• 산업과 과학
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• 제2권3호
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• pp.1-7
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• 2023

최근 방사능 노출에 대한 염려에 대한 이슈가 높아지고 있다. 토양, 물, 공기, 작물등에 영향을 주며 장기적으로 환경오염 및 식량오염이 발생하며 나아가 사회적인 혼란 및 경제적 타격을 초래할 것으로 여겨진다. 방사능 노출로 질병을 일으키기도 하지만, 질병진단을 위한 방법으로, X선촬영, CT, PET-CT등 핵의학 검사를 실시하고, 암치료 목적으로 방사선 동위원소에 노출시키기도 한다. 후쿠시마 방사능 폐기물 방류소식으로 물, 특히 식수에 포함되는 방사선에 대한 헝가리의 사례 연구 및 남극 대륙의 Larsemann Hills 지역 검사에서 세계 보건 기구에서 권장하는 음용수의 규정된 방사능 한계 내에 있었다. DNA손상, 세포 및 장기손상, 암에 관련된 내용을 중심으로 방사선 보호제를 살펴보고, 또한 복구물질중 ACE억제제, 항산화제, 천연물질등에 관하여 여러 문헌을 조사하였다. 일상에서 방사능에 노출되지만 안전할 수 있는 이유는 아마도 방사선보호물질, 방사능 피폭에 대한 복구 물질이 있을 것으로 여겨, 가능한 물질들을 찾아보고자 한다.

• Journal of Radiation Protection and Research
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• 제48권1호
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• pp.1-8
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• 2023

Background: The purpose of this study is to evaluate the immunosuppressive and antioxidant effects of a novel radioprotective agent using the vitamin E derivative 2-(alpha-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol (TMG) and its effect on tumors, and to study its usefulness. Materials and Methods: In this study, C57BL/6NCrSlc mice were divided into four groups (control, TMG, radiation therapy [RT], and RT+TMG), using 10 mice in each group. In the TMG and 2 Gy+TMG groups, 500 mg/kg TMG was administered. Two groups (2 Gy and 2 Gy+TMG) among RT and RT+TMG groups were irradiated with 2 Gy in a single fraction, while the other two groups (6 Gy and 6 Gy+TMG) were irradiated locally with 6 Gy in three fractions. Results and Discussion: TMG positively affected CD4+ and CD8+ T lymphocytes. Tumor volumes and growth inhibition rates were compared. In order to evaluate how TMG administration affected tumor growth, Ehrlich cancer cells were injected into the thigh of mice, and the tumor volume and growth suppression rate were compared. Not only RT but also TMG alone inhibited tumor growth. If RT conducted to the mice with TMG, TMG could increase the number of leukocytes, primarily that of lymphocytes. TMG also inhibited tumor growth in addition to RT. Tumor growth was significantly inhibited in the 6 Gy+TMG group. Conclusion: In conclusion, TMG exerted an immunopotentiating effect mainly by increasing the white blood cell numbers including that of lymphocytes. In addition to RT, TMG also inhibited tumor growth. Therefore, TMG is considered to be a useful radioprotective agent in radiotherapy without tumor growth induction.

• 대한한방종양학회지
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• 제3권1호
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• pp.129-147
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• 1997

현재 서양의학에서 활용되고 있는 항암 요법으로는 외과처치(外科處置), 화학요법(化學療法), 방사선요법(放射線療法) 및 면역요법(免疫療法) 등(等)이 있으며, 그 중에서도 항암제(抗癌劑)에 의한 화학요법과 방사선요법이 가장 많이 응용되고 있다. 그런데 이들은 암세포(癌細胞)뿐만 아니라 정상세포(正常細胞)까지도 살상(殺傷)함으로써 종양세포(腫瘍細胞)를 억제하는 동시에 골수조혈기(骨髓造血器), 소화계통(消化系統) 및 면역기능(免疫機能)을 포함하는 인체의 정상적인 기능을 손상시키는 문제점으로 그 사용에 제한을 받고 있다. 따라서, 본 연구는 삼용부정탕(蔘茸扶正湯)의 항암요법으로 손상된 조직의 회복 및 조혈촉진 효과를 규명하기 위해 생쥐를 대상으로 하여 실험하였다. 그 결과, 생쥐 비장세포에 대한 증식효과(增殖效果), 조혈촉진인자(造血促進因子)의 분필능(分泌能), 방사선에 대한 임파구의 방어효과(防禦效果), 방사선에 대한 조혈세포 방어효과(防禦效果), 방사선(9Gy)을 조사받은 생쥐의 생존율(生存率) 등에서 유의성 있는 결과를 얻어내어 아래와 같이 보고하는 바이다.

• Journal of Ginseng Research
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• 제15권3호
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• pp.167-170
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• 1991

Radiation protective fraction was Isolated and partially purified from Korean white ginseng. The effect of the fraction was studied on the cell survival of W-damaged CHO-Kl cells. As a result, it was found that the fraction increased the survival rate of damaged cells significantly within the dose range of which cytotoxicity did not appear This fraction was separated into two parts by adding butanol, namely the precipitated protein component and the butanol extract. Damaged cells were treated with each of these components and their survival rates were measured. The protein component demonstrated significant increase in the survival rates, while the butanol extract showed no such increment. These results suggest that the radiation protective effect of the ginseng fraction is originated from the butanol-precipitated protein component, not from the butanol-soluble compounds.

