• 환경생물
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• 제37권1호
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• pp.88-92
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• 2019

본 연구에서는 한국산 대륙유혈목이 Hebius vibakari의 집단 번식 사례를 보고하고자 한다. 2009년 5월 20일 전라남도 진도군 맹골도리에 위치한 맹골도에서 대륙유혈목이의 집단 번식이 관찰되었다. 맹골도 섬 능선 위에 형성된 바위지대(고도 107 m)에서 번식이 관찰되었다. 대륙유혈목이 암컷 6개체와 수컷 5개체는 번식 행동 중 하나인 교미공(mating ball)을 형성하고 있었다. 그 중 2 쌍은 뱀류에서 관찰되는 짝짓기 행동인 "꼬리 뒤엉킴(tail wrestling)" 중이었다. 집단 번식이 관찰된 당시의 기온과 습도는 각각 $25.7^{\circ}C$와 59%였다. 본 연구의 결과는 희귀 파충류인 대륙유혈목이의 집단 번식을 국내 최초로 보고한 사례이다.

• 식물분류학회지
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• 제49권3호
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• pp.240-252
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• 2019

20세기초 한반도에서 채집된 고표본들은 한국전쟁 동안 대부분이 소실되었으나, 일본 도쿄대학교 표본관(TI)에 소장된 한반도 고표본들은 온전하게 보존되어 있다. 북한 식물에 대한 정보를 얻을 수 없는 현 상황에서 TI의 한국산 고표본 정보는 매우 중요하다. TI 수장고에서 최근 확인된 미동정 상태의 한반도 고표본을 동정하여 목록을 작성했으며, 중복채집품(duplicate)을 확보했다. 곤충학자인 Ikuma Yoichiro는 1913년 8월 1일부터 23일까지 청진에서 백두산을 거쳐 혜산진에 이르는 일정 동안 60과 177속 240종류의 식물을 채집했다.

• Journal of the Korean Wood Science and Technology
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• 제49권6호
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• pp.591-601
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• 2021

Recently it is trend to increase wood use as carbon neutral materials, there is recognized to need necessarily durability improvement of wooden building. It is very rare to report existing on the identification of isolates causing discoloration in domestic weathered wooden building used for long period. The objective of this study was identification of fungi that cause discoloration on the exteriors of weathered domestic wooden buildings in the southern part of South Korea. Our findings can be helpful to establish protection technology for weather deterioration of domestic wooden buildings. Wood chip samples presumed to be contaminated with sapstain fungi were collected from the surface of wooden members used in wooden buildings at Jangheung, Jeollanam-do (two locations, #13 and #14), and Jeju Island (two locations, #31 and #33). The growth of microorganisms was confirmed by performing culture tests for the collected samples, and fungi were isolated, purified, and identified. The results indicated that the fungal strains isolated from wooden buildings #13 and #14 at Jangheung, Jeollanam-do, were 99.83% and 100% homologous to Aureobasidium melanogenum, respectively. For wooden building #31 at Jeju Island (two locations), the fungal strain isolated was 100% homologous to A. melanogenum, which is the same species isolated from the wooden buildings at Jangheung. The fungal strain isolated from wooden building #33 (Jeju Island) had 99.83% homology with A. pullulans, which is commonly found in wood degraded by weather or ultraviolet rays. Our findings can be utilized as a basis for establishing protection technology in domestic wooden buildings.

• Pediatric Infection and Vaccine
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• 제29권1호
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• pp.54-60
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• 2022

살모넬라 수막염은 소아청소년에서 흔하지 않은 병이나 심각한 신경학적 합병증을 일으킬 수 있다. 영아, 특히 3개월 미만의 연령과 악성 종양, 말라리아 감염, 인체면역결핍바이러스 감염과 같은 면역 저하 상태는 살모넬라 수막염의 위험인자로 알려져 있다. 본 증례에서는 고열과 구토, 의식 변화를 주소로 내원한 이전 특이병력 없던 건강한 8세 여아의 살모넬라 수막염 증례를 소개한다. 환자의 뇌척수액에서 D군 살모넬라가 배양되었으며, 뇌 자기공명영상 소견은 정상이었다. 면역 글로불린 수치와 림프구 수는 정상 범위였고, 차세대 염기서열 분석에서 원발성 면역결핍 질환을 일으키는 유전자 변이는 검출되지 않았다. 3세대 세팔로스포린 투약으로 환자의 증상은 빠르게 호전되었으며 합병증이나 후유증은 발생하지 않았다. 비장티푸스 살모넬라균은 면역이 정상인 소아에서 수막염을 일으킬 수 있으며 조기에 항생제를 투여하면 성공적으로 치료할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제32권7호
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• pp.892-901
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• 2022

