• Title/Summary/Keyword: RS level

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Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter?

  • Ferreri, Miro;Gao, Jian;Ren, Gaixian;Chen, Liben;Su, Jingliang;Han, Bo
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.2
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    • pp.173-180
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    • 2011
  • Osteocalcin (OC), a marker of bone turnover, displays patterns in relation to physiological and genetic factors. Here, we present an association study in a population of Chinese Holstein cattle (n = 24) with OC serum concentration as a phenotypic trait. We hypothesised that OC status is associated with phylogeny, vitamin D serum level and single nucleotide polymorphisms (SNPs). Mitochondrial DNA (mtDNA) was used as an unlinked marker to examine phylogeny and linkage to measured phenotypic traits of vitamin D and OC status. Following an association study with OC serum variability as the trait, genotyping of SNPs (n = 27) in OC-related genes was performed. Candidate SNPs were chosen in genes with an emphasis on the vitamin D and vitamin K pathways. Multivariant factor analysis revealed a correlation between vitamin D serum concentration and a SNP in the gene GC (rs43338565), which encodes a vitamin D-binding protein, as well as between a SNP in NFATc1 (rs42038422) and OC concentration. However, univariate analysis revealed that population structure, vitamin D serum levels and SNPs were not significant determinants of OC status in the studied group.

Development of Embedded Vessel Monitoring System Using NMEA2000 (NMEA2000을 이용한 임베디드 선박 모니터링 시스템의 개발)

  • Lee, Chang-Ui;Kim, Dal-Yong;Yu, Yung-Ho;Shin, Ok-Keun
    • Journal of Advanced Marine Engineering and Technology
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    • v.33 no.5
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    • pp.746-755
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    • 2009
  • Recently, NMEA2000 became the de facto standard of the communication protocol for the instrument level network in vessels, and it is anticipated that most of the vessels will be equipped with the sensors and actuators based on this protocol. The conventional serial communication specifications such as RS-232, RS-485, or even NMEA0183 are single-talker/multiple-listener and interconnection among multiple sensors and/or actuators usually results in a bundle of wires. On the other hand, NMEA2000 is a true bus type(multiple-talker/multiple-listener) protocol and theoretically up to 256 instruments can be hot-plugged. With the advent of the protocol, it is necessary to consider a new type of monitoring system for the instruments in vessels: In most traditional serial communication environments, a dedicated monitor or user interface was necessary for each sensor or actuator as it was simply not easy to collect data in a single place. With NMEA2000, the collection of various types of information is simple. An embedded system can be employed to combine, to arrange and to display informations from various types of devices. Assuming that the actuators and sensors on a vessel are interconnected by NMEA2000 standard, we devise a tentative interactive vessel monitoring system in which the following 4 kinds of informations are collected and delivered: the navigation, engine, weather and tanks informations.

Regulation of the sufABCDSE Operon by Fur

  • Lee, Joon-Hee;Yeo, Won-Sik;Roe, Jung-Hye
    • Journal of Microbiology
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    • v.41 no.2
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    • pp.109-114
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    • 2003
  • A promoter that is inducible by paraquat and menadione, the superoxide generators, independently of soxRS has been found in front of the sufABCDSE operon in Escherichia coli. Based on the observation that SufA is a holomog of IscA that functions in the assembly of iron sulfur cluster and the sufA promoter (sufAp) contains a putative Fur-binding consensus, we investigated whether this gene is regulated by Fur, a ferric uptake regulator, When examined in several sufAp-lacZ chromosomal fusion strains, sufAp was induced by EDTA, an iron chelator and a well-known Fur-inducer, The basal level of sufA expression increased dramatically in fur mutant, suggesting repression of sufAp by Fur. The derepression in fur mutant and EDTA-induction of sufA expression required nucleotides up to -61, where a putative Fur box is located. Purified Fur protein bound to the DNA fragment containing the putative Fur box between -35 and -10 promoter elements. The regulation by Fur and menadione induction of sufAp acted independently. The rpoS mutation increased sufA induction by menadione, suggesting that the stationary sigma factor RpoS acts negatively on sufA induction.

Expression of resveratrol synthase gene and accumulation of resveratrol in transgenic potatoes (Solanum tuberosum L.)

