• Tunnel and Underground Space
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• v.1
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• pp.75-90
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• 1991

• Tunnel and Underground Space
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• v.6 no.1
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• pp.75-87
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• 1996

• Journal of fish pathology
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• v.36 no.2
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• pp.213-228
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• 2023

We evaluated the transcriptional response of interferon (IFN)-related genes in rock bream iridovirus (RBIV)-infected rock bream under high-, low-, or no-mortality conditions induced by different stocking water temperatures. Under the high susceptibility condition (group A, water temperature 26℃, 100% mortality), only the Mx gene was expressed early, with prolonged expression, and with heavy viral loads of approximately 10⁶~10⁷ major capsid protein gene copies/μL from 4 to 10 days post infection (dpi). However, IRF1, IRF3, IRF8, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56 were activated at later time points (8 dpi) and then quickly decreased (10 dpi). For the low susceptibility condition, the water temperature was set at 23℃ for 7 days (group B) and then reduced to 17℃. Group B exhibited a 28% mortality rate, in which persistent and effective antiviral responses were observed for long periods of time. In particular, at 20 and 22 dpi, when virus replication was peaked at approximately 10⁷/μL, the expressions of most of the IFN-related genes (IRF1, IRF3, IRF8, Mx, STAT1, ISG15, PKR, Viperin, GVIN1, IFI44, and ISG56) were significantly higher in group B than in the control group. Moreover, prolonged and higher levels of IRF3 (at least 30 dpi), IRF8 (at least 30 dpi), ISG15 (at least 30 dpi), PKR (at least 28 dpi), Viperin (at least 30 dpi), and IFI44 (at least 30 dpi) were also observed in the recovery stage of infection. Under the no-susceptibility condition at 17℃ (0% mortality), significantly elevated levels of IRF3, Mx, ISG15, and PKR were observed mostly until 20 dpi. The findings indicate that RBIV infection can induce an efficient IFN-mediated antiviral immune response in low- and no-susceptibility conditions. The findings could be valuable for effective control of viral pathogens in fish.

• Fisheries and Aquatic Sciences
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• v.13 no.1
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• pp.56-62
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• 2010

To determine whether long double-stranded RNA (dsRNA) induces RNA interference and type I interferon (IFN) responses in fish, long dsRNAs encoding enhanced green fluorescent protein (EGFP), GFPuv, and polyinosinic-polycytidylic acid sequences were co-injected with an EGFP expressing plasmid, into rock bream (Oplegnathus fasciatus). We investigated the EGFP mRNA and protein levels, and the transcriptional responses of dsRNA-dependent protein kinase and Mx1 genes. Long dsRNAs were strong inducers of a type I IFN response in rock bream, resulting in nonspecific suppression of exogenous gene expression. Furthermore, sequence-specific knockdown of exogenous gene expression at the mRNA level was detected at an early phase (24 h). These results suggested that long dsRNA may inhibit exogenous gene expression through an early mRNA interference response and a later type I IFN response in fish.

• Economic and Environmental Geology
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• v.11 no.1
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• pp.1-9
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• 1978

The study is on petrology and petrochemistry of Pajoo Acidic Igneous pluton which intruded into Precambrian metasediments of basement of the area. The geologic sequence of studied area was shown in table 1 and 10 modal analyses and 7 chemical analyses on the rock samples taken from the body. Pajoo Acidic Igneous rock consist of hypersthene-quartz-diorite and porphyritic adamellite which based on the classification of the subcommision on systematics of igneous rocks of IUGS. And porphyritic adamellite which occured as a small stock was intruded into hypersthene quartz diorite. The rock forming minerals of hypersthene quartz diorite are composed of plagioclase, perthite, quartz, hypersthene, hornblende, biotite and porphyritic adamellite is composed of perthite, quartz, plagioclase and biotite. And the former is hypidiomorphic granular texture and later is porphyritic texture with microcline phenocrysts. In silica-oxides variation diagram, the Pajoo acidic igneous rocks are similar to the trend of Daly's average composition and equivalent to the calc-alkalic rock series. In AMF diagram, these rocks are stock of fissure filling vein type by cooling in magmatic differentiation.

• Geomechanics and Engineering
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• v.18 no.1
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• pp.1-9
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• 2019

In this study, both 2D and 3D failure shapes of rock mass above the water-filled cavity are put forward when the surrounding rock mass cannot bear the pressure caused by the water-filled cavity. Based on the analytical expressions derived by kinematic approach, the profiles of active and passive failure patterns are plotted. The sensitivity analysis is conducted to explore the influences of different rock parameters on the failure profiles. During the excavation of the deep tunnels above the karst cavity, the water table always changes because of progressive failure of cavity roof. Therefore, it is meaningful to discuss the effects of varying water level on the failure patterns of horizontal rock layers. The changing laws of the scope of the failure pattern obtained in this work show good consistency with the fact, which could be used to provide a guide in engineering.

