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http://dx.doi.org/10.5713/ajas.2011.10166

Variability of Osteocalcin Status in Chinese Holstein Cattle: Do Phylogeny, Vitamin D or Gene Polymorphisms Matter?  

Ferreri, Miro (College of Veterinary Medicine, China Agricultural University)
Gao, Jian (College of Veterinary Medicine, China Agricultural University)
Ren, Gaixian (College of Veterinary Medicine, China Agricultural University)
Chen, Liben (College of Veterinary Medicine, China Agricultural University)
Su, Jingliang (College of Veterinary Medicine, China Agricultural University)
Han, Bo (College of Veterinary Medicine, China Agricultural University)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.24, no.2, 2011 , pp. 173-180 More about this Journal
Abstract
Osteocalcin (OC), a marker of bone turnover, displays patterns in relation to physiological and genetic factors. Here, we present an association study in a population of Chinese Holstein cattle (n = 24) with OC serum concentration as a phenotypic trait. We hypothesised that OC status is associated with phylogeny, vitamin D serum level and single nucleotide polymorphisms (SNPs). Mitochondrial DNA (mtDNA) was used as an unlinked marker to examine phylogeny and linkage to measured phenotypic traits of vitamin D and OC status. Following an association study with OC serum variability as the trait, genotyping of SNPs (n = 27) in OC-related genes was performed. Candidate SNPs were chosen in genes with an emphasis on the vitamin D and vitamin K pathways. Multivariant factor analysis revealed a correlation between vitamin D serum concentration and a SNP in the gene GC (rs43338565), which encodes a vitamin D-binding protein, as well as between a SNP in NFATc1 (rs42038422) and OC concentration. However, univariate analysis revealed that population structure, vitamin D serum levels and SNPs were not significant determinants of OC status in the studied group.
Keywords
Osteocalcin; Vitamin D; Population Structure; Single Nucleotide Polymorphisms; D-loop; Bos taurus;
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