• Journal of Forest and Environmental Science
• /
• 제25권1호
• /
• pp.57-65
• /
• 2009

Ficus deltoidea Jack is an important and popular medicinal plant species found in the Malaysia. Plants are being collected and used based on morphology and authentication to prevent adulteration is not in practice. In this study, twenty-six accessions of F. deltoidea Jack were collected from Kelantan and Terengganu states of Peninsular Malaysia to examine their genetic similarities and differences using randomly amplified polymorphic DNA (RAPD) technique. Out of 20 arbitrary primers, two primers (D-10 and D-11) were selected which produced reliable DNA polymorphism. D-10 and D-11 primers generated 138 RAPD bands ranging from 250 bp to 3000 bp. Ninety-nine of them were polymorphic loci (72%) and thirty-nine were nonpolymorphic loci (28%). A total of 56 bands with polymorphic loci were amplified with primer D-10 and analyzed by cluster analysis and UPGMA to present a dendrogram depicting the degree of genetic relationship among 26 accessions. Eight RAPD markers were sequenced to determine their identity. RAPD analysis showed the genetic diversity among 26 accessions of F. deltoidea Jack. The RAPD profile and RAPD marker sequences reported in this paper could be used in plant and/or plant material authentication. This study also suggested that RAPD can be a useful technique to study DNA polymorphism in F. deltoidea Jack.

• 한국수산과학회지
• /
• 제36권3호
• /
• pp.317-320
• /
• 2003

Morphologically similar fish species were subjected to the random amplified polymorphic DNA (RAPD) analysis using universal rice primer (URP). The fish species tested were sea basses (Lateolabrax japonicus and L. maculatus), eels (Anguilla japonica, A. bicolor bicolor, A. rostrata, and A. anguilla), and flounders (Limanda yokohamae and L. herzensteinin). Highly reproducible RAPD patterns were observed with several potential species-specific markers. The results indicate that RAPD technique using URP is useful for distinguishing fish psecies in a rapid manner.

• 미생물학회지
• /
• 제37권1호
• /
• pp.56-60
• /
• 2001

탄저균의 분자적 다양성 분석은 다양한 DNA표지의 부족으로 쉬운 일이 아니어서, 본 연구에서는 random amplified polymorphic DNA (RAPD)-PCR을 이용하여 Bacillus 속으로부터 탄저균을 구별할 수 있는 새로운 DNA 표지를 개발하고자 하였다. RAPD-PCR을 이용한 분석은 다양한 Bacillus 종으로부터 탄저균을 동정할 수 있었으며, 아울러 Bacillus 종 사이에서 확실한 유전적인 변이를 확인할 수 있었다. 이러한 분석은 간단, 신속하고, 그리고 정확하게 탄저균을 진단하는데 활용할 수 있다고 본다.

• Journal of Forest and Environmental Science
• /
• 제35권2호
• /
• pp.90-101
• /
• 2019

Biodiversity refers to the total number and variation among species of flora and fauna of an area. Due to tremendous biotic especially anthropogenic pressure these natural resources are being vanishing. In present study genetic diversity among accessions of Thamnocalamus spathiflorus was evaluated. A total of 51 vegetative characters and 42 primers (10-mer) were screened. Out of 42 screened primers, 28 polymorphic primers were selected for further analysis. A total of 263 bands were recorded as polymorphic whereas 48 bands were monomorphic. The resolving power (Rp) of 28 Randomly Amplified Polymorphic DNA (RAPD) primers ranged from 4.6 (OPE08) to 17.6 (OPA11). The polymorphic information content (PIC) value ranged from 0.21 (OPAH09) to 0.44 (OPG02). The result revealed high degree of genetic relatedness (56 to 80%). Cluster analysis revealed two major clusters both for morphology as well as RAPD. Unlike morphological characterization, the accession (D5) from Bahli, Rampur, Shimla (H.P.) was clustered separately from the others in RAPD cluster analysis. Accessions with closed locality grouped together through RAPD marker system however analogy was recorded for morphological traits. The study conducted reflects the utility of RAPD technique for species identification and phylogenetic studies in bamboo for conducting bamboo breeding program.

• Mycobiology
• /
• 제30권1호
• /
• pp.5-12
• /
• 2002

A total of 100 samples of chicken-viscera were collected from different poultry-slaughtering houses in Austria;(20 samples of each of gizzard, heart, intestine, liver and spleen). Intestine and gizzard were heavy contaminated with moulds than other examined visceral organs($4.4{\times}10^5$ and $2.6{\times}10^4$ colonies/1g of the samples, respectively). Fungal contamination was not detected in all samples of heart and spleen. Eighty-five mould isolates were collected from the examined samples, the majority of isolates belonging to Aspergillus glaucus group(20.0%) and Trichoderma(14.1%). These isolates comprised 15 species belonging to 9 genera. Members of Aspergillus glaucus(telomorph: Eurotium) group and Trichoderma were further confirmed their identification using random amplified polymorphic DNA-polymerase chain reaction(RAPD-PCR) technique.

• Asian-Australasian Journal of Animal Sciences
• /
• 제19권4호
• /
• pp.475-481
• /
• 2006

The genetic polymorphism and relationships of Muscovy, Cherry Valley Meat ducks, Partridge ducks and their crossbreds $F_1$ and $F_2$, respectively, were studied using a random amplified polymorphic DNA (RAPD) technique. The results showed that RAPD markers were effective for the analysis of genetic relationships among ducks. Amplification with 20-primers gave 760 reproducible amplified fragments. The percentage of polymorphic marker band was 74.70%, which indicates that the RAPD technique had higher efficiency of polymorphism detection and sensitivity in studying the genetic variations among ducks and showed that the genetic polymorphism was abundant between two species of duck populations. The average index of genetic distance in hybrid $F_2$ was 0.2341 and higher than that of its parents, which indicates that the genetic diversity was improved by crossbreeding with Muscovy.

