• The Journal of Internal Korean Medicine
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• v.21 no.2
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• pp.275-281
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• 2000

Object : This study was carried out to examine the effect of Bupleuri Radix on experimental cholestasis, and make clear apart of this mechanism. Methods : Two models of common bile duct ligation group and taurocholate load group after common bile duct ligation were induced, and Bupleuri Radix extract was taken orally for 14 days. In the 1, 2, 4, 7 and 14 days after treatment, the mitochondrial and microsomal monoamine oxidase(MAO) A and B activities in liver were measured. Results : The mitochondrial MAO A and B activities increased in both Blupleuri Radix treated group after common bile duct ligation and Blupleuri Radix treated group after taurocholate load and common bile duct ligation. MAO A increased in Blupleuri Radix treated group after taurocholate load and common bile duct ligation, and MAO B increased in Blupleuri Radix treated group after common bile duct ligation. The microsomal MAO A activities increased in both Blupleuri Radix treated group after common bile duct ligation and Blupleuri Radix treated group after taurocholate load and common bile duct ligation. Conclusion : According to the result, it is consider that Blupleuri Radix not only improves cholestatis in liver, but also decreases a genetic synthesis of taurocholic acid.

• Journal of Acupuncture Research
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• v.19 no.3
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• pp.51-63
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• 2002

Objective : The purpose of this study is to investigated that effect of Bupleuri Radix Herbal-acupuncture solution (BRHS) on the celluar immune response in mice with adjuvant induced arthritis, performed several experimental items : those are paw edema, IL-1b, IL-6, IL-8, PNF-a and $PGE_2$. Methods : All the male Sprague Daeley mice used in this study were bred and maintained in our pathogen-free mouse colony and were 8 weeks of age at the start of the experiment. The experimental model of arthritis was induced by injection of $50{\mu}g/{\mu}{\ell}$ adjuvant(mineral oil mixed Mycobacterium butyricum). Bupleuri Radix Herbal-acupuncture solution (BRHS) was injected into $ST_{36}$(足三理) of mice daily for 21 days. Immunohistological analysis was carried out to assess paw edema, IL-$1{\beta}$, IL-6, IL-8, PNF-${\alpha}$ and $PGE_2$expression in synovial membrance and sera Bupleuri Radix Herbal-acupuncture solution(BRHS) injected. Results : At day 21 post arthritis onset, immunohistological studies using monoclonal antibodies showed that Bupleuri Radix Herbal-acupuncture solution (BRHS) group had decreased expression of IL-$1{\beta}$, IL-6, IL-8, PNF-${\alpha}$ and $PGE_2$ at inflamatory cytokines production and edema compared with control group. Conclusion : Bupleuri Radix Herbal-acupuncture solution (BRHS) inhibited infammatory cytokines production and edema in adjuvant induced arthritic mice. Thus, Herbal-acupuncture solution may have prevention.

• Natural Product Sciences
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• v.24 no.3
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• pp.164-170
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• 2018

Rapid geographical classification of Bupleuri Radix is important in quality control. In this study, near infrared spectroscopy (NIRS) combined with supervised pattern recognition was attempted to classify Bupleuri Radix according to geographical origins. Three supervised pattern recognitions methods, partial least square discriminant analysis (PLS-DA), quadratic discriminant analysis (QDA) and radial basis function support vector machine (RBF-SVM), were performed to establish the classification models. The QDA and RBF-SVM models were performed based on principal component analysis (PCA). The number of principal components (PCs) was optimized by cross-validation in the model. The results showed that the performance of the QDA model is the optimum among the three models. The optimized QDA model was obtained when 7 PCs were used; the classification rates of the QDA model in the training and test sets are 97.8% and 95.2% respectively. The overall results showed that NIRS combined with supervised pattern recognition could be applied to classify Bupleuri Radix according to geographical origin.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.19 no.2
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• pp.40-49
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• 2006