The rising demand for carotenoids can be met by microbial biosynthesis as a promising alternative to chemical synthesis and plant extraction. Several species of lactic acid bacteria (LAB) specifically produce C₃₀ carotenoids and offer the added probiotic benefit of improved gut health and protection against chronic conditions. In this study, the recently characterized Lactiplantibacillus plantarum subsp. plantarum KCCP11226^T produced the rare C₃₀ carotenoid, 4,4'-diaponeurosporene, and its yield was optimized for industrial production. The one-factor-at-a-time (OFAT) method was used to screen carbon and nitrogen sources, while the abiotic stresses of temperature, pH, and salinity, were evaluated for their effects on 4,4'-diaponeurosporene production. Lactose and beef extract were ideal for optimal carotenoid production at 25℃ incubation in pH 7.0 medium with no salt. The main factors influencing 4,4'-diaponeurosporene yields, namely lactose level, beef extract concentration and initial pH, were enhanced using the Box-Behnken design under response surface methodology (RSM). Compared to commercial MRS medium, there was a 3.3-fold increase in carotenoid production in the optimized conditions of 15% lactose, 8.3% beef extract and initial pH of 6.9, producing a 4,4'-diaponeurosporene concentration of 0.033 A₄₇₀/ml. To substantiate upscaling for industrial application, the optimal aeration rate in a 5 L fermentor was 0.3 vvm. This resulted in a further 3.8-fold increase in 4,4'-diaponeurosporene production, with a concentration of 0.042 A₄₇₀/ml, compared to the flask-scale cultivation in commercial MRS medium. The present work confirms the optimization and scale-up feasibility of enhanced 4,4'-diaponeurosporene production by L. plantarum subsp. plantarum KCCP11226^T.

• Journal of Veterinary Science
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• 제21권1호
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• pp.9.1-9.15
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• 2020

Regenerative therapy holds great promise in the development of cures of some untreatable diseases such as cardiovascular diseases, and pluripotent stem cells (PSCs) including induced PSCs (iPSCs) are the most important regenerative seed cells. Recently, differentiation of human PSCs into functional tissues and cells in vitro has been widely reported. However, although porcine reports are rare they are quite essential, as the pig is an important animal model for the in vitro generation of human organs. In this study, we reprogramed porcine embryonic fibroblasts into porcine iPSCs (piPSCs), and differentiated them into cluster of differentiation 31 (CD31)-positive endothelial cells (ECs) (piPSC-derived ECs, piPS-ECs) using an optimized single-layer culture method. During differentiation, we observed that a combination of GSK3β inhibitor (CHIR99021) and bone morphogenetic protein 4 (BMP4) promoted mesodermal differentiation, resulting in higher proportions of CD31-positive cells than those from separate CHIR99021 or BMP4 treatment. Importantly, the piPS-ECs showed comparable morphological and functional properties to immortalized porcine aortic ECs, which are capable of taking up low-density lipoprotein and forming network structures on Matrigel. Our study, which is the first trial on a species other than human and mouse, has provided an optimized single-layer culture method for obtaining ECs from porcine PSCs. Our approach can be beneficial when evaluating autologous EC transplantation in pig models.

• Journal of Forest and Environmental Science
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• 제26권3호
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• pp.219-223
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• 2010

It has long been recognized that wild vegetation are sort of improving fitness. Many peoples are now ingesting them instead of the vegetables that are grown in green house. In this regard, Parasenecio firmus, which is considered to be one of the rare species in Korea, has been given a lot of attentions as edible green plants recently. Hence it may be necessary to provide the information people who want to commercially cultivate the plants. Regarding appropriate forest stands that are fit for the cultivation. Firstly, we collected the seeds from 4 natural habitats to find out how many seeds are fully matured in the natural conditions. The quality of the seeds collected were found to be very low. However, it appeared as clear differences when screened seeds were grown in covered seed beds over winter. In this case the germination rate of the seeds was 95%, indicating that there may be no difficulty for mass production of plantlets. Secondly, in the field of planting test in an appropriate cultivating site among 6 of forest stands, those in Pinus koraiensis stand were the highest in survival rate, but somewhat low with those in Quercus mongolica and broad-leaved stands. Thus, it was demonstrated that forest stands dominated with conifer like P. koraiensis are more appropriate for the cultivation of P. firums.

