• 대한의생명과학회지
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• 제25권2호
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• pp.190-195
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• 2019

Hemoglobin (Hb) concentrations and hematocrit (Hct) values can be changed by factors such as erythrocyte production, destruction, and bleeding. In addition, variants in the protein expression involved in the amount of red blood cells that determine Hb metabolism or Hct value can increase susceptibility to complex blood diseases. Previous studies have reported significant single nucleotide polymorphisms (SNPs) by applying a genome-wide association study (GWAS) on Hb levels and Hct values in European population. In this study, we confirmed whether the significant SNPs are replicated in Koreans. In previous studies, 26 and 18 SNPs with a significant correlation Hb and Hct were identified in Korean genotype data, and 21 and 12 SNPs were selected, respectively. The SNPs of PRKCE (rs10495928), TMPRSS6 (rs2235321, rs5756505, rs855791) were significantly associated with Hb (P<0.05). In the association analysis of Hct, the SNPs of HBS1L (rs6920211, rs9389268, rs9483788), PRKCE (rs4953318), SCGN (rs9348689) and TMPRSS6 (rs2413450) genes showed a significant correlation (P<0.05). Replicated SNPs and not replicated SNPs showed the difference of genetic distance calculated by Fst. The replicated SNPs with a significant correlation showed similar allele frequencies, whereas the not replicated SNPs showed a large difference in allele frequency. All replicated SNPs with significant correlations had Fst values less than 0.05, indicating that the genetic distance between the groups was close. On the other hand, the not replicated SNPs showed that the Fst value was 0.05 or more and the genetic distance was relatively large.

• 한국어병학회지
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• 제26권2호
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• pp.89-97
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• 2013

크롬은 여러 산업으로 배출되어 수 환경을 오염시키는 주요 중금속 중 하나이며, 특히 Cr(VI)은 수용액 내에서 주로 크롬산 이온으로 존재하며, 생체막을 쉽게 통과하여 산화 스트레스 및 생체 축적을 일으킨다. 따라서 본 연구는 숭어를 대상으로 그들의 혈액성분의 변동에 미치는 크롬의 영향을 검토하였다. RBC count 및 Ht 값은 $50{\mu}g/L$ Cr 농도에서 유의적인 감소를 나타냈으나, Hb 농도의 유의한 변동은 관찰되지 않았다. 혈청 칼슘농도는 $50{\mu}g/L$ Cr의 농도에서 유의적인 감소를 보였고, 마그네슘농도는 $100{\mu}g/L$ Cr의 농도에서 유의적인 증가를 보였다. Glucose 및 total protein 농도는 가장 높은 크롬농도인 $200{\mu}g/L$ Cr에서 유의적인 증가를 보였으나, 중성지방인 triglyceride 농도는 노출기간 동안 유의한 변화가 관찰되지 않았다. GOT 및 GPT 활성은 $100{\mu}g/L$ Cr 이상의 농도에서 유의적인 증가를 보였고, ALP활성은 가장 높은 크롬농도인 $200{\mu}g/L$ Cr에서 유의적인 증가를 보였다. 이상의 결과는 숭어의 혈액성상과 혈청 무기성분은 크롬 농도 $50{\mu}g/L$ 이상, 혈청유기성분과 효소성분은 크롬농도 $100{\mu}g/L$이상에서 변동이 나타나는 것으로 결론 내릴 수 있다.

• 대한수의학회지
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• 제28권2호
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• pp.279-284
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• 1988

Observations were made on the blood picture of 50 multiparous and 98 infertile cows. The results obtained in this study were summarized as follows. 1. Mean values of RBC, Hb, PCV, MCV, MCH, MCHC and platelets for normal multiparous cows were $6.87{\pm}0.84{\times}10^6/mm^3$, $10.3{\pm}1.3g/100ml$, $31.4{\pm}3.7ml/100ml$, $46.1{\pm}6.9{\mu}^3$, $15.3{\pm}2.6pg$, $34{\pm}2.7%$, and $372.7{\pm}304.7{\times}10^3/mm^3$ respectively. 2. Mean values of RBC, Hb, PCV, MCV, MCH, MCHC and platelets for infertile cows were $7.00{\pm}0.98{\times}10^6/mm^3$, $10.6{\pm}1.2g/100ml$, $32.1{\pm}3.6ml/100ml$, $45.8{\pm}6.7{\mu}^3$, $15.3{\pm}1.8pg$, $33.9{\pm}3.3%$, and $382.7{\pm}157.5{\times}10^3/mm^3$, respectively. 3. Mean values of WBC count for normal multiparous and infertile cows were $7.92{\pm}1.72$ and $9.04{\pm}2.87{\times}10^3/mm^3$ respectively. 4. In the differential leukocytes count, mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for normal multiparous cows were $29.7{\pm}7.9%$, $56.8{\pm}7.6%$, $2.9{\pm}1.9%$, $9.9{\pm}5.3%$ and $0.3{\pm}0.7%$, respectively, and mean values of neutrophils, lymphocytes, monocytes, eosinophils and basophils for infertile cows were $31.9{\pm}7.8%$, $57.5{\pm}7.9%$, $2.6{\pm}1.9%$, $7.5{\pm}4.8%$, and $0.4{\pm}0.2%$, respectively.