Background and Objectives : In oriental Medicine, there is a concept that herb works only in it's own specific site called 'Guikyung(歸經)'. If two herbs have same effect and different Guikyung, two herbs would work at different site respectively. so we want to prove that whether Guikyung has significance or not in experimentally. Methods : In order to investigate the effects of Victicis Fructus & Bupleuri Radix on the Allergic rhinitis, BALB/c mouse was used. Control and experimental group 1, 2 were induced allergic rhinitis. Experimental group 1 was administered Victicis Fructus for 28 days and group 2 was administerd Bupleuri Radix for same period. WBC, neutrophil, lymphocyte, monocyte, IL-4 and $IFN-{\gamma}$ were measured and histological examination was performed. The statistical significance was examined by Independent Samples T test. Results : 1. WBC, neuoophil, lymphocyte, monocyte, IL-4 and $IFN-{\gamma}$ showed no statistically significant differences in three groups. 2. Histological examination had no differences in three groups. Conclusion: Victicis Fructus & Bupleuri Radix have no effect on allergic rhinitis and the reason is estimated that Guikymg plays a important roll to determine the effect of herb from the viewpoint of oriental medicine.

• Korean Journal of Pharmacognosy
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• v.16 no.4
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• pp.253-258
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• 1985

Various determination methods of saikosaponins in Bupleuri Radix are discussed, and the content of each saikosaponin which is original and/or artifact is rearranged. The changes of saikosaponin a and d into saikosaponin $b_1\;and\;b_2$, respectively, under gastric pH, were studied.

• Herbal Formula Science
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• v.19 no.1
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• pp.161-174
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• 2011

Objectives : The effects of Extraction after Mixture of Bupleuri Radix and Lycii Radicis Cortex on the change of the HPA-Axis system and the Catecholamic system was investigated. Methods : After performing the Forced Swimming Test(FST), the expressions of corticotropin releasing factor(CRF), c-Fos in the paraventricular nucleus(PVN), and tyrosine hydroxylase(TH) in the ventral tegmental area(VTA) and locus coeruleus(LC) were measured by immunohistochemical method. Results : The duration of immobility in FST was significantly decreased in A 100(Extraction after Mixture of Bupleuri Radix and Lycii Radicis Cortex, 100mg/kg) and A 400(Extraction after Mixture of Bupleuri Radix and Lycii Radicis Cortex, 400mg/kg)(p<0.001). The expression of CRF in the PVN was shown the tendency to reduce in A100 and A400. The expression of c-Fos in the PVN was shown the tendency to reduce in A100 and A400. The expression of TH in the VTA was shown the tendency to reduce mildly in A100 and A400. The expression of TH in the LC was significantly reduced in A400(p<0.001). And the dose dependent reduction tendency was shown, respectively. Conclusions : According to the results above mentioned, the immobility, c-Fos and CRF expression was reduced at lower dose and was increased at higher dose. Therefore there is contradictory effects on the HPA Axis system in accordance with the dose. But in the effects on the catecholaminergic system, it significantly reduced the expression of TH in the LC. It was validated that the effect on the catecholaminergic system was ruled by Bupleuri Radix rather than Lycii Radicis Cortex via mainly the noradernergic system.

• Korean Journal of Pharmacognosy
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• v.32 no.1 s.124
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• pp.72-78
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• 2001

This study was performed to elucidate the influence on the plasma decursinol concentration after administration of combined decursin and Cnidii Rhizoma or decurisin and Bupleuri Radix extracts in comparison with single decursin administration. The identification of decursinol isolated from Angelica gigas was carried out by GC/MS and the concentration of decursinol in the plasma after oral administration was determined by HPLC. The value of area under the plasma concentration (AUC) and the maximal concentration (Cmax) of decursinol after administration of methanol and ether Angelicae Gigantis Radix extract was higher than those of single decursin-treated group. It was also found that the AUC and the Cmax of decursinol after combined administration with decursin and Cnidii Rhizoma were higher than those of decursin administration, whereas those of combined with decursin and Bupleuri Radix was lower. Moreover, the studies were performed with decursinol only or with combined decursinol and Cnidii Rhizoma/Bupleuri Radix, both of combined treatment increased the plasma decursinol concentrations compared with decursinol-treated group in rats. On the basis of these results, it is concluded that co-existing substances or co-administration with other drugs may influence the plasma levels of decurisinol after oral administration.

