• 한국식품과학회지
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• 제36권4호
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• pp.624-630
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• 2004

발아에 의한 거대배아미와 유색미의 기능성 변화를 항산화 활성에 중심을 두고 조사하였다. 전반적으로 환원력은 일반현미보다 거대배아미와 유색미가 높았고, 발아처리는 환원력을 증가시키는 경향이 있었다. 지질과산화에 대한 억제효과를 조사한 결과, in vitro의 linoleic acid peroxidation system 및 ex vivo의 토끼 적혈구 막지질 과산화 system 모두에서 억제효과도 유색미가 가장 높았고, 그 다음이 거대배아미, 일반현미의 순서로 나타났다. Superoxide radical에 대한 소거활성은 유색미>거대배아미>일반현미의 순서로 낮아졌으며, 무발아 조건과 비교할 때 발아처리는 라디칼에 대한 소거활성을 증진시켰다. Hydroxyl radical 소거활성도 유색미가 가장 높았으며, 그 다음이 거대배아미, 일반현미의 순서였다. 발아에 의하여 일반현미의 hydroxyl radical 소거활성을 급격히 증가되었지만, 발아미간에 소거활성의 순위는 변하지 않았다. In vitro에서 조사된 ROS 소거활성과 같은 경향이 HL-60 세포에서 TPA로 자극으로 발생된 ROS에 대한 소거활성에서도 나타났다. 실험 결과, 유색미와 거대배아미에서는 발아와 연관되어 세포독성의 증가가 발견되지 않는 차별적인 ROS 소거활성의 증가 현상이 발견되었다.

• 대한화장품학회지
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• 제33권2호
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• pp.61-67
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• 2007

본 연구에서는 쇠뜨기 추출물의 항산화능을 조사하였다. 추출물의 free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) 소거활성($FSC_{50}$)은 50% 에탄올 추출물($182.04{\mu}g/mL$) < ethylacetate 분획(54.50) < 당을 제거시킨 플라보노이드 aglycone 분획(14.13) 순으로 증가하였다. Luminol-의존성 화학발광법을 이용한 $Fe^{3+}-EDTA/H_2O_2$ 계에서 생성된 활성산소종(reactive oxygen species, ROS)에 대한 총항산화능은 당을 제거시킨 플라보노이드 aglycone 분획($OSC_{50}$, $3.54{\mu}g/mL$) < 50 % 에탄올 추출물(0.80) < ethylacetate 분획(0.006) 순으로 ethylacetate 분획에서 가장 큰 활성을 나타내었다. 쇠뜨기 추출물에 대하여 rose-bengal로 증감된 사람 적혈구의 광용혈에 대한 보호효과를 조사하였다. 50% 에탄올 추출물의 경우 농도 의존적($1{\sim}100{\mu}g/mL$)으로 광용혈을 억제하였다. 특히 ethylacetate 분획에서 당을 제거시킨 aglycone 추출물은 $10{\mu}g/mL$ 농도에서 $\tau_{50}$이 161.10min으로 매우 큰 세포보호 활성을 나타내었다. 이상의 결과들은 쇠뜨기 추출물이 $^1O_2$ 혹은 다른 ROS를 소광시키거나 소거함으로써 그리고 ROS에 대항하여 세포막을 보호함으로써 생체계, 특히 태양 자외선에 노출된 피부에서 항산화제로서 작용할 수 있음을 가리킨다.

• 동의신경정신과학회지
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• 제20권1호
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• pp.21-42
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• 2009

Objectives : This Study was performed to assess the antioxidant and neuroprotective effect of Chunghyul-dan(Qingxuedan) in PC12 cells and primary rat mesencephalic dopaminergic neurons. Methods : The anioxidant effect was investigated using the DPPH radical and ABTS cation scavenging assays and total polyphenol amout of Chunghyul-dan(Qingxuedan). The neuroprotective effect of Chunghyul-dan(Qingxuedan) in PC12 cells was evaluated using MTT assay. The scavenging activity of Chunghyul-dan(Qingxuedan) on ROS production induced by 6-OHDA(6-hydroxydopamine) in PC12 cells was evaluated, as well as the attenuating effect on GSH reduction. Finally, we examined the neuroprotective effect of Chunghyul-dan(Qingxuedan) against 6-0HDA-induced toxicity in the primary culture of rat mesencephalic doperminergic neurons. Results : Chunghyul-dan(Qingxuedan) showed concentration-dependent scavenging activities in DPPH radical and ABTS cation scavenging assays and it was not cytotoxic to PC12 cells. In postand co-treatment, Chunghyul-dan(Qingxuedan) protected PC12 cells from the 6-OHDA induced toxicity at 50 and 100 ${\mu}$g/mL significantly. And Chunghyu!-dan(Qingxuedan) decreased the 6-OHDA induced ROS production at a dose dependent manner, while increaing the 6-OHDA induced GSH reduction at 50 and 100 ${\mu}$g/mL significantly. Finally, Chunghyul-dan(Qingxuedan) showed signicant protection of rat mescencephalic dopaminergic neurons from 6-OHDA at 1 ${\mu}$g/mL. Conclusions : These results demonstrate that Chunghyul-dan(Qingxuedan) has the antioxidant and neuroprotective effect against 6-0HDA induced cytotoxicity through decreasing ROS production and increasing GSH reduction.

