Isolation, Identification , and Biodegradability of Phosphamidon-Degrading Bacteria

Phosphamidon 분해세균의 분리동정 및 생분해능

  • Published : 1999.03.01

Abstract

Organophosphorus inseclicide phosphamidon-degrading bacteria were isolated from agricultural soils and identified using Biolog microtiter assay. All Gram-positive degrading bacterial strains belong to genus Bacillus and many Gram-negative bacteria were rare soil species. Among them fast growing strains on phosphamidon-containing minimal medium were sclected and their biodegrading capability wcre measured. YD-17 which was identified as Capnocytophaga gingivalis showed the highest biodegradation rate. It could incrcase the removal of phosphamidon up to 52%. During the biodegradation continuous increase of amount of cell protein was observed, which indicated that phosphamidon was utilized as a carbon source for phosphamidon-degrading bacteria.

토양으로부터 유기인계 살충제인 phosphamidon을 분해하는 세균들을 분리하고 Biolog system을 이용하여 동정하였다. 그람양성 세균들은 모두 Bacillus 속에 속하는 종들이었으며 그람음성 세균들은 토양에서 우점하지 않는 세균들이 많았다. 이들중 phosphamidon 함유배지에서 생장률이 높은 균주들을 선택하여 phophamidon 분해능을 조사한 결과 Capnocytophaga gingivalis 로 동정된 YD-17 균주가 가장 높은 생분해능을 나타내어 1000ppm의 phosphamidon 이 배양 21일 후 94%의 잔류량을 보였으며 이는 대조구에 비해 제거율이 52% 증가된 결과였다. 이 때 균주의 생장을 단백질량으로 측정하였는데 분해균주들이 고농도의 phosphamidon에 의해 저해되지 않고 지속적인 생장을 하여 phosphamidon을 탄소원으로 이용하는 생분해가 일어난 것을 확인할 수 있었다.

Keywords

References

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