• Journal of Yeungnam Medical Science
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• v.1 no.1
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• pp.41-48
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• 1984

The soil bacterium Agrobacterium tumefaciens is a plant pathogen that cause3 crown gall tumors by infecting the wounded dicotyledonous plants and subsequent integration of bacterial DNA into plant nuclear DNA. Virulent A. tumefaciens strains harbor a large Ti (tumor-inducing) plasmid that carries genes essential for tumorigenesis. In the present study, 13 strains (Malus pumila Mill; $A_{1-3}$, Populus monilifera; $W_{1-6}$, Populus tomentiglandlosa; $P_{1-3}$ and Rosa species; $R_1$) of Agrobacterium isolated in korean crown gall tumors and plasmids were observed in 6 strains ($W_2$, $W_3$, $W_6$, $P_1$, $P_3$ and $A_2$). The test for crown gall tumor formation was resulted only in ATCC15955 and $KW_2$ strains inoculated into the stem of sun flower and the development was observed for 4 and 6 weeks after inoculation. Above two Ti plasmids (pTi) were purified by cesium chloride-ethidium bromide density gradient centrifugation and digested with restriction enzyme and fragments of pTiATCC 15955 and $pTiKW_2$ observed by EcoR I ; 25&27, Hind III; 23&21, BamH I ; each 20 and Hpa I ; 12&27, and sizes of pTiATCC15955 and and $pTiKW_2$ calculated as 200 and 87 kbases. Octopine was isolated from tumor tissue ($W_{1-6}$ and $P_{1-3}$) and these strains confirmed as octopine type.

• Korean journal of applied entomology
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• v.32 no.3
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• pp.338-347
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• 1993

The study was to investigate the bionomics, host plants, and oviposition preference of Hishimonus sellatus Uhler. It has 5 generations under natural conditions and the peak of the 3rd generation was observed about mid and late August. Its average developmental durations were 80 days in spring, 69 days in summer, and 77.8 days in autumn. The lengths of each stage were 0.8mm in eggs, 0.9mm in 1st instar, 1.4mm in 2nd instar, 2.1mm in 3rd instar, 2.5mm in 4th instar, 3.2mm in 5th instar, 4.1mm in female, and 3.8mm in male. Hishimonus sellatus overwintered as egg in Morus alba, Humulus japonicus, and Zizyphus jujuba begining mid October, and attacked the shoot of M. alba and H. japonicus about mid and late May, migrated to the Zizyphus jujuba from late June to early July. Female oviposites 32~62 eggs into epidermis of shoot, vagina and vein during their life. The preferred host plants of H. sellatus were Humulus japonicus, Morus alba, Zizyphus jujuba, and ligustrum obtusifolium. Highly preferable oviposition site was H. japonicus, M. albal, Z. jujuba, and L. obtusifolium, etc. On audlt longevity, the host plants as H. japonicus, M. alba, and Z. jujuba were 43$\pm$2 days and A. brevipedenculata, C. mimosoides, L. obtusifolium, V. rosa, A. sinicus and, A. graveolens were more than 25 days, and other host plants were less than 20 days.

• Natural Product Sciences
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• v.13 no.1
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• pp.33-39
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• 2007

