• Journal of Microbiology and Biotechnology
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• 제22권12호
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• pp.1613-1620
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• 2012

Bacterial wilt caused by Ralstonia solanacearum is a devastating disease of many economically important crops. Since there is no promising control strategy for bacterial wilt, phage therapy could be adopted using virulent phages. We used phage PE204 as a model lytic bacteriophage to investigate its biocontrol potential for bacterial wilt on tomato plants. The phage PE204 has a short-tailed icosahedral structure and double-stranded DNA genome similar to that of the members of Podoviridae. PE204 is stable under a wide range of temperature and pH, and is also stable in the presence of the surfactant Silwet L-77. An artificial soil microcosm (ASM) to study phage stability in soil was adopted to investigate phage viability under a controlled system. Whereas phage showed less stability under elevated temperature in the ASM, the presence of host bacteria helped to maintain a stable phage population. Simultaneous treatment of phage PE204 at $10^8$ PFU/ml with R. solanacearum on tomato rhizosphere completely inhibited bacterial wilt occurrence, and amendment of Silwet L-77 at 0.1% to the phage suspension did not impair the disease control activity of PE204. The biocontrol activities of phage PE204 application onto tomato rhizosphere before or after R. solanacearum inoculation were also investigated. Whereas pretreatment with the phage was not effective in the control of bacterial wilt, post-treatment of PE204 delayed bacterial wilt development. Our results suggested that appropriate application of lytic phages to the plant root system with a surfactant such as Silwet L-77 could be used to control the bacterial wilt of crops.

• The Plant Pathology Journal
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• 제23권1호
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• pp.22-25
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• 2007

Biocontrol activity of five strains of selected rhizo-bacteria were tested in tomato against bacterial wilt caused by Ralstonia solanacearum. After root bacterization the plants were grown in a perlite-hydroponic system. Upon challenge inoculation with the pathogen, all of the rhizobacterial strains efficiently suppressed the bacterial wilt in tomato in various rates, at maximum by the strain, Bacillus vallismortis strain EXTN-1. While the percent of infected plants in the non-bacterized control plants were 95%, it was only 65% in plants pre-treated with EXTN-1. It was also demonstrated that the movement of R. solanacearum within the stem was significantly hampered when the plants were root bacterized. As EXTN-1 has no antagonistic properties against R. solanacearum, the bacterial wilt was probably suppressed by a mechanism other than antibiosis. Previously, the strain had been proven to produce an efficient elicitor for inducing systemic resistance in many crops. As the present study confirmed that EXTN-1 has the ability for reducing the pathogen spread in tomato, the strain could be effectively used as a potential biocontrol agent against bacterial wilt.

• The Plant Pathology Journal
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• 제17권6호
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• pp.385.3-385
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• 2001

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.157-158
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• 2005

• 한국환경과학회지
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• 제21권6호
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• pp.695-704
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• 2012

The application of disinfection models on the plasma process was investigated. Nine empirical models were used to find an optimum model. The variation of parameters in model according to the operating conditions (first voltage, second voltage, air flow rate, pH) were investigated in order to explain the disinfection model. In this experiment, the DBD (dielectric barrier discharge) plasma reactor was used to inactivate Ralstonia Solanacearum which cause wilt in tomato plantation. Optimum disinfection models were chosen among the nine models by the application of statistical SSE (sum of squared error), RMSE (root mean sum of squared error), $r^2$ values on the experimental data using the GInaFiT software in Microsoft Excel. The optimum model was shown as Weibull+talil model followed by Log-linear+ Shoulder+Tail model. Two models were applied to the experimental data according to the variation of the operating conditions. In Weibull+talil model, Log10($N_o$), Log10($N_{res}$), ${\delta}$ and p values were examined. And in Log-linear+Shoulder+Tail model, the Log10($N_o$), Log10($N_{res}$), $k_{max}$, Sl values were calculated and examined.

• 식물병연구
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• 제13권1호
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• pp.1-5
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• 2007

경상북도 북부지역 7개 시 군의 담배재배 농가를 대상으로 병해 발생상황과 농민들의 병해방제법과 관련된 재배방법을 조사하였다. 육묘기와 본밭초기의 담배에서 발생되는 전염성 병해는 극히 미미하였으며, 수확기의 담배에 가장 피해를 주는 병은 감자바이러스 Y에 의한 모자이크병과 세균성마름병(Ralstonia solanacearum)이었다. 담배모자이크바이러스(TMV)에 의한 모자이크병은 저항성 품종의 확대 보급으로 인하여 10년 전에 비하여 크게 감소되었으며, 세균성마름병 발생은 감수성 품종 보급으로 인하여 증가하였다. 30% 이상의 담배 재배 농민들이 농약을 오용하고 있었으며, 같은 수의 농민들이 10년 이상 담배를 연작하고 잘못된 윤작 작물을 선택하였다.

