• Korean Journal of Food Science and Technology
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• v.34 no.2
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• pp.164-169
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• 2002

Five kinds of red wine from three different grape varieties, such as Gerbong (G), Campbell (C), Moru (M), Gerbong+Moru (70:30, GM), and Gerbong+Campbell(70:30, GC) were prepared in the year of 2000. The total phenolic content and color changes were monitored using hunter colorimeter and spectrophotometry methods during wine processing. The total phenolic content of Gerbong (G), Campbell (C), Moru (M), Gerbong+Moru(70:30, GM), and Gerbong+Campbell(70:30, GC) were 712.6 mg/L, 3472.9 mg/L, 2209.4 mg/L, 2019.4 mg/L, and 1184.5 mg/L, respectively. Between total phenolic content and hunter colorimeter value (L, a, and b) of red wine showed no significant difference, but that of spectrophotometry color value (hue and intensity) were significantly different (p<0.001). The total phenolic content, hue, and intensity values represented significantly different between grape varieties (p<0.01, p<0.001). the hue showed a negative correlation relationship $(r^2=0.8660,\;p<0.0001)$, and that of intensity was a positive $(r^2=0.8304,\;p<0.0001)$ between total phenolic contents. The total phenolic content of red wine could be estimated simply using these equations, $Y_{Total\;phenolic\;content\;(mg/L)}=3319.3-X_{Hue}/2208.36,\;and\;Y_{Total\;phenolic\;content\;(mg/L)}=1230.97-X_{intensity}/98.93$

• Journal of the Korean housing association
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• v.19 no.3
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• pp.11-20
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• 2008

The purpose of this study was to find out tendencies in the planning characteristics focused on the community, single building, shared spaces and interior concept of apartment houses in Daejeon City, and to analyze these characteristics according to apartment unit floor plan based upon the arrangement of room, the type of main entrance, the type of LDK, anbang, kitchen, bathroom and the 3rd space. This study used the content analysis method and analysis was made on drawings and descriptions showing planning characteristics of apartment houses. Above all, 34 cases of apartment houses and 159 unit plans were analyzed. The results of this study were as follows: 1) The most important characteristics of the direction of housing development was the "well-being" concept including greenzone and natural environment. The most mentioned community spaces were children's playgrounds and parks. The emphasized interior concepts were noble, well-being and natural concepts. 2) The most prominent front arrangement type was the 3-bay unit plan, front space arrangement was focused on rooms and, livingroom. The most prominent front space arrangement types were R/L/R, R/L/R/R and there is a new tendency towards KD/L/R/R. 3) The most prominent LDK floor plans were of the LDK type. The type of "Anbang" arrangement consisted mainly of room-bathroom-dressing room. The type of public bathroom arrangement consisted mainly of bathtub-toilet-washbasin and the "Anbang" bathroom arrangement was shower-booth-toilet-washbasin.

• YAKHAK HOEJI
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• v.54 no.5
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• pp.398-402
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• 2010

[ ${\alpha}$ ]Glucosidase inhibitor is a target in the treatment of type II diabetes through the mainly inhibition of glucose levels after meals. In this study, we purified steppogenin and oxyresveratrol from the stem of Morus alba L. and examined their inhibitory activity against yeast ${\alpha}$-glucosidase. Steppogenin and oxyresveratrol were inhibited yeast ${\alpha}$-glucosidase in a dose dependent manner. The $IC_{50}$ activities (50% inhibition) were 34.4 and 9.3 ${\mu}M$, respectively. The kinetic inhibition of steppogenin showed noncompetitive inhibition ($K_m:1.1{\times}10^{-3}M$; $K_i:1{\times}10^{-5}M$), meanwhile oxyresveratrol showed competitive inhibition ($K_m:4.3{\times}10^{-3}M$; $K_i:3.4{\times}10^{-6}M$) against yeast ${\alpha}$-glucosidase. These results indicate that steppogenin and oxyresveratrol are noncompetitive and competitive inhibitors, respectively, against yeast ${\alpha}$-glucosidase.