Eleven polyhydroxyursane triterpenoids (PHUTs) were tested to determine their cytoprotective, immunosuppressive and anti-inflammatory effects. To compare the bioactivities of $19{\alpha}$-hydroxyursane-type triterpenoids {23-hydroxytormentic acid (6), its methyl ester (7), tormentic acid (8), niga-ichigoside $F_1$ (9),euscaphic acid (10) and kaji-ichigoside $F_1$ (11)} of the Rosaceae crude drugs (Rubi Fructus and Rosa rugosae Radix) with PHUTs possessing no $19{\alpha}-hydroxyl$ of Centella asiatica (Umbelliferae), the four PHUTs, asiaticoside (1), madecassoside (2), asiatic acid (3), and madecassic acid (4) were isolated from C. asiatica and 23-hydroxyursolic acid (5) from Cussonia bancoensis. Cytoprotective effects were assessed by measuring cell viabilities against cisplatin-induced cytotoxocity in $LLC-PK_1$, cells (proximal tubule, pig kidney) to determine whether these agents have protective effects against nephrotoxicity caused by cisplatin. The inhibitory effect of 11 PHUTS on nitric oxide (NO) and prostaglandin $E_2\;(PGE_2)$ were evaluated by measuring nitrite accumulation in lipopolysaccharide (LPS)-induced macrophage RAW 264.7 cells, and their anti-inflammatory effects were tested in 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema model. Six MHUTs (compounds 1, 2, 4, 6, 10, and 11) exhibited higher cell viabilities during cisplatin-induced cytotoxicity testing even at a concentration of $200\;{\mu}g/ml$ than cisplatin only-treated group, suggesting that ese compounds have the potentcytoprotective efffcts. Compounds 1 and 3 of the C. asiatica and niga-ichigoside $F_1$ exhibited no inhibitory effect on NO and/or $PGE_2$ production whereas other PHUTs produced mild to significant NO and/or $PGE_2$ production.The four compounds (2, 5, 9, and 10) potently inhibited mouse ear edema induced by TPA whereas two compounds (1 and 3) had no activity in this test. These results suggest that many PHUTs are potentchemopreventives. Structure-activity relationship (SAR) was also discussed in each assay with regard to the significant role of OHs at the position of 2, 3, 6, 19, and 23 and to the glycoside linkage at the 28-carboxyl.

• Journal of Wetlands Research
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• v.19 no.2
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• pp.193-201
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• 2017

This study was carried out to obtain basic ecological information required for wetland restoration in Korea where wetland is very deficient. To arrive at the objective, we collected the basic ecological information for wetland restoration in four Doombeongs located on Goesan of Chungcheongbuk-do (province), central Korea where maintains relatively integrate feature of Doombeong. Synthesized horizontal distribution of vegetation based on vegetation established naturally except that established by artificial interference, Potamogeton distinctus community, Spirodela polyrhiza community, Sagittaria aginashi community, Trapa japonica community, Scirpus triangulatus community, and Sparganium japonicum community, Persicaria thunbergii community, Juncus effusus var. decipiens community, Ludwigia prostrata community, Humulus japonicus community, Persicaria nodosa community, Miscanthus sacchariflorus community, Phragmites communis - P. japonica community, and Scirpus radicans community, S. gracilistyla community, Spiraea prunifolia for. simpliciflora community, and Rosa multiflora community, and Salix koreensis community and Acer tataricum subsp. ginnala community tended to be established in aquatic zone, herbaceous plant dominated vegetation zone, shrub dominated vegetation zone, and tree and sub-tree dominated vegetation zone, respectively. As the result of DCA ordination based on vegetation data collected from several Doombeongs and their surrounding areas, plant communities tended to be classified into aquatic, wetland, and riparian plant dominated stands. Spatial niche and species composition of major plant communities composing those stands were suggested as the reference information for creating ecological pond as a type of wetland. Further, the importance of wetland and the necessity of wetland restoration was discussed based on functions that the wetland displays.

• Journal of the Korean Institute of Landscape Architecture
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• v.21 no.1
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• pp.31-50
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• 1993

