• 제목/요약/키워드: Radiation-tolerant

검색결과 43건 처리시간 0.021초

방사선 환경내에서의 CCTV 카메라 개발 (Development of CCTV Camera in Radiation Environment)

  • 소수길;이용범;최영수;김성구;변의교;유승욱;하달규
    • 전력전자학회:학술대회논문집
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    • 전력전자학회 1999년도 전력전자학술대회 논문집
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    • pp.403-406
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    • 1999
  • For a man's approaching limitation in radiation environment, all work must be performed with remoted system to ensure worker's safety and reliability from radiation damage. The remoted system is mostly used in visual observation CCTV system. In high radiation environment of unclear power plant, RI(Radio-isotope) facility, medical radiation treatment facility must be used to radiation tolerant CCTV cameras for radiation damage. We have studied a radiation basic performance of camera components and CCTV cameras to develop radiation tolerant CCTV cameras. As a result, we are able to design a radiation tolerant camera of 108 rad total dose.

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Selection and Genetic Relationship of Salt Tolerant Rice Mutants by in vitro Mutagenesis

  • Song, Jae Young;Kim, Dong Sub;Lee, Myung-Chul;Lee, Kyung Jun;Kim, Jin-Baek;Kim, Sang Hoon;Yun, Song Joong;Kang, Si-Yong
    • 방사선산업학회지
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    • 제4권4호
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    • pp.307-312
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    • 2010
  • Plants have evolved physiological, biochemical and metabolic mechanisms to increase their survival under the adverse conditions. This present study has been performed to select salt-tolerant rice mutant lines through in vivo and in vitro mutagenesis with gamma-rays. For the selection of the salt-tolerant rice mutants, we conducted three times of selection procedure using 1,500 gamma ray mutant lines resulted from an embryo culture of the original rice cv. Dongan (wild-type, WT): first, selection in the a nutrient solution with 171 mM NaCl; second, selection under in vitro condition with 171 mM NaCl; and third, selection in a reclaimed saline land. Based on a growth comparison of the entries, out of the mutant lines, two putative 2 salt tolerant (ST) rice mutant lines, ST-87 and ST-301, were finally selected. The survival rate of the WT, ST-87 and ST-301 were 36.6%, 60% and 66.3% after 7 days in 171 mM NaCl treatment, respectively. The WT and two salt tolerant mutant lines were used to analyze their genetic variations. A total of 21 EcoRI and Msel primer combinations were used to analyze the genetic relationship of among the two salt-tolerant lines and the WT using the ABI3130 capillary electrophoresis system. In the AFLP analysis, a total of 1469 bands were produced by the 21 primer combinations, and 700 (47.6%) of them were identified as having polymorphism. The genetic similarity coefficients were ranged from 0.52 between the ST-87 and WT to 0.24 between the ST-301 and the WT. These rice mutant lines will be used as a control plot for physiological analysis and genetic research on salt tolerance.

Protein Profiles in Response to Salt Stress in Seedling of Salt Tolerant Rice Mutants

  • Song, Jae Young;Kim, Dong Sub;Lee, Myung-Chul;Lee, Kyung Jun;Kim, Jin-Baek;Kim, Sang Hoon;Ha, Bo-Keun;Lee, Young-Keun;Kang, Si-Yong
    • 방사선산업학회지
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    • 제6권2호
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    • pp.129-138
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    • 2012
  • Proteomic analysis was performed in order to identify proteomic changes by salt stress between the Japonica cv. Donganbyeo (WT) and two salt-tolerant (ST) mutant lines by using the SDS-PAGE and 2-DE. Two salt tolerant rice mutant lines, ST-87 and ST-301, were selected by in vitro mutagenesis with gamma-ray. Three-week-old seedlings were treated with 171 mM NaCl for 7 days. In the SDS-PAGE, three proteins with molecular weights of 27, 46 and 58 kDa were highly increased under salt treatment. Total proteins from shoots of both WT and ST-lines were separated by two-dimensional gel electrophoresis. In 2-DE, 201, 226, 217 and 213 protein spots were detected in the untreated-or treated-WT and untreated- or treated-ST-87, respectively. Of theses, 17 and 10 protein spots were up- and down-regulated under salt stress in the WT, respectively. While, 16 and 8 protein spots were up- and down-regulated under salt stress in the ST-87, respectively, compared with the untreated plants. High intensity or de novo synthesized proteins were analyzed by MALDI-TOF/MS analysis.