• Journal of Trauma and Injury
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• 제25권4호
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• pp.139-144
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• 2012

Purpose: Hypovolemic shock is the leading cause of death in multiple trauma patients with pelvic bone fracures. The purpose of this study was to develop a simple prediction method for injury severity and amount of hemorrhage via an analysis of the correlation between the site of pelvic bone fracture and the amount of transfusion and to verify the usefulness of the such a simple scoring system. Methods: We analyzed retrospectively the medical records and radiologic examination of 102 patients who had been diagnosed as having a pelvic bone fracture and who had visited the Emergency Department between January 2007 and December 2011. Fracture sites in the pelvis were confirmed and re-classified anatomically as pubis, ilium or sacrum. A multiple linear regression analysis was performed on the amount of transfusion, and a simplified scoring system was developed. The predictive value of the amount of transfusion for the scoring system as verified by using the receiver operating characteristics (ROC). The area under the curve of the ROC was compared with the injury severity score (ISS). Results: From among the 102 patients, 97 patients (M:F=68:29, mean $age=46.7{\pm}16.6years$) were enrolled for analysis. The average ISS of the patients was $16.2{\pm}7.9$, and the average amount of packed RBC transfusion for 24 hr was $3.9{\pm}4.6units$. The regression equation resulting from the multiple linear regression analysis was 'packed RBC units=1.40${\times}$(sacrum fracture)+1.72${\times}$(pubis fracture)+1.67${\times}$(ilium fracture)+0.36' and was found to be suitable (p=0.005). We simplified the regression equation to 'Pelvic Bleeding Score=sacrum+pubis+ilium.' Each fractured site was scored as 0(no fracture) point, 1(right or left) point, or 2(both) points. Sacrum had only 0 or 1 point. The score ranged from 0 to 5. The area under the curve (AUC) of the ROC was 0.718 (95% CI: 0.588-0.848, p=0.009). For an upper Pelvis Bleeding Score of 3 points, the sensitivity of the prediction for a massive transfusion was 71.4%, and the specificity was 69.9%. Conclusion: We developed a simplified scoring system for the anatomical fracture sites in the pelvis to predict the requirement for a transfusion (Pelvis Bleeding Score (PBS)). The PBS, compared with the ISS, is considered a useful predictor of the need for a transfusion during initial management.

• 핵의학기술
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• 제12권1호
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• pp.33-38
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• 2008

Purpose: The Gated cardiac blood pool scan is non-invasive method that a quantitative evaluation of left ventricular function. Also this scan have shown the value of radionuclide ejection fraction measurements during the course of chemotherapy as a predictor of cardiac toxicity. Therefore a reliable method of monitoring its cardiotoxic effects is necessary. the purpose of this study is to minimize the overestimate of left ventricular ejection fraction (LVEF) by modified body position to reduce the influence of scattered rays from surrounding organs of the heart in the background region of interest. Materials and Methods: Gated cardiac blood pool scan using in vivo $^{99m}Tc$-red blood cell (RBC) was carried out in 20 patients (mean $44.8{\pm}8.6$ yr) with chemotherapy for a breast carcinoma. Data acquisition requires about 600 seconds and 24 frames of one heart cycle by the multigated acquisition mode, Synchronization deteriorates toward the end of the cycle and with the distance from the trigger signal (R-wave) by ECG gating. Gated cardiac blood pool scan was studied with conventional method (supine position and the detector head in $30-45^{\circ}$ left anterior oblique position and caudal $10-20^{\circ}$ tilt) and compared with modified method (left lateral flexion position with 360 mL of drinking water). LVEF analysis was performed by using the automatically computer mode. Results: The ROI counts of modified scan method were lower than LV conventional method ($1429{\pm}251$ versus $1853{\pm}243$, <0.01). And LVEF of modified method was also decrease compared with conventional method ($58.3{\pm}5.6%$ versus $65.3{\pm}6.1%$, <0.01). Imaging analysis indicated that stomach was expanded because of water and spleen position was changed to lateral inferior compared with conventional method. Conclusion: This study shows that the modified method in MUGA reduce the influence of scattered rays from surrounding organs. Because after change the body position to left lateral flexion and drinking water, the location of spleen, left lobe of liver and stomach had changed and they could escaped from background ROI. Therefore, modified method could help to minimize the overestimate LVEF (%).

