• Title/Summary/Keyword: RVS

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Enzyme-linked immunosorbent assay (ELISA) for the detection of RVS (Retrovirus of Salmonid) (ELISA법을 이용한 연어과 어류의 RVS 검출(Retrovirus of Salmonid) 검출)

  • Oh, Myung-Joo;Yoshimizu, Mamoru
    • Journal of fish pathology
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    • v.9 no.2
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    • pp.169-176
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    • 1996
  • An indirect double antibody enzyme-linked immunosorbent assay (ELISA) was developed for rapid detection of a new virus isolated from abnormally swimming salmonid fish, RVS (Retrovirus of salmonid). Results using brain tissue homogenates, and infected cell cultures are described. The sensitivity of the methods is $10^{2.6}$ $TCID_{50}/100{\mu}l$ of the examined cell culture fluid. The specificity was confirmed by the ELISA inhibition test and virological examinations. Viral antigen could be detected in artificially infected fish tissue homogenates. The assay will allow the diagnosis of RVS-infected fish within a day.

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The Effects of Visual Rhythmic Stimulation in Gait and Proprioception with Chronic Stroke Patients (시각리듬자극이 만성뇌졸중 환자의 보행과 고유수용감각에 미치는 영향)

  • Cho, Nam-Jeong;Lee, Dong-Yeop
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.11 no.9
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    • pp.3353-3357
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    • 2010
  • The purpose of this study is to investigate the effect of visual rhythmic stimulation in gait ability and proprioception in chronic stroke patients. Twenty-one persons after six months post stroke participated in pre and post test control. The subjects were randomly assigned to a rhythmic visual stimulation(RVS) group (n=10) and control group (n=11). Training process was practiced with exercise on thirty minutes a day, three days a week for four weeks. To find out the effect, inspected the proprioception test and gait characteristics by gait analysis. In gait characteristics, the walking speed, cadence and the TUG time were significantly different from RVS group. The proprioception were significantly different RVS and control group. This study showed that the RVS training increased better functional activity by postural adjustment and gait learning of chronic stroke patients than that of control group. And so, the RVS training of hemiplegic patients was very important to successive rehabilitation. A continuous examination of RVS training could be practical use of physical therapy with exercise.

Effects of dietary supplementation with detoxified Rhus verniciflua sap on egg production, yolk lipid and intestinal microflora in laying hens

  • An, Byoung-Ki;Kim, Je-Hun;Zheng, Lan;Moon, Byung-Hern;Lee, Kyung-Woo
    • Asian-Australasian Journal of Animal Sciences
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    • v.31 no.1
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    • pp.86-90
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    • 2018
  • Objective: This study was conducted to investigate the effects of dietary detoxified Rhus verniciflua sap (RVS) on production performance, egg quality, lipid fractions of egg yolk, liver and serum, and the profile of cecal microflora in laying hens. Methods: Two hundred 52-week-old Hy-Line Brown layers were randomly divided into 4 groups with 5 replicates per group (2 hens per cage, 5 cages per replicate) and were provided with one of 4 experimental diets containing 0%, 0.05%, 0.1%, or 0.2% RVS, for 6 weeks. Due to unequal intervals of RVS doses, the interactive matrix language procedure of the SAS program was used to correct the contrast coefficients of orthogonal polynomials. Results: There were no differences in feed intake and egg weight among the groups. Egg production increased (linearly and quadratically, p<0.05) with increasing levels of RVS. Eggshell thickness increased (linear, p<0.05) as the level of RVS in diets increased. The levels of blood cholesterol and activities of glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase were not altered by dietary treatments. Increasing level of RVS increased (linear, p<0.05) the populations of cecal lactic acid bacteria. The content of yolk cholesterol decreased (linear, p<0.05) with increasing levels of dietary RVS, although there were no significant differences in each lipid fraction of the liver. Conclusion: This study indicates that dietary RVS could improve laying performance and eggshell quality, and affect cecal lactic acid bacteria in a dose-dependent manner.

Effects of Rhus verniciflua Stokes (RVS) Extract on Diet-induced Obesity in C57BL/6 mouse (옻나무 추출물이 식이에 의한 비만유도 C57BL/6 mouse에 미치는 영향)

  • Kim, Jung-Hee;Lee, Hye-Won;Ko, Byoung-Seop;Kim, Ho-Kyoung;Jeon, Won-Kyung
    • Korean Journal of Pharmacognosy
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    • v.34 no.4 s.135
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    • pp.339-343
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    • 2003
  • We investigated the effects of Rhus verniciflua Stokes (RVS) on high fat diet- induced obesity in C57BL/6 that received ad libitum exposure to a high fat diet for 8 weeks. Male C57BL/6 mice weighing $20.1{\pm}0.95\;g$ were divided into four groups and the body weight and food intake were weighed for 8 weeks. After the administration of RVS extract, the liver, subcutaneous and retroperitoneal adipose tissue in the mice were weighed and common blood count in whole blood and biochemical parameters in plasma were measured. It was founded that body weight increase was significantly suppressed by the oral administration of RVS extract in group with high fat diet. Weight of subcutaneous adipose tissue and concentration of triglyceride in plasma from mice which were administrated RVS extract were lower than obesity control group. These results suggest that RVS extract may effect on inhibition of diet-induced obesity.

