81~13.33%, respectively. Using presently developed determination method, 33 field sausage samples from Gwangju city in Korea were screened over nine preservatives. As a result, no preservatives were detected in all samples.

• Science of Emotion and Sensibility
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• v.13 no.2
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• pp.391-402
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• 2010

In this study, breathable waterproof materials were prepared by electrospinning. Five kinds of electrospun nanofiber web layered systems with different levels of nanofiber web density, as well as different substrates and layer structures were fabricated, and their mechanical properties (tensile, bending, shear, compression, surface, and thickness & weight) were measured by the KES-FB system and compared with those of conventional breathable waterproof fabrics (densely woven fabric, PTFE laminated fabric and PU coated fabric). The KES-FB measurements demonstrate that the lab-scale nanofiber web layered systems are more flexible and fuller than commercial nanofiber web layered systems, which have a more compact structure than the lab-scale nanofiber web layered systems. Densely woven fabrics and lab-scale nanofiber web layered systems showed lower values of tensile linearity (LT), bending stiffness (B), and shear stiffness (G) than those of PU coated and PTFE laminated fabric. These results indicate that they are more flexible and have less resistance to the shearing movement, corresponding to a more pliable material having a better drape, than PU coated fabrics and PTFE laminated fabrics.

• Journal of Acupuncture Research
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• v.19 no.5
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• pp.219-233
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• 2002

면역억제활성이 알려진 Cerves korean TEMMINCK var. manchur-icus Swinhoe(Nokyong) 약침(CPH)은 다모 뿔의 녹용을 열수추출한 용액이다. 본 연구에서는 녹용 약침의 효과를 adjuvant 유발 관절염 실험용쥐를 이용하여 골체, 골강도 및 골대체율의 감소를 평가하였다. 위의 골 대사 관련 검정실험을 위하여 6주령의 암컷 실험용쥐에 20일간 약물투여를 실시하였다. 실험적인 관절염유발은 실험용쥐의 뒤쪽 다리에 Adjuvant를 주사하여 유발시킨 결과, 요부의 골 무기질함량과 밀도(BMC, BMD) 그리고 압축강도는 관절염 실험용쥐에서 감소되었다. 10일 경과 후 골형성도(BFR/BS, BFR/BV)의 조직형태학적 기준척도인 혈청 osteocalcin 수가 정상대조군과 비교해 볼 때 현저하게 감소되었다. 그러나, 몸무게로 나눈 BMC치는 관절염 유발군과 정상군 사이에 큰 차이가 보이지 않았다. 그리고 골무기질 함량은 정상군에 비해 감소하지 않았다. 20일 경과후 몸무게로 나누거나 나누지 않은 BMC치 모두, 관절염 군에서 요부 몸체의 골무기질 함량과 강도가 정상군과 비교해 볼 때 현저하게 감소되었다. 잔존 소주(小柱)의 무기질 침착 표면은 현저하게 감소하였으며 파골 세포의 수는 증가하였다. 초기부터 매일 Shinsu(B23)에 CPH 약침 투여(10, 20, $50{\mu}g/kg$)는 20일 경과후 만성적인 다리 부종을 현저하게 방지하였으며, 골 무기질함량, 골강도 및 소주골 형성등의 감소와 파골세포수 증가도 완화하였다. Adjuvant주사로 장애를 받았던 연령대비 요부 길이 증가도 유지되었다. 이러한 결과는 Adjuvant에 의한 관절염 실험용쥐의 2차적인 골관절염에서 충분히 요추 몸체뼈와 강도 감소를 나타내기 위해서는 적어도 20일이 필요하다는 것을 제시하였다. 본 결과로부터 CPH가 실험용쥐의 관절염에 대한 골체, 골강도 및 골대체율을 조절하는데 유효하게 작용하고 있음을 알 수 있었다.

