• Korean Journal of Clinical Pharmacy
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• v.5 no.2
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• pp.71-74
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• 1995

A high performance liquid chromatographic method has been developed for the simultaneous analysis of non-steroidal anti-inflammatory drugs in plasma. The simultaneous determination of ibuprofen, fenoprofen and ketoprofen is performed by RP-HPLC with UV detection. The chromatographic system consisted of Spherisorb octyl column$(5{\mu}m)$ ; the mobile phase was $acetonitrile\;-\;0.5\%$ phosphoric acid(55 : 45, v/v) and the detection wavelength was 230nm. Tolmetin was employed as an internal standard. The method described is rapid and simple with sensitivity limits of $2.0{\mu}g/ml$ ibuprofen, $0.5{\mu}g/ml$ fenoprofen and $0.3{\mu}g/ml$ ketoprofen and is suitable for routine clinical and pharmacokinetic studies.

• Archives of Pharmacal Research
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• v.17 no.1
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• pp.48-50
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• 1994

Three p-coumaroylamino acids (p-CAAs) were isolated from the yeast-elicited Ephedra distachya cultures by consecutive purification using XAD_2, silicagel and RP-HPLC. Retention times on HPLC as well as their UV, IR, NMR and MS spectral data indicated that the yeast-induced p-CAAs wre p-coumaroyl--D-valine, p-coumaroyl-D-serine and p-coumarouyl-D-threonine, respectively. The structures of p-CAAs were confirmed by the comparison of their physico-chemical properties 3with those of synthetic ones. They were isolated and identified for the first time from natural products and supposed to be accumulated as phytoalexins of Ephedra.

• Korean Journal of Pharmacognosy
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• v.30 no.1
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• pp.48-53
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• 1999

Isolation and quantitative determination of costunolide from Saussurea lappa Clarke (Compositae) has been conducted by using HPLC method. Costunolide in an acetone extract from the crude drug was separated on a RP-18 column using a $MeOH-H_20$ mixture (65:35) as an eluent and the average content is about $1.32{\sim1.42%.$ The content of costunolide in dried extract was decreased by about 24% in seven days. However it showed a slight decrease in solution. It is highly recommended that quantitative determination of costunolide from Saussureae Radix should be conducted as early as possible after solvent extraction.

• Journal of environmental and Sanitary engineering
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• v.7 no.1
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• pp.79-86
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• 1992

Rapid determination of chlorpyrifos and fluvalinate in mixed insecticide was established by using high performance liquid chromatography. Result were as follows : 1. On Lichrosorb${\circledR}$ RP-8(10 ${\mu}m$) Column with on fluent of acetanitrile : water=75 . 25, sufficient separation of Chlorpyrifos and Fluorinate of base was obtained. 2.410td portion the diluted solution was injected in to the cdumn, and the effluent was monitored by on UV-Detector at 270 nm. 3. Relative Standard Deviation were 0.97% (chlorpyrifos) and 0.5% (fluorinate) under analysis conditions. 4. The UV-Detector response at 270 nm were linear (Y=421.8X-2090, $R^2=0.9997$ : Chlorpyrifos, Y= 1062.7X-4017.7, $R^2=0.9998$ : Fluorinate) over a range of 30~500 ${\mu}g/ml$.

• Korean Journal of Pharmacognosy
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• v.32 no.2 s.125
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• pp.153-156
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• 2001

The methods for isolation and quantitative determination of dioscin, a spirostanol triglycoside, from the roots of Smilax china have been developed. Isolation of dioscin was achieved by silica gel and RP-18 chromatography. The HPLC method used for quantitative determination of dioscin enabled the standardization of the crude drug. It suggests that the content of dioscin in Smilax china should be above 0.01%.

• Bulletin of the Korean Chemical Society
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• v.32 no.1
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• pp.77-82
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• 2011

A new high performance liquid chromatograpgy (HPLC) stationary phase that possesses an internal carbonyl functional group is synthesized by heterogeneous "graft from" method. This new stationary phase, poly (vinyl octadecanoate) grafted silica (Sil-2) is then characterized by different physico-chemical methods such as diffuse reflectance infrared fourier transform, suspension state $^1H$ NMR, solid state $^{13}C$ CP/MAS NMR, $^{29}Si$ CP/MAS NMR, elemental analysis and thermogravimetric analysis. Chromatographic properties of Sil-2 were evaluated under reversed phase condition by separating polycyclic aromatic hydrocarbons (PAHs) and comparing the chromatographic results with those on polymeric as well as monomeric octadecylated silica stationary phases.

