• 생명과학회지
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• 제17권10호
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• pp.1400-1405
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• 2007

SD계 흰쥐를 사용하여 motor activity 실험에서 동물들의 자발적인 운동성을 측정한 결과, 주어진 시간 내 움직인 시간과 움직인 거리에는 n-3 지방산이 결핍된 식이군(Def group)과 DHA가 첨가된 식이군(Def +DHA group)간에 유의적 차이를 관찰할 수가 없었다. 학습효과 실험에서 n-3 지방산이 결핍된 식이군(Def group)의 경우 목적 플래트폼까지 걸리는 시간이 DHA 첨가 식이군(Def +DHA group)에 비하여 유의적으로 길었음을 관찰할 수가 있었다(p<0.05). 수영한 거리(swimming distance)와 수영 속도(swimming speed)의 경우 수영 속도에는 유의적 차이가 없었으나 n-3 지방산이 결핍된 식이군(Def group)의 경우, DHA 첨가 식이군(Def +DHA group)에 비해 수영 풀에서 움직인 거리가 유의적으로 길었다(p<0.05). 두 식이군의 흰쥐들이 수영한 시간(swimming time)과 쉬고 있는 시간(resting time)의 경우, 쉬는 시간에는 유의적 차이가 없었으나 수영 시간 또한 n-3 지방산이 결핍된 식이(Def group)로 사육된 쥐가 DHA가 첨가된 식이로 사육된 쥐(Def +DHA group)보다 수영한 시간이 유의적으로 길었다(p<0.05). 기억력 테스트에서 n-3 지방산이 결핍된 식이군(Def group)의 경우 원래 A 지역에 대한 기억이 낮아서 A 지역을 지나가는 횟수가 다른 지역 B, C, D를 지나는 횟수와 유의적 차이가 없었다. 반면, DHA가 첨가된 식이군(Def +DHA group)의 경우 목적 플래트폼이 있었던 A 지역에 대한 기억으로 다른 지역인 B, C, D보다 지나가는 횟수가 유의적으로 많았음을 관찰하였다(p<0.05). 이상의 결과로부터 임신에서부터 성인이 될 때까지 n-3 지방산이 결핍된 식이로 사육된 쥐와 비교할 때 DHA가 첨가된 식이로 사육된 흰쥐가 Morris water maze를 이용한 공간기억력 실험에서 우수한 기억 학습효과를 나타냄을 알 수가 있었고 이러한 결과는 n-3와 n-6 지방산의 균형적인 섭취의 중요성을 부각시키고 있다.

• 생명과학회지
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• 제19권7호
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• pp.917-922
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• 2009

SD계 흰쥐를 사용하여 motor activity 실험에서 동물들의 자발적인 운동성을 측정한 결과, 주어진 시간 내 움직인 시간과 움직인 거리에는 n-3 지방산이 적절히 함유된 식이군 (Adq group)과 DHA가 첨가된 식이군(Adq+DHA group)간에 유의적 차이를 관찰할 수가 없었다. 학습효과 실험에서 n-3 지방산이 적절히 함유된 식이군 (Adq group)의 경우 목적 플랫트폼까지 걸리는 시간이 DHA 첨가 식이군(Adq+DHA group)에 비하여 다소 길었으나 유의적 차이는 관찰할 수가 없었다. 수영 속도(swimming speed)에서 DHA 첨가 식이군(Adq+DHA group)의 경우, n-3 지방산이 적절히 함유된 식이군 (Adq group)에 비해 유의적으로 빨랐으나 수영 풀에서 움직인 거리 (swimming distance)에는 두 식이군 간의 유의적 차이가 없었다. 두 식이군의 흰쥐들이 수영한 시간(swimming time)과 쉬고 있는 시간(resting time)의 경우, 쉬는 시간에는 유의적 차이가 없었으나 수영 시간 또한 n-3 지방산이 적절이 함유된 식이(Adq group)로 사육된 쥐가 DHA가 첨가된 식이로 사육된 쥐(Adq+DHA group)보다 수영한 시간이 길었으나 여기서도 유의적 차이는 없었다. 기억력 테스트에서 n-3 지방산이 적절히 함유된 식이군(Adq group) 및 DHA가 첨가된 식이군(Adq+DHA group) 모두는 목적 플랫트폼이 있었던 A 지역에 대한 기억이 우수하여 다른 지역들인 B, C, D를 지나가는 횟수보다 유의적으로 많았음을 관찰하였다(p<0.05). 이상의 결과로부터 임신에서부터 성인이 될 때까지 n-3 지방산이 적절히 함유된 식이로 사육된 쥐와 비교할 때 DHA가 첨가된 식이로 사육된 흰쥐가 Morris water maze를 이용한 공간기억력 실험에서 다소 우수한 기억 학습효과를 나타내었으나 유의적 차이는 없었음을 관찰 할 수가 있었다.