  • Yi, Jung Yoon;Seo, Hyo Won;Yun, Song Joong;Ok, HyunChoong;Park, YoungEun;Cho, Ji Hong;Cho, HyunMook
    • Korean Journal of Breeding Science
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    • v.41 no.4
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    • pp.385-390
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    • 2009
  • A resveratrol synthase (RS) gene was isolated from peanut (Arachis hypogaea, L. cv. Jinpoong) plants. This gene was placed under the control of the cauliflower mosaic virus 35S promoter (CaMV35S) and introduced into two Korean varieties of potato (Solanum tuberosum L. cvs. Jasim and Jowon) plants by Agrobacterium-mediated gene transfer. Putative transformants were screened by PCR with primers designed from CaMV 35S promoter, NOS terminator and RS gene. Most of selected transgenic potato plants showed the amplification of expected fragments by PCR of genomic DNA with gene-specific primers, while they were absent in untransformed control plants. Expression of the resveratrol synthase gene was also examined by northern blot analysis. The transformants showed a band which was lacking in the control plant, confirming that the introduced gene is transcribed into mRNA in the transformants. The strength of the band, which reflected the level of mRNA expression, differed among the individual transformants. Among the transformants obtained, the highest trans-resveratrol content in the transgenic young leaves of purple-fleshed "Jashim" was $2.11{\mu}gg^{-1}$ fresh weight and that in the microtubers in vitro of purple fleshed "Jashim" was $8.31{\mu}gg^{-1}$ fresh weight. This amount of resveratrol may have a positive biological effect on human health.

Using High Brightness LED Light Source Controller for Machine Vision (고휘도 LED를 이용한 머신비전용 조명광원 제어기 개발)

  • Park, Yang-Jae
    • Journal of Digital Convergence
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    • v.12 no.4
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    • pp.311-318
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    • 2014
  • This paper is to introduce a lighting source controller using high brightness LED to create a clear and reliable condition for an accurate measurement and testing, which is a core technology in clinical image system and mechanical automation system. This controller is designed to supply a stable power in a constant-current system by installing a high brightness LED driver, and to improve the reproducibility of brightness by using 32-bit ARM processor core, dividing brightness quantity into 256 levels, making the remote control and the external interface possible, and preventing and digitizing the brightness inaccuracy caused by errors of resistance values. This controller enables the lighting range to be wide and possible in a low lighting level compared to analog, adds the RS-485 communication function, and makes it for the users to control the on-off function and the dimming level by receiving date from an external device.

INFLUENCE OF AGE AT FIRST CALVING ON THE MILK PRODUCTION OF NATIVE AND CROSSBRED DAIRY COWS

  • Khan, U.N.;Benyshek, L.L.;Ahmad, M.D.;Chaudhary, M.Z.;Athar, S.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.2 no.4
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    • pp.565-570
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    • 1989
  • The influence of age at first calving on the milk production of crossbred dairy cows produced under major dairy cattle crossbreeding projects in Pakistan was studied from the year 1974 to 1980. These animals were bred and raised at the Livestock Production Research Institute, Bahadarnagar (LPRI), Livestock Experiment Station, Karachi (LES) and the University of Agriculture, Faisalabad (UAF). Local Sahiwal (SWL) cattle were crossed with the European breeds, Holstein Friesian (HF) and Jersey (J) at LPRI and UAF. At LES, native Red Sindhi (RS) were crossed with the HF and J breeds. At LES and UAF, the crossbred progeny, thus, produced comprised of halfbreds only while at LPRI 3/4 HF, 3/4 J, 1/4 HF and 1/4 J groups were also available for this study. The average age at first calving was considerably higher among the native breeds. At LES, the RS calved for the first time at an age of approximately 56 months. Corresponding values for SWLs at LPRI and UAF were 44 and 64 months, respectively. The 1/2 HF and 1/2 J crossbreds calved first at the age of approximately 25, 26, 34, 36, 37 and 38 months at LPRI, LES and UAF respectively. For 3/4 HF, 3/4 K, 1/4 HF and 1/4 J groups of LPRI the average age at first calving was 29, 26, 34 and 33 months. Considerable differences in age at first calving between the crossbreds and native breeds were observed. Due to early maturity, the former attained the peak level of milk production in third lactation whereas the latter groups, because of late maturity, reached this level in their fourth lactation. This study suggested that early maturity in Zebu cattle (Box indicus) could be induced through crossbreeding with European cattle (Bos Taurus).

Construction and Characterization of Vector Expressing Low Level of Translation Factor eIF5B (단백질합성인자 eIF5B의 저 발현 효모벡터의 제조 및 특성)

  • 최상기;송진희;이준행;이병욱;성치남
    • Korean Journal of Microbiology
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    • v.40 no.1
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    • pp.7-11
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    • 2004
  • eIF5B is a translation initiation factor that delivers Met-$tRNA^{Met}$ to AUG start codon and subsequently joins the small and large ribosomes. In order to study the function of eIF5B encoded by FUN12, we constructed FUN12 which lacked 5' end of its sequence. We found that this construct lacking almost all of its promoter in pRS plasmid partially complemented slow growth phenotype of fun12 deletion strain. Interestingly, this construct expressed N-terminally truncated eIF5B and its expression level was about 5% of that of wild type eIF5B. Low amount of the eIF5B expressed additionally in fun12 deletion strain played a direct role as a limiting factor for its growth. This limiting factor eIF5B in those strains also modulates activities of overall translation in vitro.

