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Bacteriological Characteristics of Unidentified Vibrio sp., Hemolysin Producer Isolates front Brackish Water -1. Bacteriological Characteristics of Vibrio sp., D9 (V. kumkang) Similar to V. mimicus (기수에서 분리된 용혈독소를 생산하는 미분류 Vibrio sp.의 세균학적 특징 -1. V. mimicus와 유사한 Vibrio sp. D9의 세균학적 특성)

  • KIM Young-Man;OH Hee-Kyung
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.6
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    • pp.585-590
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    • 2000
  • A hemolysin producing strain was isolated from Kum rivet estuary located in west part of Korea. In the process of identification the isolated strain was similar to V. mimicus but did not show characteristics of known Vibrio species; therefore, the strain was designated as Vibrio sp. D9 ( V. kumkang) tentatively and further identification study was carried out by comparing its bacteriological characteristics, Morphologically Vibrio sp. D9 was a typical straight roe with a polar flagellium. Among known Vibrio species no identical strains were found when using automatic bacteria identification system ($MicioLog^(TM)$system, release 4.0, Biolog Inc., USA) which evaluated the ability of metabolizing 95 kinds of carbon and nitrogen sources. Vibrio sp. D9 showed 18 and 13 different responses as compared to V. mimicus and V. cholerae, respectively. Clear hemolysis zones were observed with the strain against human and sheep blood agar plate, Hemolytic toxicity was confirmed by strong vascular permeability and fatal toxicity against mouse was also observed. Thus the strain was a pathogenic vibrio. Growth conditions for Vibrio sp. D9 were salinity of $0{\~}5.0{\%}$, pH of $6.4{\~}9.8$, temperature of $15{\~}41^{\circ}C$, respectively.

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Analysis of the wildlife distress and rescue of wild animal mammals in Gangwon province (강원도 야생동물 포유류의 조난.구조 실태분석)

  • Park, In-Chul;Lee, Ho-Won;Kim, Jong-Taek
    • Korean Journal of Veterinary Service
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    • v.35 no.1
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    • pp.33-37
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    • 2012
  • As people's interest about wildlife animal increases, more wildlife animal centers are developing. In South Korea, currently 120 mammal species live, and 22 of them are designated as endangered species. It is important to protect these natural treasures and endangered species, because they are being harmed by human industrial activity. For example, road expansion and poaching have led to the destruction of wildlife animal habitats, which is increasing year by year. Nevertheless, data regarding the systematic treatment of wild animals that are in distress is lacking, and it is a reality that analysis of such data is also lacking. The Kangwon Wildlife Rescue Center investigates these types of distress experienced by mammals. By analyzing the shape of the wild animals killed or injured, the center seeks an efficient and systematic way to manage such distress. The data collected from the Wildlife Animal Center from September 2007 to August 2010 includes 195 different cases, and the animals point of rescue. There are 16 species, including 87 water deer (Hydropotes inermis), 39 raccoons (Nyctereutes procyonoides), 20 roe deer (Capreolus pygargus bedfordi), 10 goral (Nemorhaedus goral), 7 siberian weasels (Mustela sibirica), 6 otters (Lutra lutra) and etc. Within the first year (September 2007 to August 2008), the number of mammals rescued was 46. In the second year (September 2008 to August 2009), 60 animals were rescued. in the third year (September 2009 to August 2010), the Wildlife Animal Center had 89 animals. We can see that these numbers are increasing, which shows that people are interested in the well-being of these animals because they are reporting animal injuries more and more. The mains form of distress caused by humans are from car accidents 67 (34.4%). Traps, and other hunting tools used by poachers are responsible for 25 (13.0%). Also, human industrial activity thus indirectly led to the starvation 25 (13.0%). and the orphaning of young animals 37 (19.0%).

