• 한국식품과학회지
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• 제42권2호
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• pp.223-232
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• 2010

면역활성을 증진시키기 위하여 수삼의 상황버섯 균사체 고체 배양을 통하여 발효물을 조제하고 조다당획분(WG-PL-CP)을 분획한 후 DEAE-Sepharose CL-6B column chromatography를 이용하여 3개의 활성 다당획분(WG-PL-CP-II, III와 IV)을 분리하였다. WG-PL-CP-III($100\;{\mu}g/mL$ 농도, saline 대조군의 1.98배)는 시료대조군인 WG-CP-III 또는 PL-CP-III(1.60배 또는 1.65배)보다 효과적인 마크로파지 활성을 나타내었고, Peyer's patch를 경유한 장관면역 활성은 WG-PL-CP-IV(1.56배)에서 관찰되었다. 한편, WG-PL-CP-II와 III(2.01배와 1.94배)는 시료대조군인 WG-CP-II와 III(1.73배와 1.66배) 또는 PL-CP-II와 III(1.79배와 1.72배)의 동일획분과 비교하여 유의적으로 증가된 마크로파지 활성을 나타내었다. 이러한 활성 획분 중 다양한 활성을 갖는 다당획분인 WG-PL-III와 IV는 주로 중성당(73.5%와 67.3%)과 산성당(23.2%와 24.6%)을 포함하는 다당류임을 알 수 있었다. 또한 구성당 분석결과, WG-PL-CP-III는 주로 산성당과 Glc, Ara, Gal, Rha와 Xyl의 구성당이 0.81:1.00:0.49:0.42:0.28:0.20의 molar ratio을 보이고 있는 반면, WG-PL-CP-IV는 산성당과 Ara, Rha, Gal, Xyl와 Glc가 1.00:0.75:0.69:0.63:0.42:0.34의 비율로 구성되었음을 나타내었다. 따라서 이러한 활성 다당획분이 수삼의 상황버섯 균사체 고체배양 발효물의 면역활성 증진에 중요하게 관여하고 있는 것으로 생각된다.

• Journal of Microbiology and Biotechnology
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• 제6권3호
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• pp.189-193
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• 1996

Submicron-sized polymeric particles (SSPP) were used to remove nucleic acids and endotoxins from cell lysates. The positively charged SSPP selectively adsorb nucleic acids and endotoxins and form complexes with them. The complexes can be easily removed by sedimentation or centrifugation. The removal of nucleic acids and endotoxins using SSPP also can be accomplished in the presence of cell and cell debris. Therefore, nucleic acids and endotoxins can be removed in an initial step of the down-stream processes. In bakers yeast and E. coli lysate systems, the level of DNA could be reduced more than three orders of magnitudes and endotoxins more than seven orders of magnitudes concurrently willi the cell debris removal process using SSPP.

• Journal of Microbiology and Biotechnology
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• 제6권5호
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• pp.352-357
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• 1996

The partition of proteins in the salt-rich phase of polyethylene glycol (pEG)/salt aqueous two-phase systems is limited by the salting-out effects of salt. The logarithm of the concentration of proteins partitioned in the salt-rich phase decreases linearly with increases in the concentration of salt in the salt-rich phase (salting-out). Therefore, the partition of a given protein in the salt-rich phase of aqueous two-phase systems can be estimated from the salting-out constant. The slope of the solubility line (salting-out con-stant) for a given protein is determined by the type of salt in the two-phase systems.

• 한국생활과학회지
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• 제12권3호
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• pp.287-301
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• 2003

The purpose of this study was to explore the relationship between childrearing belief and parental efficacy of women before and after childbirth. For this purpose 253 pregnant women and 256 mothers with infants under one year of age were contacted and asked to fill in a packet of survey questionnaire at their visits to obstetrician, gynecologist and pediatrician. The questionnaire includes questions about what mothers value in childrearing, their expectations for their children and parental efficacy. Data collected were analyzed using SAS PC program. It was reported that comparing to mothers, pregnant women view childrearing in more ideal lights and expect their children to be more exceptional. Also working mothers reported comparatively lower parental efficacy than full-time mothers suggesting that social support system to minimize pressure coming from work for working mothers with young children should be provided and the parental education methods to enhance the parental efficacy should be sought.

