• Proceedings of the Korean Institute of Building Construction Conference
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• 2017.11a
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• pp.139-140
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• 2017

This technology employs a method of forming a single-ply PLUS waterproofing sheet layer comprised of applying a single-ply synthetic polymer layer on a vibrating structure (steel frame, RC) or an inclined surface by using a T joint lap-filling coil and an embedded metal coated sheet. The T - joint reinforcing lap-filling coil was used to block the ingress channel of the rainwater by applying the material in the vulnerable area where the three sides of the waterproof sheet overlapped. Conventional waterproofing techniques have a problem in that the waterproof sheet is pierced because the end portion of the waterproof sheet applied to the vertical portion is fixed by a nail, and the sealant applied to the end portion of the sheet cannot easily secure long-term waterproof durability due to the influence of the external environment. Therefore, the developed technology secured the waterproof durability against the vertical part by using the embedded metal sheet. In addition, automatic hot-air fusing is used to improve the quality of waterproof construction and point fixation method using fixed hardware. This is a technology that is not significantly restricted in the high degradation level regions of domestic waterproof construction environments in Korea such as low-temperature environment, wet floor.

• Journal of the Architectural Institute of Korea Structure & Construction
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• v.36 no.4
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• pp.153-161
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• 2020

This study investigates the recycling potential of demolition waste (DW) according to building structure, while considering economic aspects. For that, this study surveyed 1,034 residential buildings to collect reliable information on demolition waste generation rates (DWGRs). This study suggested a method for operational cost calculation for each stage and carried out an inventory analysis. The economic value of recycled DW materials was also calculated. And then, the recycling potential(RP) was calculated by building structures and waste types. RP by building structure was low (27-40%), and RP was found in the order of masonry-block, wooden, RC and concrete-brick. By type of DWs, the RP of aggregates was considerably lower than 7%, and DWs such as wood, plastics, and metals showed more than 100% RP. Considering the results of this study, In order to improve the RP of buildings and DWs, the diversification of products that recycled waste like aggregates (i.e., mortar, concrete, bricks, blocks, tiles) and the development of high value-added products are considered to be the most urgent problems. Based on the above RP results, this study proposed a more advanced method for life cycle assessment of buildings and demolition waste.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.28 no.2
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• pp.311-326
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• 2018

This paper has experimented using News sample video with QCIF ($176{\times}144$) resolution in JM v10.2 code of H.264/AVC-MPEG. The region of interest (ROI) to be encrypted occurred the drift by unnecessarily referring to each frame continuously in accordance with the characteristics of the motion prediction and compensation of the H.264 standard. In order to mitigate the drift, the latest related research method of re-inserting encrypted I-picture into a certain period leads to an increase in the amount of additional computation that becomes the factor increasing the bit-rate overhead of the entire video. Therefore, the reference search range of the block and the frame in the ROI to be encrypted is restricted in the motion prediction and compensation for each frame, and the reference search range in the non-ROI not to be encrypted is not restricted to maintain the normal encoding efficiency. In this way, after encoding the video with restricted reference search range, this article proposes a method of RC4 bit-stream encryption for the ROI such as the face to be able to identify in order to protect personal information in the video. Also, it is compared and analyzed the experimental results after implementing the unencrypted original video, the latest related research method, and the proposed method in the condition of the same environment. In contrast to the latest related research method, the bit-rate overhead of the proposed method is 2.35% higher than that of the original video and 14.93% lower than that of the latest related method, while mitigating temporal drift through the proposed method. These improved results have verified by experiments of this study.

• Research in Plant Disease
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• v.21 no.4
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• pp.315-320
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• 2015

Soybean mosaic virus (SMV) is a prevalent pathogen that causes significant yield reduction in soybean production worldwide. SMV belongs to potyvirus and causes typical symptoms such as mild mosaic, mosaic and necrosis. SMV is seed-borne and also transmitted by aphid. Eleven SMV strains, G1 to G7, G5H, G6H, G7H, and G7a were reported in soybean varieties in Korea. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method allowed one-step detection of gene amplification by simple procedure and needed only a simple incubator for isothermal template. This RT-LAMP method allowed direct detection of RNA from virus-infected plants without thermal cycling and gel electrophoresis. In this study, we designed RT-LAMP primers named SML-F3/B3/FIP/BIP from coat protein gene sequence of SMV. After the reaction of RT-LAMP, products were identified by electrophoresis and with the detective fluorescent dye, SYBR Green I under daylight and UV light. Optimal reaction condition was at $58^{\circ}C$ for 60 min and the primers of RT-LAMP showed the specificity for nine SMV strains tested in this study.

• Journal of Microbiology and Biotechnology
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• v.25 no.7
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• pp.1170-1176
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• 2015

Ginsenosides, the major active component of ginseng, are traditionally used to treat various diseases, including cancer, inflammation, and obesity. Among these, compound K (CK), an intestinal bacterial metabolite of the ginsenosides Rb₁, Rb₂, and Rc from Bacteroides JY-6, is reported to inhibit cancer cell growth by inducing cell-cycle arrest or cell death, including apoptosis and necrosis. However, the precise effect of CK on breast cancer cells remains unclear. MCF-7 cells were treated with CK ($0-70{\mu}M$) for 24 or 48 h. Cell proliferation and death were evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry assays, respectively. Changes in downstream signaling molecules involved in cell death, including glycogen synthase kinase $3\beta$ ($GSK3\beta$), $GSK3\beta$, $\beta$-catenin, and cyclin D1, were analyzed by western blot assay. To block $GSK3\beta$ signaling, MCF-7 cells were pretreated with $GSK3\beta$ inhibitors 1 h prior to CK treatment. Cell death and the expression of $\beta$-catenin and cyclin D1 were then examined. CK dose- and time-dependently inhibited MCF-7 cell proliferation. Interestingly, CK induced programmed necrosis, but not apoptosis, via the $GSK3\beta$ signaling pathway in MCF-7 cells. CK inhibited $GSK3\beta$ phosphorylation, thereby suppressing the expression of $\beta$-catenin and cyclin D1. Our results suggest that CK induces programmed necrosis in MCF-7 breast cancer cells via the $GSK3\beta$ signaling pathway.

• The Journal of the Convergence on Culture Technology
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• v.8 no.6
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• pp.667-674
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• 2022

In this study, the correlation between the damage type and operating conditions of the sleepers was analyzed based on the design data and visual inspection results for the concrete sleepers of the sleeper floating track (STEDEF) that have been in operation for more than 20 years. It appeared in the form of cracks, breakages, and breaks in the concrete at the center and tie bar contact and buried areas. As a result of the numerical analysis, it was analyzed that the change in the left and right spring stiffness of the sleeper resilience pad increases the maximum stress, tensile stress, compressive stress, and displacement of the concrete sleeper, and stress concentration in the concrete at the tie bar contact area. It was proved analytically that the sleeper resilience pad can affect the damage of the concrete sleeper. Therefore, damage of concrete sleepers in the sleeper floating track in urban transit could be caused by changes in spring stiffness of sleeper resilience pads. It was reviewed that preventive maintenance such as improvement and timely replacement of sleeper resilience pads was necessary.