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Correlation of Zoysia Grass (Zoysia. spp) Survival, Reproduction, and Floret Appearance Rates to Aid in Development of New Hybrid Zoysia Grass Cultivars (잔디 교잡 품종 개발을 위한 잔디 생존률, 재생산률 및 꽃대 출현률과의 상관관계)

  • Han, Gyung Deok;Jung, Ji Hyeon;Chung, Yong Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.66 no.3
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    • pp.265-269
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    • 2021
  • This study was conducted to provide primary data through analysis of zoysia grass genetic resources to develop grass cultivars with beneficial novel properties. Zoysia grass (Zoysia. spp) is native to Korea, and is mainly propagated through stolons. However, since seed coat treatment technology was developed, the breeding of sexually reproductive grass variants has become possible, necessitating characterization of the floret appearance rate in the secured zoysia grass genetic resource before developing sexually reproductive cultivars. In this experiment, 549 grass lines were examined, revealing that florets appear in only 43 lines (7.81%). Survival rates after transplantation, and stolon generation rates displayed a significant positive correlation (Rho = 0.44). Survival rates after transfer, and rates of stolon production displayed very low correlations with floret appearance (Rho = -0.11 and Rho = -0.06). No significant results were obtained in 43 lines that displayed >20% floret appearance. To breed sexually reproductive grass variants, it is thus necessary to secure more genetic resources, considering the low rate of floret appearance. Finding traits that predict floret appearance at an early stage is also required.

Induction of the Nuclear Proto-Oncogene c-fos by the Phorbol Ester TPA and c-H-Ras

  • Kazi, Julhash U.;Soh, Jae-Won
    • Molecules and Cells
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    • v.26 no.5
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    • pp.462-467
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    • 2008
  • TPA is known to cooperate with an activated Ras oncogene in the transformation of rodent fibroblasts, but the biochemical mechanisms responsible for this effect have not been established. In the present study we used c-fos promoter-luciferase constructs as reporters, in transient transfection assays, in NIH3T3 cells to assess the mechanism of this cooperation. We found a marked synergistic interaction between TPA and a transfected v-Ha-ras oncogene in the activation of c-fos promoter and SRE. SRE has binding sites for TCF and SRF. A dominant-negative Ras (ras-N17) inhibited the TPA-Ras synergy by blocking the PKC-MAPK-TCF pathway. Dominant-negative RhoA and Rac1 (but not Cdc42Hs) inhibited the TPA-Ras synergy by blocking the Ras-Rho-SRF signaling pathway. Constitutively active $PKC{\alpha}$ and $PKC{\varepsilon}$ showed synergy with v-Ras. These results suggest that the activation of two distinct pathways such as Ras-Raf-ERK-TCF pathway and Rho-SRF pathway are responsible for the induction of c-fos by TPA and Ras in mitogenic signaling pathways.

Characteristic of Mass Transfer Volumetric Coefficient and Sauter Mean Diameter in a Liquid-Liquid Agitated Vessel (액-액 교반조내에서의 물질이동용량계수 및 액적경의 특성)

  • Lee, Young-Sei
    • Korean Chemical Engineering Research
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    • v.50 no.5
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    • pp.913-922
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    • 2012
  • Grasp of characteristics within liquid-liquid agitated vessel are very important to environment and chemical industry. Mass transfer volumetric coefficient and the Sauter mean diameter of near the droplet were measured by varying the impeller position and liquid height using the alkaline hydrolysis reaction of esters. As a result, following their good correlation was obtained. $$d_{32}=0.270\(\frac{{\sigma}^{0.6}}{{\rho}^{0.2}P^{0.4}_{Vi}}\)k_La=0.49\(\frac{6{\phi}D_A}{d^2_{32}}\)\(\frac{P_Vd^4_{32}}{{\rho}v^3}\)^{0.193}Sc^{1/3}$$.

Kinetic and Mechanism of the Addition of Benzylamines to α-Phenyl-β-thiophenylacrylonitriles in Acetonitrile

  • Hwang, Jae-young;Yang, Ki-yull;Koo, In-Sun;Sung, Dae-Dong;Lee, Ik-choon
    • Bulletin of the Korean Chemical Society
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    • v.27 no.5
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    • pp.733-738
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    • 2006
  • Nucleophilic addition reactions of p-substitutedbenzylamines $(XC _6H_4CH _2NH _2)$ to $\alpha$-phenyl-$\beta$-thiophenyl-acrylonitriles ($YC _4SH _2CH=C(CN)C_6H_4$Y') have been studied in acetonitrile at 25.0, 30.0, and 35.0 ${^{\circ}C}$. The reactions take place in single step in which the $C_\beta$ -N bond formation and proton transfer to $C_\alpha$ of $\alpha$-phenyl-$\beta$-thiophenylacrylonitriles occur concurrently with four-membered cyclic transition structure. These mechanistic conclusions are drawn based on (i) the large negative $\rho$x and large positive $\rho$Y' values and also large magnitude of $\rho$X, (ii) the negative sign and large magnitude of the cross-interaction constants ($\rho$XY), (iii) the normal kinetic isotope effects ($k_H/k_D$ > 1.0), and (iv) relatively low $\Delta H ^\neq$ and large negative $\Delta S ^\neq$ values.