• 대한수의사회지
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• 제20권4호
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• pp.233-238
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• 1984

본 시험은 천연기념물 제265호인 182일령 한국재래종 오골계 20수(남 ; 10, 여 ; 10수)를 대상으로 정상혈액상을 구명하고 성별로 비교하여 유의성을 검정했던 바 다음과 같은 성적을 얻었기에 보고하는 바이다. 1. 적혈구치, 혈색소치 및 적혈구용적은 각각평균 $3.23\pm0.29\times10^6/{\mu}{\iota}$, 11.75$\pm$2.27g/100m1 및 33.41$\pm$4.85ml/100m1를 나타냈으며 이들은 공히 수컷이 암컷에 비해 그 수치가 다소 높았으나 성별 간에 유의성은 없었다. 2. 평균적혈구용적, 평균적혈구혈색소양 및 평균적혈구혈색소농도의 평균치는 각각 102.65$\pm$17.45f1, 36.36$\pm$6.20pg, 및 34.44$\pm$0.63g/100m1을 나타냈으며 성별간에 비슷한 경향이었다. 3. 백혈구수는 평균 $16.33\pm4.75\times10^3/{\mu}{\iota}$ 이고 성별간의 유의성은 없었으며 동요범위는 매우 넓었다. 4. 혈소판수의 평균치는 $2.85\pm0.43\times10^5/{\mu}{\iota}$ 이고 남; 2.79$\pm$0.55, 여 $2.95\pm0.37\times10^5/{\mu}{\iota}$ 으로서 남이 여에 비하여 다소 높았으나 성별간에 유의차는 인정되지 않았다. 5. 백혈구감별계수의 평균치는 Neutrophile $25.8\pm5.32\%$, Lymphocyte $59.1\pm6.48\%$, Eosinophile $3.0\pm1.50\%$, Basophile $2.6\pm1.14\%$ 및 Monocyte $9.5\pm2.95\%$로 성별간에 비슷한 수치를 보였다. 6. 적혈구의 크기는 장경$\times$단경$\times$두께가 남 $13.06\pm0.53{\mu}{\times}8.66\pm0.22{\mu}{\times}3.49\pm0.07{\mu}$, 여 $12.71\pm0.51{\mu}{\times}8.55\pm0.24{\mu}{\times}3.44\pm0.06{\mu}$을 나타내었다. 7. 각 측정치에 있어서 성별간에 대체적으로 차이를 보였으나 유의차는 인정되지 않았다.

• 한국산학기술학회논문지
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• 제8권5호
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• pp.1266-1272
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• 2007

본 연구는 육성돈에 있어 복합생균제 급여가 생산성, 영양소 소화율, 혈액성상 및 분뇨내 악취물질 발생량에 미치는 효과를 규명하고자 실시하였다. 육성돈 48두를 공시하였으며, 시험 개시시 체중은 20.12kg이었다. 시험설계는 1) CON (basal diet), 2) Pro1 (basal diet + 0.1% complex probiotics) and 3) Pro2 (basal diet + 0.2% complex probiotics)으로 총 3처리를 하여 처리당 8반복, 반복당 2두씩 완전임의 배치하였다. 사양시험은 6주간 실시하였다 전체 시험기간동안 일당증체량은 생균제 첨가 수준이 증가함에 따라 유의적으로 증가하였다(P<0.05). 그러나 생균제 급여는 일당사료섭취량 및 사료효율에 영향을 미치지 못하였다. 복합 생균제 급여는 건물 소화율을 증가시켰다(linear effect, P<.0.05). 또한 질소 소화율을 개선시켰으며, Pro 1 처리구가 가장 높게 나타났다(linear and quadratic effect, P<0.05). 생균제 급여는 혈액내 WBC, RBC, lymphocyte 및 BUN함량에 영향을 미치지 못하였다. 생균제 첨가 수준의 증가함에 따라 분뇨내 $NH_3-N$ 발생량은 감소하였으며(linear and quadratic effect, P<0.05), $H_2S$ 발생량은 감소하였다(linear and quadratic effect, P<0.05). 결론적으로 사료내 복합생균제의 첨가 급여는 생산성을 증가시켰으며, 분뇨내 악취물질 발생량을 감소 시켰다.

