• Proceedings of the Korea Institute of Fire Science and Engineering Conference
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• 2010.04a
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• pp.179-184
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• 2010

성능 위주 피난 설계에 있어서 요구피난시간(RSET)을 위한 Simulex 등 피난 시뮬레이션 툴이 사용하고 있는 근본적인 데이터베이스, 즉, 시설 및 군중 조건에 따른 이동 변수, 최대 비유동률(Maximum Specific Flows) 및 경계폭(Boundary Layer Width)을 살펴보고, 트래픽 방정식(Traffic Equation)을 통한 계산 사례 즉, 피난자의 이동 속도 계산 사례, 최소 피난 시간 계산 사례 및 총 유동 용량(Flow Capacity) 수식과 계산 사례를 살펴본다.

• Fire Science and Engineering
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• v.33 no.3
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• pp.84-90
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• 2019

In this study, a fire and evacuation inside an electronic equipment room in environmental energy facilities were conducted and evaluated using a numerical analysis method. In the fire simulation, the visual distance, temperature distribution, and CO concentration distribution were analyzed using FDS. Based on the results, the Pathfinder program, which is an evacuation simulation, was used to calculate the evacuation time of the occupants and derive an evacuation safety evaluation. As a result, the Available safe Egress time (ASET) of P-01 and P-05 was 203.3 and 398.6 s, respectively. For the Required safety Egress time (RSET) results, all evacuees were evacuated at all points and the safety of the evacuee was secured this simulation showed that the safety evaluation is based on the non - operation of the fire - fighting equipment to improve the safety, making it possible to secure better evacuation safety performance owing to the fire of other fire - fighting facilities.

• Journal of Periodontal and Implant Science
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• v.37 no.sup2
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• pp.339-351
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• 2007

Cytolethal distending toxin(CDT)은 세포 주기 중 G2에서 M 기로의 전환을 막아 세포의 증식을 억제할 수 있는 세균 단백 독소의 일종이다. 구강 미생물 중 유일하게 Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans)만이 이 CDT를 생성 할 수 있는 것으로 알려져 있다. A. actinomycetemcomitans는 localized aggressive periodontitis (LAP)의 원인균으로 여겨지며 비 운동성의 그람 음성 구간균이고 $37^{\circ}C$, 5% $CO_2$ 하에 성장이 왕성하다. A. actinomycetemcomitans의 CDT는 3개의 인접한 유전자인 cdtA, cdtB, cdtC에 의해 형성 되며 각각의 유전자에 대한 단백질의 기능은 아직 완전히 밝혀지지 않았다. 현재까지 연구에 의하면 cdtA는 CDT의 세포부착과 관련이 있는 것으로 여겨지며 이 유전자의 기능 이상 시 CDT의 독성 효과가 현저히 감소한다고 알려져 있다. 따라서 본 연구는 A. actinomycetemcomitans의 cdtA 유전자를 클로닝, 단백질 발현하여 향후 치주질환의 발병 과정에서 CdtA의 역할을 규명하고 질환의 예방 및 치료법에 도움을 주고자 하였다. A. actinomycetemcomitans Y4균주를 cdtA 유전자 클로닝을 위해 사용하였다. A. actinomycetemcomitans의 genomic DNA는 genomic DNA 추출 kit를 사용하여 분리하고 cdtA에 특이적인 primer를 이용하여 PCR을 통해 cdtA 유전자를 증폭하였다. 증폭된 cdtA 유전자를 T-vector에 클로닝 하였으며, 클로닝 된 cdtA 유전자는 단백질 발현을 위해 pRSET Avector에 서브클로닝 한 후 발현 균주인 BL21(DE3)를 이용하여 발현시켰다. 발현 후 Ni-NTA AP conjugate를 이용한 Western blot을 통해 pRSET-CDTA를 확인하였다.

• Fire Science and Engineering
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• v.31 no.2
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• pp.44-51
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• 2017

Accurate predictions of the activation time for smoke detectors using a fire simulation is are required to ensure the reliability of the RSET (Required Safe Egress Time) calculation in the process of PBD (Performance-Based Design). The objective of this study was to enhance the accuracy of input parameters for the numerical models of smoke detector based on the FDS. To this end, a Fire Detector Evaluator (FDE) developed in previous studies was improved. The uniformities of flow and smoke inside the FDE were improved and accurate measurements of the obscuration per meter (OPM) related to detector operation were also performed through a decrease in the forward scattering of smoke particles. The input parameters using the improved FDE showed a significant difference from the previous FDE quantitatively. In particular, a larger difference was found in a photoelectric detector compared to an ionization detector. Considering that the operating conditions of smoke detectors are affected by the detector type, combustibles, smoke particulars, and color, the database (DB) on the input parameters for various detectors and combustibles should be built to improve the reliability of PBD in future studies.