This study was conducted to investigate the plants status of the woody landscape plants species of 38 elementary, middle, and high school in Iri city, and to obtain some fundamental information for development and use of native species of trees. The results are summarized as follows: 1. Landscape plants species of 38 elementary, middle and high school in Iri city was 55 families, 164 species, and 85, 045 trees and the average of landscape plants species of 17 elementary schools was 50.5 families, 3, 010 trees, on the other hand, middle and high school was 42.4 families, 1, 609 trees. 2. Plants species by type was Deciduous Broadleaved Tree 32.9%, Deciduous Broadleaved Shrub 25.6%, Evergreen Coniferous Tree 14.6%, Evergreen Broadleaved Shrub 9.7%, Vine 4.8%. Evergreen Trees on the other hand, according to the utilization rate 'others' was 32.2%. 3. The rate of plants species of the Evergreen Trees to the Deciduous (except Vine and 'others') was 34:66. on the other hand, rate of quantity was 50.5:49.5 and the rate of the trees to the shrubs(except Vine and 'others') was 58:42. to the plants species and to the quantity of the number of trees was 25.9:74.1. 4. Plants species of fruit plants of 38 elementary, middle and high school in Iri city was 31 species to the 164 species. The rate of frequency was Poncirus trifoliata 27.8%, Lycium chinense 25.1%, Ginkgo biloba 16.7%, to the quantity of the number of trees, fruit plants was 2, 605 trees to the 85, 045 trees and the frequency was 3%. 5. School trees and flowers of 38 elementary, middle and high school in Iri city were appointed 17 elementary school, on the other hand, middle and high schools were only 4 middle and high schools. Plants species of the best of frequency school tree was Zelkova serrata and school flower was Rosa spp.. 6. The rate of the native species to the introduced species was 73.8:26.2. on the other hand, the rate of quantity by the number of trees was 84.7:15.3 to the type, the rate of frequency of plants of native species Evergreen trees was Thuja orientalis, deciduous was Ginkgo biloba, Vine was Wisteria floribunda and 'others' was Sasa borealis. on the other hand, to the introduced species, Evergreen Trees was Juniperus chinensis. Deciduous Trees was Metasequoia glyptostroboides, and Vine was Vitis labrusca and 'others' was Phyllostachys pubescens. 7. As the fundament of the results of above the optimum selecting of woody landscape plants according to native species of trees was 29 families, 58 species. on the basis of 30 points, 28 points was 8 species Ginkgo biloba, Ulmus davidiana var. japonica, Parthenocissus tricuspidata, Elaeagnus umbellata, Fraxinus rhynchophylla, Weigela subsessilis, Koelreuteria paniculata, Lonicera japonica, 26 points was 27 species Thuja orientalis, Zelk ova serrata, Euonymus japonica etc., and 24 points was 23 species Populus tomen tiglandulosa, Juglans sinensis, Sophora japonica etc..

• Current Research on Agriculture and Life Sciences
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• v.31 no.1
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• pp.56-60
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• 2013

This research was carried out to produce rainbow-colored roses from white cut rose, Rosa hybrida 'Denice', to meet customers' desire of having various colors of roses. The higher temperature of dyeing solution used, the faster coloring time surveyed. In high temperature, the flowering speed of cut flower accelerated and after treating more than 4 hours, the edges of flowers became too dark or dry. In the condition of concentration of dyeing solution, $11g{\cdot}L^{-1}$ dyed faster than $7.5g{\cdot}L^{-1}$, however, no differences between $15g{\cdot}L^{-1}$ and $11g{\cdot}L^{-1}$ have observed. It looked fast coloration at the stage of early blooming, but all three different petal opening stages need similar time to get even coloration from outer to inner petals. For the consideration of commercial value, flowers with 3-4 petals opened are better quality than flowers with less petals opened. It was possible to make rainbow-colored rose by dipping 'Denice' with 3 primary color combination, Hot Pink, True Blue, and Yellow dyes, at the best result conditions of $20^{\circ}C$ solution temperature, 3-4 petals opening stage, 11g/L concentration of dye solution and 3 hours dipping, respectively.

• The Korean Journal of Physiology and Pharmacology
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• v.24 no.4
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• pp.339-348
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• 2020

We aimed to characterize the participation of rapid non-genomic and delayed non-genomic/genomic or genomic mechanisms in vasoactive effects to triiodothyronine (T3), emphasizing functional analysis of the involvement of these mechanisms in the genesis of nitric oxide (NO) of endothelial or muscular origin. Influences of in vitro and in vivo T3 treatments on contractile and relaxant responsiveness of isolated rat aortas were studied. In vivo T3-treatment was 500 ㎍·kg^-1·d^-1, subcutaneous injection, for 1 (T3_1d) and 3 (T3_3d) days. In experiments with endothelium-intact aortic rings contracted with phenylephrine, increasing concentrations of T3 did not alter contractility. Likewise, in vitro T3 did not modify relaxant responses induced by acetylcholine or sodium nitroprusside (SNP) nor contractile responses elicited by phenylephrine or angiotensin II in endothelium-intact aortas. Concentration-response curves (CRCs) to acetylcholine and SNP in endothelium-intact aortic rings from T3_1d and T3_3d rats were unmodified. T3_3d, but not T3_1d, treatment diminished CRCs to phenylephrine in endothelium-intact aortic rings. CRCs to phenylephrine remained significantly depressed in both endothelium-denuded and endothelium-intact, nitric oxide synthase inhibitor-treated, aortas of T3_3d rats. In endothelium-denuded aortas of T3_3d rats, CRCs to angiotensin II, and high K⁺ contractures, were decreased. Thus, in vitro T3 neither modified phenylephrine-induced active tonus nor CRCs to relaxant and contractile agonists in endothelium-intact aortas, discarding rapid non-genomic actions of this hormone in smooth muscle and endothelial cells. Otherwise, T3_3d-treatment inhibited aortic smooth muscle capacity to contract, but not to relax, in an endothelium- and NO-independent manner. This effect may be mediated by delayed non-genomic/genomic or genomic mechanisms.