A Study of Costume Through the Ban-cha-do in the Ka-rae-do-gam-eui-gue of the Yi Dynasty (조선시대 가례도감의궤의 반차도에 나타난 복식 연구)

  • Kim Jung Jin;Baik Young Ja
    • Journal of the Korean Society of Clothing and Textiles
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    • v.14 no.2
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    • pp.85-97
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    • 1990
  • This study investigates, through the Ka-rae-ban-cha-do(가예반차도) in the Ka-rae-do-gam­eui-gue (가예도감의궤), how the system of costume used in ceremony exchanged during the middle, late the Yi dynasty and in the period of introducing Wastern civilization. The procedure of the royal marriage is also studied here. The procedures of Ka-rae were divided into the six traditional etiguettes(륙예) : Nap-che (납채) , Nap-gyng(납징), Go-gi(고기), Chaik-bi(책비), Chin-young(친영), and Dong-roe(동뢰). The change of the general structure and characteristic of the costume used in the ceremony in Ka-rae-ban-cha-do are as follows: The costume in Ka-rae-ban-cha-do can be divided into the Guard costume (호위복식) and the Ceremonial arms costume (의장복식). The most formal attire of the Guard costume, Yangkwan-Chobok (양관-조복) and Samo -Danryeong(사모-단령) with Huygpai(흉배) can be seem only in the Ka-rae of late king's. Of the Ceremonial arms costume, the most outstanding was Hongkun- Hongeui (홍건-홍의), which showed up in almost all Ka-rae-ban-cha-do. Heukkun-Changos$\cdot$Deogrei (흑건-창옷$\cdot$더그레) were worn as well. In the style of costume emphasis convenience and practicality rather than on beauty, because they had to carry the arms used in ceremony with them nevertheless by using primary colors it was very colorful. This costume in Ka-rae-ban-cha-do has little difference, but great changes are shown in Sunjongsunjongbi Ka-rae-ban-cha-do (순종순종비가례반차도). Most costume of the past was replaced with western hat and suit, so the duplicate state of our costume and western costume came into existence during the period of introducing western civilization.

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The studies on the Chang-Pu and physiological theories of "NanJing hoeju jeonjung" ("난경회주전정(難經滙注箋正)"중(中) 장부생리(臟腑生理)에 대한 연구(硏究))

  • Je, Kang-Woo;Yoon, Chang-Yeu
    • Journal of Haehwa Medicine
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    • v.15 no.2
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    • pp.75-93
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    • 2006
  • Chang San-roe re-explained the contents from the 30th question to the 47th question of "Nan Jing" which refered to the Chang-Pu and physiological theories as follows through the western theory which wss different from the tranditioonal chinese theory 1. "Nan Jing" refered to the circulation and the coming into being of Yeong-Wi. He denied that it was classified Yeong from Wi or Eum from Yang. 2. He denied that Sam-Cho was separated to three parts and the theory of "Sam-Cho is name only, not shape", using western medical theory. 3. He denied the view about "Left side is kidney, right side is Myung-Mun" because it was incorrect that kidney was separated to two parts which were Su and Hwa or Eum and Yang 4. Re-explaining the contents of "Nan Jing"- the portions of heart and lungs, the rising and falling of liver and lungs, the relationships of five viscera and sound color smell taste fluid, the relationships of five viscera and nine hole, the relationships of five viscera and O-Chu, the physiological difference of the old and the young owing to the deflection of Yeong-Wi Chi-Heol- he thought that viewing the human body using theory of Eum and Yang, Five elements(五行) was a far-fetched interpretation, so he denied the tranditional chinese theory which understood human body's organs, physiology, pathology. 5. He explained the contents of "Nan Jing"- the portions of the colon and the small intestines, the funtion of the six bowls, the corespondence of Chang-Pu, physical peculiarity of liver, the anatomical aspects which were the sizes, weights, volumes and shapes of the Chang-Pu and the alimentary canal, Chil-Chung-Mun, Pal-Hoe-Hyeolthrough western anatomy and physiology in detail.

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Business Growth Strategy with Asset Backed Short Term Bond for Overseas IPP Opportunities (자산담보부 단기사채를 활용한 해외발전사업 수주확대방안)

  • Kim, Joon-Ho;Moon, Yoon-Jae;Lee, Jae-Heon
    • Plant Journal
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    • v.11 no.1
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    • pp.30-38
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    • 2015
  • This study is about whether the new Project Finance scheme called "Asset Backed Short Term Bond(ABSTB)" with Project Finance Guarantee Cover provided by Korean Exim Bank(KEXIM) is an appropriate and valid financing structure, through close examinations on domestic and overseas IPP case studies. This study clearly indicates that (i) the interest rate of ABSTB with KEXIM's Project Finance Guarantee is relatively more competitive than the interest rate of other ABSTB guaranteed by EPC Companies (ii) the lower credit rated EPC companies make higher ROE(Return on Equity) through this financing structure. Lastly, Korean EPC Companies can secure profitability through this innovative financing scheme which will also lead to winning more power plant Contracts and become globally competitive.