• Korean Journal of Veterinary Research
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• v.46 no.2
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• pp.127-133
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• 2006

Pasteurella multocida is a terrible veterinary pathogen that causes widespread infections in husbandry. To induce homologous and/or heterologous immunity against the infections, outer membrane protein Hs (OmpH) in the envelope of different strains of P. multocida are thought to be attractive vaccine candidates. Previously we cloned and characterized a gene for OmpH from pathogenic P. multocida (A : 3) (In Press, Korean J. Microbiol. Biotechnol. 2005, 33, December). The gene is composed of 1,047 nucleotides (nt) coding 348 amino acids (aa) with signal peptide of 20 aa. The truncated ompH, a gene without nt coding for the signal peptide, was generated using pRSET A to name "pRSET A/OmpH-F2". This truncated ompH was well expressed in Escherichia coli BL21 (DE3). Truncated OmpH was purified for induction of immunity against live pathogen of fowl cholera (P. multocida A : 3) in mice. Some $50{\mu}g$ of the purified polypeptide was intraperitoneally injected into mice two times with 10 day interval. Lethal dose ($25{\mu}l$) of live P. multocida A : 3 was determined by directly injecting the pathogen into wild mice (n = 25). To demonstrate the vaccine candidate of the truncated OmpH, the live pathogen ($25{\mu}l$) was challenged with the OmpH-immunized mouse group as well as positive & negative controls (n = 80). The results show that the truncated OmpH can be used for an effective vaccine production to prevent fowl cholera caused by pathogenic P. multocida (A : 3).

• Fire Science and Engineering
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• v.34 no.4
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• pp.36-44
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• 2020

It is necessary to predict the activation time of fire detectors accurately to improve the reliability for evaluating the required safe egress time (RSET) in performance-based fire safety design. In this study, problems of the plunge test, which is widely applied in assessing fire detectors, were examined through experiments and numerical simulations. In addition, a new shield-cup test method was proposed to address these problems. A fire detector evaluator (FDE) developed in a previous study was applied to ensure measurement accuracy and reproducibility. During the plunge tests, a significant measurement error was observed in the activation time of the smoke detector because of the rapid flow change when the detector was input. However, during the shield-cup tests, slight changes occurred in the flow inside the FDE when the detector as exposed to smoke. In conclusion, the proposed shield-cup test method is expected to be useful for evaluating the response characteristics of fire detectors more accurately in simulated fire environments.

• Fire Science and Engineering
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• v.34 no.3
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• pp.18-27
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• 2020

An actual fire test was performed on single combustibles placed in a local cinema complex, and quantitative differences in the maximum heat release rate (HRR) and fire growth rate were investigated based on the design fire curve methods (i.e., the general and 2-stage methods). In terms of combustible use and fire load, a total of 12 combustibles were selected, classified into cinema lounge and movie theater. It was found that the maximum HRR and fire growth rate determined using the two-stage method were quantitatively different from those of the general method. The application of the two-stage method, which can be used to determine the fire growth rate of the initial fire stage more precisely, could be useful in accurately predicting the activation time of fire detectors and fire-extinguishing facilities, as well as the available safe egress time (ASET) and required safe egress time (RSET).

• Journal of the Korea institute for structural maintenance and inspection
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• v.26 no.3
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• pp.64-71
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• 2022

In this study, fire and egress simulation was conducted for the egress safety evaluation of the nursing hospitals. A fire simulation was performed with or without the smoke exhaust system using the FDS, and the available safe egress time (ASET) of the nursing hospitals was calculated. In addition, an egress simulation considering the characteristics of occupants and egress delay time was performed using Pathfinder, and the required safe egress time (RSET) was calculated. By comparing the ASET and RSET, the egress safety of the nursing hospital with or without a smoke exhaust system was evaluated according to the number of egress guides and the egress delay time. The simulation results show that the number of casualties increased as the egress delay time increased, and the required safe egress time decreased as the number of egress guides increased. In addition, it was found that if a smoke exhaust system with the capacity specified in the KFPA is secured, the available safe egress time can be greatly increased and the number of casualties can be greatly reduced.

• Journal of Applied Biological Chemistry
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• v.65 no.4
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• pp.383-386
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• 2022

A gene encoding thermostable cellulase B (TmCelB) was isolated from Thermotoga maritima. The open reading frame (ORF) of TmCelB gene was 825bp long which predicted to encode 274 amino acid residues with a molecular weight of 31,732 Da. The 17 amino acid residues from N-terminal of the TmCelB was known as signal peptides. To analyze the enzymatic activity and biochemical properties, the ORF of TmCelB gene excluding a putative signal sequence encoding 17 amino acids were introduced into the E. coli expression vector, pRSET-B, and overexpressed in E. coli BL21. The optimum temperature of recombinant TmCelB was around 95 ℃, and the optimum pH of recombinant TmCelB was around pH 4.5. The recombinant TmCelB was stable at temperature below 100 ℃.

• Biotechnology and Bioprocess Engineering:BBE
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• v.10 no.4
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• pp.304-308
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• 2005

The response of IPTG induction was investigated through the monitoring of the alkali consumption rate and buffer capacity during the cultivation of recombinant E. coli BL21 (DE3) harboring the plasmid pRSET-LacZ under the control of lac promoter. The rate of alkali consumption increased along with cell growth, but declined suddenly after approximately 0.2 h of IPTG induction. The buffer capacity also declined after 0.9 h of IPTG induction. The profile of buffer capacity seems to correlate with the level of acetate production. The IPTG response was monitored only when introduced into the mid-exponential phase of bacterial cell growth. The minimum concentration of IPTG for induction, which was found out to be 0.1 mM, can also be monitored on-line and in-situ. Therefore, the on-line monitoring of alkali consumption rate and buffer capacity can be an indicator of the metabolic shift initiated by IPTG supplement, as well as for the physiological state of cell growth.