• 한국응용과학기술학회지
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• 제39권5호
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• pp.710-719
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• 2022

본 연구는 Passiflora caerulea 추출물의 항산화 활성 및 멜라닌 생합성 억제 효과 측정을 통해 화장품 소재로서의 활용 가능성을 확인하고자 하였다. Passiflora caerulea 추출물은 70% 에탄올로 추출하였고, TEAC assay를 이용한 ABTS radical 소거능을 측정하였다. 또한 Neutral red assay를 이용한 세포 생존율, DCF-DA를 통한 세포 내 ROS 생성 억제, 멜라닌 생합성 억제 효과를 측정하였다. 연구결과, Passiflora caerulea 추출물의 ABTS radical 소거능은 농도 의존적 소거활성이 확인되었으며, positive control(양성대조군)로 이용한 trolox와 0.1 mg/mL 농도에서는 유사한 radical 소거 활성을 확인하였다. CFDA를 통한 세포 내 활성산소종(ROS) 생성은 농도 의존적으로 억제되었고, B16F10 melanoma 세포에 대한 세포 독성은 나타나지 않았다. 또한 𝛼-MSH로 유도된 멜라닌 생합성 억제 효과를 확인하였다. 따라서 본 연구를 통해 Passiflora caerulea 추출물은 화장품 소재로서의 활용 가능성이 있는 것으로 판단된다.

• 한국미생물·생명공학회지
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• 제44권2호
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• pp.117-123
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• 2016

본 연구에서는 C. pubescens Burkill 에탄올 추출물(CPEE)의 항산화능과 항염증 생리활성을 DPPH 라디칼 소거능, ROS 생성 억제능, NO 소거 활성 등을 통해 분석하였다. 먼저 CPEE의 항산화능을 DPPH 라디칼 소거능으로 분석한 결과 강한 소거능을 보였으며, 좀 더 자세한 항산화능 작용기작을 알아보기 위해 ROS 생성 억제능으로 분석한 결과 농도 의존적으로 강한 ROS 소거능을 보였다. 뿐만 아니라 대표적인 항산화 효소인 HO-1 및 그 전사 인자인 Nrf2의 단백질 발현에 미치는 영향을 분석한 결과 CPEE에 의해 HO-1 및 Nrf2의 발현이 증가됨을 보였다. 한편 CPEE가 LPS에 의해 유도된 NO 생성에 미치는 영향을 분석한 결과 농도의 존적인 NO 생성 저해능을 보였으며 이는 NO 생성 단백질인 iNOS의 발현 저해에서 기인함을 확인하였다. 이러한 결과를 통해 CPEE의 높은 항산화능과 항염증 활성을 확인하였으며 향후 잠재적인 기능성 소재로서 유용하게 활용될 수 있을 것으로 판단된다.

• Preventive Nutrition and Food Science
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• 제9권2호
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• pp.150-155
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• 2004

The free radical scavenging activities and the protective effects of Rhus javanica extracts against oxidative damage induced by reactive oxygen species (ROS) were investigated. n-Hexane, ethyl acetate and water fractions were prepared from a methanol extract. DPPH radical, superoxide anion and hydroxyl radical scavenging activities were estimated. Intracellular ROS formation was quantified using fluorescent probes, 2', 7'-dichlorofluorescin diacetate (DCFH-DA) for hydroxyl radical and dihydroethidium (DHE) for superoxide anion. The oxidative DNA damage was investigated by the comet assay in HepG$_2$ cells exposed either to $H_2O$$_2$ or to menadione. The highest $IC_{50}$/ values for DPPH radical scavenging activity was found in the ethyl acetate fraction with a value of 5.38 $\mu\textrm{g}$/mL. Cells pretreated with $\geq$ 1 $\mu\textrm{g}$/mL of the ethyl acetate extract had significantly increased cell viability compared to control cells, which were not pretreated with the extract. Intracellular ROS formation and DNA damage in HepG$_2$ cells, which were pretreated with the various concentrations of Rhus javanica ethyl acetate extract and then incubated either with $H_2O$$_2$ or with menadione, reduced in a dose-dependent manner. These findings suggest that Rhus javanica might have biologically active components which have strong protective effects against ROS induced oxidative damages to the biomolecules, such as cell membranes and DNA.