• The Korean Journal of Physiology
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• 제1권1호
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• pp.103-120
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• 1967

As pointed out by many previous investigators, the cardio-pulmonary system of well trained athletes is so adapted that they can perform a given physical exercise more efficiently as compared to non-trained persons. However, the time course of the development of these cardio-pulmonary adaptations has not been extensively studied in the past. Although the development of these training effects is undoubtedly related to the magnitude of an exercise load which is repeatedly given, it would be practical if one could maintain a good physical fitness with a minimal daily exercise. Hence, the present investigation was undertaken to study the time course of the development of cardio-pulmonary adaptations while a group of non-athletes was subjected to a daily 6 to 10 minutes running exercise for a period of 4 weeks. Six healthy male medical students (22 to 24 years old) were randomly selected as experimental subjects, and were equally divided into two groups (A and B). Both groups were subjected to the same daily running exercise (approximately 1,000 kg-m). 6 days a week for 4 weeks, but the rate of exercise was such that the group A ran on treadmill with 8.6% grade for 10 min daily at a speed of 127 m/min while the group B ran for 6 min at a speed of 200 m/min. In order to assess the effects of these physical trainings on the cardio-pulmonary system, the minute volume, the $O_2$ consumption, the $CO_2$ output and the heart rate were determined weekly while the subject was engaged in a given running exercise on treadmill (8.6% grade and 127 m/min) for a period of 5 min. In addition, the arterial blood pressure, the cardiac output, the acid-base state of arterial blood and the gas composition of arterial blood were also determined every other week in 4 subjects (2 from each group) while they were engaged in exercise on a bicycle ergometer at a rate of approximately 900 kg m/min until exhaustion. The maximal work capacity was also determined by asking the subject to engage in exercise on treadmill and ergometer until exhaustion. For the measurement of minute volume, the expired gas was collected in a Douglas bag. The $O_2$ consumption and the $CO_2$ output were subsequently computed by analysing the expired gas with a Scholander micro gas analyzer. The heart rate was calculated from the R-R interval of ECG tracings recorded by an Offner RS Dynograph. A 19 gauge Cournand needle was inserted into a brachial artery, through which arterial blood samples were taken. A Statham $P_{23}AA$ pressure transducer and a PR-7 Research Recorder were used for recording instantaneous arterial pressure. The cardiac output was measured by indicator (Cardiogreen) dilution method. The results may be summarized as follows: (1) The maximal running time on treadmill increased linearly during the 4 week training period at the end of which it increased by 2.8 to 4.6 times. In general, an increase in the maximal running time was greater when the speed was fixed at a level at which the subject was trained. The mammal exercise time on bicycle ergometer also increased linearly during the training period. (2) In carrying out a given running exercise on treadmill (8.6%grade, 127 m/min), the following changes in cardio·pulmonary functions were observed during the training period: (a) The minute volume as well as the $O_2$ consumption during steady state exercise tended to decrease progressively and showed significant reductions after 3 weeks of training. (b) The $CO_2$ production during steady state exercise showed a significant reduction within 1 week of training. (c) The heart rate during steady state exercise tended to decrease progressively and showed a significant reduction after 2 weeks of training. The reduction of heart rate following a given exercise tended to become faster by training and showed a significant change after 3 weeks. Although the resting heart rate also tended to decrease by training, no significant change was observed. (3) In rallying out a given exercise (900 kg-m/min) on a bicycle ergometer, the following change in cardio-vascular functions were observed during the training period: (3) The systolic blood pressure during steady state exercise was not affected while the diastolic blood Pressure was significantly lowered after 4 weeks of training. The resting diastolic pressure was also significantly lowered by the end of 4 weeks. (b) The cardiac output and the stroke volume during steady state exercise increased maximally within 2 weeks of training. However, the resting cardiac output was not altered while the resting stroke volume tended to increase somewhat by training. (c) The total peripheral resistance during steady state exercise was greatly lowered within 2 weeks of training. The mean circulation time during exorcise was also considerably shortened while the left heart work output during exercise increased significantly within 2 weeks. However, these functions_at rest were not altered by training. (d) Although both pH, $P_{co2}\;and\;(HCO_3-)$ of arterial plasma decreased during exercise, the magnitude of reductions became less by training. On the other hand, the $O_2$ content of arterial blood decreased during exercise before training while it tended to increase slightly after training. There was no significant alteration in these values at rest. These results indicate that cardio-pulmonary adaptations to physical training can be acquired by subjecting non-athletes to brief daily exercise routine for certain period of time. Although the time of appearance of various adaptive phenomena is not identical, it may be stated that one has to engage in daily exercise routine for at least 2 weeks for the development of significant adaptive changes.

