• BMB Reports
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• 제44권8호
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• pp.529-534
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• 2011

Ribosomal protein S3 (rpS3) is a multifunctional protein involved in translation, DNA repair, and apoptosis. The relationship between rpS3 and cyclin-dependent kinases (Cdks) involved in cell cycle regulation is not yet known. Here, we show that rpS3 is phosphorylated by Cdk1 in G2/M phase. Co-immunoprecipitation and GST pull-down assays revealed that Cdk1 interacted with rpS3. An in vitro kinase assay showed that Cdk1 phosphorylated rpS3 protein. Phosphorylation of rpS3 increased in nocodazole-arrested mitotic cells; however, treatment with Cdk1 inhibitor or Cdk1 siRNA significantly attenuated this phosphorylation event. The phosphorylation of a mutant form of rpS3, T221A, was significantly reduced compared with wild-type rpS3. Decreased phosphorylation and nuclear accumulation of T221A was much more pronounced in G2/M phase. These results suggest that the phosphorylation of rpS3 by Cdk1 occurs at Thr221 during G2/M phase and, moreover, that this event is important for nuclear accumulation of rpS3.

• Bulletin of the Korean Chemical Society
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• 제27권1호
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• pp.59-64
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• 2006

Unlike other viral polymerases, HCV RNA-dependent RNA polymerase (RdRp) has not been successfully inhibited by nucleoside analogues presumably due to its strong substrate specificity for RNA. Thus, in order to understand the RNA-specificity of HCV RdRp, the structural characteristics of the active site was investigated. The hereto unknown 2-OH binding pocket at the active site of RdRp provides invaluable implication for the development of novel anti-HCV nucleoside analogues.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2001년도 춘계학술대회 논문집
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• pp.16-19
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• 2001

Rapid Prototyping (RP) and Rapid Tooling (RT) were introduced to reduce time-to-market and cost by shortening not only the development phase but also the production phase of the manufacturing process. RP generally builds up a prototype layer by layer, rapidly generating a fully three-dimensional free form shape. RT enables the manufacture of production tools. The integration of RP and RT has the potential for rapid net shaping of thee-dimensional parts, which have geometrical complexity. In this study, net shaping techniques for making three-dimensional parts using RP and RT are described and a sample part are shown. A three-dimensional metal part is manufactured by a new RP process, Variable Lamination Manufacturing by using Expandable Polystyrene Foam (VLM-S), and its application to RT for making a clover punch. In addition, we discussed the technology fusion between metal forming md RP/RT.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1271-1277
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• 2008

The effects of feeding soybean oil (SBO) or rumen protected conjugated linoleic acid (RP-CLA) on CLA accumulation in milk, and performance of lactating dairy cows were studied. Twenty four Holstein Friesian crossbred lactating dairy cows, averaging $126{\pm}45days$ in milk, $15.6{\pm}2.43kg$ of milk and $452{\pm}51kg$ body weight were stratified randomly and assigned in a randomized complete block design (RCBD) to three treatments of 8 cows each. The treatments were control, 150 g of SBO and 150 g of RP-CLA supplementation. Performance parameters showed that DM intake, NELP intake and body weight change were similar across treatments, while CP intake was decreased by SBO and RP-CLA supplementation. Milk yield and milk composition were not significantly different among treatments, except for milk fat percentage and fat yield which were significantly decreased by 27% (p<0.05) and by 28% (p<0.01), respectively, by RP-CLA supplements compared with control treatment. Feeding RP-CLA reduced 3.5% FCM compared with the other treatments (p<0.003). Both SBO and RP-CLA supplementation reduced ${\geq}C18:0$ and CLA concentration in milk fat.

• 한국생산제조학회지
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• 제9권1호
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• pp.68-74
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• 2000

The STL file format is an approximation of 3-D model with triangular facets. STL is a standard input file format of Rapid Prototyping(RP) equipment In computer graphics a circle has been approximated with an inscribed polygon which causes an error between the real and approximated circles. In this study an intersecting polygon has been used to approximate the circle and applied to produce more accurate RP part. The newly proposed method shows it's excellence in part accuracy.

• Journal of Animal Science and Technology
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• 제48권5호
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• pp.663-670
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• 2006

국내에서 제조된 서방출형 유전자재조합 돼지 성장호르몬 (porcine somatotropin; pST)이 돼지의 성장과 등지방두께에 미치는 영향을 구명하기 위해 평균체중이 78kg인 랜드레이스 거세돈 48두를 공시하여 대조구, 4:1제형 rpST구 (6회 투여), 1:1제형 rpST구 (4회 투여), 4:1제형 rpST구 (4회 투여)에 각각 12두씩 배치하였다. 대조구는 pST를 투여하지 않았고, rpST 처리구는 1주일에 1회, 목 근육에 서방형 제재를 주입하였는데, 6회 투여구는 개시부터 2주까지는 100mg을, 3주부터 6주까지는 125mg을, 4회 투여구는 개시부터 2주까지는 100mg을, 3주부터 4주까지는 125mg을 투여하였다. 시험사료는 라이신 함량이 0.9%인 시판 육성돈사료를 무제한 급여한 결과 (주) LG 화학에서 제조한 서방출형 유전자재조합 pST는 증체량 및 사료요구율을 개선시켜 생산성 향상과 등지방두께 감소로 육질이 향상되었다.