I 형 게이트 내방사선 n-MOSFET 구조 설계 및 특성분석 (Design of a radiation-tolerant I-gate n-MOSFET structure and analysis of its characteristic)

  • 이민웅;조성익;이남호;정상훈;김성미
    • 한국정보통신학회논문지
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    • 제20권10호
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    • pp.1927-1934
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    • 2016
  • 본 논문에서는 일반적인 실리콘 기반 n-MOSFET(n-type Metal Oxide Semiconductor Field Effect Transistor)의 절연 산화막 계면에서 방사선으로부터 유발되는 누설전류 경로를 차단하기 위하여 I형 게이트 n-MOSEFT 구조를 제안하였다. I형 게이트 n-MOSFET 구조는 상용 0.18um CMOS(Complementary Metal Oxide Semiconductor) 공정에서 레이아웃 변형 기법을 이용하여 설계되었으며, ELT(Enclosed Layout Transistor)와 DGA(Dummy Gate-Assisted) n-MOSFET와 같은 레이아웃 변형 기법을 사용한 기존 내방사선 전자소자의 구조적 단점을 개선하였다. 따라서, 기존 구조와 비교하여 반도체 칩 제작에서 회로 설계의 확장성을 확보할 수 있다. 또한, 내방사선 특성 검증을 위하여 TCAD 3D(Technology Computer Aided Design 3-dimension) tool을 사용하여 모델링과 모의실험을 수행하였고, 그 결과 I형 게이트 n-MOSFET 구조의 내방사선 특성을 확인하였다.

Radiation tolerant capacitor-SRAM without area overhead

  • Eunju Jo;Hosang Yoon;Hongjoon Park;Woo-young Choi;Inyong Kwon
    • Nuclear Engineering and Technology
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    • 제56권8호
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    • pp.2916-2922
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    • 2024
  • \\In memory semiconductors such as a static random access memory (SRAM), a common problem is soft errors under radiation environment. These soft errors cause bit flips, which are referred to as single event upsets (SEUs). Some radiation-hardened SRAM cells such as a Quatro SRAM, we-Quatro SRAM, and DICE SRAM cells have been reported for years. However, these designs have the disadvantage of taking up more area than a conventional 6T SRAM cell. Thus, we propose a radiation-hardened SRAM cell design that we named capacitor-static random access memory (C-SRAM) without area overhead. The C-SRAM is formed by simply adding a capacitor to the conventional 6T SRAM. It was designed to mitigate the radiation effect using the conservation law of electrical charge. Moreover, it has the same cell size as the conventional 6T SRAM cell. Its static noise margins (SNMs), which are indicators of operational stability, are equal to the conventional 6T SRAM values of 530 mV, 220 mV, and 860 mV in hold, read, and write modes, respectively. The results of the SEU simulation test showed that it had 4.761 times better flipping tolerance than the conventional 6T SRAM with a charge value of 247.494 fC. In addition, irradiation experiments also confirmed that the C-SRAM cell was more tolerant than the 6T SRAM cell. The conventional 6T SRAM and C-SRAM were fabricated using a standard 0.18 ㎛ CMOS process.

