• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.43-48
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• 2013

Oreorchis coreana Finet is threatened globally by over-collection from its natural habitats for horticultural purposes. Its rarity in nature makes this plant one of the most endangered species in Korea. In this study, we investigated the effects of sodium hypochlorite (NaOCl) on orchid seed viability and seed germination. An in vitro bioassay swelling test using immature seeds was compared with a standard chemical procedure using triphenyl tetrazolium chloride (TTC) to test seed viability. In general, the bioassay was more appropriate for estimating embryo viability after a prolonged pre-treatment (more than 1 h) in 1% NaOCl, a surface sterilant often used to enhance germination of seeds of terrestrial plants. Therefore, an efficient method for investigating in vitro swelling of immature seeds is urgently needed. We established a method for determining the viability and swelling of O. coreana seeds via in vitro examination of immature seeds. Treatment of immature seeds with 1% NaOCl for 10 min greatly enhanced the extent of swelling of immature zygote embryos when compared to untreated seeds. These data obtained here appear to be comparable to viability and swelling that occurs in O. coreana seeds via asymbiotic germination.

• Proceedings of the Korean Society of Veterinary Pathology Conference
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• 2003.10a
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• pp.51-51
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• 2003

Hepatocellular carcinoma (HCC) is major type of cancer causing deaths on worldwide and has been reported in numerous species, including dogs, cats, sheep, pigs, fowl, woodchucks, trout and cows. The precise incidence data or complete age distribution of cattle is not known because this information was derived from abattoir. Moreover, there are very few reports in bovine HCC [l]. This report presents findings of bovine HCC with metastasis to the spleen, a very rare tumor in ruminant oncology, found in a Holstein cow during routine inspection at the abattoir. (omitted)

• Journal of Forest and Environmental Science
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• v.29 no.4
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• pp.275-281
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• 2013

To establish in vitro nodal culture conditions of Echinosophora koreensis Nakai, one of rare and endangered species famous for beautiful flowers in the Korean Peninsula, the influence of plant growth regulators (PGRs) on shooting and rooting from in vitro shoots was investigated. In shoot multiplication, addition of 6-benzylaminopurine (BA) to the half-strength Driver and Kuniyuki's media in the range of 2.22 to 8.88 ${\mu}M $induced 2.5 to 2.7 shoots per axillary bud; and addition of 2.27 ${\mu}M $ thidiazuron (TDZ) produced 3.2 shoots, during 4 weeks of culture, while zeatin and isopentenyl adenine (2ip) were not effective on shoot multiplication as observed from several combination treatments of BA with other PGRs. Shoots established were smaller than 2 cm in length, in most of the treatments. while in BA 8.88 ${\mu}M $ treatment more than 30% of shoots were longer than 2 cm and shorter than 4 cm. In rooting, naphthalene acetic acid (NAA) from 5.37 to 21.48 ${\mu}M $ showed the rooting rate from 40.0 to 62.5%. Indole butyric acid (IBA) addition had little effect on rooting (<10%), although some roots in IBA-containing media were longer than those in NAA. Micropropagation from axillary buds of nodular explants was applicable and promising to multiplication and conservation of Echinosophora koreensis Nakai.

• The Korean Journal of Ecology
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• v.23 no.5
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• pp.391-395
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• 2000

The vegetations of Hansan and Chubong islets in Tongyoungsi were investigated from April, 1997 to December, 1998 and a synthesis table was constructed. Based on the synthesis table, the vegetation was classfied into 6 communties: Pinus thunbergii community, Pinus densiflora community, Machilus thunbergii community, Chamaecyparis obtusa afforestation, Cryptomeria japonica afforestation, Miscanthus sinensis var. purpurascens community, and 3 subcommunities: Pinus thunbergii typical subcommunity, Quercus variabilis subcommunity, Carpinus coreana subcommunity. The vegetation of Pinus densiflora community around Jeseungdang area is well preserved. However the islet requirs the ecological restoration, and we suggest that the rare species such a Crypsinus hastatus, old trees of Machilus thunbergii and Pinus thunbergii, and the habitat for Machilus thunbergii and Camellia japonica must be preserved.

