• 대한수의학회지
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• 제27권2호
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• pp.191-200
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• 1987

Attempts were to produce porcine leukocyte interferon(PorLeIF) and porcine immune interferon (PorIIF) in the culture of porcine leukocytes. The interferons produced were tested for antiviral activity against vesicular stomatitis virus on poreine-derived PK(15) cells, human-derived FL cells, and Korean native black goat-derived BGK cells. The results were summarized as follws: 1. In the isolation of porcine leukocytes, the mean isolation rate by the buffy coat separation method (28.7%) was higher than that by the hydroxyethyl starch-RBC sedimentation method (9.2%). 2. When NDV(BI)-induced PorLeIFs were assyed on PK(15) cells and FL cells, the mean titers were 129 IU/ml and 72 IU/ml respectively, being 55.8% of the activity in homologous species system expressed in heterologous system. 3. The activities of PHA P-induced PorIIFs were 197 IU/ml on PK (15) cells and no activity on human FL cells. The mean antiviral activity of PorIIF was 1.5 times that of PorLeIF in PK (15) cells. 4. The cytopathic effect of vesicular stomatitis virus was observed in BGK cells derived from Korean native black goat kidney permitting interferon assay on the cells. While the cross-species antiviral activity of reference human ${\alpha},\;{\beta}-interferon$ was observed on the cells, PorLeIF and PorIIF did not show any activity.

• Asian Pacific Journal of Cancer Prevention
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• 제13권6호
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• pp.2775-2780
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• 2012

In recent years there has been a substantial increase in the use of functional foods for disease control. Fruits and vegetables produce phytochemicals such as flavonoids and antioxidants which can lower oxidative stress and reduce the risk of chronic ailments like cancer. The aim of the present study was to investigate the antioxidant capacity and the possible protective effects of Amaranthus paniculatus leaves on the antioxidant defense system in Ehrlich's ascites carcinoma (EAC)-treated mice. Oral administration of the leaf extract at different doses caused a significant decrease in tumor volume, viable cell count and tumor weight and elevated the life span of EAC bearing mice. It also showed an improved antioxidant potential as evidenced by a significant increase in the cellular antioxidant defense system such as catalase, superoxide dismutase and reduced glutathione and also significantly reduced the levels of TBARS. The levels of RBC, hemoglobin and lymphocyte count were altered in EAC bearing mice and were reverted back to near normal levels after the treatment with the leaf extracts. Their adequate content of total phenolics and flavonoids, DPPH scavenging activity which further suggests that the extracts exert a significant protection against oxidative stress conditions.

• 한국미생물·생명공학회지
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• 제22권3호
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• pp.290-295
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• 1994

Micelle, liposome and polyethylene glycol(PEG) were employed to reduce the cell mem- brane toxicity of Amphotericin B(Amp. B). Cholesterol-sulfate which can form a mixed micelle with Amp. B molecules was found very effective for the reduction of Amp. B toxicity. 0.01% of cholesterol-sulfate could reduce the toxicity of 5X 10$^{-6}$ M Amp. B by 90%. The required concent- ration of cholesterol-sulfate for the toxicity reduction was proportionally increased with increasing Amp. B concentration. PEG was also effective on the reduction of Amp. B toxicity. 2% PEG was required for the reduction of toxicity by 50%, regardless of Amp. B concentration. The liposome system showed an effective reduction of Amp. B toxicity on RBC, maintaining the antibiotic effect on Candida albicans as free drugs. This seems to be due to the fact that liposome bilayer plays a role of buffer system between ergosterol of fungi cell membrane and cholesterol of red blood cell membrane, which leads the redistribution of Amp. B between them, as the result, the reduction of drug toxicity on cell membrane.

• 생명과학회지
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• 제19권6호
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• pp.825-831
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• 2009

본 연구에서는 저압.저산소환경의 노출을 시켜 30분간의 유산소성 자전거운동이 호흡기계 반응 및 면역기능의 응답에 미치는 영향을 검토하였다. 그 결과 몇 가지 지견을 도출해낼 수 있었다. 첫째로 75 $%HR_{max}$ 강도에서 30분간의 유산소성 운동 중에 저산소성 환기억제 또는 저하(hypoxic ventilatory depression or decline)라고 하는 현상은 평지뿐만이 아니라 3,000 m 고도에서도 나타나고 있고, 둘째 호흡기계의 반응은 축구선들이 특히 고소에서 적응과 감수성이 우수하다고 할 수 있으며, 셋째 면역기능의 항목인 단구(monocyte)는 운동습관의 유무 및 3,000 m의 고도에 따라 서로 다른 양상(상호작용)으로 변화를 하고 있다고 할 수 있다.