Inhibitory effect of Rhus verniciflua Stokes extract in MCF-7 human breast cancer cells (참옻 추출물의 MCF-7 인체 유방암 세포에서 증식 억제효과)

  • Kim, Min Sung;An, Won Gun;Lee, Jang Cheon
    • Herbal Formula Science
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    • v.24 no.4
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    • pp.283-288
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    • 2016
  • Objectives : The purpose of this study was to investigate the anti-cancer effects of extract of Rhus verniciflua Stokes (RVS) in human breast cancer cell lines. Methods : In cultured human breast cancer MCF-7 cells, we investigated growth inhibitory effect of RVS. MCF-7 cells were cultured with various concentrations (0, 200, 300, and 400 ug/ml) of RVS at $37^{\circ}C$ for 24 h. We performed CCK-8 assay and flow cytometry for detection of Annexin V-PI staining. Results : As a result, RVS inhibits the cell growth and induction of apoptosis in dose dependent manner in MCF-7 breast cancer cells. Conclusion : RVS has anti-cancer activities and induced apoptosis in human breast cancer MCF-7 cells. Therefore we suggest that RVS can use as a novel class of anti-cancer drugs.

Rhus Verniciflua Stokes Extract Suppresses Expression of Metalloproteinases, iNOS and COX-2 in THP-1 Cells Via Inhibiting NF-𝜅B and MAPK Phosphorylation

  • Ko, Hwanjoo;Jang, Eungyeong;Kim, Youngchul
    • The Journal of Korean Medicine
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    • v.41 no.4
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    • pp.12-26
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    • 2020
  • Objectives: The aim of this study is to investigate the mechanisms involved in the anti-inflammatory and anti-tumor effects of Rhus verniciflua Stokes (RVS) on PMA-differentiated human monocytic leukemia THP-1 cells. Methods: Cells were treated with various concentrations of RVS decoction (0-300㎍/ml) for 24, 48, and 72h. Cell viability was evaluated by MTS/PMS assay. The expressions of MMP-2, MMP-9, TIMP-1, TIMP-2, iNOS and COX-2 mRNA and proteins were measured using RT-PCR and western blotting, respectively. Results: RVS suppressed expression of MMP-2 and MMP-9 mRNA. It also down-regulated iNOS and COX-2 mRNA and protein expression. RVS inhibited NF-𝜅B p65 activity and the phosphorylation of Akt and MAPK (ERK and p38 MAPK). Instead, the phosphorylation of JNK is increased at a very low concentration but decreased at higher concentrations. Conclusion: RVS is regarded to inhibit the expression of MMP and TIMP as well as iNOS and COX-2 gene expression via directly inhibiting the activation of NF-𝜅B and phosphorylation of MAPK pathway in THP-1 cells. This suggests RVS have potential to be used as a therapeutic agent for acute myeloid leukemia (AML).

MPEG-I RVS Software Speed-up for Real-time Application (실시간 렌더링을 위한 MPEG-I RVS 가속화 기법)

  • Ahn, Heejune;Lee, Myeong-jin
    • Journal of Broadcast Engineering
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    • v.25 no.5
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    • pp.655-664
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    • 2020
  • Free viewpoint image synthesis technology is one of the important technologies in the MPEG-I (Immersive) standard. RVS (Reference View Synthesizer) developed by MPEG-I and in use in MPEG group is a DIBR (Depth Information-Based Rendering) program that generates an image at a virtual (intermediate) viewpoint from multiple viewpoints' inputs. RVS uses the mesh surface method based on computer graphics, and outperforms the pixel-based ones by 2.5dB or more compared to the previous pixel method. Even though its OpenGL version provides 10 times speed up over the non OpenGL based one, it still shows a non-real-time processing speed, i.e., 0.75 fps on the two 2k resolution input images. In this paper, we analyze the internal of RVS implementation and modify its structure, achieving 34 times speed up, therefore, real-time performance (22-26 fps), through the 3 key improvements: 1) the reuse of OpenGL buffers and texture objects 2) the parallelization of file I/O and OpenGL execution 3) the parallelization of GPU shader program and buffer transfer.