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Quality Characteristics of Low-Salt Myungran Jeotkal Fermented by Vegetable-Origin Lactic Acid Bacteria and Salt from Deep Sea Water

  • Lee, Deuk-Sik
    • The Korean Journal of Food And Nutrition
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    • v.29 no.2
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    • pp.237-245
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    • 2016
  • In this study, the physicochemical and sensory characteristics of low-salt Myungran jeotkal (Alaskan pollock roe) were evaluated after fermentation at $4^{\circ}C$ and $20^{\circ}C$ with or without the addition of deep sea water, salt from deep sea water, and vegetable-origin lactic acid bacteria (Lactobacillus fermentum JS, LBF). When fermented at $20^{\circ}C$, the addition of LBF to Myungran jeotkal resulted in a slow increase in lactic acid content, followed by an abrupt increase after five days of fermentation. However, when fermented at $4^{\circ}C$, the lactic acid content did not change significantly. Further, when Myungran jeotkal fermented at $4^{\circ}C$, the pH decreased as lactic acid production increased. The salinity of Myungran jeotkal fermented at $4^{\circ}C$ and $20^{\circ}C$ was 7% and was not affected by fermentation period. When fermented at $20^{\circ}C$, volatile basic nitrogen and amino nitrogen contents increased with increasing duration of fermentation. Further, volatile acid content decreased, however, the content of amino nitrogen increased after 11 days of fermentation with LBF and no salt effects were observed. When fermented at $20^{\circ}C$ for 13 days, preference (sensory evaluation) was the highest in all experimental groups after 9 days of fermentation, and then decreased as the fermentation period increased. The free amino acid content was highest (1,648.8 mg/100 g) in Myungran jeotkal when sun-dried salt and LBF were added, 2.3 times higher than in the control.

Effect of sequence variations within DNA melting region on the rate of formation of open complexes at $\lambdaP_{R}$ promoter ($\lambdaP_{R}$ 프로모터 열린복합체 형성에 미치는 DNA melting 부위 염기서열의 영향)

  • 정현채;노정혜
    • Korean Journal of Microbiology
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    • v.28 no.1
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    • pp.19-26
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    • 1990
  • To examine the effects of sequence variations near the transcriptional start site on the rate of formation of the open complexes at bacteriophage $\lambda P_{R}$ promoter, two mutant promoters were created by site-specific mutagenesis using synthetic oligonucleotides. Mutant I coatains changes at positions -3 and -4 from TT to CC, thus having a 6-bp long G/C stretch between -10 region and transciptional start site (+1). Mutant II has changes at positions -5 and -6 from GG to AA, thereby having a 9-bp long A/T stretch between positions -11 and -3. Selective filter binding assays were performed to measure the rate of formation of the open complexes between the wild-type or two mutant $P_{R}$ promoters on 664 bp fragments and E. coli RNA polymerase at two temperatures. At 37.deg.C, the wild-type and two mutants showed similar rates for the formation of open complex. The second order rate constant $k_{a}$ and $\tau _{int}$, as determined from the .tau.-plot analysis, were $(6.0\pm0.4)\times10^{6}M^{-1}sec^{-1}$ and $11\pm5$sec, respectively. At 18.deg.C, however, the wild-type and two mutant promoters showed differences in the kinetic parameters. k for the wild-type promoter was (2.2$\pm$0.1)\times 10^{6}M^{-1}sec^{-1}$ and $\tau _{int}$ was 76$\pm$sec. Mutant I and II exhibited differences mainly in the rate of isomerization ($\tau_{int,I}=91\pm$10 sec, int,II=34$\pm$ sec), whereas the second order rate constant $k_{a}$ was similar to the wild type value. This result implies that at $18^{\circ}C$, the isomerization rate is determined by both protein conformational change and DNA melting, which are separable kinetically according to the 3-step mechanism of Roe et al.(1984,1985), and that the base changes affected mainly the rate of DNA melting as predicted.lting as predicted.