• 혜화의학회지
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• 제20권1호
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• pp.175-182
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• 2011

This study aims to summarize and make a reference of Sagunjatang and Samultang by investigating and comparing the effects of prescriptions. In this process, this review surveyed all papers used Sagunjatang and Samultang in PubMed database and finally 36 papers were included. The publication year, the nations of authors, the type of materials, the formation of experiments, the type of herbal medicine, and their action and mechanisms were analyzed. The number of studies on Sagjunjatang and Samultang has increased since 1990's. The main formation of experiments was in vivo study and the portion was 55%. The proportion of the research using prescriptions was 71%. Sagunjatang was investigated in the field of GI track function, anticancer effect, radioprotective effect and etc. Samultang was studied in the field of anticancer effect, brain cognitive function, anti-inflammatory effect, cell protective effect, and etc. Based on these results, Sagunjatang and Samultang showed the different bioactivities. This study will provide the useful information on differentiation and analysis of the effect of herbal prescriptions by the theory of Korean traditional medicine.

• 약학회지
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• 제30권6호
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• pp.343-347
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• 1986

Korean ginseng was purified to obtain radioprotective protein fractions by buffer extraction, ammonium sulfate fractionation, CM-cellulose column chromatography, heat inactivation and Sephadex G-75 column chromatography. The final three fractions, GI, GII and GIII were subjected to Disc-polyacrylamide gel electrophoresis (PAGE) and SDS-PAGE. The molecular weights(M.W.) of native and denatured proteins were estimated by using regression line equations obtained from the mobilities of standard proteins. As the results, in Disc-PAGE, the GI fraction showed two protein bands with M.W. of above 213, 000 and 55, 000, GII showed one band with M.W. of 44, 000 and GIII, also one band with M.W. of 19, 000. In SDS-PAGE, GI fraction gave four subunit bands with M.W. of above 114, 000, 27, 000, 24, 000 and 19, 000, GII gave two bands with M.W. of 46, 000 and 22, 000, and GIII, one band of 19, 000.

• Archives of Pharmacal Research
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• 제9권1호
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• pp.5-9
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• 1986

Partialy purified ginseng proteins were either treated with sodium dodecyl sulfate (SDS) and .betha.-mercaptoethanol to denature the proteins or not, and subjected to Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography (HPLC) to compare the components of each fraction. Standard proteins of known molecular weights (MW) were also either treated with SDS and .betha.-mercaptoethanol or not, and subjected to HPLC to obtain regression lines for MW determination. From the retention times obtained from samples in eiether case by HPLC, the MW were estimated as following in SDS treated condition, Gl fraction showed three peaks each with MW of above 100, 000, 51, 000 and 19, 000. Gll showed one original peak with MW of 21, 000 and Gll, two peaks each with MW of 19, 000 and 14, 000. On the other hand, in non-SDS treated condition, GI fraction showed two peaks each with MW of above 200, 000 NA 52, 000. Gll showed one original peak with MW of 41, 000 and Gill, three peaks each with MW of 28, 000, 19, 000 and 14, 000.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2000년도 추계학술대회 및 정기총회
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• pp.21-21
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• 2000

We performed this study to determine the effect of Bu-Zhong-Yi-Qi-Tang, as a prescription of traditional Oriental medicine, and its major ingredients on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of r-radiation. Bu-Zhong-Yi-Qi-Tang administration before irradiation protected the jejunal crypts (p<0.0001), increased the formation of endogenous spleen colony (p<0.05) and reduced the frequency of radiation-induced apoptosis (p<0.05). (omitted)

• 대한방사선기술학회지:방사선기술과학
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• 제23권1호
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• pp.77-80
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• 2000

전신 ${\gamma}$-선 조사전 Grifola umbellata(Gu) 추출물의 단일 투여가 생쥐의 생존율, 체중, 기관 무게 및 혈액세포에 미치는 효과를 조사하였다 ${\gamma}$-선 조사 24시간 전에 Gu 추출물을 복강 투여하였을시, ${\gamma}$-선을 조사한 생쥐의 40-day 생존율을 65.5%에서 78.6%로 증가시켰다. Gu 추출물의 투여는 ${\gamma}$-선 조사에 의한 비장과 흉선의 무게 감소를 완전히 막아주었다(P<0.05, P<0.01) 또한 이와 유사하나 다소 적은 방사선 방호효과가 Gu 투여군의 고환에서도 관찰되었다(P<0.05). Gu 추출물의 투여는 ${\gamma}$-선 조사시 나타나는 조사 후 7일째까지의 백혈구와 림프구수의 현저한 감소를 지연시켰고, 회복속도를 촉진시켰다. 또한 21일째후의 적혈구수의 회복을 촉진시켰다. 이와 같은 결과에서 Gu 추출물의 방사선 방호효과를 알 수 있다.

• 한국수의병리학회:학술대회논문집
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• 한국수의병리학회 2001년도 추계학술대회 및 정기총회
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• pp.22-22
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• 2001

We performed this study to determine the effect of Panax ginseng and its fractions on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells of mice irradiated with high and low dose of $\gamma$-irradiation. The radioprotective effect of ginseng was compared with the effect of diethyldithiocarbamate (DDC). Ginseng administration before irradiation protected the jejunal crypts (p<0.005), increased the formation of endogenous spleen colony (p<0.005) and reduced the frequency of radiation-induced apoptosis (p<0.005). (omitted)