• Fisheries and Aquatic Sciences
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• 제5권1호
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• pp.43-47
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• 2002

Jeotgal, salt- fermented sea food, is a kind of Korean traditional foods. We carried out hazard analysis on Changran (stomach and intestine of Alaska pollack) Jeotgal and squid Jeotgal through the whole processing procedures at H Co. located at Guryongpo from April 2000 to September 2001. During this studying period, we educated employee regularly and analyzed hazards on the whole process and then could conclude that pathogenic bacteria and metal particles were most important hazards in Jeotgal. Metal particles in end-product will be eliminated by laser detector before packaging. But bacteria must thoroughly manage through the whole process. Bacteriological qualities of the end products were much improved after education for the employees and by effort for HACCP (Hazard Analysis and Critical Control Point) program introduction. Pathogenic bacteria such as pathogenic Escherichia coli, Salmonella spp., coagulase positive Staphylococcus aureus and Vibrio parahaemolyticus were not detected from not only raw materials but also end products. The falling bacteria in the places such as thawing area, packaging area, seasoning area, fermenting room, subsidiary materials room and storage room were less than 30 CFU per plate for 30 minutes during working time. But those were increased more than 10 times during the resting time. It means that special measures are needed during the break time such as lunch time or exchanging working teams.

• Earthquakes and Structures
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• 제14권4호
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• pp.305-312
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• 2018

Significant research efforts were undertaken to evaluate seismic performance of vertically irregular buildings on flat ground. However, there is scarcity of study on seismic performance of buildings on hill slopes. The present study attempts to investigate seismic behaviour of reinforced concrete irregular stepback building frames with different configurations on sloping ground. Based on extensive regression study of free vibration results of four hundred seventeen frames with varying ground slope, number of story and span number, a modification is proposed to the code based empirical fundamental time period estimation formula. The modification to the fundamental time period estimation formula is a simplified function of ground slope and a newly introduced equivalent height parameter to reflect the effect of stiffness and mass irregularity. The derived empirical formula is successfully validated with various combinations of slope and framing configurations of buildings. The correlation between the predicted and the actual time period obtained from the free vibration analysis results are in good agreement. The various statistical parameters e.g., the root mean square error, coefficient of determination, standard average error generally used for validation of such regression equations also ensure the prediction capability of the proposed empirical relation with reasonable accuracy.

• Journal of Animal Science and Technology
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• 제52권2호
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• pp.149-156
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• 2010

통보리 분쇄 사료를 급여 수준별로 5개 처리구(C:무처리구, T1: 10% 첨가, T2: 20%, T3: 30%, T4: 40% 첨가)를 두고, 비육후기 5개월 동안(각 처리 구별 6두, 총 30두 공시) 처리구별로 각각 급여한 후 48시간 육안 관찰 한 그 결과를 요약하면 다음과 같다. 총 채식량(농후사료+조사료)은 T4 (9.96kg) > T1 (9.42kg) > C (9.22kg) > T3 (8.91kg) > T2 (8.51kg) 순으로 높게 나타났다(P<0.05). 채식시간은 T4 > T3 > C > T1 > T2구 순이였으며 (P<0.01), 반추시간은 T4 > T1 > C > T3 > T2구 순으로 나타났다 (P<0.05). 그러나 휴식시간은 반추시간과 반대로 T2 > T1 > C > T3 > T4 순으로 나타났다(P<0.01). 채식시간과 반추시간을 합한 저작 시간은 T4 (392.5 min.) > T3 (331.4 min.) > C (329.4 min) > T1 (327.2 min) > T2 (276.3 min.) 순으로 나타났다(P<0.01). 식괴수, 총 저작수, 식괴당 저작수, 사료가치지수는 각각 190.9개, 9,129.4회, 47.8회, 39.4 (분/kg)으로 T4구가 가장 높게 나타났다(P<0.01). 식괴당 반추시간은 C구가 가장 높고 T3구가 가장 낮게 나타났으며(P<0.05), 분당 식괴수는 T3 가장 높고 C구가 가장 낮게 나타났다(P<0.05). 채식율과 저작효율은 T2구에서 가장 높았지만(P<0.01, 0.05), 반추효율에서는 T1구가 가장 높았다. 그리고 군 행동에서는 휴식(78.5%) > 반추(11.4%) > 채식(10.1%)순으로, 군 행동 중 서서 휴식은 T3 > C > T2 >T1 >T4 구 순으로 높게 나타났다.