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A Study on the Association between Sasang Constitutions and Food Preference (사상체질분류검사지(QSCC II)에 의해 사상체질이 분류된 대학생의 식품 기호도 조사 연구)

  • Choi, Sun-Mi;Chi, Sang-En;Hong, Jeong-Mi;Ahn, Kyoo-Seok;Koh, Byung-Hee
    • Korean Journal of Oriental Medicine
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    • v.7 no.1
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    • pp.105-114
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    • 2001
  • This study was carried out to investigate correlation with food preference of subjects in three constitution groups on the view point of constitutional medicine. The constitutions of 124 college students(male 80, female 44) was classified by QSCC II method. Food preference of the subjects was surveyed in accordance with suggested foods for each constitution and was analyzed using Hedonic scale. The distribution of the constitutional revealed that Soeum individuals accounts 53%, followed by ; Soyang 19%, Taeum 28%. For men, sorghum, potatoes, beef, scombroid, oyster, saury, octopus, codfish, walleye pollack, shrimp, croaker, pineapple, eggplant, leek, pumpkin, toenjang, draft beer, diluted liquor, coffee, cocoa were statistically different in food preference score by sasang constitution(p<0.05). For woman, chicken, pollack roe, a walleye pollack, persimmon, jujube, melon, citron, celery, burdock, vinegar, bean-paste soup, draft beer, ginseng, honey were statistically different in food preference score by sasang constitution(p<0.05). Association of sasang constitutions and food thought to be harmful or healthy to each constitution was analyzed. But Food preference score of food thought to be harmful or healthy were not statistically different in score among sasang constitutions. This results suggested that food intake patterns of subjects were associated with sasang constitutional food in parts.

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The Study on the Difference of Management Performance around 2009 in Korean Agricultural Cooperatives - Focused on the Result of CEO Election and Specialized Executives Director - (2009년 전후 전국 지역농협의 경영성과 차이 분석 - 조합장 교체 및 상임이사제도 운영을 중심으로 -)

  • Lee, Hyun-Chang;Park, Tae-Jun
    • Management & Information Systems Review
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    • v.35 no.4
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    • pp.21-36
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    • 2016
  • This Research focuses on the financial performance change analysis when the CEO has been changed as the consequence of the Korean Agricultural Co-op members voting. The CEO turnover is the critical event for every cooperatives because it may result in the uncertainty of management, even if top management change has a possibility to improve the management performance. To verify the CEO turnover effect for Korean Co-op financial performance, we utilized nationwide net income change data for each year before and after 2009 in this research. As a result of this effort, we find that the financially worse performance just before Co-op member voting has an positive effect on the CEO turnover. Secondary, we find a positively volatile change of net income after CEO turnover has been occurred. Specialized Executives Director system of Korean agricultural Co-ops has not the significantly positive meaning about the net income change.

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Pleiotrohpic Effect of a Gene Fragment Conferring H$_{2}$O$_{2}$ resistance in Streptomyces coelicolor

  • Um, Tae-Han;Oh, chung-Hun;Lee, Jong-Soo;Park, Yong-Doo;Roe, Jung-Hye;Kim, Jae-Heon
    • Journal of Microbiology
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    • v.33 no.4
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    • pp.339-343
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    • 1995
  • We isolated a 10 kb Bam HI fragment originated from the chromosome of a $H_2O$$^2$-resistant mutant strain of Streptomyces coelicolor, which confer $H_2O$$^2$-resistance to S. lividance upon transformation. Among various subclones ot 10kb Bam HI fragment tested for their $H_2O$$^2$-resistant phenotype in S. lividans, a subclone containing 5.2 kb Bam HI-BglII fragment was found to be responsible for $H_2O$$^2$-resistance. The plasmid containing this 5.2 kb fragment was then transformed into S. coellicolor A3(2) at early and tested for their phenotype of $H_2O$$^2$-resistance and the change in various enzymes whose activity can be stained in the gel. We found out that the 5.2 kb insert DNA conferred $H_2O$$^2$-resisstance in S. coelicolor A3(2) at early phase of cell growth. The presence of this DNA also resulted in higher level of peroxidase compared with the wild type cell containing parental vector (pIJ702) only. Esterase activity was also higher in this clone. However, alcohol dehydrogenase activity decreased compared with the wild type. These results suggest that the presence of a gene in 5.2 kb BamHI-BglII DNA fragment causes multiple changes in S. coelicolor related to its response against hydrogen peroxide. The result also implies that not only peroxidase but also esterase may function in the defencse meahsnism agianst $H_2O$$^2$-.

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