• 제목/요약/키워드: RE(Reverse engineering)

검색결과 73건 처리시간 0.026초

Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method

  • Jonson, Miranda Gilda;Lian, Sen;Choi, Hong-Soo;Lee, Gwan-Seok;Kim, Chang-Suk;Kim, Kook-Hyung
    • The Plant Pathology Journal
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    • 제27권2호
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    • pp.148-155
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    • 2011
  • Our previous sequence and phylogenetic analyses of the Korean Rice stripe virus (RSV) suggested possible genetic reassortment of RNA segments, but whether this RNA variation contributed to the recent RSV outbreaks in Korea is yet unclear. To further clarify these RSV-RNA segment variations, we developed a reverse transcription-polymerase reaction/restriction enzyme (RT-PCR/RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV RNA 1-4 subtypes, respectively. Our RT-PCR/RE results provided a clear pattern of RNA reassortment, i.e., different groups of isolates having their RNA segments derived from two to three different RSV ancestors, such as from Eastern and Southwestern Chinese or Japanese M and T isolates. We also found that the migratory small brown planthopper from Eastern China caught by aerial net traps that possesses RSV-RNA3 genotypes corresponds mainly to Eastern China, with a few for Southwestern China based on RT-PCR/RE, sequence and phylogenetic analyses, indicating that RSV populations in Eastern China may also have strong RNA variation. The development of an RE analysisbased method proved a useful epidemiological tool for rapid genotyping and identification of mixed infections by RSV strain and by different subtype.

Numerical investigation of supercavity geometry and gas leakage behavior for the ventilated supercavities with the twin-vortex and the re-entrant jet modes

  • Xu, Haiyu;Luo, Kai;Dang, Jianjun;Li, Daijin;Huang, Chuang
    • International Journal of Naval Architecture and Ocean Engineering
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    • 제13권1호
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    • pp.628-640
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    • 2021
  • To investigate the supercavity geometry and gas flow structure for the supercavities with two closure types under the different flow conditions, an inhomogeneous multiphase model with the SST turbulence model was established, and validated by experimental results. The results show that two distinct regions exist inside the supercavity, which include the downstream flow region along the gas-water interface and the reverse flow region. For the twin-vortex supercavity, the internal gas leaks from the supercavity boundary by two paths: the supercavity surface and the two-vortex tubes. Increasing Froude number leads to more internal gas stripped from the supercavity surface. Two types of gas loss exist for the re-entrant jet supercavity with high Froude number, one type is the steady process of gas loss, and the major gas-leaking path is the supercavity surface rather than supercavity closure region. The other type is the unsteady periodic ejection, and the gas cluster of periodic ejection is merely a small part of the gas stored inside the supercavity.

Development Process of FPGA-based Departure from Nucleate Boiling Ratio Algorithm Using Systems Engineering Approach

  • Hwang, In Sok;Jung, Jae Cheon
    • 시스템엔지니어링학술지
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    • 제14권2호
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    • pp.41-48
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    • 2018
  • This paper describes the systems engineering development process for the Departure from Nucleate Boiling Ratio (DNBR) algorithm using FPGA. Current Core Protection Calculator System (CPCS) requirement and DNBR logic are analyzed in the reverse engineering phase and the new FPGA based DNBR algorithm is designed in the re-engineering phase. FPGA based DNBR algorithm is developed by VHSIC Hardware Description Language (VHDL) in the implementation phase and VHDL DNBR software is verified in the software Verification & Validation phase. Test cases are developed to perform the software module test for VHDL software modules. The APR 1400 simulator is used to collect the inputs data in 100%, 75%, and 50% reactor power condition. Test input signals are injected to the software modules following test case tables and output signals are compared with the expected test value. Minimum DNBR value from developed DNBR algorithm is validated by KEPCO E&C CPCS development facility. This paper summarizes the process to develop the FPGA-based DNBR calculation algorithm using systems engineering approach.

Flow structures around a three-dimensional rectangular body with ground effect

  • Gurlek, Cahit;Sahin, Besir;Ozalp, Coskun;Akilli, Huseyin
    • Wind and Structures
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    • 제11권5호
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    • pp.345-359
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    • 2008
  • An experimental investigation of the flow over the rectangular body located in close proximity to a ground board was reported using the particle image velocimetry (PIV) technique. The present experiments were conducted in a closed-loop open surface water channel with the Reynolds number, $Re_H=1.2{\times}10^4$ based on the model height. In addition to the PIV measurements, flow visualization studies were also carried out. The PIV technique provided instantaneous and time-averaged velocity vectors map, vorticity contours, streamline topology and turbulent quantities at various locations in the near wake. In the vertical symmetry plane, the upperbody flow is separated from the sharp top leading edge of the model and formed a large reverse flow region on the upper surface of the model. The flow structure downstream of the model has asymmetric double vortices. In the horizontal symmetry plane, identical separated flow regions occur on both vertical side walls and a pair of primary recirculatory bubbles dominates the wake region.

3차원 형상 스캔의 측정방법 개선에 관한 연구 (A Study on Improvement in a Method of Three Dimensional Configuration Scan Measurement)

  • 김태호;김민주;이승수;박정보;전언찬
    • 한국공작기계학회:학술대회논문집
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    • 한국공작기계학회 2001년도 추계학술대회(한국공작기계학회)
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    • pp.375-380
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    • 2001
  • This study is on the precision of the optical scanner which is suitable for shape modeling and non-contact equipment, comparing with a razer scanner. The optical scanner not only has all merits that non-contact methods have but also improve a veil phenomenon which is a razer scanner's demerits. However, the optical scanner has not been used extensively because the measurements are not very precise and there are not the definite methods of measurement. Hence, this study is to find out how parameters such as camera's hight, angle, luminous intensity, distance to object, and so on have an influence on measuring using the optical scanner and to establish the methods of measuring precision.

