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Promotion effects of steam-dried Betula platyphylla extract on hair regrowth

자작나무 증포 추출물의 발모 촉진 효과

  • Ahn, Jeong Won (College of Life Science, Gangneung-Wonju National University) ;
  • Jang, Su Kil (College of Life Science, Gangneung-Wonju National University) ;
  • Jo, Bo Ram (College of Life Science, Gangneung-Wonju National University) ;
  • Kim, Hyun Soo (College of Life Science, Gangneung-Wonju National University) ;
  • Jeoung, Eui Young (College of Life Science, Gangneung-Wonju National University) ;
  • Hillary, Kithenya (College of Life Science, Gangneung-Wonju National University) ;
  • Yoo, Yeong Min (College of Life Science, Gangneung-Wonju National University) ;
  • Joo, Seong Soo (College of Life Science, Gangneung-Wonju National University)
  • 안정원 (강릉원주대학교 생명과학대학 생물의약신소재연구실) ;
  • 장수길 (강릉원주대학교 생명과학대학 생물의약신소재연구실) ;
  • 조보람 (강릉원주대학교 생명과학대학 생물의약신소재연구실) ;
  • 김현수 (강릉원주대학교 생명과학대학 생물의약신소재연구실) ;
  • 정의영 (강릉원주대학교 생명과학대학 생물의약신소재연구실) ;
  • 힐러리 키테냐 (강릉원주대학교 생명과학대학 생물의약신소재연구실) ;
  • 유영민 (강릉원주대학교 생명과학대학 생물의약신소재연구실) ;
  • 주성수 (강릉원주대학교 생명과학대학 생물의약신소재연구실)
  • Received : 2021.10.27
  • Accepted : 2022.01.03
  • Published : 2022.02.28

Abstract

Regulation of the hair follicle cycle in association with dermal papilla cells is one of the most interesting targets for promoting hair regrowth. In this study, we examined whether steam-dried Betula platyphylla extracts (BPE) promote hair growth by upregulating in vitro and in vivo responses of dermal papilla cells. The data showed that BPE3 contained high amounts of phenolic compounds with higher antioxidant effects and increased hair growth-related genes, including fibroblast growth factor7 and Wnt7b, in dermal papilla cells. Notably, BPE3 effectively enhanced the formation of hair follicles by increasing FGF7, Wnt7b, and vascular endothelial growth factor in C57BL/6N dorsal skins. Additionally, BPE3 significantly decreased the expression of inflammatory repertoires, inducible nitric oxide synthase, interleukin-6, and cyclooxygenase 2. Several small molecules, such as betulin and unsaturated fatty acids, support the pharmacological activity of BPE3. In conclusion, BPE3 effectively promoted hair growth by activating dermal papilla cells and enhancing hair follicle cycles by attenuating the inflammatory environment in the scalp.

본 연구에서는 자작나무 증포 추출물의 탈모 조절 활성 분석을 위해 in vitro (인간모유두세포) 및 in vivo (C57BL/6N 마우스) 모델을 이용하여 모발의 성장 효과를 평가하였다. 찌고 말리는 과정을 반복하는 증포 차수 별 함유 성분의 변화가 관찰되어 새로운 추출법의 가능성을 확인하였다, 즉, 1회-5회 증포 후 관찰된 성분의 변화는 3회 증포 추출물(BPE3)에서 안정적인 추출 수율, 높은 페놀화합물 함량 및 항산화 활성을 가지는 것으로 확인되었다. 또한, 발모 주기의 전 과정에 관여하는 모유두세포에 BPE3를 처리하였을 때 유의한 수준의 FGF7과 Wnt7b 발현을 증가시켜 모발 성장 촉진과 모발의 성장기 개시를 도울 것으로 판단되었다. In vivo 마우스 모델에 12일 간 BPE를 도포하여 관찰한 결과 6일 경과 시 양성대조군(MXD 및 PTN)과 유사한 수준으로 단모의 성장이 관찰되었으며, 9일 경과 시 높은 밀도의 발모가 진행되기 시작하여 12일 경과 시 미처리 대조군에 비해 BPE3군에서 고른 발모가 관찰되었다. H&E 염색을 통한 각 군별 피부조직의 변화는 BPE3군에서 뚜렷이 나타났으며, 특징적으로 단위면적 당 많은 모낭(hair follicle)의 형성과 모간부(hair shaft)의 신장이 관찰되어 안정적으로 모발의 성장기로 진입한 것으로 판단되었다. 피부조직의 유전자발현 추가 분석 시 FGF7, VEGF, 및 Wnt7b 유전자가 유의하게 증가하여 모발성장, 분화, 모낭줄기세포 활성을 유도하여 모발성장을 촉진시킨 것으로 생각된다. 또한, BPE3가 LPS로 유도된 RAW264.7 세포의 염증인자(iNOS, IL-6 및 COX2) 발현을 저해하여 자가면역 등 염증성 탈모억제에 긍정적 역할을 할 것으로 판단된다. GC-MS 분석을 통해 확인한 betulin과 불포화지방산 등 저분자 물질은 BPE3가 나타낸 약리활성을 방증하였다. 결론적으로, 자작나무 3회 증포 추출물인 BPE3는 모유두세포의 발모 주기를 촉진할 뿐 아니라 두피의 염증 환경에서 휴지기를 단축시켜 정상적 발모를 돕는 소재로서 높은 잠재력을 나타냈다.

Keywords

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