• Journal of Wetlands Research
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• v.10 no.3
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• pp.99-109
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• 2008

This research focused on analyzing and comparing between the results of hydraulic physical modeling and the results of numerical modeling of continuous block system in river bank protection which is newly developed in-situ block system. To verify the hydraulic physical modeling and review the effectiveness, the numerical modeling was needed against the model test results for vegetation application or not. HEC-RAS model was for 1 dimensional numerical analysis and SMS was for 2 dimensional numerical analysis. The results of the two dimensional numerical simulation, under the condition of roughness coefficient calibration, show similar and rational consequence against the physical modeling. These satisfactory results show that the accomplished results of hydraulic modeling and the predicted results of numerical modeling corresponded reasonably each others.

• Journal of Convergence for Information Technology
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• v.10 no.5
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• pp.232-238
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• 2020

This study evaluated the anti-aging activity with antioxidant, anti-inflammatory and moisture activity of Ganoderma lucidum spore oil(GLS). GLS increased DPPH radical scavenging activity in a dose-dependent manners. Anti-inflammatory assay measured the inhibitory effect of GLS on NO, TNF-α and IL-6 production in LPS-stimulated RAW264.7 cells. As a result GLS inhibited NO and pro-inflammatory cytokine, TNF-α, IL-6 production. Also using human fibroblast cell to the procollagen production analysis and COL1A1 mRNA expression level analysis for defining, and for AQP-3 mRNA expression level analysis, used human keratinocyte cell. GLS increased procollagen production and COL1A1, AQP-3 mRNA expression. Our results suggest that the GLS have potential anti-inflammatory and wrinkle improves, skin moisture effect.

• Journal of Welding and Joining
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• v.20 no.4
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• pp.505-509
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• 2002

Pb is considered to be eliminated from solder, due to its toxicity. However, melting temperatures of most Pb-free solders are known higher than that of Sn37Pb. Therefore, there is a difficulty to apply Pb-free solders to electronic industry. Since Sn3Ag8Bi5In has relatively lower melting range as $188~200^{\circ}C$, on this study. Wettability and soldering characteristics of Sn3Ag8Bi5In solder in BGA were investigated to solve for what kind of problem. Zero cross time, wetting time, and equilibrium force of Sn3Ag8Bi5In solder for Cu and plated Cu such as Sn, Ni, and Au/Ni-plated on Cu were estimated. Plated Sn on Cu showed best wettability for zero cross time, wetting time and equilibrium farce. Shear strength of the reflowed joint with Sn3Ag8Bi5In ball in BGA was investigated. Diameter of the ball was 0.5mm, UBM(under bump metallurgy) was $Au(0.5\mu\textrm{m})Ni(5\mu\textrm{m})/Cu(18\mu\textrm{m})$ and flux was RMA type. For the reflow soldering, the peak reflow temperature was changed in the range of $220~250^{\circ}C$, and conveyor speed was 0.6m/min.. The shear strength of Sn3Ag8Bi5In ball showed similar level as those of Sn37Pb. The soldered balls are aged at $110^{\circ}C$ for 36days and their shear strengths were evaluated. The shear strength of Sn3Ag8Bi5In ball was increased from 480gf to 580gf by aging for 5 days.

• Proceedings of the Korea Water Resources Association Conference
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• 2010.05a
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• pp.714-718
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• 2010