• Journal of Life Science
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• v.30 no.6
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• pp.532-541
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• 2020

In this study, we describe the inhibition of adipocyte differentiation by the lactic acid bacteria (LAB) fermentation product of Chrysanthemum indicum L. (CI) extract to control obesity. Preparation of LAB-fermented products was performed to overcome the cytotoxicity of CI extract. During fermentation and 3T3-L1 cell line experiment, cytotoxicity was not induced in the CI fermentation products over 1 day in culture. Fermented materials from highly proliferative cultures were selected for treatment of 3T3-L1 cells and for comparison with unfermented control groups. Cell survival and undifferentiated cell populations were decreased differentiation population in all experimental groups compared with controls, as measured using fluorescence-activated cell sorting analysis. Akt pathway activity increased upon treatment with these fermented extracts in 3T3-L1 cells. Gli2 depleted at the protein level in association with adipocyte differentiation. LAB KCTC 3115- and 3109-fermented extract treatment caused controlled Gli2 protein accumulation. Moreover, KCTC 3115 and 3109 were found to reduce C/EBPα and FAS was depleted, whereas pACC was increased at the protein level upon treatment with the fermentation products of each of the four LAB used in this study. With Lactococcus lactis subsp. lactis KCTC 3115 fermentation, the regulation of adipose differentiation and hedgehog signaling were also suppressed, thereby inhibiting the differentiation of progenitor cells. The basis for the activation of hedgehog signaling may provide insights into the treatment of obesity and the inhibition of adipocyte differentiation.

• Journal of Forest and Environmental Science
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• v.32 no.4
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• pp.329-343
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• 2016

The present study was conducted on ridge tops of moist temperate Oak forests in Garhwal Himalaya to assess the plant diversity, regeneration, biomass production and carbon assimilation in different Oak forests. For this purpose, three Oak forest types viz., (a) Quercus leucotrichophora or Banj Oak (FT1; between 1,428-2,578 m asl), (b) Quercus floribunda or Moru Oak (FT2; between 2,430-2,697 m asl) and (c) Quercus semecarpifolia or Kharsu Oak (FT3; between 2,418-3,540 m asl) were selected on different ridge tops in Bhagirathi catchment area of Garhwal Himalaya. A total of 91 plant species including 23 trees (8 gymnosperms and 15 angiosperms), 21 shrubs and 47 herbs species belonging to 46 families were recorded from all the ridge top Oak forests. The highest mean tree density ($607{\pm}33.60trees\;ha^{-1}$) was observed in Q. floribunda forest with lower mean total basal cover (TBC) value ($48.02{\pm}3.67m^2ha^{-1}$), whereas highest TBC value ($80.16{\pm}3.30m^2ha^{-1}$) was recorded for Q. semecarpifolia forest, with lowest mean stem density ($594{\pm}23.43stems\;ha^{-1}$). The total biomass density (TBD) across three Oak forests ranged between $497.32{\pm}83.70$ (FT1) and $663.16{\pm}93.85t\;ha^{-1}$ (FT3), while the total carbon density (TCD) values ranged between $228.75{\pm}22.27$ (FT1) and $304.31{\pm}18.12t\;ha^{-1}$ (FT3). Most of the tree species were found with good regeneration (GR) status (average 45%) in all the forest types whereas, few species were found not regenerating (NR) (average 17%) however, few new recruitments were also recorded. ANOVA (Post-Hoc Tukey's test at 5% significance level) indicated significant forest-wise differences in TBC, TBD and TCD (in tree layer); family and evenness (in shrub layer only) values, while insignificant differences were noticed in density values of tree, seedling and herb layer.

• Korean Journal of Food Science and Technology
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• v.45 no.6
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• pp.742-746
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• 2013

This study was conducted to investigate the effect of different steaming and drying temperature conditions on the physicochemical characteristics of pumpkin powder. All the samples were steamed for 30 or 60 s and then were dried using hot air at 40, 50, 60, and $70^{\circ}C$. The moisture content decreased with an increase in the hot-air drying temperature, while the soluble solid content and yield increased. The color values, L, a, and b of the pumpkin powder decreased with increasing hot-air drying temperature. The pumpkin powder, which was steamed for 60 s had the highest water absorption and water solubility indexes. The carotenoid content of pumpkin powder was mostly destroyed with the increase in the steaming and drying temperatures. The total sugar and reducing sugar contents increased with increasing steaming and drying temperatures. Thus, the steaming and dehydration temperature conditions influenced the physicochemical characteristics of the pumpkin powder.