• Natural Product Sciences
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• v.28 no.1
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• pp.18-26
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• 2022

In order to facilitate the quantification in autumnal Hamamelidaceae leaves, a HPLC method was used for the determination of two chlorophyll catabolites and their isomers: bilin-type (1) and bilinone-type (2) ones. The separation was done on a RP-C4 column with a gradient solvent system of 0.1% trifluoroacetic acid aqueous-methanol at the flow-rate of 0.2 mL/min and detected at 244 nm. The quantity of bilin-type (1) and bilinone-type (2) chlorophyll catabolite isomers from ten species of Hamamelidaceae autumnal leaves methanol extracts: Corylopsis pauciflora, Corylopsis spicata, Forthergilla major, Hamamelis intermedia, Hamamelis japonicum, Hamamelis japonicum var. flavopurpurscens, Hamamelis virginiana, Parrotiopsis jacquemontiana, Parrotia persica and X Sycoparrotia semidecidua were from 0.85 mg/g ~ 57.50 mg/g for bilin-type isomers (1) and 3.40 mg/g ~ 49.30 mg/g for bilinone-type isomers (2). The results obtained gave insight in quantitative bilintype (1) and bilinone-type (2) chlorophyll catabolite composition of the Hamamelidaceae plant species autumnal leaves.

• YAKHAK HOEJI
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• v.52 no.1
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• pp.7-11
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• 2008

A high performance liquid chromatographic (HPLC) method for the determination of glycyrrhizic acid was developed for the quality control of traditional herbal medicinal preparation Bu-Zhong-Yi-Qi-Tang (BZYQT), which is well-known herbal medicine used as tonic. RP-HPLC analysis was carried out using Capcell pak $C_{18}$ MG column $(5\;{\mu},\;150{\times}4.6\;mm)$ and a mobile phase consisting of acetonitrile and water containing 0.03% phosphoric acid (pH 2.46) at a flow rate of 1.0 ml/min. The optimum wavelength for the detection of the glycyrrhizic acid was found at 250 nm using diode-array UV/VIS detector. The glycyrrhizic acid in BZYQT shows good linearity $(r^2>0.999)$ in the range of $15\;{\mu}g/ml$ to 500 ${\mu}g/ml$. The limit of detection (LOD) was less than 5 ng and R.S.D for intra-day and inter-day reproducibility was less than 7%. The mean recovery of the glycyrrhizic acid was $97.3{\sim}113.0%$. These results suggest that the developed HPLC method is simple and efficient, and could be contributed for the quality control of commercial BZYQT products.

• Korean Journal of Medicinal Crop Science
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• v.24 no.1
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• pp.47-54
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• 2016

Background : A new extraction method-heated ultrasonic extraction was qualitatively and quantitatively analyzed for the extraction of major ginsenosides from ginseng extract; this new high-performance liquid chromatography (HPLC) method was compared with the official extraction method of Korean industrial standards and standard for health functional food. Methods and Results : Ginsenoside compounds were analyzed for 35 minutes by the new HPLC analysis method using a Halo$^{(R)}$ RP-Amide column. The new HPLC analysis method was validated by the measurement of intra-day and inter-day precision, accuracy, limit of detection (LOD), and limit of quantification (LOQ) of each ginsenoside. The correlation coefficients (r2) for the calibration curves of the ginsenoside compounds were over 0.9997 in terms of linearity. The heated ultrasonic extraction method using ultrasonication for 30 minutes at $50^{\circ}C$ yielded higher amount of ginsenosides than the extraction method of the Korean industrial standards owing to the enhancement of extraction efficiency. Conclusions : Compared to the other extraction methods, the heated ultrasonic extraction method yielded a higher amount of ginsenoside Rb1 than Rg1 index compounds for the quality evaluation of ginseng roots.

• Natural Product Sciences
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• v.17 no.4
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• pp.337-341
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• 2011

An HPLC (high-performance liquid chromatography)-PDA (photodiode array) detection method was established for the determination of naringin, hesperidin and neohesperidin in the fruit of Citrus paradisi and C. grandis. The flavonoids were separated in less than 20 min using an YMC RP 18 column with isocratic elution using acetonitrile and water (23 : 77, v/v) at a flow rate of 1 ml/min, and a PDA detector. The levels of naringin, hesperidin and neohesperidin were 1345.92, 950.62, and 2078.82 ${\mu}g/g$, respectively, in the peel, and 102.43, 59.13, and 86.68 ${\mu}g/g$, respectively, in the flesh of C. paradisi. In C. grandis, the levels of naringin, hesperidin and neohesperidin were 3530.56, 80.00, and 5.26 ${\mu}g/g$, respectively, in the peel, and 59.59, 7.43, and 38.41 ${\mu}g/g$, respectively, in the flesh. The total content was highest in the peel, reaching 0.44% and 0.36% in C. paradisi and C. grandis, respectively, while the flesh contained only 0.025% and 0.011%, respectively. Therefore, the peels of C. paradisi and C. grandis are necessary for the processing and utilization of flavonoids.