• 한국전자통신학회논문지
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• 제18권4호
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• pp.563-570
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• 2023

안전전송장치는 열차와 철도 시설 간에 통신을 제공하여 안전하고 효율적인 운영을 보장해야 하는 철도 시스템에서 사용되는 중요한 장치이다. 본 논문에서는 무선통신기반 차세대 열차제어시스템의 안전전송장치를 설계하고 시험하였다. 유럽규격과 국내규격을 분석하고 요구사항을 검토하여 국제적 수준의 안전전송장치의 HW 설계와 SW를 설계하여 제작하였다. 안전전송장치의 기능을 검증하기 위하여 시뮬레이터를 제작하여 설계된 기능이 구현되고 있는 것을 확인하였다.

• 한국동물위생학회지
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• 제33권1호
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• pp.89-96
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• 2010

Hematological and serum biochemical values were assessed from 20 clinically healthy Japanese Macaques raised in Everland Zoological Gardens and compared to the International Species Information System (ISIS) reference range that is used internationally as standard for wildlife animals. Taking our standard on sexual maturation at age 4, tRBC values in Macaques under age 4 were significantly lower than those over age 4, but the Hb and PCV values were significantly higher. Compared to the ISIS standard, the tRBC values in Macaques under age 4 were significantly lower whereas the Hb and MCHC values were significantly higher. Moreover, in the samples of Macaques over age 4, the PCV and MCV values were significantly lower than the ISIS standard. On serum biochemistry values the creatinine and amylase values in the Macaques under age 4 were significantly lower than those over age 4. In comparison with the ISIS standard, the values of ALT, ALP, BUN, IP, $Ca^{2+}$ and $K^+$ in the Macaques under age 4 did have no significant difference. The values of TP, GGT, tBil, amylase, TG and UA were significantly higher than the ISIS standard, but the values of albumin, AST, glucose, creatinine, cholesterol, CPK, LDH, $Na^+$ and Clwere significantly lower. In contrast, the values of TP, albumin, ALT, ALP, creatinine, cholesterol, amylase, TG, IP and $Na^+$ in the Macaques over age 4 did have no significant difference, but the values of GGT, BUN, tBil, UA and $Ca^{2+}$ were significantly higher, while the values of AST, glucose, CPK, LDH, $K^+$ and $Cl^-$ were significantly lower. On the other hand, there was no significant difference in hematological and serum biochemical values between the groups of male and female.

• 한국산학기술학회논문지
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• 제8권4호
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• pp.861-866
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• 2007

본 연구는 하 폐수 중의 유기물뿐만 아니라 질소, 인을 생물학적으로 제거하기 위하여 Bacillus sp. 미생물을 사용하였다. Bacillus sp. 미생물이 생존하기에 알맞지 않은 상황에서는 포자를 형성하고, 증식과 포자화를 반복하면서 우점화되는 특성을 발현시키기 위해 점감포기를 실시하였다. 반응기 용존산소 농도는 포기조 1단; $1.2{\sim}l.5mg/L$, 포기조 2단; $0.3{\sim}$0.5mg/L, 포기조 3단 ; 0.2mg/L이하로 유지하였다. 또한 공정내 미생물의 유실을 방지하고 미생물이 고농도 상태로 유지 가능한 부착성장의 한 공법인 RBC에 끈상 나선형 미생물 접촉재를 설치한 반응기를 이용하였다. 식종한 Bacillus sp.가 적응하는 기간에서의 유입유량은 173 L/d, 내부반송율 200%, 슬러지반송율 100%로 운전을 하였으며, 방류수질을 기준으로 단계적으로 유입유량을 증가시켰다. 정상상태에서 유입유량은 346 L/d이고, 내부반송율과 슬러지 반송율은 각각 50%로 결정하여 실험을 수행하였고, 원수는 Glucose 1,800 mg/L. $NH_4Cl\;500mg/L,\;KH_2PO_4\;5mg/L$를 혼합한 인공폐수를 제조하여 공정에 주입하였고, 그 결과 각각 미생물이 폐수에 적응하는 단계인 Period 1에서는 각 수질 분석 항목의 농도가 점차적으로 감소하는 경향을 보였으며. 정상상태라고 판단한 Period 2에서는 최종적으로 유입수에 대한 유출수의 제거율은 각각 TCODCr 94%. BOD 87%, T-N 85%, T-P 89%의 결과를 나타내었다.

