• Journal of Ecology and Environment
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• v.43 no.2
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• pp.254-262
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• 2019

Background: To exploit the ornamental and medicinal purposes of Lonicera harae Makino, L. subsessilis Rehder, L. praeflorens Batalin, and L. insularis Nakai, native to Korea, it is necessary to understand their seed ecology for propagation. In this study, we investigated the seed dormancy type and germination characteristics of seeds of the four Korean native Lonicera species. Results: The seeds of the four Lonicera species imbibed water readily, suggesting that the species do not have physical dormancy. Furthermore, the seeds exhibited underdeveloped embryos with only about 15-25% of the length of the seeds at dispersal. The embryos grew to the critical length with approximately 50-80% of the length of the seeds' development before radicle protrusion. Further, 94.4% and 61.1% of freshly matured seeds of L. insularis and L. harae germinated within 4 weeks after sowing at 15 ℃ and 20 ℃, respectively. Contrarily, L. praeflorens and L. subsessilis seeds did not germinate within 4 weeks under all temperature treatments. At 15 ℃, L. praeflorens seeds started to germinate from 5 weeks and the final germination rate was 51.1% at 13 weeks. At 15 ℃, L. subsessilis seeds started to germinate from 5 weeks after sowing and the final germination rate was 85.6% at 17 weeks after sowing. Embryo growth and germination of L. praeflorens and L. subsessilis occurred at a relatively high temperature (≥ 15 ℃). Conclusions: Overall, L. insularis seeds have only morphological dormancy. The seeds of L. harae have approximately 60% and 40% of morphological dormancy and morphophysiological dormancy, respectively. Contrarily, L. praeflorens and L. subsessilis exhibited non-deep simple-type morphophysiological dormancy that requires relatively high temperature (≥ 15 ℃) for embryo growth and dormancy breaking. The optimum temperature for the germination of seeds of L. insularis, L. harae, L. praeflorens, and L. subsessilis was 15 ℃, 20 ℃, 15 ℃, and 20 ℃, respectively. There was interspecific variation in seed dormancy and germination patterns in the four Lonicera species. The difference in these characteristics within the four Lonicera species could be useful for understanding the seed ecophysiological mechanisms of Lonicera species.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.42 no.4
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• pp.373-377
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• 1997

The bird repellent, methiocarb 50% WP, has been used to reduce the bird damage in the crop field in the European countries. The bird damage occaisonally would occur in the wet direct seeded rice and in soybean field, and resulted in decreasing the crop productivity by the reduction of seedling emergence rate. In this experiment, rice seeds, Hwasungbyeo(Oryza sativa), were coated with the different application rate of methiocarb, 5, 10 and 15 per kg seed, and soybean, Taegwangkong(Glycine max), 2.5, 5.0 and 7.5g. In rice, the seeds coated with 10 and 15g of methiocarb were not lost by bird, while those with 5g and control were lost to 37 and 50% of total seeds, respectively. No damage by birds was observed in rice seedling when the coleoptile and radicle of rice were emerged at 7 days after the water seeding. The crop injury of methiocarb reducing the emergence rate of seedlings, shortening the shoot length, and decreasing the leaf number was occurred at the treated of methiocarb 15g per kg seed. In soybean, the loss of the sprouting by birds was lower in the treatment of methiocarb 7.5g per kg seed than that in both the control and the treatments of methiocarb, 2.5 and 5.0g per kg seed. No crop injury by the treatment of methiocarb was observed in all application rate.

• Horticultural Science & Technology
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• v.34 no.2
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• pp.236-241
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• 2016

The common ice plant (Mesembryanthemum crystallinum L.) has some medicinal uses and recommended plant in closed-type plant factory. The objective of this study was to estimate the cardinal temperatures for seed germination of the common ice plant using bilinear, parabolic, and beta distribution models. Seeds of the common ice plant were germinated in the dark in a growth chamber at four constant temperatures: 16, 20, 24, and $28^{\circ}C$. For this, four replicates of 100 seeds were placed on two layers of filter paper in a 9-cm petri dish and radicle emergence of 0.1 mm was scored as germination. The times to 50% germination were 4.3, 2.5, 2.0, and 1.8 days at 16, 20, 24, and $28^{\circ}C$, respectively, indicating that the germination of this warm-weather crop increased with temperature. Next, the time course of germination was modeled using a logistic function. For the selection of an accurate model, seeds were germinated in the dark at constant temperatures of 6, 12, 32, and $36^{\circ}C$. Germination started earlier and increased rapidly at temperatures above $20^{\circ}C$. The minimum, optimal, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate, as a function of the temperature gradient. The different functions estimated differing minimum, optimal and maximum temperatures, with 5.7, 27.7, and $36.5^{\circ}C$, respectively for the bilinear function, 13.4, 25.0, and $36.6^{\circ}C$, respectively, for the parabolic function and 7.8, 25.9, and $36.0^{\circ}C$, respectively, for the beta distribution function. The models estimated that the inverse of time to 50% germination rate was 0 at 6 and $36^{\circ}C$. The observed final germination rates at 12 and $32^{\circ}C$ were 62 and 97%, respectively. Our data show that a beta distribution function provides a useful model for estimating the cardinal temperatures for germination of seed from the common ice plant.