하천에 설치되는 하도 내 구조물이 하천 흐름에 미치는 영향은 매우 크다고 할 수 있다. 특히 최근 한국의 4대강 살리기 사업과 관련하여 하도 준설과 동시에 하도 전체를 횡단하는 구조물이 설치되는 경우에는 하천의 수리학적 흐름특성은 더욱 크게 변화하고, 그 영향 또한 매우 광범위하게 미치게 된다. 이에 본 연구에서는 낙동강 수계에 설치 계획된 하천 횡단 구조물 1 개소를 연구 대상구간으로 선정하여 구조물 설치에 따른 수리학적 흐름 특성 변화를 분석하고자 한다. 본 연구에서는 대상 하천인 낙동강의 기 수립계획 및 관련 문헌 자료를 조사하고 금회 과업대상구간에 대해 현재 하도의 상태와 하도정비 및 보 설치계획에 따른 변화된 하도를 구분하였으며, 구분된 하도 상황에 따라 수치모형을 적용하여 결과를 분석 하였다. 하도 내 유속 및 수위 등의 기본적인 수리량은 1차원 모형의 적용으로 산정하였으며, 기 수립계획과의 비교를 통해 모형의 검증을 수행하였다. 또한, 1차원 모형이 갖는 한계 보완 및 국부적인 수리 특성 분석을 위해 2차원 동수역학 모형의 모의를 수행하였다. 이때 유량 및 기점수위 등의 경계조건은 1 2차원 모형에서 동일하게 설정하여 수치모의를 수행하였으며, 횡단 구조물 설치에 따른 주요지점의 하도 수리학적 흐름 영향 분석을 수행하였다. 각각의 수치모형의 적용 결과, 횡단 구조물 설치에 따른 하도 준설 등의 계획이 실행되면 유속 및 수위는 감소하는 것으로 분석되었으며, 유황은 하도의 중앙부에 집중되는 것으로 분석되었다. 이러한 결과는 유수의 원활한 소통을 가능케 하고, 치수 안정성 확보에 순기능을 할 것으로 판단된다. 또한, 다기능 보 즉, 하도 횡단 구조물의 설치로 인한 하도 흐름 영향 검토 결과는 고정보 부근의 흐름에서는 설치 전과 비교하여 상대적인 유속의 감소가 될 것으로 분석되었으며, 퇴적 지향적인 하도로의 변화가 예상되었다. 이와는 반대로 가 동보 부근의 흐름에서는 유속이 증가하는 것으로 분석됨에 따라 하도 본래가 갖는 안정성 확보 및 구조물의 유지관리를 위한 세심한 주의가 필요할 것으로 판단된다. 본 연구의 결과는 향후 현장 모니터링과 실내 모형실험 등의 계속된 연구 수행을 통한 자료의 축적 및 분석으로 하도 내 횡단 구조물 설치가 하도에 미치는 영향이 최소화 될 수 있는 지표가 산정될 수 있는 초석이 될 것으로 기대한다.

• Korean Journal of Microbiology
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• v.26 no.4
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• pp.305-311
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• 1988

The spectinomycin resistant strain of slow growing R. japonicum R-168 was selected to be participated in conjugation with E. coli WA803/pGS9. Tn5 was introduced from suicide vector pGS9 into R. japonicum R-168 $spr^{r}$ chromosome at the frequency of $1.0\times 10^{-5}-5.0\times 10^{-7}$ and the transconjugante were selected on the yeast extract-mannitol plate containing kanamycin ($50{\mu}$g/ml) and spectinomycin ($100{\mu}$g/ml) after 8-9 days incubation. All transconjugants we tested were found to contain Tn 5 DNA on their genome, which was confirmed by Southern hybridization experiments. R. japonicum RNa75, which had been selected through plant test, was found to be defective in symbiotic nitrogen fixing ability and the production of leghemoglobin in soybean nodules formed by the inoculation of this mutant. In addition, this mutant strain hardly developed nitrogenase activity asymbiotically in contrast with the wild type strain, indicating that some nitrogen fixing gene might be blocked in this strain and the production of leghemoglobin could be decreased by the interference in nitrogen fixing genes.

• Development and Reproduction
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• v.4 no.1
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• pp.45-52
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• 2000

Membrane type matrix metalloproteinases(MT-MMPs) have been suggested to play an important role during structural remodeling of various tissue. Expression patterns of MT1-MMP and MT2-MMP mRNAs were investigated in oocytes, embryos, ovary and oviduct of mouse during their differentiation or periovulatory period using RT-PCR technique. Both cDNA products of MT1- and MT2-MMP of immature oocytes were barely discernable with a minimum amount but the expressions were distinct in mature oocytes regardless that they were matured in vivo or in vitro. MT2-MMP was not expressed by 2-cell embryos but was expressed by 4-cell stage embryos. From the morula stage untill hatched blastocyst stage, embyos showed intesnse expression of MT2-MMP with a sudden increase at blastocyst stage. While mouse ovarian tissues showed both expression of MT1- and MT2-MMP, there was no stage-specific difference throughout the estrous cycle. Mouse oviducts also exhibit constant amount of both MT1- and MT2-MMP expressions throughout periovulatory period, i.e., before or after ovulation. These observations lead to suggest that the differential expressions of maternal MT1- and MT2-MMP during meiotic resumption of mouse oocytes and embryonic expression of MT2-MMP particularly at blastocyst stage might play a role in the differentiation of mouse oocytes and／or embryos. The precise function of MT1- and MT2-MMP with regards to their participation in the remodeling of ovarian and oviductal tissues remains in a question.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.457-461
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• 2018