• 전기학회논문지
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• 제63권4호
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• pp.469-475
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• 2014

This paper deals with the characteristics evaluations of PM magnetization using stator coil in a Post-Assembly Line Start Permanent Magnet Motor (LSPMM) using a coupled Finite Element Method (FEM) and Preisach modeling, which is presented to analyze the magnetic characteristics of permanent magnets. The focus of this paper is the characteristics analysis relative to magnetizing direction and quantity of permanent magnets due to the eddy current occurring in the rotor bar during magnetization of Nd-Fe-B.

• 한국세라믹학회지
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• 제49권5호
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• pp.469-474
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• 2012

The crystallinity and phase ratio of anatase to rutile in $TiO_2$ were estimated by x-ray diffraction (XRD) and x-ray absorption spectroscopy (XAS). Traditionally, the structural characterization of $TiO_2$ powders has been carried out by XRD techniques, which are comparatively easy in use and analysis. However, materials with amorphous phase, nano-sized or nano-structured crystallinities cannot be fully characterized by XRD because XRD analysis has a limit for abnormal contributions of the nano-crystal such as the surface contribution. From the comparison with the experimental and calculated Ti K-edge XAS spectra, we found the possibility of efficient estimation in the crystalinites and the phase ratio of anatase to rutile for nano-sized $TiO_2$ mixture.

• 영어어문교육
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• 제11권4호
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• pp.209-235
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• 2005

The purpose of the present study was to investigate the effectiveness of reciprocal teaching procedure for improving Korean college students' reading comprehension of English text. In particular, this study sought to explore the qualitative features, if any, in students' use of reading comprehension strategies presented in the process of Reciprocal Reading Procedure (RRP). In order to accomplish the goal of the study, transcripts of the students' dialogues, open-ended questionnaires, and researchers' observation notes were examined. The results of the study showed that the participants used different four kinds of reading strategies in the process of RRP (questioning, clarifying, predicting, summarizing). The findings also suggested that the readers with limited knowledge of vocabulary had difficulty in moving on to the next level. Additionally, future research direction and some pedagogical implications are presented for the practical EFL classroom.

• 한국지진공학회논문집
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• 제24권6호
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• pp.243-251
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• 2020

In seismic design standards such as KDS 41 17 00 and ASCE 7, three procedures are provided to estimate seismic demands: equivalent lateral force (ELF), response spectrum analysis (RSA), and response history analysis (RHA). In this study, two steel special moment frames (SMFs) were designed with ELF and RSA, which have been commonly used in engineering practice. The collapse probabilities of the SMFs were evaluated according to FEMA P695 methodology. It was observed that collapse probabilities varied significantly in accordance with analysis procedures. SMFs designed with RSA (RSA-SMFs) had a higher probability of collapse than SMFs designed with ELF (ELF-SMFs). Furthermore, RSA-SMFs did not satisfy the target collapse probability specified in ASCE 7-16 whereas ELF-SMFs met the target probability.

• 한국조리학회지
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• 제22권6호
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• pp.24-32
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• 2016

The number of higher education institutions in Malaysia is increasing and continuously attracts a large number of international students. Therefore, it is important for foodservice operator to understand the perception of international students towards the university cafeteria in order to create student satisfaction. The aim of this study was to identify the perception of international students towards the university cafeteria, and this paper explored four research questions in order to identify the factors affecting it. The findings of this paper included a list of factors such as food quality, choices and sanitation, price, ambience and facilities and service quality of the cafeteria. This research significantly enabled the foodservice operators to gain deeper knowledge about the factors that influence the perception of international students towards the university cafeteria, which can meet the quality standards of the university.

• 대한기계학회논문집A
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• 제30권11호
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• pp.1335-1347
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• 2006

Surface energies calculated from measured contact angles between several solutions and test samples, such as Si wafer, $Al_2O_3$, $SiO_2$, PTFE(Polytertrafluoroethylene), and DLC(Diamond Like Carbon) films, based on geometric mean method and Lewis acid base method. In order to relate roughness to adhesion force, surface roughness of test samples were scanned large area and small by AFM(Atomic Force Microscopy). Roughness was representative of test samples in large scan area and comparable with AFM tip radius in small scan area. Adhesion forces between AFM tip and test samples were matched well with order of roughness rather then surface energy. When AFM tips having different radius were used to measure adhesion force on DLCI film, sharper AFM tip was, smaller adhesion force was measured. Therefore contact area was more important factor to determine adhesion force.