• Current Research on Agriculture and Life Sciences
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• v.7
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• pp.41-46
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• 1989

The seeds of Malus sieboldii were collected in late fall of 1988 and stored in cold stratification condition for 15 weeks. Growth regulators such as $GA_3$, BA and ABA were treated to the seed to know germination capacity and seedling growth. The results were as follows. 1) Higher germination was achieved in $GA_3$ and BA plots, But ABA treatment showed lower germination percentage and physiological dwarf and also induced to the secondary dormancy condition in partly. 2) Average length of time for germination was delayed over 2 days in ABA plot when comparing with the other plots. 3) Average length of plumule and radicle also was inhibited by ABA treatment. This means lower seedling vigor. 4) Fresh and dry weight of the seeding were higher in BA plot while ABA plot was lower and undesirable seedling was produced. From above mentioned results, it is believed that ABA treatment to the seed induced the secondary dormancy and physiological dwarf pattern. Therefore, utilization of BA can be produced the normal seedling, the authors believed.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.41 no.4
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• pp.482-488
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• 1996

The experiment was carried out to the find variation of the sugar contents in the tip and basal part of the cotyledon and remaining portion of embryos in Phaseolus vulgahs seeds during germination with HPLC analysis method. Water content in cotyledon of kidney bean seed was about 6.4~6.5 of fresh weight and incresed to 45.8~71.2% during germination showing that tip part of cotyledon has more water content by 1.1~3.8% compared to the lower part of it. Higher water onten was observed in the rest parts of the seed except cotyledon such as plumule, radicle and hypocotyl showing that it increased to 72.2~93.3% depending on the different tissue organs. Main important sugars in kidney seeds during germination stages are; raffinose, sucrose, glucose and fructose, and the amount are differed with the kinds of embryo in kidney bean seed organs and stages of germination. Raffinose amount in kidney bean is increasing repeatly when seeds become wet but disappear it soon after seed have germinated especially in growing embryo parts. Raffinose in basal parts of cotyledons were still presented some an amount after germination. Sucrose is synthesized highly in plumule embryo at the beginning stage of germination but disappeared it from 5 days after seeding stages. Amount of sucrose in cotyledon of kidney seeds and seedlings increased continuously after germination. The amount of glucose and fructose in the cotyledons of kidney seeds during germination varied 5~10% or 5~15% but in the germinating and growing organs, plumule, they increased continuously after germination.

• The Korean Journal of Pesticide Science
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• v.19 no.4
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• pp.399-401
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• 2015

This study investigated the plant growth promoting activity of geraniol on Chinese cabbage (Brasscia campestris ssp. Pekinensis Rupr.), Pak Choi (Brassica rapa L.), Crown daisy (Chrysanthemum coronarium L.), Iceberg lettuce (Lactuca sativa L.) and Leaf perilla (Perilla frutescens Britt.). Geraniol at 0.5 to $10mg\;L^{-1}$ promoted radical growth of Chinese cabbage Pak Choi, Crown daisy, Iceberg lettuce and leaf perilla by >30%. In the pot experiments, geraniol treatment at $20mg\;L^{-1}$ by drenching increased up to 190% of the aerial part growth promotion. By comparison, foliar application showed 124% promoting activity only at $100mg\;L^{-1}$.

