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http://dx.doi.org/10.12717/DR.2014.18.2.099

RAG-1 and IgM Genes, Markers for Early Development of the Immune System in Olive Flounder, Paralichthys olivaceus  

Lee, Jang-Wook (Genetics and Breeding Research Center, NFRDI)
Yang, Hyun (Genetics and Breeding Research Center, NFRDI)
Noh, Jae Koo (Genetics and Breeding Research Center, NFRDI)
Kim, Hyun Chul (Genetics and Breeding Research Center, NFRDI)
Park, Choul-Ji (Genetics and Breeding Research Center, NFRDI)
Park, Jong-Won (Genetics and Breeding Research Center, NFRDI)
Hwang, In Joon (Genetics and Breeding Research Center, NFRDI)
Kim, Sung Yeon (Genetics and Breeding Research Center, NFRDI)
Lee, Jeong-Ho (Genetics and Breeding Research Center, NFRDI)
Publication Information
Development and Reproduction / v.18, no.2, 2014 , pp. 99-106 More about this Journal
Abstract
Fish larvae are immediately exposed to microbes from hatching to maturation of their lymphoid organs, therefore effective innate mechanisms is very important for survival. However, the knowledge of the development of immune system in fish is limited and in demand now. In vertebrates, recombination-activating gene 1 (RAG-1) and immunoglobulin M (IgM) have been considered as very useful markers of the physiological maturity of the immune system. In this study, the expression of the both genes was assessed throughout the early developmental stages of olive flounder larvae (5-55 dph) and used as markers to follow the development of immune system. RAG-1 and IgM mRNA expression was detectable at 5 dph and remained so until 55 dph. These patterns of expression may suggest that the olive flounder start to develop its function around 5 dph. Tissue distribution was found that both genes mRNAs are only expressed in the immune-related organ such as spleen, kidney and gill. The early detection of IgM mRNA led to the investigation of its presence in oocytes. Both RAG-1 and IgM mRNA transcripts were detected in unfertilized oocytes, suggesting that they are maternally transferred. The biological significance of such a phenomenon remains to be investigated.
Keywords
Olive flounder (Paralichthys olivaceus); RAG-1; IgM; Development; Expression; Maternal transfer;
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1 Hirono Ikuo, Nam B-H, Enomoto J, Uchino K, Aoki T (2003) Cloning and characterization of a cDNA encoding Japanese flounder Paralichthys olivaceus IgD. Fish Shellfish Immunol 15:63-70.   DOI   ScienceOn
2 Lee MA, Bengten E, Daggfeldt A, Rytting AS, Pilstrom L (1993) Characterisation of rainbow trout cDNAs encoding a secreted and membrane-bound Ig heavy chain and the genomic intron upstream of the first constant exon. Mol Immunol 30:641-648.   DOI   ScienceOn
3 Huttenhuis HBT, Huisinga MO, Meulen T, Oosterhoud CN, Sanchez NA, Taverne-Thiele AJ, Strobandc HWJ, Rombouta HWM (2005) Rag expression identifies B and T cell lymphopoietic tissues during the development of common carp (Cyprinus carpio). Dev Comp Immunol 29:1033-1047.   DOI   ScienceOn
4 Josefsson S, Tatner MF (1993) Histogenesis of the lymphoid organs in sea bream (Spaurus aurata L.). Fish Shellfish Immunol 3:35-49.   DOI   ScienceOn
5 Kanlis G, Suzuki Y, Tauchi M, Numata T, Shirojo Y, Takashima F (1995) Immunoglobulin in oocytes, fertilized eggs, and yolk sac larvae of red sea bream. Fish Sci 61:787-790.   DOI