• 한국수정란이식학회지
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• 제22권3호
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• pp.185-190
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• 2007

본 연구는 젖소 경산우와 미경산우의 다양한 발정 행동 특성과 배란 시간을 조사하기 위해 수행되었다. 공시된 89두 중 73두에서 배란이 일어났으며, 이 중 61두에서 발정 발현이 나타났다. 다양한 발정 행동은 $PGF_2{\alpha}$ 투여 후 2일째부터 72시간 동안 육안 관찰하였으며, 배란 시간(4시간 간격으로 초음파 시험)과의 관계를 각각 조사하였다. 경산우의 발정 발현율은 외음부 냄새 맡기, 턱 비빔, 승가 및 승가 허용에서 각각 81%, 78%, 78% 및 56%로 나타났으며, 미경산우에서는 외음부 냄새 맡기, 턱 비빔, 승가 및 승가 허용은 각각 61%, 68%, 82% 및 76%를 보였다. 젖소 경산우와 미경산우에서 발정 개시 후 배란까지의 평균 $25.58{\pm}7.94$와 $25.55{\pm}5.72$시간이었으며, 발정 종료 후 배란까지는 평균 $13.42{\pm}7.14$와 $7.48{\pm}7.4$시간이었다. 경산우의 발정 개시 후 배란까지의 시간에서는 승가 허용($17.33{\pm}5.83$시간)이 승가, 외음부 냄새 맡기, 턱 비빔($23.58{\pm}5.12$시간, $24.25{\pm}6.09$시간, $23.42{\pm}6.04$시간)보다 유의적으로(p<0.05) 짧게 나타났으며, 미경산우에서는 유의적인 차이가 인정되지 않았다. 승가 허용 종료에서 배란까지의 시간에서는 미경산우($6.38{\pm}4.80$)가 경산우($13.05{\pm}4.53$)에 비해 유의적으로(p<0.05) 짧게 나타났다. 본 연구 결과에 의해 우유생산에 관련한 생리적인 변화가 젖소 발정행동과 배란 특성의 변화의 주요 요인일 수 있다고 사료된다.

• Journal of Oral Medicine and Pain
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• 제21권1호
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• pp.89-101
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• 1996

This study was performed to investigate influence of the changes of head posture on resting electromyographic (EMG) activity in anterior temporalis, masseter, sternocleidomastoid muscle and trapezius, and on status of occlusal contacts. For this study twenty-nine patients with temporomandibular disorders(TMD) and thirty dental students without any masticatory symptoms were selected as patients group and control group, respectively. EMG activity($\mu$V) at rest was observed in four kind of head postures such as natural or normal head posture(NHP), forward head posture(FHP), upward head posture(UHP), downward head posture(DHP), and in NHP and FHP, EMG activity with flat occlusal splint was also checked. BioEMG$^\textregistered$(Bioelectromyograph, Bioresearch Inc., USA) was used to record EMG activity in the above four muscles with eight locations on both sides. The author used T-Scan$^\textregistered$(Tekscan Co., USA) system to investigate the changes of oclusal contats on clenching in the four head postures about number, force, time(duration) and total left-right statistis(TLR, occlusal stability crossing left-right dental arch on clenching). For taking in upward or downward head posture, head was inclined $10^{\circ}$ upward or downward and CROM$^\textregistered$ (cervical-range-of motion, Performance attainment Inc., USA) was used to maintain same posture during the procedure. The results obtained were as follows : 1. For resting EMG activity, anterior temporalis did not show any difference by change of head posture, but masseter and sternocleidomastoid muscle showed higher value of EMG activity in FHP and UHP, and trapezius showed higher value of EMG activity in FHP and DHP. 2. EMG activity of trapezius was higher than that of any other muscles in NHP, FHP, and DHP, but in UHP, the activity was the lowest reversely. 3. Patients group showed higher EMG activity than control group did in all the muscles in NHP. And significant difference between the two groups were also observed in anterior temporalis in FHP, in sternocleidomastoid muscle in UHP, and in sterno-cleidomastoid muscle and trapezius in DHP with higher activity in patients group. 4. There was no change of EMG activity in NHp with splint, but EMG activity in anterior temporalis and masseter was decreased in FHP with splint. 5. In general, status of occlusal contacts was not changed with head posture in all subjects, and difference between patients group and control group was only noted for number and force of tooth contact in UHP and DHP with more value in control group. 6. Correlationship between EMG activity and number ad force of tooth contacts was shown negatively with regard to masseter in NHP, and trapezius in UHP and DHP.