Effect of Dietary Rhus verniciflua Stokes on the Quality of Hanwoo (Korean Cattle) Beef during Cold Storage after Thawing (옻 급여가 한우육의 해동후 냉장중 품질에 미치는 영향)

  • Liang Cheng-Yun;Kang Sun-Moon;Kim Yong Sun;Lee Sung Ki
    • Food Science of Animal Resources
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    • v.25 no.2
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    • pp.196-202
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    • 2005
  • This study was carried out to investigate the effect of dietary Rhus verniciflua Stokes on the quality of Hanwoo (Korean cattle) beef during cold storage $(4^{\circ}C)$ after thawing. After 4 groups (3 heads/group) of 22 months-Hanwoo (Korean cattle) steen were fed a common basal diet with 0, 2, 4 and $6\%$ Rhus verniciflua Stokes (RVS) powder for 4 months prior to slaughter, samples of M semimembranous from 12 carcasses were stored at $-20^{\circ}C$ for 9 months. Crude fat was significantly lower in 4 and $6\%$ RVS treatments than in control (p<0.05). pH value was significantly higher in4 and $6\%$ RVS treatments than in control from 2 and 5 days (p<0.05). Thawing loss was not significantly different among the treatments but drip loss was significantly lower in 4 and $6\%$ RVS treatments than in control during storage (p<0.05). CIE $L^{\ast}$ value was significantly lower in RVS treatments than in control during storage (p<0.05) and CIE $a^{\ast}$ value was significantly higher in 4 and $6\%$ RVS treatments than in control (p<0..05). CIE $b^{\ast}$ value was significantly higher in control than in $6\%$ RVS treatment in 0 days (p<0.05) but it was significantly higher in $4\%$ RVS treatment than in control in 7 days (p<0.05). CIE $C^{\ast}$ value was significantly higher in $4\%$ RVS treatment than in control during storage (p<0.05) and CIE $h^0$ value was significantly lower in $4\%$ RVS treatment than in control (p<0.05). TBARS value of 2 days was significantly lower in 4 and $6\%$ RVS treatments than in control (p<0.05) but it was significantly lower in $4\%$ RVS treatment than the other treatments from 5 days (p<0.05). Surface MetMb concentration was significantly lower in RVS treatment than in control during storage (p<0.05) and it was significantly lower in $4\%$ RVS treatment than in the other treatments in 7days (p<0.05) but surface $R_{6.30}/R_{580}$ value was opposite to this result.

One Case Report of Non-small Cell Lung Cancer Patients Treated with Allergen Removed Rhus Verniciflua Stokes(aRVS) (알러젠 제거 옻나무 추출물 투여로 삶의 질 개선과 생존기간 연장을 보인 비소세포성 폐암 환자 1례)

  • Yu, Seung-Min;Eo, Wan-Kyu;Yoon, Seoung-Woo
    • Journal of Korean Traditional Oncology
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    • v.13 no.1
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    • pp.63-69
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    • 2008
  • Non-small cell lung cancer (NSCLC) is the leading cause of cancer-related mortality worldwide. Standard treatment such as chemotherapy, radiotherapy, and surgery have many limitations in the treatment of this disease. Therefore, new molecular-targeted therapies needed and being developed. Patients with advanced NSCLC have a short life expentancy; therefore, in addition to increasing their survival, improving their quality of life (QoL) is also an important treatment goal. In this case report, we introduce NSCLC patient treated with Allergen Removed Rhus Verniciflua Stokes(aRVS). In this case, survival time incresed as traditional korean medicine using aRVS. And the general condition of the patient became better. Further case study will be needed in order to determine the effects of aRVS on NSCLC.

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The Effects of Visual Rhythmic Stimulation in Gait and Proprioception with Chronic Stroke Patients (시각리듬자극이 만성뇌졸중 환자의 보행과 고유수용감각에 미치는 영향)

  • Cho, Nam-Jeong;Lee, Dong-Yeop
    • Proceedings of the KAIS Fall Conference
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    • 2010.05b
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    • pp.898-901
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    • 2010
  • 본 연구는 만성 뇌졸중 환자를 대상으로 시각리듬자극(RVS)을 이용한 보행 운동을 적용하여 보행과 고유수용성감각에 미치는 효과를 알아보고자 하였다. 뇌졸중으로 6개월 이상 장애를 가진 21명이 연구에 참여하였고 실험군 10명과 대조군 11명으로 나누었다. 대조군은 14m의 보행로가 확보된 공간에서 준비운도 5분, 보행운동 20분, 정리운동 5분 씩 주 3회, 4주간 12회을 실시하였고, 실험군은 대조군의 운동프로그램과 같은 조건에서 보행운동시 시각리듬자극(RVS)을 추가적으로 적용하였다. 운동 전과 후에 보행과 고유수용성감각을 측정하여 효과를 비교하였다. 통계처리 방법으로 실험 전 후 차이를 검증하기 위하여 대응표본 t 검정을 실시하였고 대조군과의 차이 검증을 위하여 독립표본 t 검정을 실시하였다. 모든 통계적 유의수준은 0.05로 하였다. 본 연구의 결과 시각리듬자극(RVS)이 적용된 실험군에서 보행속도와 분속수, TUG 시간이 유의하게 증가하였고(p<.05), 고유수용성감각이 유의하게 증가하였다(p<.05). 결론적으로 시각리듬자극(RVS)을 이용한 보행운동이 만성 뇌졸중 환자의 보행과 고유수용성감각에 효과가 있는 것으로 나타났다. 향후 만성 뇌졸중 환자에게 음악적 요소인 시각리듬자극 (RVS)이 정신적 육체적 기능을 상실한 뇌졸중 환자의 재활치료 프로그램에 적용하는 연구가 필요할 것으로 기대되어진다.

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