Assessment of National and Regional Plans Using Integrated Management Index of Korea National Planning and Environmental Planning for Present Status Evaluation (국토계획과 환경계획 통합관리 지표의 적용을 통한 통합관리 현황 평가)

  • Heo, Han-Kyul;Lee, Dong-Kun;Sung, Hyun-Chan;Heo, Min-Ju;Park, Jin-Han
    • Journal of the Korean Society of Environmental Restoration Technology
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    • v.22 no.4
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    • pp.81-91
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    • 2019
  • Integrated management of Korea national and environmental planing for sustainable development is suggested, and basic research is needed. In this study, national and regional plans were assessed using 'integrated management index of Korea national planning and environmental planning' to grasp the current status of integrated management on Korea national planning and environmental planning. As a result of the assessment, it was found that both national and regional plans need to improve considering the natural ecology part and water resource and quality part. In addition, it was derived that the detailed contents of the indicator can not be reflected according to the characteristics in the higher-level plan. Therefore, it has been found necessary to include proclamatory contents so as to be able to establish a detailed plan that reflects environmental goals in the lower-level plan.

Application of Remote Sensing and Geographic Information System in Forest Sector (원격탐사와 지리정보시스템의 산림분야 활용)

  • Lee, Woo-Kyun;Kim, Moonil;Song, Cholho;Lee, Sle-gee;Cha, Sungeun;Kim, GangSun
    • Journal of Cadastre & Land InformatiX
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    • v.46 no.2
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    • pp.27-42
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    • 2016
  • Forest accounts for almost 64 percents of total land cover in South Korea. For inventorying, monitoring, and managing such large area of forest, application of remote sensing and geographic information system (RS/GIS) technology is essential. On the basis of spectral characteristics of satellite imagery, forest cover and tree species can be classified, and forest cover map can be prepared. Using three dimensional data of LiDAR(Light Detection and Ranging), tree location and tree height can be measured, and biomass and carbon stocks can be also estimated. In addition, many indices can be extracted using reflection characteristics of land cover. For example, the level of vegetation vitality and forest degradation can be analyzed with VI (vegetation Index) and TGSI (Top Grain Soil Index), respectively. Also, pine wilt disease and o ak w ilt d isease c an b e e arly detected and controled through understanding of change in vegetation indices. RS and GIS take an important role in assessing carbon storage in climate change related projects such as A/R CDM, REDD+ as well. In the field of climate change adaptation, impact and vulnerability can be spatio-temporally assessed for national and local level with the help of spatio-temporal data of GIS. Forest growth, tree mortality, land slide, forest fire can be spatio-temporally estimated using the models in which spatio-temporal data of GIS are added as influence variables.

Predictors of Persistence and Adherence with Secondary Preventive Medication in Stroke Patients (지역사회 뇌졸중 환자들의 이차 예방을 위한 치료 지속률과 약물 순응도 관련 요인)

  • Kim, Young Taek;Park, Ki Soo;Bae, Sang-Geun
    • Journal of agricultural medicine and community health
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    • v.40 no.1
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    • pp.9-20
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    • 2015
  • Objectives: This study aimed to explore the persistence and adherence to secondary preventive medication of stroke patients after discharge and to assess the reasons for persistence and nonadherence. Methods: Four hundred twenty-nine patients with stroke were surveyed to determine their behaviors from discharge. Reasons for stopping medications were ascertained. Persistence was defined as continuation of all secondary preventive medications prescribed at hospital discharge, and adherence as continuation of prescribed medications according to health care provider instructions. Results: Of the 429 patients, 86.5% were treatment persistent and 41.2%(non-intentional nonadherence=39.4%, intentional nonadherence=19.4%) were adherent. Independent predictors of persistence included having experience about health education. Independent predictors of non-intentional nonadherence were modified Rankin Scale(mRS) (Exp(B)=2.858, p=0.001) and health education experience (Exp(B)=0.472, p=0.032), and independent predictors of intentional nonadherence were mRS (Exp(B)=2.533, p=0.006), depressive symptoms (Exp(B)=1.113, p=0.016), beliefs about medications questionnaire(necessity, Exp(B)=0.879, p=0.011, concern, Exp(B)=1.098, p=0.019). Conclusions: Although up to one-ninth of stroke patients continued secondary prevention medications, nonadherence is common. Several potentially modifiable patient, provider, and system-level factors associated with persistence and adherence may be targets for future interventions. Specially, interventions to improve adherence should target patients' beliefs about their medication.