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광학식 스케너의 측정방법 개선에 관한 연구 (A Study on Improvement of the Measurement Method for Optical Scanner)

  • 주만식;김민주;이승수;김순경;전언찬
    • 대한기계학회:학술대회논문집
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    • 대한기계학회 2001년도 추계학술대회논문집A
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    • pp.841-847
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    • 2001
  • This study is on the precision of the optical scanner which is suitable for shape modeling and non-contact equipment, comparing with a razer scanner. The optical scanner not only has all merits that non-contact methods have but also improve a veil phenomenon which is a razer scanner's demerits. However, the optical scanner has not been used extensively because the measurements are not very precise and there are not the definite methods of measurement. Hence, this study is to find out how parameters such as camera's hight, angle, luminous intensity, distance to object, and so on have an influence on measuring using the optical scanner and to establish the methods of measuring precision.

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축열조 성능에 미치는 디퓨저 형상의 영향 (Effect of Diffuser Shape on the Performance of Water-Chilled Heat Storage)

  • 정재동;박주혁;조성환
    • 설비공학논문집
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    • 제16권4호
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    • pp.374-382
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    • 2004
  • Diffuser design is crucial for water-chilled heat storage. Its impact on the system performance is more significant for the underground tank due to inherent limit on the aspect ratio and tank shape. The effect of diffuser shape on the performance of the water-chilled heat storage is numerically investigated. Three dimensional simulation has been conducted for fully incorporating the complex diffuser shape and the non-symmetric tank shape. Mixing at the inlet of the diffuser depends on the inlet Reynolds number, Froude number and the diffuser shape. Three types of the diffuser shape and the broad range of Reynolds number (Re=400, 800, 1200) and Froude number (Fr=0.5, 1.0, 2.0) are examined. The performance of the heat storage tank is evaluated by the thermocline thickness which is reverse to the degree of stratification. The radial regulated plate diffuser, which is the suggested diffuser shape in this study, shows the lowest thermocline thickness in the condition considered.

서브 프레임 제작용 다목적 용접지그 개발 (Development of Multipurpose Welding Jig for Sub-Frame)

  • 신형곤
    • 한국기계기술학회지
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    • 제13권4호
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    • pp.131-136
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    • 2011
  • In commercial vehicle, sub-frame which equipped in main frame supporting dump deck and oil tanker. This is the main structure for all equipment which including joint function. Sub-frame is made by welding process, this susceptible to deform and crack by its longitudinal size. Also various kind of sub-frame make it difficult to standardization in manufacturing process and exclusive jig is not adapted yet. Frame size is over 6~8m and weight is more than 300kg this make re-work more difficult. If manufacturing company made precise sub-frame, this is not only convenient for customers but also save the company money by reducing the working time. In this study manufacture the sub-frame be suitable for its main function and develop exclusive welding jig for obtain checking fixture function as well.

랜덤 패턴 투영을 이용한 스테레오 비전 시스템 기반 3차원 기하모델 생성 (3D geometric model generation based on a stereo vision system using random pattern projection)

  • 나상욱;손정수;박형준
    • 한국경영과학회:학술대회논문집
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    • 한국경영과학회/대한산업공학회 2005년도 춘계공동학술대회 발표논문
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    • pp.848-853
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    • 2005
  • 3D geometric modeling of an object of interest has been intensively investigated in many fields including CAD/CAM and computer graphics. Traditionally, CAD and geometric modeling tools are widely used to create geometric models that have nearly the same shape of 3D real objects or satisfy designers intent. Recently, with the help of the reverse engineering (RE) technology, we can easily acquire 3D point data from the objects and create 3D geometric models that perfectly fit the scanned data more easily and fast. In this paper, we present 3D geometric model generation based on a stereo vision system (SVS) using random pattern projection. A triangular mesh is considered as the resulting geometric model. In order to obtain reasonable results with the SVS-based geometric model generation, we deal with many steps including camera calibration, stereo matching, scanning from multiple views, noise handling, registration, and triangular mesh generation. To acquire reliable stere matching, we project random patterns onto the object. With experiments using various random patterns, we propose several tips helpful for the quality of the results. Some examples are given to show their usefulness.

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유기물 농도가 낮은 고품질 정수 생산을 위한 고압막여과 공정 설계 시 고려사항 (Considerations to design high-pressure membrane system to produce high quality potable water with lower organic matter concentration)

  • 전종민;김성수;서인석;김수한
    • 상하수도학회지
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    • 제34권6호
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    • pp.473-480
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    • 2020
  • High-pressure membrane system like nanofiltration(NF) and reverse osmosis(RO) was investigated as a part of water treatment processes to produce high quality potable water with low organic matter concentration through membrane module tests and design simulation. River water and sand filtration permeate in Busan D water treatment plant were selected as feed water, and NE4040-90 and RE4040-Fen(Toray Chemical Korea) were used as NF and RO membranes, respectively. Total organic carbon(TOC) concentrations of NF and RO permeates were mostly below 0.5 mg/l and the average TOC removal rates of NF and RO membranes were 93.99% and 94.28%, respectively, which means NF used in this study is competitive with RO in terms of organic matter removal ability. Different from ions rejection tendency, the TOC removal rate increases at higher recovery rates, which is because the portion of higher molecular weight materials in the concentrated raw water with increasing recovery rate increases. Discharge of NF/RO concentrates to rivers may not be acceptable because the increased TDS concentration of the concentrates can harm the river eco-system. Thus, the idea of using NF/RO concentrate as the raw water for industrial water production was introduced. The design simulation results with feed water and membranes used in this work reveal that the raw water guideline can be satisfied if the recovery rate of NF/RO system is designed below 80%.