• The Journal of Engineering Geology
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• v.32 no.1
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• pp.1-12
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• 2022

Based on the extended spatial mobilization plane (SMP) criterion, we present an elastic-brittle-plastic solution for an axisymmetric cylindrical tunnel. The influences of the intermediate principal compressive stress and material strain-softening behavior are considered. Closed-form formulas for the critical support force, radius of plastic zone, and distributions of stress and displacement in surrounding rock are proposed. The elastic-plastic solution based on SMP is compared with the Kastner solution to verify the credibility of the obtained elastic-plastic solution. The elastic-brittle-plastic solution following the SMP criterion and the current solution based on the Mohr-Coulomb criterion are also compared. The rock strain-softening rate and the intermediate principal stress affect the stability of the surrounding rock. The results provide guidance for optimizing the design of support systems for tunnels.

• Tunnel and Underground Space
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• v.17 no.1 s.66
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• pp.1-8
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• 2007

In Norway, about 30 subsea tunnels have been constructed over the last 20 years. The minimum depth of 17 subsea tunnels is 56 m and rock cover are between 23 and 49 m. As the project areas for subsea tunnel are covered by water, special investigation techniques need to be applied and the investigation results are more uncertain than that of most conventional tunnel projects. The indefinite potential of water inflow and the salinic character of ingress water represent considerable problems for tunnel equipment and rock support materials. The least stable conditions are represented by major faults or weakness zones containing heavily crushed rock and clay gouge. This paper introduces the Oslofjord subsea tunnel project including minimum rock cover requirement, risk of water inflow, investigation costs, construction costs, and traffic & operation costs.

• Safety and Health at Work
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• v.6 no.4
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• pp.268-278
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• 2015

Background: This study aimed to assess the whole-body vibration (WBV) exposure among large blast hole drill machine operators with regard to the International Organization for Standardization (ISO) recommended threshold values and its association with machine- and rock-related factors and workers' individual characteristics. Methods: The study population included 28 drill machine operators who had worked in four opencast iron ore mines in eastern India. The study protocol comprised the following: measurements of WBV exposure [frequency weighted root mean square (RMS) acceleration ($m/s^2$)], machine-related data (manufacturer of machine, age of machine, seat height, thickness, and rest height) collected from mine management offices, measurements of rock hardness, uniaxial compressive strength and density, and workers' characteristics via face-to-face interviews. Results: More than 90% of the operators were exposed to a higher level WBV than the ISO upper limit and only 3.6% between the lower and upper limits, mainly in the vertical axis. Bivariate correlations revealed that potential predictors of total WBV exposure were: machine manufacturer (r = 0.453, p = 0.015), age of drill (r = 0.533, p = 0.003), and hardness of rock (r = 0.561, p = 0.002). The stepwise multiple regression model revealed that the potential predictors are age of operator (regression coefficient ${\beta}=-0.052$, standard error SE = 0.023), manufacturer (${\beta}=1.093$, SE = 0.227), rock hardness (${\beta}=0.045$, SE = 0.018), uniaxial compressive strength (${\beta}=0.027$, SE = 0.009), and density (${\beta}=-1.135$, SE = 0.235). Conclusion: Prevention should include using appropriate machines to handle rock hardness, rock uniaxial compressive strength and density, and seat improvement using ergonomic approaches such as including a suspension system.

• Geomechanics and Engineering
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• v.18 no.1
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• pp.11-20
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• 2019

The purpose of this study was to propose a new approach for quantifying in situ rock mass damage, which would include a degree-of-damage and the degraded strength of a rock mass, along with its prediction based on real-time Acoustic Emission (AE) observations. The basic approach for quantifying in-situ rock mass damage is to derive the normalized value of measured AE energy with the maximum AE energy, called the degree-of-damage in this study. With regard to estimation of the AE energy, an AE crack source location algorithm of the Wigner-Ville Distribution combined with Biot's wave dispersion model, was applied for more reliable AE crack source localization in a rock mass. In situ AE wave attenuation was also taken into account for AE energy correction in accordance with the propagation distance of an AE wave. To infer the maximum AE energy, fractal theory was used for scale-independent AE energy estimation. In addition, the Weibull model was also applied to determine statistically the AE crack size under a jointed rock mass. Subsequently, the proposed methodology was calibrated using an in situ test carried out in the Underground Research Tunnel at the Korea Atomic Energy Research Institute. This was done under a condition of controlled incremental cyclic loading, which had been performed as part of a preceding study. It was found that the inferred degree-of-damage agreed quite well with the results from the in situ test. The methodology proposed in this study can be regarded as a reasonable approach for quantifying rock mass damage.