• Journal of Microbiology and Biotechnology
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• v.34 no.3
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• pp.700-709
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• 2024

Polyhydroxybutyrate (PHB) production from lignocellulosic biomass is economically beneficial. Because lignocellulosic biomass is a mixture rich in glucose and xylose, Escherichia coli, which prefers glucose, needs to overcome glucose repression for efficient biosugar use. To avoid glucose repression, here, we overexpressed a xylose regulator (xylR) in an E. coli strain expressing bktB, phaB, and phaC from Cupriavidus necator and evaluated the effect of xylR on PHB production. XylR overexpression increased xylose consumption from 0% to 46.53% and produced 4.45-fold more PHB than the control strain without xylR in a 1% sugar mixture of glucose and xylose (1:1). When the xylR-overexpressed strain was applied to sugars from lignocellulosic biomass, cell growth and PHB production of the strain showed a 4.7-fold increase from the control strain, yielding 2.58 ± 0.02 g/l PHB and 4.43 ± 0.28 g/l dry cell weight in a 1% hydrolysate mixture. XylR overexpression increased the expression of xylose operon genes by up to 1.7-fold. Moreover, the effect of xylR was substantially different in various E. coli strains. Overall, the results showed the effect of xylR overexpression on PHB production in a non-native PHB producer and the possible application of xylR for xylose utilization in E. coli.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.34 no.3
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• pp.190-193
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• 2001

To investigate the effects of the dissolved oxygen concentration (DO) on Scope for growth (SFG) of the manila clam, Ruditapes philippinarum, we measured $LC_{50}$, filtration, respiration, ammonia excretion, and assimilation rates under $23\pm0.5^{\circ}C$ as a function of DO. The $LC_{50}$ of DO for R. philippinarum, was 2.4 mgDO $L^{-1}$. With decreasing DO, filtration and respiration rates of R. philippinarum decreased, while ammonia excretion rate increased, The assimilation rate was $68.2\%$ at 6.5 mgDO $L^{-1}$, decreased to $29.8\~39.3\%$ at 3.5 mgDO $L^{-1}$. R. philippinarum had positive SFG's at the $DO{\geq}2.5mgDO\;L^{-1}$.

• Journal of Pharmaceutical Investigation
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• v.34 no.5
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• pp.385-391
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• 2004

The purpose of the present study was to formulate the aqueous solution of $20-O-{\beta}-D-glucopyranosyl-20(S)-protopanaxadiol\;(IH-901)$, an intestinal bacterial metabolic derivative from Ginseng protopanaxadiol saponin. For this purpose, the effects of various solubilization agents such as cosolvents [ethanol, propylene glycol (PG), polyethylene glycol 300 (PEG 300), polyethylene glycol 400 (PEG 400), glycerin], surfactants $(Tween\;80,\;Cremophor^{\circledR}\;RH40,\;Cremophor^{\circledR}\;EL,\;Poloxamer\;407,\;Poloxamer\;188)$ and a complexation agent $[hydroxypropyl-{\beta}-cyclodextrin\;(HPBCD)]$, on the solubility of IH-90l in aqueous solution were evaluated. The solubility of IH-901 in water was under $1\;{\mu}g/ml\;at\;20^{\circ}C$. Cosolvents such as ethanol, PG, PEG 300, PEG 400 and glycerin did not enhance the solubility of IH-901 at the 0 - 40% concentration range. The solubility of IH-901 was significantly elevated by the addition of cosolvents over the 80% concentration range. On the other hand, tween 80, $Cremophor^{\circledR}\;EL,\;Cremophor^{\circledR}\;RH40$ and HPBCD showed enhanced effects on the solubility of IH-901. The enhanced effects of Poloxamer 407 or Poloxamer 188 on the IH-901 solubility were less pronounced compared with $Cremophor^{\circledR}\;EL\;or\;Cremophor^{\circledR}\;RH40$. As a results, $Cremophor^{\circledR}$ aqueous solution was selected as an optimum solvent system. The aqueous solutions containing 10% $Cremophor^{\circledR}\;EL$ and 7% $Cremophor^{\circledR}\;RH40$ were formulated as dosing solutions containing 5.0 mg/ml of IH-901 for its intravenous and oral administration, respectively. The formular showed physical stability after stored for 7 days at $4^{\circ}C$.