• 한국가축번식학회지
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• 제23권3호
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• pp.247-256
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• 1999

본 연구는 한국야생 유래 행동이상 마우스(M. m. molossinus-tt@Kist)의 혈액학 및 혈액생화학치와, 체외수정란과 정자동결보존법, 체외수정과 수정란 이식기법을 이용한 번식장애 개선과 병원성 미생물이 제거된 무병마우스 생산을 위하여 실시하였다. 1. 5주령의 혈액학치에서 RBC, platelet치는 근교계에 비해 높게 나타났다. 혈액생화학치에서는 total cholesterol치가 근교계에 비해 높게 나타났으나 triglyceride, total protein, albumin치는 유사하였다. 2. 과배란 유기시의 평균 채란 수는 PMSG/hCG를 2.5/2.5 lU 투여군에서 11.6개, 5.0/5.0 IU 투여군에서 12.7개로 통계학적 유의 차는 인정되지 않았다. 3. 2.5/2.5 lU 투여군과 5.0/5.0 lU 투여군의 수정율은 각각 87.9%와 52%로 2.5/2.5 lU 부여군이 유의성있게 높은 성적을 나타내었고(p<0.05), 2세포기로의 발달율도 각각 유의성이 인정되는 99.0%와 90.6%였다 (p<0.05). 4. 동결정자를 이용한 체외수정에서의 수정율은 24.8%로 신선정자를 사용했을 때의 87.9% 보다 낮은 성적이었다. 5. 체외 수정란의 이식후의 산자율은 동결 2세포기 체외수정란의 경우 5마리(6.6%), 동결 정사를 이용한 체외 수정란의 경우 6마리(19.4%)와 대조군의 체외수정란의 경우 10마리(21.7%)의 새끼를 얻었다. 6. 이식후 출산한 산자의 미생물학적 검사에서 MHV(Mouse hepatitis virus)와 Staphylo-coccus aureus 등의 병원성 미생물이 무병대리모를 이용한 수정란이식에 의해 제거되었음을 확인하였다.

• 대한수의학회지
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• 제28권2호
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• pp.285-297
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• 1988

