• Journal of Gastric Cancer
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• 제24권4호
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• pp.451-463
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• 2024

Purpose: Although laparoscopic distal gastrectomy has rapidly replaced open distal gastrectomy, laparoscopic total gastrectomy (LTG) is less frequently performed owing to technical difficulties. Robotic surgery could be an appropriate minimally invasive alternative to LTG because it alleviates the technical challenges posed by laparoscopic procedures. However, few studies have compared the oncological safety of robotic total gastrectomy (RTG) with that of LTG, especially for advanced gastric cancer (AGC). Herein, we aimed to assess the oncological outcomes of RTG for AGC and compare them with those of LTG. Materials and Methods: We retrospectively reviewed 147 and 204 patients who underwent RTG and LTG for AGC, respectively, between 2007 and 2020. Long-term outcomes were compared using inverse probability of treatment weighting (IPTW). Results: After IPTW, the 2 groups exhibited similar clinicopathological features. The 5-year overall survival was comparable between the 2 groups (88.5% [95% confidence interval {CI}, 79.4%-93.7%] after RTG and 87.3% [95% CI, 80.1%-92.0%]) after LTG; log-rank P=0.544). The hazard ratio (HR) for death after RTG compared with that after LTG was 0.73 (95% CI, 0.40-1.33; P=0.304). The 5-year relapse-free survival was also similar between the 2 groups (75.7% [95% CI, 65.2%-83.4%] after RTG and 76.4% [95% CI, 67.9%-83.0%] after LTG; log-rank P=0.850). The HR for recurrence after RTG compared with that after LTG was 0.93 (95% CI, 0.60-1.46; P=0.753). Conclusions: Our findings revealed that RTG and LTG for AGC had similar long-term outcomes. RTG is an oncologically safe alternative to LTG and has technical advantages.

• 운동과학
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• 제21권3호
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• pp.373-384
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• 2012

흡기근 훈련을 동반한 유산소운동이 흡기근력, 폐기능, 최대산소섭취량(maximal oxygen uptake; VO₂max)에 어떠한 영향을 미치는지 연구하였다. 24명의 건강한 대학생이 3 그룹으로 나뉘어 6주간의 실험에 참여하였다. 대상자들은 호흡근육훈련(RTG; n=8), 유산소성달리기훈련(REG; n=8), 호흡근육 및 달리기훈련(BTG; n=8) 그룹으로 나뉘었다. 실험전과 후 폐기능, 최대흡기압력(maximal inspiratory pressure; PImax), VO₂max가 평가되었다. RTG는 실험기간 동안 흡기근육훈련(inspiratory muscle training; IMT) 프로그램에 참여하였으며, PImax 의 50 % 강도로, 하루 2회, 주당 4일, 회당 30회 훈련하였다. REG는 개인별 VO₂max의 70-75 % 강도로, 하루 1회, 주당 4회, 회당 30분 달리기 훈련을 하였다. BTG는 같은 기간 동안 IMT와 달리기훈련 모두에 참여하였다. 실험기간 중 대상자들의 체격변인과 폐기능 변인은 변하지 않았다. VO₂max는 RTG, REG, BTG에서 각각 6.1±3.3 %, 5.9±6.6 %, 10.0±8.3 % 증가하였고(p< .05), PImax 또한 RTG, REG, BTG에서 각각 21.7±14.3 %, 19.7±12.0 %, 27.0±12.1 % 증가하였으나(p< .05), 그룹 간의 유의한 차이는 나타나지 않았다. 결론적으로, 모든 그룹에서 향상이 관찰되었으며, 흡기근 훈련과 유산소운동을 동반한 그룹에서 VO₂max와 PImax의 가장 큰 향상을 보였다.

• 대한산업공학회지
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• 제17권2호
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• pp.51-61
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• 1991

This paper presents a heuristic method for the Assembly Line Balancing(ALB) of the large-sized product. In the ALB problem of the large-sized product such as bus and truck, the assignments of the Related Task Groups(RTG), the same side tasks, and team tasks should be considered. In this paper, a new concept of the RTG and two kinds of assignment rules are proposed to resolve the above considerations. The first assignment rule allots the RTG with the constraint of the same side tasks to the station while the second allots the RTG to the station, relaxing the above constraint to increase the applicability of the method. An assignment rule for team tasks is also presented. The benefits of the method are to improve work methods, to give more job satisfaction to workers, and to allow greater flexibility in the design of assembly lines.

