• 제목/요약/키워드: RAG

검색결과 336건 처리시간 0.031초

RAG-1 and IgM Genes, Markers for Early Development of the Immune System in Olive Flounder, Paralichthys olivaceus

  • Lee, Jang-Wook;Yang, Hyun;Noh, Jae Koo;Kim, Hyun Chul;Park, Choul-Ji;Park, Jong-Won;Hwang, In Joon;Kim, Sung Yeon;Lee, Jeong-Ho
    • 한국발생생물학회지:발생과생식
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    • 제18권2호
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    • pp.99-106
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    • 2014

  • Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival. However, the knowledge of the development of immune system in fish is limited and in demand now. In vertebrates, recombination-activating gene 1 (RAG-1) and immunoglobulin M (IgM) have been considered as very useful markers of the physiological maturity of the immune system. In this study, the expression of the both genes was assessed throughout the early developmental stages of olive flounder larvae (5-55 dph) and used as markers to follow the development of immune system. RAG-1 and IgM mRNA expression was detectable at 5 dph and remained so until 55 dph. These patterns of expression may suggest that the olive flounder start to develop its function around 5 dph. Tissue distribution was found that both genes mRNAs are only expressed in the immune-related organ such as spleen, kidney and gill. The early detection of IgM mRNA led to the investigation of its presence in oocytes. Both RAG-1 and IgM mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred. The biological significance of such a phenomenon remains to be investigated.

  • https://doi.org/10.12717/DR.2014.18.2.099 인용 PDF KSCI

펄스 대전류 Rotary Arc Gap 스위치 개발 (A Development of the Rotary Arc Gap Switch for Pulsed High Current Transfer)

  • 조주현;이홍식;임근희
    • 대한전기학회:학술대회논문집
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    • 대한전기학회 1999년도 하계학술대회 논문집 E
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    • pp.2239-2241
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    • 1999

  • The most important question is how to use which kind of switch in pulsed power generation. There are many kinds of commercial closing switches, which have advantages and disadvantages. The most popular closing switch is the spark gap, but it has a disadvantage in life time, because of erosion of electrodes by arc heating. The Rotary Arc Gap (RAG) switch, especially Walkie-Edgar type RAG switch, was proposed to solve such problems in spark gap. It has a simple and special structure for arc moving caused from self-induced electromagnetic force, because moving arc makes less erosion on the electrodes. In this study we have made an Walkie-Edgar type RAG switch, tested the switching with capacitive energy storage system, and measured rotating arc speed in different peak current.

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낙동강 상류 황지천에 서식하는 쉬리속(genus Coreoleuciscus) 어류 집단의 종 동정 및 잡종 판별 (Species and Hybrid Identification of Genus Coreoleuciscus Species in Hwnag-ji Stream, Nakdong River Basin in Korea)

  • 송하윤;김재훈;서인영;방인철
    • 한국어류학회지
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    • 제29권1호
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    • pp.1-12
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    • 2017

  • 낙동강 상류 지류인 황지천에서 쉬리(Coreoleuciscus splendidus)와 참쉬리(C. aeruginosa)의 종 간 자연잡종 개체를 채집하였다. 쉬리와 참쉬리의 종 간 잡종 개체는 외부형태 비교와 함께 핵 DNA의 RAG1 유전자(1,334 bp)와 미토콘드리아 DNA인 CO1 유전자(1,551 bp)를 이용한 염기서열 분석을 실시하였다. 외부형태 분석결과 잡종 개체는 등지느러미, 꼬리지느러미 및 뒷지느러미 3곳에서 지느러미 반문의 형태가 쉬리와 참쉬리의 중간 형태를 나타내었다. RAG1과 CO1 유전자를 이용한 분자계통 분석결과 황치천에 분포하는 쉬리속 어류는 쉬리, 참쉬리 두 종과 두 종 간의 잡종 개체군으로 구성되어 있음을 확인하였으며, CO1 유전자의 염기서열 분석결과 순종인 정교배체와 잡종인 상반교배체가 잘 구분되었다. 또한 RAG1 유전자 분석결과 13개의 염기서열 변이를 확인하였고, 잡종 개체는 9개의 염기서열에서 double peaks가 확인되었다. 유전학적 분석과 외부형태 변이 분석에 의해 쉬리와 참쉬리 사이에 잡종화가 발생한 것을 확인하였으나 잡종 F2세대와 잡종 F1 세대의 생식적 격리 여부는 확인하지 못하였다.

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