Corpus luteum (CL) is a transient endocrinal tissue that undergoes repeated formation and regression during the estrous cycle. In this study, we hypothesized that the functional and structural mechanism of angiogenesis is similar between CL and tumor formation. First, we measured mRNA and protein expression of angiogenic receptors (vascular endothelial growth factor receptor-2, VEGFR2; Tie 2) in the early, middle, and late phase CL tissues during the estrous cycle. Ral-interacting protein of 76 kDa (RLIP76) and H-ras mRNA and protein were also expressed in the CL tissues. VEGFR2 and Tie 2 mRNA and protein were expressed in the early and middle phase CLs and decreased in the late phase. H-ras mRNA and protein were expressed in the early and middle phase CLs, but not in the late phase. RLIP76 mRNA was expressed in all phase CLs, and the protein expression was highest in early phase CLs. We suggest that RLIP76 and H-ras, an oncogenic gene, regulate the function of the CL during the estrous cycle, and the proteins will play an important role in the angiogenic mechanism of the CL.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.33 no.2
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• pp.103-109
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• 2000

Complete senuences of the mitochondrial rRNA Benes were determined among six salmonines in Korean Waters (Brachpmystax lenok, Onoorhpchus keta, O. masou mason, O. mason ishikawae, O. mykiss, and albino mutant of O. mykiss). The purposes of this study were to provide the basic information on levels of mtDNA polymorphism among these species for genetic characterization; discuss phylogentic relationships among three Oncorhynchus sepecies; demonstrate the utility of rRNA gene sequence data as a genetic marker for disringuishinf among Korean salmonines. PCR/direct sequencing data indicated the following consistent results; 1) 12S rRNA genes was 945 bases long in Oncorhynchus species, and 946 bases in B. lenot including one insertion. 2) Of sequence variation in mitochondrial rRNA regions, transitional substitutions were superior to transversion. 3) The significant differences were not shown in the intraspecific variation values in these gene regions. The percentage sequence divergence values were ranged from $0.066 to 0.212{\%}$. 4) The interspecific divergences were greater than the intraspecific variation. Nevertheless, ribosomal RMh genes were more conserved among species than the other mitochondrial genes, and they showed potentiality as an intergenic marker for systematics. In addition, phylogenetic trees, constructed from this data, supported that cherry salmon was closer to chum salmon than to rainbow trout, and that lenok was most distantly related species in six salmonid species.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.5
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• pp.257-269
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• 2021

This study aimed to secure basic data for the development and operation of well-aging education programs by analyzing the physical, mental, and socio-economic needs of well-aging education for successful aging. The research tool was developed as a questionnaire to investigate the perception of well aging and the needs of well-aging education in terms of physical, mental, and socio-economic aspects. In February 2021, 1949 adults over the age of 19 were surveyed through an online and mobile survey by Gallup Korea. Descriptive statistics analysis, variance analysis, Borich needs analysis, and IPA analysis were conducted to analyze the needs of well-aging education. The results revealed economic power, exercise, and chronic disease management to be high in terms of the overall priority of the education needs for well-aging, and infectious disease management, independence, and social responsibility were surveyed in the order of low education needs. In terms of economic power, education needs were highest among all age groups except for the middle-age group (35-49 years old), 82.4% of all respondents, and education needs for exercise and chronic disease management were highest in the middle-age group. Therefore, it is necessary to develop well-aging education programs for each life cycle. These results are expected to be used as empirical data in establishing a platform for developing and operating educational programs for well aging.