• Journal of the Korean Society of Marine Environment & Safety
• /
• v.20 no.6
• /
• pp.685-691
• /
• 2014

The result of probabilistic maritime risk evaluation is represented by the probability(P=0.0~1.0) These results are shown by an index using Risk Acceptance Criteria(RAC) to base the evaluation generally to know the risk level easily. Current RAC is divided into 3 steps, 5 steps, 7 steps, etc. Despite need to evaluate whether the number of RAC for risk evaluation is reasonable, there is not a related research yet. In this study, It was proposed the evaluation method to determine the optimum index number of RAC using the Sensitivity distribution characteristics and the Sensitivity by the index number of RAC. As application result from the proposed method for probabilistic risk evaluation data obtained from the prior studies, It could be determined the optimum index number of RAC by Sensitivity below 10 times and confirmed that the proposed method is reasonable by this study.

• The Korean Journal of Physiology and Pharmacology
• /
• v.27 no.3
• /
• pp.257-265
• /
• 2023

Kidney ischemia/reperfusion (I/R) injury, a common cause of acute kidney injury (AKI), is associated with the migration of inflammatory cells into the kidney. Ras-related C3 botulinum toxin substrate 1 (Rac1), a member of the Rho family of small GTPase, plays an important role in inflammatory cell migration by cytoskeleton rearrangement. Here, we investigated the role of Rac1 on kidney I/R injury and macrophage migration. Male mice were subjected to either 25 min of bilateral ischemia followed by reperfusion (I/R) or a sham operation. Some mice were administrated with either NSC23766, an inhibitor of Rac1, or 0.9% NaCl (vehicle). Kidney damage and Rac1 activity and expression were measured. The migration and lamellipodia formation of RAW264.7 cells, mouse monocyte/macrophage, induced by monocyte chemoattractant protein-1 (MCP-1, a chemokine) were determined using transwell migration assay and phalloidin staining, respectively. In sham-operated kidneys, Rac1 was expressed in tubular cells and interstitial cells. In I/R-injured kidneys, Rac1 expression was decreased in tubule cells in correlation with the damage of tubular cells, whereas Rac1 expression increased in the interstitium in correlation with an increased population of F4/80 cells, monocytes/macrophages. I/R increased Rac1 activity without changing total Rac1 expression in the whole kidney lysates. NSC23766 administration blocked Rac1 activation and protected the kidney against I/R-induced kidney damage and interstitial F4/80 cell increase. NSC23766 suppressed monocyte MCP-1-induced lamellipodia and filopodia formation and migration of RAW 264.7 cells. These results indicate Rac1 inhibition protects the kidney against I/R via inhibition of monocytes/macrophages migration into the kidney.

• Structural Engineering and Mechanics
• /
• v.64 no.4
• /
• pp.461-472
• /
• 2017

The dynamic tests of recycled aggregate concrete (RAC) are carried out, the rate-dependent mechanical models of RAC are proposed. The dynamic mechanical behaviors of RAC frame structure are investigated by adopting the numerical simulation method of the finite element. It is indicated that the lateral stiffness and the hysteresis loops of RAC frame structure obtained from the numerical simulation agree well with the test results, more so for the numerical simulation which is considered the strain rate effect than for the numerical simulation with strain rate excluded. The natural vibration frequency and the lateral stiffness increase with the increase of the strain rate. The dynamic model of the lateral stiffness is proposed, which is reasonably applied to describe the effect of the strain rate on the lateral stiffness of RAC frame structure. The effect of the strain rate on the structural deformation and capacity of RAC is analyzed. The analyses show that the inter-story drift decreases with the increase of the strain rate. However, with the increasing strain rate, the structural capacity increases. The dynamic models of the base shear coefficient and the overturning moment of RAC frame structure are developed. The dynamic models are important and can be used to evaluate the strength deterioration of RAC structure under dynamic loading.

• BMB Reports
• /
• v.46 no.12
• /
• pp.617-622
• /
• 2013

Rac1 plays a key role in neurite outgrowth via reorganization of the actin cytoskeleton. The molecular mechanisms underlying Rac1-mediated actin dynamics in the cytosol and plasma membrane have been intensively studied, but the nuclear function of Rac1 in neurite outgrowth has not yet been addressed. Using subcellular fractionation and immunocytochemistry, we sought to explore the role of nuclear Rac1 in neurite outgrowth. bFGF, a strong agonist for neurite outgrowth in PC12 cells, stimulated the nuclear accumulation of an active form of Rac1. Rac1-PBR (Q) mutant, in which six basic residues in the polybasic region at the C-terminus were replaced by glutamine, didn't accumulate in the nucleus. In comparison with control cells, cells expressing this mutant form of Rac1 displayed a marked defect in extending neurites that was concomitant with reduced expression of MAP2 and MEK-1. These results suggest that Rac1 translocation to the nucleus functionally correlates with bFGF-induced neurite outgrowth.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.7
• /
• pp.3061-3065
• /
• 2015

Background: Rac3, a member of the Rac family of small guanosine triphosphatases (GTPases), regulates a variety of cell functions, including the organization of the cytoskeleton, cell migration, and invasion. Overexpression of Rac3 has been reported in several human cancers. However, the role of Rac3 in lung cancer (LC) has not been determined in detail. The purpose of this study was to investigate the effect of silencing of Rac3 expression in human LC cells and the consequences for cell survival. Materials and Methods: Lentivirus small hairpin RNA (shRNA) interference techniques were utilized to knock down the Rac3 gene. Gene and protein expression was quantified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting. LC cell apoptosis was examined by annexin V-APC /propidium iodide staining. Results: Efficient silencing of Rac3 strongly inhibited A549 cell proliferation and colony formation ability, and significantly decreased tumor growth. Moreover, flow cytometry analysis showed that knockdown of Rac3 led to G2/M phase cell cycle arrest as well as an excess accumulation of cells in the G1 and S phase. Conclusions: Thus, functional analysis using shRNAs revealed a critical role for Rac3 in the tumor growth of LC cells. shRNA silencing of Rac3 could provide an effective strategy to treat LC.