Kinetic Studies on the Structure-Reactivity of Aryl Dithiomethylacetates

  • Oh, Hyuck-Keun;Park, Jie-Eun;Lee, Hai-Whang
    • Bulletin of the Korean Chemical Society
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    • v.25 no.7
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    • pp.1041-1045
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    • 2004
  • Kinetic studies of the pyridinolysis $(XC_5H_4N)$ of aryl dithiomethylacetates $(CH_3CH_2C(=S)SC_6H_4Z,\;1)$ are carried out in acetonitrile at $60.0^{\circ}C$. A biphasic Bronsted plot is obtained with a change in slope from a large $({\beta}X\;{\cong}\;0.8)$ to a small $({\beta}X\;{\cong}\;0.2)$ value at $pK_a^{\circ}$ = 5.2, which is attributed to a change in the rate limiting step from breakdown to formation of a zwitterionic tetrahedral intermediate, $T^{\pm}$, in reaction path as the basicity of the pyridine nucleophile increases. This mechanism is supported by the change of the cross-interaction constant ${\rho}xz$ from a large positive ( ${\rho}xz$ = +1.36) for the weakly basic pyridines to a small negative ( ${\rho}_xz$ = -0.22) value for the strongly basic pyridines. The magnitudes of ${\rho}z$ and activation parameters are also consistent with the proposed mechanism.

Optimal ρ acceleration parameter for the ADI iteration for the real three dimensional Helmholtz equation with nonnegative ω

  • Ma, Sangback
    • Journal of the Korean Society for Industrial and Applied Mathematics
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    • v.3 no.2
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    • pp.1-4
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    • 1999
  • The Helmholtz equation is very important in physics and engineering. However, solution of the Helmholtz equation is in general known as a very difficult phenomenon. For if the ${\omega}$ is negative, the FDM discretized linear system becomes indefinite, whose solution by iterative method requires a very clever preconditioner. In this paper we assume that ${\omega}$ is nonnegative, and determine the optimal ${\rho}$ parameter for the three dimensional ADI iteration for the Helmholtz equation. The ADI(Alternating Direction Implicit) method is also getting new attentions due to the fact that it is very suitable to the vector/parallel computers, for example, as a preconditioner to the Krylov subspace methods. However, classical ADI was developed for two dimensions, and for three dimensions it is known that its convergence behaviour is quite different from that in two dimensions. So far, in three dimensions the so-called Douglas-Rachford form of ADI was developed. It is known to converge for a relatively wide range of ${\rho}$ values but its convergence is very slow. In this paper we determine the necessary conditions of the ${\rho}$ parameter for the convergence and optimal ${\rho}$ for the three dimensional ADI iteration of the Peaceman-Rachford form for the real Helmholtz equation with nonnegative ${\omega}$. Also, we conducted some experiments which is in close agreement with our theory. This straightforward extension of Peaceman-rachford ADI into three dimensions will be useful as an iterative solver itself or as a preconditioner to the the Krylov subspace methods, such as CG(Conjugate Gradient) method or GMRES(m).

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Disruptions of Two Apparent rho-Independent Transcription Terminator Structures do not help in Enhancing the Expression of aceK in E. coli

  • Lee, Su-Ji;Chung, Taeo-Wan
    • BMB Reports
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    • v.28 no.5
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    • pp.458-463
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    • 1995
  • Two apparent rho-independent transcription terminator structures within the coding sequence of aceK have been destroyed to access their roles in the differential expression between aceA and aceK in the glyoxylate bypass operon of E. coli. The effect of mutations on the expression of aceK was evaluated in two different ways: one by maxicell labeling and the other by lacZ fusion gene construction. The maxicell labeling experiment with the mutant operon clones has failed, like that of the wild type operon clone, to visibly show isocitrate dehrogenase (IDH) kinase/phosphatase, the product of aceK, on the autoradiogram of a protein gel. When the same mutations were introduced into an aceK::lacZ fusion gene to quantitatively evaluate the mutational effect, the activity of ${\beta}-galactosidase$ in neither of the mutant versions of the fusion gene was elevated significantly enough to explain the degree of polarity observed in this region. Thus, we conclude that neither of these intragenic, apparent rho-independent transcription terminator structures, which have long been suspected as a major determinant in the down regulation of aceK, really act as a premature transcriptional terminator.