• The Korean Journal of Pain
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• 제34권4호
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• pp.427-436
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• 2021

Background: Pharmacological and non-pharmacological therapies have been used to treat patients with chemotherapy-induced peripheral neuropathy (CIPN). However, the effect of therapies in cancer patients has yet to be investigated comprehensively. We hypothesized that cyclic thermal therapy would improve blood flow and microcirculation and improve the symptoms driven by CIPN. Methods: The criteria of assessment were blood volume in region of interest (ROI) in the images, and European Organization for Research and Treatment of Cancer-Quality of Life Questionnaire-Chemotherapy-Induced Peripheral Neuropathy 20 questionnaire scores. The blood volume was quantified by using red blood cell (RBC) scintigraphy. All patients were treated 10 times during 10 days. The thermal stimulations, between 15° and 41°, were repeatedly delivered to the patient's hands. Results: The total score of the questionnaires, the score of questions related to the upper limbs, the score of questions closely related to the upper limbs, and the score excluding the upper limbs questions was decreased. The blood volume was decreased, and the variance of blood volume was decreased. During cooling stimulation, the blood volume was decreased, and its variance was decreased. During warming stimulation, the blood volume was decreased, and its variance was decreased. Conclusions: We suggest that cyclic thermal therapy is useful to alleviate CIPN symptoms by blood circulation improvement. RBC scintigraphy can provide the quantitative information on blood volume under certain conditions such as stress, as well as rest, in peripheral tissue.

• Applied Science and Convergence Technology
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• 제24권5호
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• pp.196-202
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• 2015

Human blood consists of 55% of plasma and 45% of blood cells such as white blood cell (WBC) and red blood cell (RBC). In plasma, there are many kinds of promising biomarkers, which can be used for the diagnosis of various diseases and biological analysis. For diagnostic tools such as a lab-on-a-chip (LOC), blood plasma separation is a fundamental step for accomplishing a high performance in the detection of a disease. Highly efficient separators can increase the sensitivity and selectivity of biosensors and reduce diagnostic time. In order to achieve a higher yield in blood plasma separation, we propose a novel fluid wing structure that is optimized by COMSOL simulations by varying the fluidic channel width and the angle of the bifurcation. The fluid wing structure is inspired by the inertial particle separator system in helicopters where sand particles are prevented from following the air flow to an engine. The structure is ameliorated in order to satisfy biological and fluidic requirements at the micro scale to achieve high plasma yield and separation efficiency. In this study, we fabricated the fluid wing structure for the efficient microfluidic blood plasma separation. The high plasma yield of 67% is achieved with a channel width of $20{\mu}m$ in the fabricated fluidic chip and the result was not affected by the angle of the bifurcation.

• 대한지역사회영양학회지
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• 제4권2호
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• pp.149-156
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• 1999

This study was due to investigate the dietary intakes, serum lipids and other hematological indices in female adolescent smokers. The subjects were 85 smokers, whose average pack-year(smoking years on the basis of one pack of cigarettes per day) was 1.26 and 87 nonsmokers who were female high school students in Seoul. An anthropometric measreument was performed and % body fat was also analyzed by the Bioelectrical Impedance Fatness Analyzer(GIF-891). Dietary intakes and food habits were examined through questionnaires and nutrient intakes were analyzed by the Computer Aided. Nutritional analysis program for professional(CAN-pro). Serum TG, HDL-cholesterol and total-cholesterol levels were measured with test kits. Serum glucose, albumin, GOT and GPT were measured by automated dry chemistry system, SPOTCHEM 4410. Hemoglobin, hematocrit, RBC, WBC and MCV were determined by Semi Automated Microcell Counter(F-520). All data were statistically analyzed by SAS PC package program. There was no significant difference in the anthropometric measurements between smokers and nonsmokers. The caloric intake in adolescent smokers tended to be higher than that of nonsmokers but the difference was not statistically significant. In addition, there was no significant difference between smokers and nonsmokers in biochemical indices. Analysis of serum lipids showed that the serum levels of total-cholesterol and LDL-cholesterol of nonsmokers were unexpectedly significantly higher(p<0.05) than those of smokers. Overall results indicate that smoking itself with short pack-year in healthy female adolescent did not seem to influence apparent health and nutritional status.