• Journal of Plant Biotechnology
• /
• 제40권1호
• /
• pp.43-48
• /
• 2013

Oreorchis coreana Finet is threatened globally by over-collection from its natural habitats for horticultural purposes. Its rarity in nature makes this plant one of the most endangered species in Korea. In this study, we investigated the effects of sodium hypochlorite (NaOCl) on orchid seed viability and seed germination. An in vitro bioassay swelling test using immature seeds was compared with a standard chemical procedure using triphenyl tetrazolium chloride (TTC) to test seed viability. In general, the bioassay was more appropriate for estimating embryo viability after a prolonged pre-treatment (more than 1 h) in 1% NaOCl, a surface sterilant often used to enhance germination of seeds of terrestrial plants. Therefore, an efficient method for investigating in vitro swelling of immature seeds is urgently needed. We established a method for determining the viability and swelling of O. coreana seeds via in vitro examination of immature seeds. Treatment of immature seeds with 1% NaOCl for 10 min greatly enhanced the extent of swelling of immature zygote embryos when compared to untreated seeds. These data obtained here appear to be comparable to viability and swelling that occurs in O. coreana seeds via asymbiotic germination.

• 한국수의병리학회:학술대회논문집
• /
• 한국수의병리학회 2003년도 추계학술대회초록집
• /
• pp.51-51
• /
• 2003

Hepatocellular carcinoma (HCC) is major type of cancer causing deaths on worldwide and has been reported in numerous species, including dogs, cats, sheep, pigs, fowl, woodchucks, trout and cows. The precise incidence data or complete age distribution of cattle is not known because this information was derived from abattoir. Moreover, there are very few reports in bovine HCC [l]. This report presents findings of bovine HCC with metastasis to the spleen, a very rare tumor in ruminant oncology, found in a Holstein cow during routine inspection at the abattoir. (omitted)

• Journal of Forest and Environmental Science
• /
• 제29권4호
• /
• pp.275-281
• /
• 2013

To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 ${\mu}M $induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 ${\mu}M $ thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 ${\mu}M $ treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 ${\mu}M $ showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (<10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.

• The Korean Journal of Ecology
• /
• 제23권5호
• /
• pp.391-395
• /
• 2000

본 조사는 1997년 4월부터 1998년 12월까지 경상남도 통영시 한산면의 한산도와 추봉도를 중심으로 이루어졌으며 식생분석을 위해 군락조성표를 작성하였다. 본 조사지역의 식생은 곰솔군락(전형하위군락, 굴참나무하위군락, 소사나무하위군락), 소나무군락, 후박나무군락, 편백식재림, 삼나무식재림, 억새군락 등 총 6개 군락과 3개 하위군락으로 구분되었다. 본 조사지역인 한산도 제승당 주변의 소나무군락은 식생이 가장 잘 보존되어 있는 지역이지만 도서 전체에 대한 광범위한 생태학적 측면에서의 복원이 필요하며 고란초와 같은 희귀식물, 후박나무와 곰솔 등의 노거수, 후박나무, 동백나무 분포지 등은 보호되어야 한다.

• 한국약용작물학회지
• /
• 제21권3호
• /
• pp.220-228
• /
• 2013

This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.

• 한국환경복원기술학회지
• /
• 제12권6호
• /
• pp.18-35
• /
• 2009

Distribution of vascular plants in Yangyang-gun were investigated 12 times from Apr. to Sept., 2008. In the result of survey, the flora of this area consist of 412 taxa totally; 89 families, 259 genera, 366 species, 5 subspecies, 37 varieties and 4 forms. There were remarkable plants such as 11 taxa of Korean endemic plants, 58 taxa of specific plants which were designated by the Ministry of Environment, 2 taxa of endangered plants of Ministry of Environment, 7 taxa of rare, endangered plants of Korea Forest Service and 28 taxa of afforestation plants and 296 taxa of useful plants. Furthermore, 36 taxa of naturalized plants (8.74%) were also observed in this area. Vegetation of investigated area was conserved well. However, vegetation has been destroyed rapidly due to construction of commercial complex for human recreation such as condominiums and stores. Therefore, it is need to efficient management of the water quality and vegetation by conservation strategies from huge visitors.

• 미생물학회지
• /
• 제35권1호
• /
• pp.61-64
• /
• 1999

토양으로부터 유기인계 살충제인 phosphamidon을 분해하는 세균들을 분리하고 Biolog system을 이용하여 동정하였다. 그람양성 세균들은 모두 Bacillus 속에 속하는 종들이었으며 그람음성 세균들은 토양에서 우점하지 않는 세균들이 많았다. 이들중 phosphamidon 함유배지에서 생장률이 높은 균주들을 선택하여 phophamidon 분해능을 조사한 결과 Capnocytophaga gingivalis 로 동정된 YD-17 균주가 가장 높은 생분해능을 나타내어 1000ppm의 phosphamidon 이 배양 21일 후 94%의 잔류량을 보였으며 이는 대조구에 비해 제거율이 52% 증가된 결과였다. 이 때 균주의 생장을 단백질량으로 측정하였는데 분해균주들이 고농도의 phosphamidon에 의해 저해되지 않고 지속적인 생장을 하여 phosphamidon을 탄소원으로 이용하는 생분해가 일어난 것을 확인할 수 있었다.

• 한국환경복원기술학회지
• /
• 제21권4호
• /
• pp.25-42
• /
• 2018

The purpose of this study is to present the basic data for management of ecologically restored stream by surveying and analysing the vascular plants distributed in Chunghyocheon stream, Gyeongju-si. The survey of vascular plants in this site was conducted before and after restoration. The number of vascular plants were summarized as 276 taxa including 68 families, 188 genera, 242 species, 1 subspecies, 29 varieties and 4 forms. The rare plants were 2 taxa including Prunus yedoensis Matsum. and Iris ensata var. spontanea (Makino) Nakai, and the Korean endemic plant was Lespedeza maritima Nakai. The specific plants by floristic region were 13 taxa including 1 taxa of grade V, 3 taxa of grade III, 1 taxa of grade II and 8 taxa of grade I. The naturalized plants were 62 taxa including Persicaria orientalis (L.) Spach, Lepidium virginicum L., Poa compressa L. and so forth. The invasive alien plants were 3 taxa including Ambrosia artemisiifolia L., Aster pilosus Willd. and Lactuca scariola L.. The hydrophytes were 34 taxa including 26 taxa of emergent plant, 1 taxa of floating-leaved plant, 2 taxa of free-floating plant and 5 taxa of submerged plant.

• Tuberculosis and Respiratory Diseases
• /
• 제46권4호
• /
• pp.574-579
• /
• 1999

저자들은 신증후군에 동반된 거대세포바이러스 폐렴 1예를 경험하였기에 문헌 고찰과 함께 보고하는 바이다.

• 식물분류학회지
• /
• 제38권4호
• /
• pp.565-571
• /
• 2008

미발굴 한반도 고유 식물자원의 발굴 관리를 위한 연구 조사 과정 중 국내 분포가 명확하지 않거나 극히 한정되어 있는 4종 식물의 신분포지가 확인되었다. 제주도 인근에서 자라는 난대 양치식물인 새깃아재비와 부생난인 으름난초가 전남 함평군 감방산과 전남 보성군 까치봉에서 각각 발견되었다. 전남지방에 분포하는 것으로 알려진 입술망초가 광주시 무등산에서 확인되었으며, 제주도와 울릉도에 자라는 분단나무가 강원도 강릉시 자병산에서 확인되었다.