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ORTHOGONALITY IN FINSLER C*-MODULES

  • Amyari, Maryam;Hassanniah, Reyhaneh
    • Communications of the Korean Mathematical Society
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    • v.33 no.2
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    • pp.561-569
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    • 2018
  • In this paper, we introduce some notions of orthogonality in the setting of Finsler $C^*$-modules and investigate their relations with the Birkhoff-James orthogonality. Suppose that ($E,{\rho}$) and ($F,{\rho}^{\prime}$) are Finsler modules over $C^*$-algebras $\mathcal{A}$ and $\mathcal{B}$, respectively, and ${\varphi}:{\mathcal{A}}{\rightarrow}{\mathcal{B}}$ is a *-homomorphism. A map ${\Psi}:E{\rightarrow}F$ is said to be a ${\varphi}$-morphism of Finsler modules if ${\rho}^{\prime}({\Psi}(x))={\varphi}({\rho}(x))$ and ${\Psi}(ax)={\varphi}(a){\Psi}(x)$ for all $a{\in}{\mathcal{A}}$ and all $x{\in}E$. We show that each ${\varphi}$-morphism of Finsler $C^*$-modules preserves the Birkhoff-James orthogonality and conversely, each surjective linear map between Finsler $C^*$-modules preserving the Birkhoff-James orthogonality is a ${\varphi}$-morphism under certain conditions. In fact, we state a version of Wigner's theorem in the framework of Finsler $C^*$-modules.

The Fundamental Meaning of Mai and Shanghanlun Provisions Based on Paleography (『상한론(傷寒論)』 맥(脈) 및 기타 조문(條文)의 고문자학적(古文字學的) 해석(解釋)을 통한 본원적(本源的) 의미(意味) 고찰(考察))

  • Rho, Yeong-Beom;Lee, Ji-Hwan;Ha, Hyun-yee
    • 대한상한금궤의학회지
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    • v.7 no.1
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    • pp.1-14
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    • 2015
  • Objectives : The purpose of this study is to show the importance of Shanghanlun(傷寒論) paleography. Shanghanlun(傷寒論) must be thoroughly reinterpreted through graphonomy evidence. Methods : The object of this study is Gangpyeong-Shanghanlun(康平傷寒論), which is known as the oldest edition of Shanghanlun(傷寒論). This study compares the paleographic meaning of Shanghanlun(傷寒論), Mai(脈), Shanghanlun provisions 96 and 103 to existing interpretations. Results : Mai(脈) doesn't mean 'Radial pulse(Chon.Gwan.Cheok)' in Shanghanlun(傷寒論), so the meaning of Mai(脈) should be redefined. Mai(脈) means 'Continuous movement of human body' based on Chinese paleography. Also it's being verified as reasonable hypothesis in recent clinical researches. In addition, Shanghanlun(傷寒論) paleography makes it possible to grasp the true intention of Zhang Zhongjing(張仲景). Conclusions : Shanghanlun(傷寒論) paleography has a limit that it cannot be perfectly applied to clinical diagnosis. Also, it may not represent the whole meaning of Shanghanlun (傷寒論). Therefore, the value of Shanghanlun(傷寒論) reinterpretation should be recognized through this study, and we should continuously find out future values from Shanghanlun(傷寒論).

Structural Characteristics of 3- and 4-Coordinate Borons from 11B MAS NMR and Single-Crystal NMR in the Nonlinear Optical Material BiB3O6

  • Kim, Woo Young;Lim, Ae Ran
    • Journal of the Korean Magnetic Resonance Society
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    • v.17 no.1
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    • pp.24-29
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    • 2013
  • The structural characteristics of 4-coordinate $BO_4$ [B(1)] and 3-coordinate $BO_3$ [B(2)] groups in $BiB_3O_6$ were studied by $^{11}B$ magic angle spinning (MAS) and single-crystal nuclear magnetic resonance (NMR) spectroscopy. The spin-lattice relaxation time in the laboratory frame, $T_1$, for $^{11}B$ decreased slowly with increasing temperature, whereas the spin-lattice relaxation times in the rotating frame, $T_{1{\rho}}$, for B(1) and B(2), which differed from $T_1$, were nearly constant. Further, $T_{1{\rho}}$ for B(1) and B(2) showed very similar trends, although the $T_{1{\rho}}$ value of B(2) was shorter than that of B(1). The 3-coordinate $BO_3$ and 4-coordinate $BO_4$ were distinguished by $^{11}B$ MAS NMR spectrum and $T_{1{\rho}}$.