• BMB Reports
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• v.52 no.6
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• pp.373-378
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• 2019

The nucleotide-binding and oligomerization domain (NOD) is an innate pattern recognition receptor that recognizes pathogen- and damage-associated molecular patterns. The 29-kDa amino-terminal fibronectin fragment (29-kDa FN-f) is a matrix degradation product found in the synovial fluids of patients with osteoarthritis (OA). We investigated whether NOD2 was involved in 29-kDa FN-f-induced pro-catabolic gene expression in human chondrocytes. The expression of mRNA and protein was measured using quantitative real-time polymerase chain reaction (qrt-PCR) and Western blot analysis. Small interfering RNAs were used for knockdown of NOD2 and toll-like receptor 2 (TLR-2). An immunoprecipitation assay was performed to examine protein interactions. The NOD2 levels in human OA cartilage were much higher than in normal cartilage. NOD1 and NOD2 expression, as well as pro-inflammatory cytokines, including interleukin-1beta (IL-$1{\beta}$) and tumor necrosis factor-alpha (TNF-${\alpha}$), were upregulated by 29-kDa FN-f in human chondrocytes. NOD2 silencing showed that NOD2 was involved in the 29-kDa FN-f-induced expression of TLR-2. Expressions of IL-6, IL-8, matrix metalloproteinase (MMP)-1, -3, and -13 were also suppressed by TLR-2 knockdown. Furthermore, NOD2 and TLR-2 knockdown data demonstrated that both NOD2 and TLR-2 modulated the expressions of their adaptors, receptorinteracting protein 2 (RIP2) and myeloid differentiation 88, in 29-kDa FN-f-treated chondrocytes. 29-kDa FN-f enhanced the interaction of NOD2, RIP2 and transforming growth factor beta-activated kinase 1 (TAK1), an indispensable signaling intermediate in the TLR-2 signaling pathway, and activated nuclear factor-${\kappa}B$ (NF-${\kappa}B$), subsequently leading to increased expressions of pro-inflammatory cytokines and cartilage-degrading enzymes. These results demonstrate that 29-kDa FN-f modulated pro-catabolic responses via cross-regulation of NOD2 and TLR-2 signaling pathways.

• Journal of Korean Society of Occupational and Environmental Hygiene
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• v.20 no.4
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• pp.274-281
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• 2010

The objectives of this study are (a) to investigate the distribution patterns and exposure concentrations of biological agents in sawmill industries and (b) to compare sampling methods of biological agents. The representative processes of 5 sawmills were selected to measure total airborne bacteria, fungi, endotoxin as well as dust. Airborne bacteria and fungi were measured with one stage impactor, six stage impactor and gelatin filteration methods. Endotoxin was collected with polycarbonate filters and analysed by kinetic chromogenic Limulus Amebocyte Lysate method. Geometric mean levels of airborne bacteria, fungi, endotoxin and dust were 1,864 CFU/$m^3$, 2,252 CFU/$m^3$, 31.5 EU/$m^3$ and 2.4 mg/$m^3$. The ratios of indoor/outdoor concentrations were 3.7 for bacteria, 4.1 for fungi, 3.3 for endotoxin and 9.7 for dust. The respiratory fractions of bacteria were 68.0, 50.9, 49.2 and 45.1% in band-saw, table-saw, rip-saw process and outdoor air. The respiratory fractions of fungi were 78.7, 90.8, 87.5 and 84.8% in band-saw, table-saw, rip-saw process and outdoor air, respectively. There was no significant differences in bacterial concentrations among single stage, six stage impaction and filteration methods. But, fungal concentrations measured with filtration methods were significantly higher than those with impaction methods. Geometric mean levels of airborne bacteria and fungi were higher than the OSHA guideline values of 1,000 CFU/$m^3$. The respiratory fractions of fungi were above 75%. The concentrations of biological agents were significantly different among culture-based sampling methods. In the exposure assessments of biological agents, further studies are needed for the comparisons of diverse sampling methods and the investigations of environmental factors.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2010.05a
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• pp.304-310
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• 2010

Heat transfer coefficients in a dimpled channel, a ribbed channel, and a rip-dimple compound channel were measured by the transient liquid crystal technique. The channel aspect ratio, the rib height, the rip pitch, and the rib angle were 4:1, 6 mm, 60 mm and $60^{\circ}$, respectively. The dimple diameter and the center-to-center distance were 6mm and 7.2 mm, respectively, and the Reynolds number range was 30,000-50,000. Results showed that the heat transfer coefficients were increased by the angled rib. For the dimple-rib compound cooling cases, the heat transfer coefficients were further augmented and the thermal performance factor for the case was the highest.

• Journal of the Korean Society of Propulsion Engineers
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• v.14 no.4
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• pp.32-38
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• 2010

Heat transfer coefficients in a dimpled channel, a ribbed channel, and a rip-dimple compound channel were measured by the transient liquid crystal technique. The channel aspect ratio, the rib height, the rip pitch, and the rib angle were 4:1, 6 mm, 60 mm and $60^{\circ}$, respectively. The dimple diameter and the center-to-center distance were 6mm and 7.2 mm, respectively, and the Reynolds number range was 30,000-50,000. Results showed that the heat transfer coefficients were increased by the angled rib. For the dimple-rib compound cooling cases, the heat transfer coefficients were further augmented and the thermal performance factor for the case was the highest.