• Korean Journal of Medicinal Crop Science
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• v.21 no.3
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• pp.220-228
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• 2013

This study was conducted to assess the genetic diversity and population structure of 70 amaranth accessions collected from South and Southeast Asia using 14 simple sequence repeat (SSR) markers. In total, 67 alleles were detected, with an average of 4.79 per locus. Rare alleles comprised a large portion (46.3%) of the detected alleles, and 29 unique alleles associated with rice accessions were also discovered. The mean major allele frequency (MAF), genetic diversity (GD) and polymorphic information content (PIC) of the 14 SSR loci were 0.77, 0.36, and 0.34, respectively. A model-based structural analysis revealed the presence of three subpopulations. The genetic relationships revealed by the neighbor-joining tree method were fairly consistent with the structure-based membership assignments for most of the accessions. All 70 accessions showed a clear relationship to each cluster without any admixtures. We observed a relatively low extent of genetic exchange within or among amaranth species from South and Southeast Asia. The genetic diversity results could be used to identify amaranth germplasms and so facilitate their use for crop improvement.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.12 no.6
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• pp.18-35
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• 2009

Distribution of vascular plants in Yangyang-gun were investigated 12 times from Apr. to Sept., 2008. In the result of survey, the flora of this area consist of 412 taxa totally; 89 families, 259 genera, 366 species, 5 subspecies, 37 varieties and 4 forms. There were remarkable plants such as 11 taxa of Korean endemic plants, 58 taxa of specific plants which were designated by the Ministry of Environment, 2 taxa of endangered plants of Ministry of Environment, 7 taxa of rare, endangered plants of Korea Forest Service and 28 taxa of afforestation plants and 296 taxa of useful plants. Furthermore, 36 taxa of naturalized plants (8.74%) were also observed in this area. Vegetation of investigated area was conserved well. However, vegetation has been destroyed rapidly due to construction of commercial complex for human recreation such as condominiums and stores. Therefore, it is need to efficient management of the water quality and vegetation by conservation strategies from huge visitors.

• Korean Journal of Microbiology
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• v.35 no.1
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• pp.61-64
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• 1999

Organophosphorus inseclicide phosphamidon-degrading bacteria were isolated from agricultural soils and identified using Biolog microtiter assay. All Gram-positive degrading bacterial strains belong to genus Bacillus and many Gram-negative bacteria were rare soil species. Among them fast growing strains on phosphamidon-containing minimal medium were sclected and their biodegrading capability wcre measured. YD-17 which was identified as Capnocytophaga gingivalis showed the highest biodegradation rate. It could incrcase the removal of phosphamidon up to 52%. During the biodegradation continuous increase of amount of cell protein was observed, which indicated that phosphamidon was utilized as a carbon source for phosphamidon-degrading bacteria.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.21 no.4
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• pp.25-42
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• 2018

The purpose of this study is to present the basic data for management of ecologically restored stream by surveying and analysing the vascular plants distributed in Chunghyocheon stream, Gyeongju-si. The survey of vascular plants in this site was conducted before and after restoration. The number of vascular plants were summarized as 276 taxa including 68 families, 188 genera, 242 species, 1 subspecies, 29 varieties and 4 forms. The rare plants were 2 taxa including Prunus yedoensis Matsum. and Iris ensata var. spontanea (Makino) Nakai, and the Korean endemic plant was Lespedeza maritima Nakai. The specific plants by floristic region were 13 taxa including 1 taxa of grade V, 3 taxa of grade III, 1 taxa of grade II and 8 taxa of grade I. The naturalized plants were 62 taxa including Persicaria orientalis (L.) Spach, Lepidium virginicum L., Poa compressa L. and so forth. The invasive alien plants were 3 taxa including Ambrosia artemisiifolia L., Aster pilosus Willd. and Lactuca scariola L.. The hydrophytes were 34 taxa including 26 taxa of emergent plant, 1 taxa of floating-leaved plant, 2 taxa of free-floating plant and 5 taxa of submerged plant.

• Tuberculosis and Respiratory Diseases
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• v.46 no.4
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• pp.574-579
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• 1999

Cytomegaloviruses (CMVs) are ubiquitous but highly species-specific agents which commonly infect many animals, including humans. Cytomegalovirus (CMV) pneumonia has been one of the most important opportunistic infections in the immunocompromised host for those who have acquired immunodeficiency syndrome (AIDS) or who have received kidney, bone marrow or other organs. Cytomegalovirus infection has been known to be associated with congenital, infantile and adult nephrotic syndrome. We report a rare case of CMV pneumonia with nephrotic syndrome in a 62-year-old female who recovered fully with ganciclovir.

• Korean Journal of Plant Taxonomy
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• v.38 no.4
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• pp.565-571
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• 2008

In the course of execution for Inventories and Management of Plant Resources of Korea, We found new habitats of 4 plant species which distribution ranges are obscure and/or extremely restricted. A subtropical fern distributed in Jeju-do, Woodwardia japonica (L. f.) Sm., was found in Mt. Gambang-san of Hampyeong-gun, Jeollanam-do. A saprophytic orchid, Galeola septentrionalis Reichb., was found in Mt. Ggachi-bong of Boseong-gun, Jeollanam-do. Peristrophe japonica (Thunb.) Bremek., known in Jeollanam-do, was newly found in Mt. Mudeung-san of Gwangju-si. Viburnum furcatum Blume mainly distributed in Jeju-do and Ulleung-do was found in Mt. Jabyeong-san of Gangneung-si, Gangwon-do.