• Korean journal of food and cookery science
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• v.29 no.6
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• pp.691-697
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• 2013

This study determines the processing suitability of pumpkin powder by assessing, the physicochemical properties of pumpkin powder subjected to different treatments: steaming, freezing and roasting. Pumpkin powder containing 13.61-16.05% moisture, 39.40-9.53% protein, 1.35-1.43% lipid, 0.26-0.50% ash, 5.01-5.65% yield, and 9.40-10.50 mg% sugar was used. In terms of color values, the L-value of control, a-value of the steamed sample, and b-value of the roasted sample waere the highest. The steamed sampled had the highest DPPH radical-scavenging activity. Reduction in sugar concentration varied significantly by treatments conditions (p < 0.05). Carotenoid concentration was the highest in control (19.75 mg%), followed by steamed sample (17.91 mg%), frozen sample (15.17 mg%), and the roasted sample (14.04 mg%). On the basis of these results, steamed pumpkin powder was identified as the most suited for instant processing.

• Asian-Australasian Journal of Animal Sciences
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• v.29 no.11
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• pp.1585-1592
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• 2016

The present experiment was conducted to evaluate the effect of simulated heat stress on digestibility and methane ($CH_4$) emission. Four non-lactating crossbred cattle were exposed to $25^{\circ}C$, $30^{\circ}C$, $35^{\circ}C$, and $40^{\circ}C$ temperature with a relative humidity of 40% to 50% in a climatic chamber from 10:00 hours to 15:00 hours every day for 27 days. The physiological responses were recorded at 15:00 hours every day. The blood samples were collected at 15:00 hours on 1st, 6th, 11th, 16th, and 21st days and serum was collected for biochemical analysis. After 21 days, fecal and feed samples were collected continuously for six days for the estimation of digestibility. In the last 48 hours gas samples were collected continuously to estimate $CH_4$ emission. Heat stress in experimental animals at $35^{\circ}C$ and $40^{\circ}C$ was evident from an alteration (p<0.05) in rectal temperature, respiratory rate, pulse rate, water intake and serum thyroxin levels. The serum lactate dehydrogenase, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase activity and protein, urea, creatinine and triglyceride concentration changed (p<0.05), and body weight of the animals decreased (p<0.05) after temperature exposure at $40^{\circ}C$. The dry matter intake (DMI) was lower (p<0.05) at $40^{\circ}C$ exposure. The dry matter and neutral detergent fibre digestibilities were higher (p<0.05) at $35^{\circ}C$ compared to $25^{\circ}C$ and $30^{\circ}C$ exposure whereas, organic matter (OM) and acid detergent fibre digestibilities were higher (p<0.05) at $35^{\circ}C$ than $40^{\circ}C$ thermal exposure. The $CH_4$ emission/kg DMI and organic matter intake (OMI) declined (p<0.05) with increase in exposure temperature and reached its lowest levels at $40^{\circ}C$. It can be concluded from the present study that the digestibility and $CH_4$ emission were affected by intensity of heat stress. Further studies are necessary with respect to ruminal microbial changes to justify the variation in the digestibility and $CH_4$ emission during differential heat stress.

• The Journal of the Acoustical Society of Korea
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• v.20 no.6
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• pp.34-39
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• 2001

An adaptive multi-rate (AMR) speech coder was adopted as a standard of W-CDMA by 3GPP and ETSI. The AMR coder is based on the CELP algorithm operating at rates ranging from 12.2 kbps down to 4.75 kbps, and it is a source controlled codec according to the channel error conditions and the traffic loading. In this paper, we implement the DSP S/W of the AMR coder using OakDSPCore. The implementation is based on the CSD17C00A chip developed by C&S Technology, and it is tested using test vectors, for the AMR speech codec, provided by ETSI for the bit exact implementation. The DSP B/W requires 20.6 MIPS for the encoder and 2.7 MIPS for the decoder. Memories required by the Am coder were 21.97 kwords, 6.64 kwords and 15.1 kwords for code, data sections and data ROM, respectively. Also, actual sound input/output test using microphone and speaker demonstrates its proper real-time operation without distortions or delays.