• Journal of the Korean Chemical Society
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• v.60 no.4
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• pp.225-234
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• 2016

The activity of 34 sulfonamide derivatives has been estimated by means of multiple linear regression (MLR), artificial neural network (ANN), simulated annealing (SA) and genetic algorithm (GA) techniques. These models were also utilized to select the most efficient subsets of descriptors in a cross-validation procedure for non-linear -log (IC50) prediction. The results obtained using GA-ANN were compared with MLR-MLR, MLR-ANN, SA-ANN and GA-ANN approaches. A high predictive ability was observed for the MLR-MLR, MLR-ANN, SA-ANN and MLR-GA models, with root mean sum square errors (RMSE) of 0.3958, 0.1006, 0.0359, 0.0326 and 0.0282 in gas phase and 0.2871, 0.0475, 0.0268, 0.0376 and 0.0097 in solvent, respectively (N=34). The results obtained using the GA-ANN method indicated that the activity of derivatives of sulfonamides depends on different parameters including DP03, BID, AAC, RDF035v, JGI9, TIE, R7e+, BELM6 descriptors in gas phase and Mor 32u, ESpm03d, RDF070v, ATS8m, MATS2e and R4p, L1u and R3m in solvent. In conclusion, the comparison of the quality of the ANN with different MLR models showed that ANN has a better predictive ability.

• Journal of Forest and Environmental Science
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• v.34 no.6
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• pp.419-428
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• 2018

Spatial point-patterns analyses were conducted to provide insight into the ecological process behind competition and mortality in two lodgepole pine (Pinus contorta Dougl. ex Loud. var. latifolia Engelm.) stands, one in the Lower Foothills, and the other in the Upper Foothills natural subregions in the boreal forest of Alberta, Canada. Spatial statistical tests were applied to live and dead trees and included Clark-Evans nearest neighbor statistic (R), nearest neighbor distribution function (G(r)), and a variant of Ripley's K function (L(r)). In both lodgepole pine plots, the results indicated that there was significant regularity in the spatial point-pattern of the surviving trees which indicates that competition has been a key driver of mortality and forest dynamics in these plots. Dead trees generally showed a clumping pattern in higher density patches. There were also significant bivariate relationships between live and dead trees, but the relationships differed by natural subregion. In the Lower Foothills plot there was significant attraction between live and dead tees which suggests mainly one-sided competition for light. In contrast, in the Upper Foothills plot, there was significant repulsion between live and dead trees which suggests two-sided competition for soil nutrients and soil moisture.

• Laboraroty Animal Research
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• v.34 no.4
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• pp.211-215
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• 2018

The following experiments were designed to examine the effect of serum of spayed dogs on superovulation response in mice and rats. In Experiment 1, female mice at diestrus (n=30) were divided into three equal groups and superovulated with either administration of 5 IU pregnant mare serum gonadotropin (PMSG) or recombinant follicle stimulating hormone (rFSH) (reducing dose from 2.5 to 0.5 IU) and 5 IU human chorionic gonadotropin (hCG) administered 48h later. Serum of spayed dogs was administered intraperitoneally at a reduced dose from 0.1 to 0.025 mL in a 48 h period. In Experiment 2, female rats (n=30) at diestrus stage were divided into three equal groups. Superovulation was induced using either 30 IU PMSG, or a dose reduced from 5 to 1 IU rFSH and 25 IU hCG administered 48h later. Serum of spayed dogs was administered in a reduced dose from 0.6 to 0.1 mL in a 48 hour period. Female mice and rats were mated 24 h following hCG administration. On day 14 after mating, animals were euthanized and ovarian sections were fixed for histopathological evaluation and corpus luteum (CL) counting. No significant difference observed in mean (${\pm}SEM$) number of CLs between the PMSG group and the mice that received serum of spayed dog ($10.4{\pm}1.3$ vs $9.2{\pm}1.0$). Mean (${\pm}SEM$) number of CLs tended to be lower in rats that received serum of spayed dog than those of rats which received either PMSG or rFSH ($15.1{\pm}1.9$ vs $23.6{\pm}3.1$ and $23.1{\pm}2.9$, P=0.06, respectively). In conclusion, serum of spayed dogs is able to induce a superovulatory response in mice and rats.

• Journal of the Korean Institute of Gas
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• v.2 no.2
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• pp.34-39
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• 1998

Demand of the clean and convenient natural gas has continuously increased with recognizing of the environment problem since liquefied natural gas was introduced in Korea. Clean fuel natural gas was supplied to each city through high tensile strength pipeline connected by welding. Grades of pipeline were divided into the high and middle pressure according to supply pressure. Pipeline was welded mainly SMA welding process due to its easy handling, the other welding process was adopted according to the constructing condition. We were examined on the microstructure variation and mechanical properties of weld metal for high pressure pipeline, API 5L X-42.