• YAKHAK HOEJI
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• v.39 no.1
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• pp.14-23
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• 1995

The current study was undertaken to select the crude drug showing life prolonging effect on the starved rats(Sprague-Dawley) and elucidate the reaction mechanism there of. As distilled water extracts prepared from twenty kinds of natural drugs were administered orally to female starved rats, Bupleuri Radix demonstrated the most prominent effect with 34.6% increment in the survival time. To investigate underlying mechanism of life prolonging effect of Bupleuri Radix, the concentrations of adrenocorticosteroids(corticsterone, testosterone and aldosterone) and enzyme activities of superoxide dismutase(SOD), glutamate oxaloacetate transaminase(GOT), glutamate pyrurate transaminase(GPT) were assayed in the serums of rats starved for, 2, 4 and 6 days respectively. The results manifested much elevated values of corticosterone, aldosterone(2 days) and rather decreasing tendency afterwards, specially in the late periods of starvation(6 days). With respect to such a considerable changes according to starvation periods, Bupleur Radix restored these values much near to normal suggesting that Buplerum Radix may play a life prolonging action during starvation probably through reinforcing the homeostatic properties of corticosteroids and some enzyme activities.

• Korean Journal of Plant Resources
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• v.11 no.3
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• pp.283-289
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• 1998

To determine the differences among cultivated , non-cultivated and imported bupleurum falcatum, contents and compositions of fatty acids and saikosaponins which are known as a major biologically acitive compounds in Bupleurum falcatum were meausred with GC and HPLC. Color of grinded powder of Bupleuri radix determined by colorimeter showed that the imported one has the highest white and yellow coloer Among the Bupleuri radix cultivated in China, Hong-siho(red-Bupleurum) produced in Yungil Province contained the highest red color. Cultivated Korean Bupleurum radix contained higher saikosaponi a, c, and than the imported ones which were cultivated in Yungil, Ankuk , and Gillium Province in China. Levels of saikosaponin c are 3 times higher in cultivated Korean Bupleuria radix compared the with imported ones. Furthermore, Bupleurum falcatum cultivated in Korea contained higher amount of palmitic and linoleic acids than those of improted and non-cultivated ones. Particularly they contain two to six times higher linoleic acid.

• Herbal Formula Science
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• v.17 no.2
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• pp.1-20
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• 2009

The purpose of this study was to examine how exterior-relieving herbs are blended by each prescription with emphasis on Internal disease range of Donguibogam by limiting Wind herbs in exterior-relieving herbs to apply exterior-relieving herbs to internal diseases. Prescriptions mixed with exterior-relieving herbs is a prescription adding Bojungikgitang, Yijintang, Yimyosan, Yukilsan and Gilgyeongjigaktang. The mixed exterior-relieving herbs were diversely operated according to each prescription or medicines. However, the mixed exterior relieving herbs were mixed for rising yang & tonifying qi(升陽益氣) for the most part. The exterior-relieving herbs mixed with prescriptions had the total 15 kinds, Bupleuri Radix, Cimicifugae Rhizoma among them were mixed with prescriptions for the most part. In addition, two-kind mixed exterior-relieving herbs were utilized in order of Bupleuri Radix Cimicifuga Rhizoma and Bupleuri Radix Cimicifugae Rhizoma Notopterygii Rhizoma Ledebouriellae Radix. Taken together, Wind herbs is applied several purpose, so that it can contribute to treatment and prevention of internal diseases in present-day.