The present study was conducted in order to get the normal hematologic values of Korean Jindo dogs. Blood samples were taken from 328(♂ 92, ♂ 236) healthy Jindo dogs in Jindo area. The results obtained in this study were summarized as follows: 1. The mean values of red blood cell(RBC) count, hemoglobin(Hb) content and packed cell volume(PCV) in the age group of less than one year were 6.77(♂ 6.80, ♂ 6.76)${\times}10^6/{\mu}l$, 13.14 (♂ 13.53, ♂ 12.99)g/100ml and 43.28(♂ 44.47, ♂ 42.79)ml/100ml, respectively, whereas the RBC count, Hb content and PCV in the age group of one year and more were 7.42(♂ 7.44, ♂ 7.42)${\times}10^6/{\mu}l$, 14.98(♂ 15.56, ♂ 14.76)g/100ml and 47.18 (♂ 48.43, ♂ 46.71)ml/100ml, respectively. The values of RBC count, Hb content and PCV appeared to be higher in the male than in the female and it increased with aging in the age group of less than one year. 2. The mean values of mean corpuscular volume(MCV), mean corpuscular hemoglobin(MCH) and mean corpuscular hemoglobin content(MCHC) in the age group of less than one year were 63.93(♂ 65.40, ♂ 63.30)fl, 19.41(♂ 19.90, ♂ 19.22) pg and 30.36(♂ 30.43, ♂ 30.36)g/100ml respectively, whereas the MCV, MCH and MCHC in the age group of one year and more were 63.58(♂ 65.09, ♂ 62.95)fl, 20. 19(♂ 20.91, ♂ 19.89) pg and 31.75(♂ 32.13, ♂ 31.60)g/100ml respectively. The values of MCV, MCH and MCHC appeared to be higher in the male than in the female. No differences were found in the MCV and MCH between age groups. The MCHC appeared to be higher in older age group. 3. The mean values of white blood cell(WBC) count in the age group of less than one year were 14,356(♂ 13,878, ♂ 14,551)/${\mu}l$, whereas the values of WBC count in the age group of one year and more were 13,394(♂ 12,656, ♂ 13,672)/${\mu}l$. The WBC count appeared to be higher in the female than in the male. No differences were found between age groups in the WBC count. 4. In WBC differential count the mean percentage of lymphocyte, monocyte, neutrophil and eosinophil of the age group less than one year were 33.45(♂ 31. 64, ♂ 34.18), 2.77(♂ 3.00, ♂ 2.68), 57.40(♂ 58.18, ♂ 57.08) and 6.38(♂ 7.18, ♂ 6.06)% respectively, while those of the age group one year and more were 30. 22(♂ 28.38, ♂ 30.90), 3.03(♂ 3.23, ♂ 2.95), 60.93(♂ 63.34, ♂ 60.03) and 5.82(♂ 5.04, ♂ 6.11)% respectively. Lymphocyte count appeared to be higher in the female and in the age group below one year than in the male and in the age group of one year and more, whereas the reverse was the case with neutrophil and monocyte counts. 5. Mean platelet count in the age group of less than one year was 377,391(♂ 398,778, ♂ 368,721)/${\mu}l$, whereas mean platelet count in the age group of one year and more was 354,657 (♂ 373,660, ♂ 347,512)/${\mu}l$. The platelet count appeared to be higher in the male than in the female and especially lower in the age group of three years and more($305,513/{\mu}l$) than in the other age groups.

• 한국양식학회지
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• 제10권3호
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• pp.261-271
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• 1997

해수 순환여과식 양식 시스템의 여과 재료별 암모니아 제거 능력을 정량적으로 평가하기 위하여 5종류의 침지식 여과 재료인 모래(직경 2-3 mm), 산호사 (직경 30-50 mm), 자갈 (직경 20-40 mm), 폴리에틸렌 망 (네트론) 및 플라스틱 파판(스카이라이트)의 암모니아 제거량과 산소 소비량을 측정하고 그리고 회전원판식 여과 재료의 암모니아 제거량을 측정하여 비교하였다. 실험 기간중 사육조의 암모니아 농도는 0.052-0.904(평균 0.338$\pm$0.219) mg/l였고, 수온은 19.2-21.4 (평균 20.2$\pm0.58) ^{\circ}C$의 범위였으며 사육수의 암모니아 농도 (x : mg/l)에 대한 여과 재료별 단위 용적당 일간 암모니아 제거량 (y : g/m supper(3)/day)의 1/2-order kinetic 식과 1일 평균 암모니아 제거량(g/m supper(3)/day)은 다음과 같다. 모래 : Y=135.5X 상(0.5)-25.1 (r 상(2)=0.8110), 45.1 자갈 Y=43.7X 상(0.5)-5.5 (r 상(2)=0.2596), 17.1 산호사 : Y=125.1X 상(0.5)-33.0 (r 상(2)=0.7307), 31.8 폴리에틸렌 망 : Y=87.4X 상(0.5)-20.1 (r 상(2)=0.6780), 25.2 플라스틱 파판 : Y=71.1X 상(0.5)-17.6 (r 상(2)=0.5206), 19.2 회전원판 : Y=127.6X 상(0.5)-33.4 (r 상(2)=0.7146), 32.8 사육조의 용존산소 농도 범위 3.8-7.5 (평균 5.7$\pm0.98)\;mg/\ell$ 에서의 여과 재료 단위 용적($m^3$/day))당 1일 산소 소비량은 모래, 산호사, 폴리에틸렌망을 이용한 여과조에서 각각 442.1, 291.1, 및 236.9 g/$m^3$/day 로 모래 여과조의 산소 소비량이 가장 많았으며, 자갈과 플라스틱 파판은 각각 135.6 및 134.2 g/$m^3$/day로 가장 적었다. 한편 회전원판 여과조는 물에 노출되는 구조적 특성 때문에 산소 소비량 측정은 불가능하였다.