Ultraviolet-B radiation sensitivities in rice plant: cyclobutane pyrimidine dimer photolyase activities and gene mutations

  • Hidema, Jun;Kumagai, Tadashi
    • 한국식물생명공학회:학술대회논문집
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    • 한국식물생명공학회 2004년도 생명공학 실용화를 위한 비젼
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    • pp.29-34
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    • 2004
  • Reduction in stratospheric ozone layer increases the amount of ultraviolet-B radiation (UVB: 280-320 nm) that reaches the earth ’ s surface. UVB radiationcan damage plants, resulting in decrease in growth and productivity. UVB-augmentation studies have indicated that the sensitivity to UVB radiation in plants varies among the species and cultivars. However. there are no definitive answers for the mechanisms of UVB-resistance in higher plants and for bioengineering design and development of UVB-tolerant plants. We have been studying physiological and biochemical aspects of the effects of UVB radiation on growth and yield of rice COryza sativa LJ. aiming to clarify the mechanism of resistance to UVB radiationin rice. At this meeting. weintroduce our research as followed: (1) supplementary UVB radiation has inhibitory effects on the growth. yield and grain development of rice; (2) UVB sensitivity of rice varies widely among cultivars; (3) among Japanese rice cultivars. Sasanishiki. a leading variety in northeast Japan. is more resistant to UVB. while Norin 1. a progenitor of Sasanishiki. is less resistant; (4)UV-sensitive Norin 1 cultivar is deficient in photorepair of UVB-induced cyclobutane pyrimidine dimer (CPD). and this deficiency results from one amino acid residue alteration of CPD photolyase. These results suggest that spontaneously occurring mutation in CPD photolyase gene could lead to difference in UVB sensitivity in rice. and that CPD photolyase might be a useful target for improving UVB-sensitivity in rice by selective breeding or bioengineering of UVB-tolerant rice.

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방사선을 이용한 내염성 계통의 기내선발 및 특징 (In Vitro Selection and Characterizations of Gamma Radiation-Induced Salt Tolerant Lines in Rice)

  • 이인석;김동섭;현도윤;임용표;이영일
    • Journal of Plant Biotechnology
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    • 제29권4호
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    • pp.247-252
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    • 2002
  • 1.5% NaCl이 함유된 배지에 캘러스를 치상하여 방사선을 처리한 결과 무처리구보다 처리구 (30, 50 Gy)에서 캘러스 생존율 및 재분화율이 증가하여 내염성 캘러스를 선발하는데 방사선의 적용이 효과적임을 알 수 있었고, 내염성 캘러스에서 재분화된 M$_3$세대 종자에서 내염성 계통들은 모품종보다 초장, 근장 및 근수의 생육이 우수하여 이러한 계통은 내염성 연구를 위한 유용한 유전자원으로 이용할 수 있을 것으로 생각된다. 또한 RAPD 기술은 대조구와 내염성 캘러스에서 재분화된 계통을 구분하는데 유용한 기술임을 알 수 있었다.

Isolation of Gamma-Induced Rice Mutants with Increased Tolerance to Salt by Anther Culture

  • Lee, In-Sok;Kim, Dong-Sub;Hyun, Do-Yoon;Lee, Sang-Jae;Song, Hi-Sup;Lim, Yong-Pyo;Lee, Young-Il
    • Journal of Plant Biotechnology
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    • 제5권1호
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    • pp.51-57
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    • 2003
  • Doubled haploids have long been recognized as a valuable tool in plant breeding since it not only offers the quickest method of advancing heterozygous breeding lines to homozygosity, but also increased the selection efficiency over conventional procedures due to better discrimination between genotypes within any one generation. Salt tolerant mutants were obtained in rice the variety, 'Hawsungbyeo', through in vitro mutagenesis of in vitro cultured anther-derived calli. Various doses (30, 50, 70 and 90 Gy) of gamma ray were applied to investigate the effect of radiation on callus formation on medium containing 1% NaCl, green plant regeneration, frequency of selected doubled haploid mutants and of the salt tolerant screen. It was demonstrated that the dose of 30 and 50 Gy gamma rays had significant effects on callus formation, regeneration and selection of salt tolerance. No tolerant lines were obtained from non-mutagenized cultures. From gamma ray irradiated cultures, five tolerant lines ($M_2$generation) at germination stage and 13 tolerant lines ($M_3$genoration) at seedling stage were obtained. The frequency of salt tolerant mutants indicates that anther culture applied in connection with gamma rays is an effective way to improve salt tolerance.