• Research in Plant Disease
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• v.14 no.3
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• pp.159-164
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• 2008

Crown gall of rose (Rosa hybrida) was observed in greenhouse during 2003-2007. The average disease incidence was up to 38.1 % and was more severe in hydroponic culture as compared to soil culture. The typical gall symptom occurred mainly on the root, crown, or both, and resulted on poor rooting, growth retardation and yield loss. The reduction rate of rooting influenced by crown gall was 57.5% as compared to healthy plants on nursery stock. The location of gall formation in the plant influenced growth vigor resulting in symptoms such as poor shooting. Healthy plants produced 19.1 flowers/$m^2$, while diseased plants produced 9.5 flowers/$m^2$ during the same cultivation period. Moreover, the number of days to flowering was longer for the diseased plants than for healthy plants - 51.2 days and 39.8 days for first harvest, and 60.6 days and 52.1 days for the second harvest, respectively. Conclusively, infection on the basal stem caused serious loss of the number of shoot formation; yield loss of cut flower was 38.7% due to crown gall infection and delay of harvesting time about 8-10 days.

• Korean Journal of Applied Biomechanics
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• v.19 no.1
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• pp.167-177
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• 2009

The purpose of this study was to investigate effect of golf shoe design on kinematic variables during golf swing. Five professional male golfers with shoe size 270mm were recruited for the study. Swing motion was collected using 8 high speed camera motion analysis at a sampling of 180Hz. Kinematic variables were calculated by EVaRT 4.2 software. Driver swing was divided into four events; El(adress), E2(top), E3(impact) and E4(finish). Time, peak velocity, velocity of center of mass, velocity of the foot and ankle angle during Phase 1(El-E2), Phase 2(E2-E3), and Phase 3(E3-E4) were analyzed in order to investigate the relationship between golf shoe design and swing performance. The findings indicated that type C golf shoes would be beneficial for stability and control of movement during address and swing performance. Furthermore, faster speed of golf shoes, center of mass, and both feet were observed with Type C golf shoes. It is expected that golfers with Type C golf Shoes provide greater force as they control the center of mass faster and increase rotational force during impact compared to other golf shoes.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.5
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• pp.358-364
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• 2001

To obtain information for the proper fertilization management of cut-flower roses, the amount of macro and micro elements absorbed by cut-flower roses from soil for a year was investigated. Three cut-flower rose cultivars which are called 'Grandgara', 'Nobless', and 'Centina' were transplanted to a sandy loam soil, N-P-K standard fertilization was applied to the soil, and drip irrigation was done at the level of 10 kPa soil moisture tension. There was not significantly different in the harvest amount of cut-flower rose between 'Grandgara' and 'Nobless', but the harvest yield of 'Centina' was about 63% level when compared to that of 'Grandgara'. Considering seasonal changes in the content of nutrients in plant, parts, the uptake of untrients was higher in winter season than that in spring and summer seasons. Except for 'Centina', the nutrient amount removed from plant parts of 'Grandgara' and 'Nobless' increased with the sequence of floral part < stem < leaf, indicating that it is more dependent on biomass yield than on the content of nutrients in each plant part. The ratio of N/K amount absorbed by 'Nobless' and 'Centina' was 1.13 and 1.28. respectively, lower than 1.68 of 'Grandgara', showing that the requirement for K is greater in 'Nobless' and 'Centina' than in 'Grandgara'. The use efficiency of nutrients by cut-flower roses ranged from 39 to 64% in nitrogen, 5 to 9% in phosphorus, and 37 to 67% in potassium. It suggests that the requirement for P in cut-flower roses is very low.