• 한국해양생명과학회지
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• 제8권2호
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• pp.186-189
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• 2023

Uncoupling protein 1 (UCP1) is a unique mitochondrial membranous protein expressed in brown adipose tissue (BAT) in mammals. While its expression in response to cold temperatures and adipogenic inducers is well-characterized in mammals and human infants, the molecular characterization and expression of UCP1 in fish remain unexplored. To address this gap, we analyzed UCP1 expression in response to adipogenic inducers in a fish cell line, rainbow trout gonadal cells (RTG-2), and compared it with UCP1 expression in three mammalian preadipocytes, 3T3-L1, T37i, and WT1 exposed to the Peroxisome proliferator-activated receptor gamma (PPARγ) agonists, rosiglitazone (Rosi). In mammalian preadipocytes, UCP1 protein was highly expressed by Rosi, with an induction of adipogenesis observed in a time-dependent manner. This suggests that UCP1 plays a significant role in adipogenesis in mammals. However, RTG-2 cells showed no response to adipogenic inducers and exhibited only marginal expressions of UCP1. These results imply that RTG-2 cells may lack crucial responsive mechanisms to adipogenic signals or that the adipogenic response is regulated by other mechanisms. Further studies are needed to confirm these phenomena in fish preadipocytes when an appropriate cell line is established in future research.

• 생명과학회지
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• 제12권4호
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• pp.490-495
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• 2002

병원성 미생물인 Vibrio fluvialis로부터 hemolysin을 분리정제 하였다. 즉, hemolysin의 정제는 균 배양액을 황산암모늄, ion exchange chromatography를 행하였으며 SDS-PAGE를 통해 그 단백질의 크기가 약 79kDa임을 알게되었다. 정제된 hemolysin (VFH)는 35$^{\circ}C$에서 최적활성을 나타내었고, 4$0^{\circ}C$에서는 그 활성이 감소되었다. RTG-2 어류유래 세포주에 hemolysin (VFH)의 처리로 세포독성을 측정한 결과 50$\mu\textrm{g}$의 VFH가 약 80%의 cell line을 사멸시켰다. 또한 현미경을 통한 관찰에서도 세포의 형태변화를 관찰할 수 있었다.

• 한국환경과학회지
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• 제23권8호
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• pp.1481-1493
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• 2014

In order to understand the relation between the distribution of sea surface temperature and heavy snowfall over western coast of the Korean peninsula, several numerical assessments were carried out. Numerical model used in this study is WRF, and sea surface temperature data were FNL(National Center for Environment Prediction-Final operational global analysis), RTG(Real Time Global analysis), and OSTIA(Operational Sea Surface Temperature and Sea Ice Analysis). There were produced on the basis of remote sensing data, such as a variety of satellite and in situ observation. The analysis focused on the heavy snowfall over Honam districts for 2 days from 29 December 2010. In comparison with RTG and OSTIA SST data, sensible and latent heat fluexes estimated by numerical simulation with FNL data were higher than those with RTG and OSTIA SST data, due to higher sea surface temperature of FNL. General distribution of RTG and OSTIA SST showed similar, however, fine spatial differences appear in near western coast of the peninsula. Estimated snow fall amount with OSTIA SST was occurred far from the western coast because of higher SST over sea far from coast than that near coast. On the other hand, snowfall amount near coast is larger than that over distance sea in simulation with RTG SST. The difference of snowfall amount between numerical assessment with RTG and OSTIA is induced from the fine difference of SST spatial distributions over the Yellow sea. So, the prediction accuracy of snowfall amount is strongly associated with the SST distribution not only over near coast but also over far from the western coast of the Korean peninsula.

• 한국어병학회지
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• 제16권2호
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• pp.69-73
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• 2003

In this study, GFP reporter gene was transfected into a fibroblast cell line RTG-2 using a polycationic transfection reagent (Superfect) and showed a successful expression of GFP. The transfection efficiency by Superfect was compared to the commonly used transfection method, i.e. DNA-calcium phosphate coprecipitatlon. Transfection by Superfect was more effective than calcium phosphate coprecipltation method (frequency of cell expressing orr was 11.3% and 3.5%, respectively). The optimal expression of GFP and {\beta}-galactosidase was observed when $5-6\;{\mu}{\ell}$ of Superfect per ${\mu}g$ DNA was used for transfcction, 1:5-6 ratio between DNA(${\mu}g$) and Superfect ($\mu\ell$).