• Advances in nano research
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• 제13권4호
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• pp.325-339
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• 2022

Endophytes ascertain a symbiotic relationship with plants as promoters of growth, defense mechanism etc. This study is a first report to screen the endophytic population in Waltheria indica, a tropical medicinal plant. 5 bacterial and 3 fungal strains in leaves, 3 bacterial and 1 yeast species in stems were differentiated morphologically and identified by biochemical and molecular methods. The phylogenetic tree of the isolated endophytes was constructed using MEGA X. Silver nanoparticles were biosynthesized from a rare endophytic bacterium Cupriavidus metallidurans isolated from the leaf of W. indica. The formation of silver nanoparticles was confirmed by UV-Visible spectrophotometer that evidenced a strong absorption band at 408.5 nm of UV-Visible range with crystalline nature and average particle size of 16.4 nm by Particle size analyzer. The Fourier Transform Infra-Red spectrum displayed the presence of various functional groups that stabilized the nanoparticles. X-ray diffraction peaks were conferred to face centered cubic structure. Transmission Electron Microscope and Scanning Electron Microscope revealed the spherical-shaped, polycrystalline nature with the presence of elemental silver analyzed by Energy Dispersive of X-Ray spectrum. Selected area electron diffraction also confirmed the orientation of AgNPs at 111, 200, 220, 311 planes similar to X-ray diffraction analysis. The synthesized nanoparticles are evaluated for antimicrobial activity against 7 bacterial and 3 fungal pathogens. A good zone of inhibition was observed against pathogenic bacteria than fungal pathogens. Thus the study could hold a key aspect in drug discovery research and other pharmacological conducts of human clinical conditions.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2020년도 춘계학술대회
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• pp.20-20
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• 2020

Eranthis stellata Maxim. is a perennial plant that grows around the valley. E. stellata is concerned about the decline in natural habitats due to climate change in KOREA, continues to be observed and protected as an endangered species (Least Concrned, LC). Nevertheless, studies on the characteristics of the seeds of E. stellata are insufficient. So, this study analyzed the morphological characteristics and dormancy types of seeds. Seeds of E. stellata was collected in April at Gyeongsangbuk-do Arboretum and kept at 5 ℃ until using. To investigate the morphology of seeds, an optical microscope and a scanning electron microscope (SEM) were used. GA3 treated or untreated seeds (4 replicates of 25 seeds each) were observed germination and embryo growth for 1 month at 5 ℃ and 25/15 ℃ (12h day/12h night). The seed surface of E. stellata, light brown, was observed as a common characteristic of Eranthis genus, reticulate. The short axis of seeds was 1.11~1.77mm (average 1.44mm), and the long axis was 1.27~1.91mm (average 1.63mm), which was investigated in a slightly round shape (subglose). While no germination was observed at all conditions, Embryo growth was observed at 5 ℃ both in the control group and with GA3treated groups. Thus, seeds of E. stellata are classified as morphological physiological dormancy (MDP), which requires embryonic development and dormant break at the same time. These results can be useful information for determining morphological physiological seed dormancy and germination, and will be an important basic data for seed propagation of E. stellata as a resource.

• 식물분류학회지
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• 제37권2호
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• pp.155-196
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• 2007

본 연구는 지리산 국립공원 지역에 대한 종합적인 식물상 연구로서 기존에 보고된 문헌들에 대한 고찰과 1년간의 실제적인 조사에 의해서 얻어진 표본자료를 근거로 하여 식물목록을 작성하였다. 실제 조사에서 밝혀진 지리산 국립공원지역의 관속식물 수는 실제 조사에서 확인된 분류군은 37목, 109과, 382속의 708분류군의 590종, 3아종, 95변종 및 20품종으로 이루어져 있었고, 22개의 문헌에만 나타나고 조사에서 확인되지 않은, 1,117분류군은 41목 130과 500속에 해당하는 901종 9아종 140변종 67품종 등으로 구성되어 있었다. 결론적으로 문헌 조사와 실제 야외 조사에서 밝혀진 지리산 소산식물의 총수는 1,825(708+1,117) 분류군으로 밝혀졌다. 이를 목록으로 제시하였다. 이 중에서 기존의 조사에서는 발견되지 않았으나 본 조사에서 최초로 밝혀진 분류군은 43분류군이었다. 실제 조사에서 확인된 식물들 중에서 한국특산 식물 31분류군, 산림청 지정 희귀 및 멸종위기식물 21분류군, 환경부 지정 식물구계학적 특정 식물종 중에서 3등급 이상에 해당하는 40분류군 등이 생육하고 있었으며, 27분류군의 한국 귀화식물이 밝혀졌다. 확인되지 않고 문헌조사에만 나타난 분류군 중에서 한국특산식물 45분류군, 산림청 지정 희귀 및 멸종위기식물 45분류군, 환경부 지정 식물구계학적 특정식물종 중에서 3등급 이상에 해당하는 121분류군 등이 생육하고 있으며 31분류군의 한국 귀화식물이 밝혀졌다. 이로 미루어 볼 때 지리산의 식생은 여러 가지 요인에 의해 서서히 변화되어 가고 있으며, 많은 중요한 식물 자원들이 사라져 가는 추세라고 판단되므로 이에 대한 대책 수립이 요구되어 진다.