• 한국산학기술학회논문지
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• 제8권4호
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• pp.775-780
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• 2007

쾌속조형기술(Rapid Prototyping Technology)은 설계단계에 있는 3차원 모델을 시제품(Prototype)으로 다른 중간과정 없이 빠르게 생성하는 새로운 기술을 말한다. CAD 모델 데이터는 3차원 형상 시스템이지만 모니터 화면이나 문서로 출력할 때는 2차원으로 밖에 표시되지 않는다. 반면에 쾌속조형기술은 설계된 3차원 CAD 데이터를 2차원 단면 데이터로 변환한 후 RP(Rapid Prototyping)공정을 적용하여 순차적으로 적층해 감으로써 CAD 데이터와 같은 3차원 입체 형상을 제작하게 된다. 그러나 RP 시스템은 매우 가격이 비싸서 칫솔이나 완구 등과 같은 생활용품을 제작하는 중소기업에서는 구입하기 어려운 상황이다. 본 논문에서는 오픈소스를 사용하여 RP 기술을 구현함으로써 저렴한 가격으로 구매할 수 있는 RP 시스템을 개발하기 위한 3차원 제어시스템을 제안하였다.

• 한국식생활문화학회지
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• 제25권6호
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• pp.788-794
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• 2010

Camellia sinensis L. (green tea) seed oils were prepared by roasting at $213^{\circ}C$ and pressing (RP), pressing (P), and nhexane extraction (H). The physico-chemical properties of the RP, P, and H samples, including fatty acid composition, color, and sensory characteristics were analyzed. RP, P and H samples were thermally oxidized at $180^{\circ}C$, and oxidative stability was determined by DPPH, CDA, and p-AV at 0, 20, 40, 60, and 80 min. Compared to the P and H samples, RP resulted in significantly higher thermal oxidative stability according to the DPPH, CDA, and p-AV results (p<0.05). The ratio of unsaturated fatty acids to saturated fatty acids among RP, P, and H samples were significantly different (p<0.05). The oleic acid and linoleic acid contents in green tea seed oils were 58 and 23%, respectively. Hunter's color value of lightness (L) for the RP, P, and H samples was not significant. Redness (a) of RP was $3.47{\pm}0.119$ and yellowness (b) of H was $60.10{\pm}2.483$, which were significantly different. Compared to RP samples, H and P samples had the highest color and off-odor values in the sensory evaluation. RP samples showed the highest taste value and were significant overall (p<0.05). The thermal stability of RP extraction was more stable than any other method. Camellia sinensis L. seed oil extracted by RP had better sensory characteristics than other edible oils, including soybean oil, grape seed oil, and extra virgin olive oil.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 2007년도 춘계학술대회 논문집
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• pp.187-190
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• 2007

The objective of this paper is to investigate into the development of injection mould with high cooling characteristics using a direct metal RP technology. In order to manufacture the injection mould with a high cooling rate, three-dimensional conformal cooling channels have been generated in the mould. DMT process, which is one of direct metal RP technologies, has been utilized to directly manufacture the metallic mould with three-dimensional conformal cooling channels. In order to examine the performance of the designed mould, injection molding tests have been carried out. The results of the experiments have been shown that a cooling time and the injection time of the proposed mould are reduced by the factor of five and two times in comparison with the injection mould with linear cooling channels.

• Environmental Analysis Health and Toxicology
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• 제16권4호
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• pp.189-196
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• 2001

Octanol/water partition coefficients of 52 chemicals were calculated using RP-HPLC estimation method and predicted by computer program, PCHEM. The result showed relationship between literature values and RP-HPLC observed values (relative coefficient r$^2$＝0.916), but the relationship of PCHEM values with literature values was lower than RP-HPLC value (relative coefficient r$^2$＝0.795). The average difference in partition coefficient between the RP-HPLC method and flask-shaking method was log Kow＝0.54, while the average difference between the values predicted form the computer program and flask- shaking method was log Kow = 0.36 Compared to octanol/water partition coefficients by 3 methods (Flask-shaking, RP-HPLC, computer prediction), the octanol/water partition coefficient values based on the flask-shaking method were very similar to the literature values, while the octanol/water partition coefficient values by RP-HPLC method without to consider the dead time, and computer prediction values did not significantly differ with the literature values.