• Molecules and Cells
• /
• v.25 no.1
• /
• pp.131-137
• /
• 2008

Crk-associated substrate (CAS) is a focal adhesion protein that is involved in integrin signaling and cell migration. CAS deficiency reduces the migration and spreading of cells, both of which are processes mediated by Rac activation. We examined the functions of v-Crk, the oncogene product of the CT10 virus p47gag-crk, which affects cell migration and spreading, membrane ruffling, and Rac activation in CAS-deficient mouse embryonic fibroblasts (CAS-/- MEFs). CAS-/- MEFs showed less spreading than did CAS+/+ MEFs, but spreading was recovered in mutant cells that expressed v-Crk (CAS-/-v-Crk MEF). We observed that the reduction in spreading was linked to the formation of membrane ruffles, which were accompanied by Rac activation. In CAS-/- MEFs, Rac activity was significantly reduced, and Rac was not localized to the membrane. In contrast, Rac was active and localized to the membrane in CAS-/-v-Crk MEFs. Lamellipodia protrusion and ruffle retraction velocities were both reduced in CAS-/- MEFs, but not in CAS-/-v-Crk MEFs. We also found that microinjection of anti-gag antibodies inhibited the migration of CAS-/-v-Crk MEFs. These findings indicate that v-Crk controls cell migration and membrane dynamics by activating Rac in CAS-deficient MEFs.

• Journal of Marine Life Science
• /
• v.2 no.2
• /
• pp.70-74
• /
• 2017

Interleukin-1 (IL-1) is one of the proinflammatory cytokines, after IL-1 binds to IL-1RI, IL-1RacP (interleukin-1 receptor accessory protein) joins with IL-1/IL-1RI to form a complex, and leading to cell activation. IL-1RAcP is involved in immune response, stress and apoptosis. The purpose of this study was to investigate the gene expression of IL-1RAcP in red seabream (Pagrus major) exposure to low water temperature (8℃, 33 psu) and low salinity (20℃, 10 psu). Results showed that, the expression of IL-1RAcP was significantly increased in the experiment groups, such as low water temperature (8℃, 33 psu), and low salinity (20℃, 10 psu). These results suggest that IL-1RAcP was played roles in biomarker gene on the environmental stress such as low water temperature and low salinity.

• Proceedings of the KSME Conference
• /
• 2007.05b
• /
• pp.3092-3097
• /
• 2007

Although noise of a RAC can be reduced effectively by decreasing RPM, condensation problems can occur to reduce reliability of the RAC for low RPM. Thus, this research has been performed to propose a design guideline of the RAC for low-noise RPM with high reliability. The internal and external flows of the RAC have been visualized and analyzed by a PIV technique to solve the condensation problem at an outlet and impeller. Then, the design guideline has been proposed by the analyzed results and confirmed by wind-tunnel and noise tests to reduce the condensation problem. Finally the shapes of the outlet with reduced condensation problem and the impeller with low noise have been obtained in this study.

• Journal of KIISE:Computer Systems and Theory
• /
• v.30 no.9
• /
• pp.461-476
• /
• 2003

Due to the implementation ease and alleviation of memory bottleneck effect, NUMA architecture has dominated in the multiprocessor systems for the past several years. However, because the NUMA system distributes memory in each node, frequent remote memory access is a key factor of performance degradation. Therefore, efficient design of RAC(Remote Access Cache) in NUMA system is critical for performance improvement. In this paper, we suggest Multi-Grain RAC which can adaptively control the RAC line size, with respect to each application behavior Then we simulate NUMA system with multi-grain RAC using MINT, event-driven memory hierarchy simulator. and analyze the performance results. At first, with profile-based determination method, we verify the optimal RAC line size for each application and, then, we compare and analyze the performance differences among NUMA systems with normal RAC, with optimal line size RAC, and with multi-grain RAC. The simulation shows that the worst case can be always avoided and results are very close to optimal case with any combination of application and RAC format.

• International Journal of Concrete Structures and Materials
• /
• v.10 no.3
• /
• pp.307-323
• /
• 2016

The nonlinear behaviors of recycled aggregate concrete (RAC) frame structure are investigated by numerical simulation method with 3-D finite fiber elements. The dynamic characteristics and the seismic performance of the RAC frame structure are analyzed and validated with the shaking table test results. Specifically, the natural frequency and the typical responses (e.g., storey deformation, capacity curve, etc.) from Model 1 (exclusion of strain rate effect) and Model 2 (inclusion of strain rate effect) are analyzed and compared. It is revealed that Model 2 is more likely to provide a better match between the numerical simulation and the shaking table test as key attributes of seismic behaviors of the frame structure are captured by this model. For the purpose to examine how seismic behaviors of the RAC frame structure vary under different strain rates in a real seismic situation, a numerical simulation is performed by varying the strain rate. The storey displacement response and the base shear for the RAC frame structure under different strain rates are investigated and analyzed. It is implied that the structural behavior of the RAC frame structure is significantly influenced by the strain rate effect. On one hand, the storey displacements vary slightly in the trend of decreasing with the increasing strain rate. On the other hand, the base shear of the RAC frame structure under dynamic loading conditions increases with gradually increasing amplitude of the strain rate.