• Food Science and Preservation
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• v.22 no.4
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• pp.591-597
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• 2015

In this study, extracts of chlorella using different extraction methods were compared for antioxidant activities and spray-dried chlorella powders were investigated for their physicochemical characteristics. The DPPH radical scavenging activity and superoxide radical scavenging activity of 50% ethanol extract were 29.19%, and 48.91%, respectively. The oxygen radical absorbance capacity (ORAC) of the 50% ethanol extract ($150.44{\mu}M/g$) was higher than those of other extracts. The total chlorophyll content of the 50% ethanol extract (542.89 mg/100 g) was higher than those of other extracts. The microencapsulation of the 50% ethanol extract was manufactured by spray-drying with 10 % maltodextrin (SD-C10), 20% maltodextrin (SD-C20), and 30% maltodextrin (SD-C30). The particle size of the freeze-dried powder ($454.47{\mu}m$) was higher than those of the spray-dried powders ($24.15{\sim}32.49{\mu}m$). Scanning electron microscope images showed that the spray-dried chlorella powders using SD-C10, SD-C20, and SD-C30 had an uniform particle distribution. The water absorption index and water solubility index (WSI) of powders were 0.31~0.45, and 96.96~98.28%, respectively. The spray-dried powders showed the stability in total chlorophyll content for 40 days storage. Based on these results, spray-dried chlorella powders could be used in various types of food processes.

• The Korean Journal of Ecology
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• v.28 no.3
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• pp.135-139
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• 2005

This study was carried out to find the allelopathic effect of volatile materials released from Eupatorium rugosum. The GC method was employed for analysis of volatile materials from E. rugosum and 49 chemical substances were identified such as $\beta$-caryophyllene, $\alpha$-terpinenol, chamazulene, bornyl acetate, $\alpha$-pinene, etc. including unidentified three chemicals. Germination test in Phaseolus radiatus was done to find the inhibition effect of volatile materials using some chemicals which were proved to be important component or much amounts ones in E. rugosum. It was strongly inhibited by linalool and terpinen-4-ol. Seedling elongation and radicle growth of that were proportionally inhibited by the concentration of the essential oil, especially $\alpha$-pinene and bornyl acetate. Biomass of receptor plant was slightly decreased more than 58 ${\mu}l$ of the extract in case of $\alpha$-pinene, while it was decreased more than 19 ${\mu}l$ of that in bornyl acetate but it was shown non-significant. From the above results, it was found that volatile materials from E. rugosum showed a allelopathic effect and also $\alpha$-pinene, bornyl acetate, linalool and terpinen-4-ol used in bioassay were some of major allelochemicals in germination inhibition and especially linalool and terpinen-4-ol are prominent effect on growth inhibition of other plant.

• Journal of Korean Society of Forest Science
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• v.99 no.6
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• pp.871-877
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• 2010

This study was conducted to investigate the effects of host tree species, temperature and humidity on ex vitro for seed germination in endangered species of Loranthus tanakae. In addition, we compared seed shapes between Loranthus tanakae and Viscum album that we could have easily shown in ex vitro condition. Seeds were germinated after one week inoculation and followed to develop radicles. Seed germination rates of Loranthus tanakae were 80~95% in most of the experimental conditions. The highest rate of holdfast penetrated to host plants was 72% in Populus alba var. pyramidalis among 13 different species tested at $20^{\circ}C$. Also the rates of their penetration were 57% in Morus bombycis, 55% in Acer palmatum and 42% in Castanea crenata at $20^{\circ}C$. Seeds were germinated under condition without irrigation and followed to withered in 12 weeks later. Stages of seed germination of the Loranthus tanakae were followed by radicle induction, holdfast development, haustorium formation and penetration in order in total period of 14 weeks.

• Journal of Bio-Environment Control
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• v.23 no.1
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• pp.39-42
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• 2014

The objective of this study was to estimate cardinal temperatures for germination of lettuce (Lactuca sativar L.) using bilinear, parabolic, and beta distribution functions. Seeds of lettuce were germinated in a growth chamber at 7 constant temperatures: 10, 14, 16, 20, 24, 28, and $32^{\circ}C$. Four replicates of 100 seeds were placed on two layers of filter paper in a 9 cm petri-dish. Radicle emergence of 1 mm was scored as germination. The time course of germination was modeled using a logistic function. These minimum, optimum, and maximum temperatures were estimated by regression of the inverse of time to 50% germination rate against the temperature gradient. In bilinear function, minimum, optimum, and maximum temperatures were $7.9^{\circ}C$, $23.3^{\circ}C$, and $28.0^{\circ}C$, respectively. In parabolic function, minimum, optimum, and maximum temperatures were $9.7^{\circ}C$, $19.5^{\circ}C$, and $29.4^{\circ}C$, respectively. In beta distribution function, minimum, optimum, and maximum temperatures were $3.7^{\circ}C$, $20.7^{\circ}C$ and $32.0^{\circ}C$, respectively. Minimum, optimum, and maximum ranges of temperatures were $3.7{\sim}9.7^{\circ}C$, $19.5{\sim}23.3^{\circ}C$, and $28.0{\sim}32.0^{\circ}C$, respectively.