• The Plant Pathology Journal
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• 제38권3호
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• pp.203-211
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• 2022

Bacterial wilt, which is a major soil-borne disease with widespread occurrence, poses a severe danger in the field of tobacco production. However, there is very limited knowledge on bacterial wilt-induced microecological changes in the tobacco root system and on the interaction between Ralstonia solanacearum and fungal communities in the rhizosphere soil. Thus, in this study, changes in fungal communities in the rhizosphere soil of tobaccos with bacterial wilt were studied by 18S rRNA gene sequencing. The community composition of fungi in bacterial wilt-infected soil and healthy soil in two tobacco areas (Gengma and Boshang, Lincang City, Yunnan Province, China) was studied through the paired comparison method in July 2019. The results showed that there were significant differences in fungal community composition between the rhizosphere soil of diseased plants and healthy plants. The changes in the composition and diversity of fungal communities in the rhizosphere soil of tobaccos are vital characteristics of tobaccos with bacterial wilt, and the imbalance in the rhizosphere microecosystem of tobacco plants may further aggravate the disease.

• The Plant Pathology Journal
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• 제38권6호
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• pp.692-699
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• 2022

Bacterial wilt caused by Ralstonia solanacearum is considered one of the most harmful diseases of pepper plants. Recently, research on plant disease control through the rhizosphere microbiome has been actively conducted. In this study, the relationship with disease occurrence between the neighboring plant confirmed by analyzing the physicochemical properties of the rhizosphere soil and changes in the microbial community. The results confirmed that the microbial community changes significantly depending on the organic matters, P₂O₅, and clay in the soil. Despite significant differences in microbial communities according to soil composition, Actinobacteriota at the phylum level was higher in healthy plant rhizosphere (mean of relative abundance, D: 8.05 ± 1.13; H: 10.06 ± 1.59). These results suggest that Actinobacteriota may be associated with bacterial wilt disease. In this study, we present basic information for constructing of healthy soil in the future by presenting the major microbial groups that can suppress bacterial wilt.

• 식물병연구
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• 제11권2호
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• pp.193-197
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• 2005

수경재배에 있어 배양액의 pH가 Ralstonia solanaceaum 의 토마토 뿌리내의 이동과 배양액 전체로의 전염 확산에 미치는 영향을 조사하였다. 실내실험에서 배앙액의 pH를 4.0 또는 4.5로 조절한 배양액을 24시간 방치하였을 경우에는 병원균이 생존할 수 없었으며, pH를 각각 5.0, 5.5, 6.0, 6.5로 조절한 경우에는 1, 14, 51,그리고 $62\%$의 생존율을 보였다. pH 6.5와 5.0의 두가지 pH조건하에서 포장내 NFT(nutrient-film technique)배양에서 풋마름병원균 밀도는 pH 6.5 보다 pH 5.0에서 더 현저하게 낮았다. 모든 데이터에서 풋마름병원균은 pH 6.5에서 현저하게 시들음 증상의 발병율이 높게 발생하였다. 뿌리에 접종된 토마토 포장에서는 pH 5.0 처리구(l1/48) 보다 pH 6.5 처리구에서 현저하게 발병율이 높았다(34/48).줄기에 접종된 병원균은 pH 6.5에서 24주중 7주가 시들음 현상을 나타내었다. 수경재배에 있어서 pH 환경을 조절하여 토마토 시들음병에 잠재적인 역할을 하는 풋마름병의 발병율을 억제할 수 있었다.

• Mycobiology
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• 제43권3호
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• pp.311-318
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• 2015

Culture filtrates of six different edible mushroom species were screened for antimicrobial activity against tomato wilt bacteria Ralstonia solanacearum B3. Hericium erinaceus, Lentinula edodes (Sanjo 701), Grifola frondosa, and Hypsizygus marmoreus showed antibacterial activity against the bacteria. Water, n-butanol, and ethyl acetate extracts of spent mushroom substrate (SMS) of H. erinaceus exhibited high antibacterial activity against different phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum, Agrobacterium tumefaciens, R. solanacearum, Xanthomonas oryzae pv. oryzae, X. campestris pv. campestris, X. axonopodis pv. vesicatoria, X. axonopodis pv. citiri, and X. axonopodis pv. glycine. Quantitative real-time PCR revealed that water extracts of SMS (WESMS) of H. erinaceus induced expressions of plant defense genes encoding ${\beta}$-1,3-glucanase (GluA) and pathogenesis-related protein-1a (PR-1a), associated with systemic acquired resistance. Furthermore, WESMS also suppressed tomato wilt disease caused by R. solanacearum by 85% in seedlings and promoted growth (height, leaf number, and fresh weight of the root and shoot) of tomato plants. These findings suggest the WESMS of H. erinaceus has the potential to suppress bacterial wilt disease of tomato through multiple effects including antibacterial activity, plant growth promotion, and defense gene induction.