6 Magnadottir B (1998) Comparison of immunoglobulin (IgM) from four fishes. Buvisindi Ice Agr Sci 12:47-59.
7 Mao M-G, Lei J-L, Alex P-M, Hong W-S, Wang K-J (2012) Charaterization of RAG1 and IgM (mu chain) marking development of the immune system in redspotted grouper (Epiniphelus akaara). Fish Shellfish Immunol 33:725-735.   DOI   ScienceOn
8 Nagaoka H, Yu W, Nussenzweig MC (2000) Regulation of RAG expression in developing lymphocytes. Curr Opin Immunol 12:187-190.   DOI   ScienceOn
9 Olsen YA, Press CML (1997) Degradation kinetics of immunoglobulin in the egg, alevin and fry of Atlantic salmon, Salmo salar L., the localisation of immuneglobulin in the egg. Fish Shellfish Immunol 7:81-91.   DOI   ScienceOn
10 Padros F, Crespo S (1996) Ontogeny of the lymphoid organs in the turbot Scophthalmus maximus: a light and electron microscope study. Aquaculture 144:1-16.   DOI   ScienceOn
11 Peixoto BR, Mikawa Y, Brenner S (2000) Characterization of the recombinase activating gene-1 and 2 locus in the Japanese pufferfish, Fugu rubripes. Gene 246:275-283.   DOI   ScienceOn
12 Schroder MB, Flano E, Pilstrom L, Jorgensen TO (1998) Localisation of Ig heavy chain mRNA positive cells in Atlantic cod (Gadus morhua L.) tissues; identified by in situ hybridization. Fish Shellfish Immunol 8:565-576.   DOI   ScienceOn
13 Picchietti S, Scapigliati G, Fanelli M, Barbato F, Canese S, Mastrolia L, Mazzini M, Abelli L (2001) Sex-related variations of serum immunoglobulins during reproduction in gilthead sea bream and evidence for a transfer from the female to the eggs. J Fish Biol 59:1503-1511.   DOI
14 Rauta PR, Nayak B, Das S (2012) Immune system and immune responses in fish and their role in comparative immunity study: a model for higher organisms. Immunol Lett 148:23-33.   DOI   ScienceOn
15 Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Misener, S. & S. A. Krawetz (Eds) Bioinformatics Methods and Protocols: Methods in Molecular Biology Humana Press Inc, Totowa (NJ), pp.365-386.
16 Takemura A, Takano K (1997) Transfer of maternallyderived immunoglobulin (IgM) to larvae in tilapia, Oreochromis mossambicus. Fish Shellfish Immunol 7: 355-363.   DOI   ScienceOn
17 Tatner MF (1996) Natural changes in the immune system of fish. In: Iwama, G, Nakanishi T (Eds) The Fish Immune System Organism, Pathogen and Environment Academic Press, San Diego, CA, pp.255-287.
18 Vadstein O (1997) The use of immunostimulation in marine larviculture: possibilities and challenges. Aquaculture 155:401-417.   DOI   ScienceOn
19 Willett CE, Cherry JJ, Steiner LA (1997) Characterization and expression of the recombination activating genes (rag1 and rag2) of zebra fish. Immunogenetics 45:394-404.   DOI
20 Zapata AG, Torroba M, Varas A, Jimenez E (1997) Immunity in fish larvae. Dev Biol Standardizations 90:23-32.
21 Zhang QY, Fan SG, Luo C (2009) Sequence cloning and expression analysis of recombination active gene 1 and 2 in grass carp, Ctenopharyngodon idellus. Acta Hydrobiologica Sin 33:795-803.   DOI
22 Chantanachookhin C, Seikai T, Tanaka M (1991) Comparative study of the ontogeny of the lymphoid organs in three species of marine fish. Aquaculture 99:143-155.   DOI   ScienceOn
23 Dunham M (1999) ika1 and rag1 as markers of the development of the zebra fish immune system. BUG journal 2:49-53.
24 Corripio-Miyar Y, Bird S, Treasurer JW, Secombes CJ (2007) RAG-1 and IgM genes, markers for early development of the immune system in the gadoid haddock, Melanogrammus aeglefinus, L. Fish Shellfish Immunol 23:71-85.   DOI   ScienceOn
25 Covello JM, Bird S, Morrison RN, Bridle AR, Battaglene SC, Secombes CJ, Nowak BF (2013) Isolation of RAG-1 and IgM transcripts from the striped trumpeter (Latris lineata), and their expression as markers for development of the adaptive immune response. Fish Shellfish Immunol 34:778-788.   DOI   ScienceOn
26 Danilova N, Hohman VS, Kim EH, Steiner LA (2000) Immunoglobulin variable region diversity in the zebra fish. Immunogenetics 52:81-91.   DOI
27 Ellis AE (1988) Ontogeny of the immune system in teleost fish. In: Ellis AE (ed) Fish Vaccination Academic Press, London, pp.20-31.
28 Fan SG, Zhang QY, Luo C (2009) Sequence cloning and expression analysis of RAG genes in goldfish. Acta Hydrobiologica Sin 33:603-612.   DOI
29 Hansen JD, Kaattari SL (1995) The recombination activating gene 1 (RAG1) of rainbow trout (Oncorhynchus mykiss): cloning, expression, and phylogenetic analysis. Immunogenetics 42:188-195.
30 Hatten F, Fredriksen A, Hordvik I, Endresen C (2001) Presence of IgM in cutaneous mucus, but not in gut mucus of Atlantic salmon, Salmo salar. Serum IgM is rapidly degraded when added to gut mucus. Fish Shellfish Immunol 11:257-268.   DOI   ScienceOn