• Animal Bioscience
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• v.35 no.9
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• pp.1351-1359
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• 2022

Objective: The objective of this study was to analyse the differentially abundant proteins caused by freeze-thawing of ram sperm and explore candidate proteins of interest for their ability to improve ram sperm cryopreservation outcomes in vitro. Methods: Sperm were from three mature Dorper. Fresh and frozen sperm proteins were extracted, and the differentially abundant proteins were analysed by mass spectrometry. Among these proteins, lactoferrin (LTF) was selected to be added before cryopreservation. Next, sperm samples were diluted in Tris extender, with the addition of 0, 10, 100, 500, and 1,000 ㎍/mL of LTF. After thawing, sperm quality was evaluated by motility, plasma membrane integrity, mitochondrial activity and reactive oxygen species (ROS). Results: Cryopreservation significantly altered the abundance of 40 proteins; the abundance of 16 proteins was increased, while that of 24 proteins was decreased. Next, LTF was added to Tris extender applied to ram sperm. The results showed that sperm motility and plasma membrane integrity were significantly improved (p<0.05) by supplementation with 10 ㎍/mL LTF compared to those in the control group. There was no significant difference in mitochondrial activity between the 0 ㎍/mL group and other groups (p>0.05). Supplementation of the cryoprotective extender with 10 ㎍/mL LTF led to decreased ROS levels compared with those in the control and other groups (p<0.05). Conclusion: The LTF is an important protein during cryopreservation, and the addition of 10 ㎍/mL LTF to a cryoprotective extender can significantly improve the function of frozen ram sperm.

• Journal of the Korean Society for Aviation and Aeronautics
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• v.23 no.4
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• pp.117-124
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• 2015

RAM (Reliability, Availability and Maintenance) has been applied to design and analysis tools which affects system's operational sustainability and its life cycle cost as RAM-c(2009 DoD). RAM-c plays also an important role to guarantee the system engineering for mission assurance. Reliability is highly related to the probability of system failure. Availability means mission capability or the condition of ready to mission. Maintenance includes both the repair to recover the system in the event of failure/unexpected breakdown and proactive maintenance to prolong the design service life of the system or machinery. It is the purpose that this paper is to analyze and conclude the objective service life of UAV. The more UAV is operated, the less the level of its reliability becomes. Repairing failures and supplying spare parts on time, system reliability could be improved up until the time over target. Applying statistical Weibull distributions, this paper suggests the analysis of the design service life and economic life of UAV based on RAM-c with operational data.

• 한국전산유체공학회:학술대회논문집
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• 1999.11a
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• pp.77-84
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• 1999

Numerical design optimization techniques are implemented for the improvement of the ram accelerator performance. The design object is to find the minimum ram tube length required to accelerate projectile from initial velocity $V_0$ to target velocity $V_e$. The premixture is composed of $H_2,\;O_2,\;N_2$ and the mole numbers of these species are selected as design variables. The objective function and the constraints are linearized during the optimization process and gradient-based Simplex method and SLP(Sequential Linear Programming) have been employed. With the assumption of two dimensional inviscid flow for internal flow field, the analyses of the nonequilibrium chemical reactions for 8 steps 7 species lave been performed. To determined the tube length, ram tube internal flow field is assumed to be in a quasi-steady state and the flow velocity is divided into several subregions with equal interval. Hence the thrust coefficients and accelerations for corresponding subregions are obtained and integrated for the whole velocity region. With the proposed design optimization techniques, the total ram tube length had been reduced $19\%$ within 7 design iterations. This optimization procedure can be directly applied to the multi-stage, multi-premixture ram accelerator design optimization problems.

• Journal of Korean Institute of Industrial Engineers
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• v.21 no.1
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• pp.17-32
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• 1995

The objective of this study is to develop a general design and performance evaluation model for the system designers in the initial design phase of the integrated automatic manufacturing system based on the RAM(Reliability, Availability and Maintainability) and life cycle cost(LCC). The methodology proposed in this research includes the following two stages. First, a deterministic approach to the solution of optimal work station arrangement for the initial system configuration is considered under the assumption that the system availability is one(no failure and maintenance), and then a stochastic simulation model based on RAM and LCC is developed. Using the results of these two stage simulation, a system performance index(SPI) was developed for the performance evaluation of the proposed system. Also a computer program is developed.

• Journal of computational fluids engineering
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• v.5 no.2
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• pp.55-63
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• 2000

In this paper, the numerical study has been done for the improvement of the superdetonative ram accelerator performance and for the design optimization of the system. The objective function to optimize the premixture composition is the ram tube length, required to accelerate projectile from initial velocity V/sub 0/ to target velocity V/sub e/. The premixture is composed of H₂, O₂, N₂ and the mole numbers of these species are selected as design variables. RSM(Response Surface Methodology) which is widely used for the complex optimization problems is selected as the optimization technique. In particular, to improve the non-linearity of the response and to consider the accuracy and the efficiency of the solution, design space stretching technique has been applied. Separate sub-optimization routine is introduced to determine the stretching position and clustering parameters which construct the optimum regression model. Two step optimization technique has been applied to obtain the optimal system. With the application of stretching technique, we can perform system optimization with a small number of experimental points, and construct precise regression model for highly non-linear domain. The error compared with analysis result is only 0.01% and it is demonstrated that present method can be applied to more practical design optimization problems with many design variables.