• 대한화장품학회지
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• 제50권3호
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• pp.239-250
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• 2024

본 연구에서는 외부 환경요인에 의한 피부 손상을 예방하기 위해 왕실의료의 치료법 중 내복약인 다음(茶飮)에서 피부 보호 효과에 유효한 의미가 있는 매화, 인동덩굴, 국화, 대나무 복합추출물(로얄테라티가드^TM, RTG)을 통해 피부 안전성, 항산화, 보습, 및 항염증 효과를 확인하였다. HaCaT 세포에서 세포독성 시험 결과, RTG 복합추출물은 0.125, 0.25, 0.5, 1% 농도에서 세포 생존율의 변화가 없었고, 인체 첩포 시험에서도 피부 자극 지수 0.00점을 기록하여 피부 자극이 없는 성분임을 확인하였다. 항산화 효과는 497.83 ㎍ GAE/g의 폴리페놀 함유량과 DPPH radical 소거 활성 평가를 통해 확인되었으며, catalase 활성 증가를 통해 피부의 산화 스트레스 감소 효과가 입증되었다. 염증 완화 효과는 미세먼지 처리 후 각질형성세포에서의 염증 완화 및 tape stripping을 통한 피부 홍반 감소를 확인하였다. 보습 효과는 히알루론산 합성 효소(HAS)2, 3와 피부 장벽 인자 keratin1의 발현 증가 및 피부 수분 함량 증가와 피부 거칠기 감소를 통해 확인되었다. 이상의 결과로, RTG 복합추출물은 외부환경 요인으로 인한 피부 손상을 방지하는 화장품 소재로 활용될 수 있음을 시사한다.

• 한국어병학회지
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• 제11권2호
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• pp.89-96
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• 1998

해양버나바이러스(MABV)는 여러 종의 해양생물에 감염되며 숙주역이 넓다. 다양한 종의 숙주에 감염되었을 때의 MABV의 감염 특성을 규명하기 위하여, 주화세포 내에서 바이러스를 10대 계대 배양하여 in vitro로 연구했다. CHSE-214, RTG-2와 RSBK-2세포에서는 전형적인 CPE를 보이며 많은 양의 바이러스가 생산되었고, 높은 바이러스 단백질의 발현도 관찰되었다. 이에 반하여, EPC, FHM과 BF-2세포에서는 형광항체법에 의하여 바이러스단백질은 검출되었으나 CPE는 나타나지 않았다. EPC와 FHM 세포에서는 계대를 할수록 바이러스의 역가가 높아져, 바이러스의 숙주세포에의 적응이 일어난 것으로 보인다. 플라크의 크기는 CHSE-214, RTG-2와 RSBK-2세포에서 계대한 것이 다른 세포에서 계대한 것보다 커, 숙주세포의 종류에 따른 변이가 바이러스에 일어난 것으로 추측되었다. 게놈분절 A에 존재하는 VP2/NS 경계영역의 염기배열에서는 195번째의 염기에 특이적인 변이가 보였다. 숙주세포의 종류에 따라 다른 MABV의 감염특성은 자연계에서 다양한 숙주종에서 일어나는 in vivo에서의 감염특성을 반영하는 것으로 생각된다.

• 대한디지털의료영상학회논문지
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• 제10권1호
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• pp.45-50
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• 2008

Recently, MR Cholangiography used mainly bu controlling of patient's breathing. There is breathing hold techniques to get images within shopt time and gating technique adjusted to respiration cycle for high resolution image. In this study, the aim of this experiment is to know on clinical usefulness compared with PACE and RTG thchniques. This study's period is from 2006 in November to 2007 in January. A total of 21 patients investigated at MAGNETOM Sonata 1.5T (SIEMENS Erlangen) with use of 12ch body coil. MR acquisition protocol used 3D turbo spin echo coronal sequence. Scan parameters applied to potimal setting in use as gating techniques, respectively. Analysis of consuming timing evaluated with rapidness. As analysis of quantity, the common bile duct, gall bladder measured in signal intensities, then these data were calculated by signal to noise ratio and contrast to noise ratio. Qualitative analysis, experienced 2radiologists and 3 RTs were evaluated into 3groups about artifact, accuracy of lesions, sharpness of the common bile duct or gall bladder. As a result of analysis, when compared to PACE, consuming time of the RTG took less than PACE, On both CNRs and SNRs, PACE technique was slightly high values than RTG(p<0.05). Qualitative analysis' results, discrimination of lesions in the common bile duct, gall bladder get a significance level in both RTG and PACE techniques but presence's artifact of breathing and pulsation highly demonstrate in PACE techniques. In conclusion, both PACE and RTG methods at MRCP provided prominently clinical information for the common bile duct, gall bladder. If machines have not limitation with performance, induction of breathing holding also will help getting diagnistic quality.