• 약학회지
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• 제45권1호
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• pp.101-107
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• 2001

Green tea catechins (GTC) and its major component, (-)-epigallocatechin gallate (EGCG) were studied for their protective effects against reactive oxygen species (ROS)-induced oxidative stress. GTC and EGCG skewed the strong antioxidative effects on the lipid peroxidation of ethyl linolate with Fenton's reagent and free radical scavenging effect to DPPH radical generation. They also protected $H_2O$$_2$- or KO$_2$-induced cytotoxicity in CHL cells or mouse splenocytes. These results indicate that GTC and EGCG are capable of protecting the lipid peroxidation, flee radical generation and cytotoxicity induced by ROS. The mechanism of inhibition in ROS-induced cytotoxicity may be due to their antiofidative and free radical scavenging properties. Therefore, GTC and EGCG may be useful chemopreventive agents by protecting the free radical generation which are involved in cancer and aging.

• ALGAE
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• 제20권3호
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• pp.251-260
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• 2005

Antioxidative activities of Chlorophyta and Phaeophyta in Jeju Island were measured by superoxide anion ($O_2^{{\cdot}-}$), hydroxyl radical ($HO^{\cdot}$), hydrogen peroxide ($H_2O_2$) and DPPH free radical scavenging assays. Methanolic and aqueous extracts of the seaweeds were prepared at both temperatures, higher (70$^{\circ}C$) and room temperature (20$^{\circ}C$), and screened for the construction of an extract bank from seaweeds in Jeju Island. A variety of extracts showed positive effect against reactive oxygen species (ROS). Especially, Sargassum thunbergii methanolic extract at 70$^{\circ}C$ (70ME, 97.41%), S. fulvellum methanolic extract at 20$^{\circ}C$ (20ME, 84.66%), Codium fragile aqueous extract at 70$^{\circ}C$ (70AE, 96.61%) and S. thunbergii 20ME (97.44%) exhibited the highest scavenging activities against $O_2^{{\cdot}-}$, $HO^{\cdot}$, $H_2O_2$ and DPPH free radicals, respectively. Total phenolic contents also examined but did not show a positive correlation with ROS scavenging abilities (except for a few extracts). These results indicate that further investigation is needed to identify and purify the responsible antioxidative components.

• 약학회지
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• 제49권1호
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• pp.86-91
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• 2005

Flavonoids are class of polyphenolic compounds widely distributed in the plant kingdom, which display a variety of biological activities, including antiviral, antithrombotic, antiinflammatory, antihistaminic, antioxidant and free-radica 1 scavenging abilities. The antioxidant enzyme (AOE) system plays an important role in the defense against oxidative stress insults. To determine whether flavonoid, myricetin can exert antioxidative effects not only directly by modulating the AOE system but also scavenging free radical, we investigated the influence of the flavonoid myricetin on cell viability, different antioxidant enzyme activities, ROS level and the expression of different antioxidant emzyme in B16F10 murine melanoma cells. Myricetin in a concentration range from 6.25 to $50\;{\mu}M$ decreased superoxide dismutase (SOD) and glutathione peroxidase (GPx) enzyme activities, but catalase (CAT) activity was increased. In the myricetin-treated group, ROS levels were decreased dose-dependently. Antioxidant enzyme expression was measured by RT-PCR. Myricetin treatment of B16F10 cells increased catalase expression. Expression levels of copper zinc superoxide dismutase (CuZn SOD) were not affected by exposure of myricetin. Manganese superoxide dismutase (Mn SOD) and GPx expression levels decreased slightly after myricetin treatment. In conclusion, the antioxidant capacity of myricetin was due to CAT and free-radical scavenging.

• International Journal of Industrial Entomology and Biomaterials
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• 제43권2호
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• pp.59-66
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• 2021

Silkworm pupa has been used as an edible insect with the high quality of protein and unsaturated fatty acids. In this study, antioxidant activities of pupa according to variety, pupation day, sex, and extraction solvent were analyzed. The 30% ethanol extract showed highest radical scavenging activity compared with the DW, hexane, and 70-100% ethanol extracts. In the DPPH and ABTS radical scavenging assay according to the type of pupa, the antioxidant effect was increased in female with the early stage of pupation day. In cell-based assay, reactive oxygen species (ROS) level was decreased in pupa groups by -30 to -50% followed by tert-butyl hydroperoxide (t-BHP) treatment. The ROS levels were significantly reduced in 7^th day in each variety. In conclusion, the free radical and ROS scavenging effects were increased in female pupa with the early pupation day. The result could be used for development of bioactive materials using silkworm pupa.