• 대한물리치료과학회지
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• 제13권2호
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• pp.67-83
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• 2006

The present study examined that in Vivo/Vitro test is investigated in normotensive sham-operated rats (NSR) and aldosterone-analogue deoxycorticosterone acetate (DOCA)-salt hypertensive rats (ADHR) and that the antihypertensive effect was induced by silver spike point (SSP) electrical stimulation at meridian points(CV-3, -4, Ki-12, SP-6, LR-3, BL-25, -28, -32, -52), specifically, such as aldosterone in 24 hour urine analysis from healthy volunteer. The gross examination and morphometric-histological changes, such as hypertrophy, production of necrotic tissues, and the changes of cell arrangement on the kidney, and adrenal gland were markedly observed in aldosterone-analogue DOCA-salt hypertensive rats compared with those from normotensive sham-operated rats. The systolic blood pressure, weight of kidney and adrenal gland were significantly increased in ADHR than that in NSR. The required time of PSS-induced resting tone was significantly increased in ADHR than that in NSR. However, the voltage-dependent K+ (Kv) currents were significantly decreased in ADHR than that in NSR. The urine analysis showed that the concentration of aldosterone was significantly decreased in resting state from the elderly people compared with those from the adolescent healthy volunteer. The current of 1 Hz continue type of SSP electrical stimulation significantly decreased in the concentration of aldosterone of 24 hour urine from the elderly people. These results suggest that the development of aldosterone analogue-induced hypertension is associated with changed the weight of kidney and adrenal gland, blood pressure, resting tone and Kv currents, which directly affects blood pressure. Therefore, the hypertension is a risk factor on cerebrovascular disease. Moreover, these results suggest that the diminished responsiveness to SSP electrical stimulation, especially current of 1Hz continue type, in elderly people may be, in part, related by the increased of antihypertensive effects.

• Annals of Clinical Neurophysiology
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• 제8권1호
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• pp.29-35
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• 2006

Background: Transcranial magnetic stimulation (TMS) is a non-invasive diagnostic method particularly suited to investigation the long motor tracts. The clinical value of TMS in most spinal cord diseases has still to be made. Diagnostic value of magnetic motor evoked potential (MEP) parameters in intramedullary spinal cord lesions was investigated. Methods: MEP elicited by TMS was recorded in 57 patients with clinically and radiologically defined intramedullary myelopathy. Twenty five patients with cervical myelopathy (CM) and 32 thoracic myelopathy (TM) were included. Recordings were performed during resting and minimal voluntary contraction at both abductor pollicis brevis (APB) and tibialis anterior (TA) muscles. Stimulation threshold(ST), amplitude, and central motor conduction time (CCT) were measured at resting and facilitated conditions. CCT was calculated by two means; central motor latency (CML)-M using magnetic transcranial and root stimulation, and CML-F using electrical F-wave study. The results were compared between patient groups and 10 normal control group. Results: Facilitated mean ST recorded at TA was elevated in both CM and TM compared with control group. Resting mean CML-M at TA was significantly prolonged in both CM and TM, and CML-M was absent or delayed in 37.1% of CM and 8% of TM at APB with facilitation. Facilitated mean MEP amplitude at ABP was lower in CM than in TM, while MEP/M ratios were not different significantly between groups. Conclusions: Magnetic motor evoked potential has diagnostic value in intramedullary myelopathy and localizing value in differentiating between CM and TM by recording at APB and TA. It is a noninvasive way to investigate the functional status of motor tracts of spinal cord.