• Journal of the Korean Society of Propulsion Engineers
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• v.4 no.2
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• pp.21-30
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• 2000

Numerical design optimization techniques are implemented for the improvement of the ram accelerator performance. The design object is to find the minimum ram tube length required to accelerate projectile from initial velocity $V_o$ to target velocity $V_e$. The premixture is composed of $H_2$, $O_2$, $N_2$ and the mole numbers of these species are selected as design variables. The objective function and the constraints are linearized during the optimization process and gradient-based Simplex method and SLP(Sequential Linear Programming) have been employed. With the assumption of two dimensional inviscid flow for internal flow field, the analyses of the nonequilibrium chemical reactions for 8 steps 7 species have been performed. To determined the tube length, ram tube internal flow field is assumed to be in a quasi-steady state and the flow velocity is divided into several subregions with equal interval. Hence the thrust coefficients and accelerations for corresponding subregions are obtained and integrated for the whole velocity region. With the proposed design optimization techniques, the total ram tube length had been reduced 19% within 7 design iterations. This optimization procedure can be directly applied to the multi-stage, multi-premixture ram accelerator design optimization problems.

• Animal Bioscience
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• v.37 no.5
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• pp.852-861
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• 2024

Objective: The present study aimed to investigate the effect of β-nicotinamide mononucleotide (NMN) supplementation on ram sperm quality during storage at 4℃ in vitro. Methods: Tris-citric acid-glucose solution containing different doses of NMN (0, 30, 60, 90, and 120 µM) was used to dilute semen collected from rams and it was stored at 4℃. Sperm motility, plasma membrane integrity as well as acrosome integrity were evaluated at 0, 24, and 48 h time points after storage at 4℃. In addition, sperm mitochondrial activity, lipid peroxidation (LPO), malondialdehyde (MDA) content, reactive oxygen species (ROS) content, glutathione (GSH) content, superoxide dismutase (SOD) activity, and apoptosis were measured at 48 h time point after storage at 4℃. Results: Results demonstrate that the values obtained for sperm motility, acrosome integrity, and plasma membrane integrity in the NMN treatments were significantly higher than control (p<0.05). The addition of 60 µM NMN significantly improved ram sperm mitochondrial activity and reduced LPO, MDA content, and ROS content compared to control (p<0.05). Interestingly, sperm GSH content and SOD activity for the 60 µM NMN treatment were much higher than those observed for control. NMN treatment also decreased the level of Cleaved-Caspase 3, Cleaved-Caspase 9, and Bax while increasing Bcl-2 level in sperm at 48 h time point after storage at 4℃. Conclusion: Ram sperm quality can be maintained during storage at 4℃ with the addition of NMN at 60 µM to the semen extender. NMN also reduces oxidative stress and apoptosis. Overall, these findings suggest that NMN is efficient in improving the viability of ram sperm during storage at 4℃ in vitro.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.6
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• pp.831-837
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• 2008

The objective of this study was to evaluate the effects of ruminally-protected methionine supplementation (0, 7, or 14 g/head/d) on nutrient intake, digestibility, growth performance, carcass, and meat characteristics of Awassi ram lambs fed finishing diets. Twenty four Awassi ram lambs ($16.8{\pm}1.17kg$ body weight) were randomly assigned to 3 treatment diets (8 lambs/treatment) and housed in individual pens. Lambs were given an adaptation period of 7 days before the intensive feeding period that lasted for 86 days. On day 74 of the trial, a digestibility experiment was performed. At the end of the trial (d 86), all lambs were slaughtered to evaluate carcass characteristics and meat quality. Increasing the level of methionine supplementation did not improve (p>0.05) performance nor feed conversion ratio. Nutrient intake and digestibilities were not influenced (p>0.05) by methionine supplementation. There were no differences in final weight, hot and cold carcass weights, dressing percentages or any of the measured non-carcass components. Tissues and fat depth measurements together with all meat quality attributes measured on longissimus muscle of the loin cut were not affected by methionine supplementation. The only meat quality parameters affected were redness (a*) and the hue angle being higher for the control group (p<0.05). These results suggest that methionine supplementation is not likely to produce any production benefits in nutrient digestibilities, performance or carcass characteristics of ram lambs fed a high performance diet.

• 한국전산유체공학회:학술대회논문집
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• 2000.05a
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• pp.159-165
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• 2000

In this paper, numerical study has been done for the improvement of the superdetonative ram accelerator performance and for the design optimization of the system. The objective function to optimize the premixture composition is the ram tube length required to accelerate projectile from initial velocity $V_o$ to target velocity $V_e$. The premixture is composed of $H_2,\;O_2,\;N_2$ and the mole numbers of these species are selected at design variables. RSM(Response Surface Methodology) which is widely used for the complex optimization problems is selected as the optimization technique. In particular, to improve the non-linearity of the response and to consider the accuracy and efficiency of the solution, design space stretching technique has been applied. Separate sub-optimization routine is introduced to determine the stretching position and clustering parameters which construct the optimum regression model. Two step optimization technique has been applied to obtain the optimal system. With the application of stretching technique, we can perform system optimization with a small number of experimental points, and construct precise regression model for highly non-linear domain. The error to compared with analysis result is only $0.01\%$ and it is demonstrated that present method can be applied more practical design optimization problems with many design variables.

• Journal of the Korean Society of Propulsion Engineers
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• v.14 no.6
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• pp.9-16
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• 2010

The SFRJ(Solid Fuel Ram-Jet) propulsion is attractive for projectiles because of the combination of high propulsive performance and low system complexity more than conventional projectiles. The Objective of this research was to characterize the inlet aerodynamic characteristics (center-body & pitot type) in SFRJ. Diffuser static pressure & combustion chamber pressure was tested and the AoA was changed $0^{\circ}$ and $4^{\circ}$ at Mach number of 3.0 for performance estimate. The performance study of inlet was carried out with the Schlieren system and Supersonic cold-flow system. Under mach 3.0, the center-body showed twice higher total pressure recovering ratio than the pitot type. A Computational fluid dynamic solution is applied internal flow of inlet and the solutions are compared with experimental results.