• 한국가축번식학회지
• /
• 제23권4호
• /
• pp.303-311
• /
• 1999

본 연구는 정자세포로부터 분리된 genome 내 DNA를 PCR 기법으로 증폭시킨 후 random amplified polymorphic DNA(RAPD) 분석을 통해 무지개송어의 품종 내 유전적 특성과 변이성을 해석하고 품종의 특이 유전적 표지를 개발하기 위해서 수행되었다. 20 종류의 primer를 사용하여 RAPD 양상을 검색한 후 다형현상의 출현빈도와 band 수에 기초하여 이들 중 6개의 primer를 선정하여 이용하였다. 그 중에 4개의 primer는 17개의 RAPD marker를 나타내었고, 그중 primer 당 8개인 48개 (28%)의 band 가 다형성을 보여주었다. 6개의 primer 중 4개는 개체들 사이에 다형성을 나타내는 band를 나타내었다. Bandsharing 의 경우 연어와 비교될 만큼 무지개송어는 3개의 특이적인 DNA marker를 가지고 있었다 (2.3. 2.0 및 1.3kb). 같은 무작위 primer를 이용해서 나타난 개별적인 band는 단일 primer 가 무지개송어의 정자핵 DNA의 경우 적어도 3개의 독립적인 genome 내 다형성을 탐지해 낼 수 있다는 것을 제시하고 있다. 이러한 primer에 의해서 나타난 RAPD 다형성은 개체식별을 위한 유전적 표지인자로서 사용될 수 있는 가능성을 제시하였으며, RAPD-PCR은 많은 어종에서 다형현상을 밝혀내는 기술이라 할 수 있다. 본 연구는 RAPD marker가 풍부하고 재현성이 있으며 RAPD 다형성을 지닌 marker를 사용하여 이러한 중요한 양식대상어종에서 미래의 gene mapping과 MAS 를 위한 기초를 제공해 줄 수 있다. RAPD 다형성은 어류의 품종 분화를 위한 유전적 표지로서 유용한 것으로 결론지을 수 있을 것이다.

• 생명과학회지
• /
• 제13권5호
• /
• pp.651-657
• /
• 2003

형태적으로 매우 유사한 적조생물 C. polykrikoides, G. impudicum, G. catenatum을 RAPD 방법을 이용하여 유전적 유연관계를 조사했다. 12개의 primer 중 4종류만 선택되었고 증폭된 밴드수는 59개이며 그 크기는 0.2에서 3.0 kb까지였다. C. polykrikoides, G. impudicum, G. catenatum의 다형화된 밴드수는 16개, 8개, 16개로 각각 나타났다. 반면에, 17개의 밴드만 동일하였다. C. polykrikoides, G. impudicum, G. catenatum의 종 특이적인 밴드수는 26개, 34개, 26개로 각각 보였다. C. polykrikoides와 G. impudicum/G. catenatum의 유전적 유사성은 0.83이며, G. impudicum과 G. catenatum은 0.78로 나타났다. 이러한 결과로 볼 때 형태적으로는 유사하게 보이지만, RAPD 분석에 의하면 C. polykrikoides, G. impudicum, G. catenatum은 현저하게 상이한 적조생물이다. 앞으로 RAPD 기법을 이용하면 이러한 와편모조류의 유전적 변이를 탐색하는데 유용할 것으로 보인다.

• 생명과학회지
• /
• 제16권1호
• /
• pp.88-94
• /
• 2006

본 연구에서 넙치와 조기의 원산지를 판별하기 위한 도구로 RAPD PCR 방법의 가능성을 확인하였다. 넙치는 한국의 주문진 자연산, 통영 양식산, 거제 양식산 그리고 북한 자연산을 실험에 사용하였다. 조기는 한국산과 중국산을 사용하였다. 넙치의 RAPD 패턴에서는 뚜렷하고 일관성이 있는 진단용 띠들을 쉽게 찾을 수 있었다. 조기의 경우에는 유전적인 이질성으로 인하여 각 개체의 RAPD 패턴에서는 일관성이 있는 진단용 띠를 찾기 어려웠지만 각 원산지별로 얻은 RAPD 패턴에서는 가능성이 있는 진단용 띠들을 찾을 수 있었다.

• 한국자원식물학회지
• /
• 제10권3호
• /
• pp.258-264
• /
• 1997

The introduction of molecular biology methodologies to plant improvement programs offers an invaluable opportunity for extensive germplasm characterization. We have applied the developed technique of random amplification of polymorphic DNA(RAPD)to the analysis of evaluating genetic diversity among Korean wild Codonopsis lanceolata. A total of 340 polymorpic hands were gernerated on agarose- and polyacrylamide-gel by 19 primers of abitrary sequence. grouped by cluster analysis using sample matching coefficients of similarity. Among of the samples. the minimum genetic distance value was obtained between sample no. 1(Girisan) and no. 2(Girisan), and the largest value between sample no. 11(Sulaksan) and no. 17(Sulaksan).In separate cluster dendrograms based on agareose - and polyacryamide-gel. some differences were observed; In the case of agarose gel,41 samples could be devided into 7 groups at below about 0.44 level of distance. However they were divided into 6 gourps at below about 0.40 level of distance in the case of polyacrylamide gel. These results showed that polymophic data in agrose were not grouped to wild plant selected from each mountainous district except for wild plants selected from Sulaksan and Chiaksan. We believe that polyacrylamide-RAPD is a superior method for detecting DNA polymorphism compared to agarose-RAPD method.