• Animal cells and systems
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• v.2 no.1
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• pp.33-47
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• 1998

Thirteen pelagic species of mysids, representing nine genera, are reported from the Korea Strait and its adjacent waters. Of these, seven species, Doxomysis quadrispinosa, Meterythrops microphthalma, Pleurerythrops secunda, Pseudanchialina pusilla, Siriella gracilis, S. okadgi, and S. thompsoni, are newly recorded from the Korean waters. A taxonomic key and illustrative figures are given.

• Journal of Korean Society for Quality Management
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• v.15 no.1
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• pp.2-12
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• 1987

Warranty cost usually involves complex factors paticularly when the product concerned consists of a great deal of parts. In spite of that it can be assessed by means of rather simple mathematical expression, it is possible to realistically appraise the sensitivity to any type of quality policy. This paper thus presents the mathematical concept related to the warranty cost and the general relationship showing that quality policy can be established in connection with the economic quality level.

• Journal of Industrial Technology
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• v.1
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• pp.3-8
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• 1981

The aim of the study is to find out the proper stage of the Korean traditional Mask Drama. For this purpose, the study investigates into the traditional Mask Dramas representative in Korea. It discusses the nature and the form of the stage of them, and through the analysis of the stage, extracts a unique elements for the stage of the traditional Mask Drama.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.457-458
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• 2001

Reproductive and life history patterns of marine amphipods are influenced by a variety of biotic and abiotic factors. Those factors may vary on local scales or along geographic patterns and result local or geographic variations in reproduction and life history. Although many studies in life history of amphipods were carried out at various habitats, limited information on the life history and reproduction at a shallow sandy shore 〈 1 m depth is available (Bear and Moore, 1996). (omitted)

• Journal of Embryo Transfer
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• v.19 no.3
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• pp.291-299
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• 2004

Characterization of quantitative trait loci (QTL) was investigated in the experimental cross population between Berkshire and Yorkshire breed. A total of 512 F$_2$ offspring from 65 matting of F$_1$ parents were phenotyped the carcass traits included average daily gain (ADG), average backfat thickness (ABF), tenth rip backfat thickness (TRF), loin eye area (LEA), and last rip backfat thickness (LRF). All animals were genotyped for 125 markers across the genome. Marker linkage maps were derived and used in QTL analysis based on line cross least squares regression interval mapping. A decision tree to identify QTL with imprinting effects was developed based on tests against the Mendelian mode of QTL expression. To set the evidence of QTL presence, empirical significance thresholds were derived at chromosome-wise and genome-wise levels using specialized permutation strategies. Significance thresholds derived by the permutation test were validated in the data set based on simulation of a pedigree and data structure similar to the Berkshire-Yorkshire population. Genome scan revealed significant evidences for 13 imprinted QTLs affecting growth and body compositions of which nine were identified to be QTL with paternally expressed inheritance mode. Four of QTLs in the loin eye area (LEA), and tenth rip backfat thickness (TRF), a maternally expressed QTL were found on chromosome 10 and 12. These results support the useful statistical models to analyse the imprinting far the QTLs related carcass trait.

• Ocean Systems Engineering
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• v.6 no.1
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• pp.23-60
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• 2016

The major objective of this study was to develop further understanding of 3D nearshore hydrodynamics under a variety of wave and tidal forcing conditions. The main tool used was a comprehensive 3D numerical model - combining the flow module of Delft3D with the WAVE solver of XBeach - of nearshore hydro- and morphodynamics that can simulate flow, sediment transport, and morphological evolution. Surf-swash zone hydrodynamics were modeled using the 3D Navier-Stokes equations, combined with various turbulence models (${\kappa}-{\varepsilon}$, ${\kappa}-L$, ATM and H-LES). Sediment transport and resulting foreshore profile changes were approximated using different sediment transport relations that consider both bed- and suspended-load transport of non-cohesive sediments. The numerical set-up was tested against field data, with good agreement found. Different numerical experiments under a range of bed characteristics and incident wave and tidal conditions were run to test the model's capability to reproduce 3D flow, wave propagation, sediment transport and morphodynamics in the nearshore at the field scale. The results were interpreted according to existing understanding of surf and swash zone processes. Our numerical experiments confirm that the angle between the crest line of the approaching wave and the shoreline defines the direction and strength of the longshore current, while the longshore current velocity varies across the nearshore zone. The model simulates the undertow, hydraulic cell and rip-current patterns generated by radiation stresses and longshore variability in wave heights. Numerical results show that a non-uniform seabed is crucial for generation of rip currents in the nearshore (when bed slope is uniform, rips are not generated). Increasing the wave height increases the peaks of eddy viscosity and TKE (turbulent kinetic energy), while increasing the tidal amplitude reduces these peaks. Wave and tide interaction has most striking effects on the foreshore profile with the formation of the intertidal bar. High values of eddy viscosity, TKE and wave set-up are spread offshore for coarser grain sizes. Beach profile steepness modifies the nearshore circulation pattern, significantly enhancing the vertical component of the flow. The local recirculation within the longshore current in the inshore region causes a transient offshore shift and strengthening of the longshore current. Overall, the analysis shows that, with reasonable hypotheses, it is possible to simulate the nearshore hydrodynamics subjected to oceanic forcing, consistent with existing understanding of this area. Part II of this work presents 3D nearshore morphodynamics induced by the tides and waves.