• 제목/요약/키워드: R-SER

검색결과 102건 처리시간 0.021초

A New Protein Factor in the Product Formation of Non-Reducing Fungal Polyketide Synthase with a C-Terminus Reductive Domain

  • Balakrishnan, Bijinu;Chandran, Ramya;Park, Si-Hyung;Kwon, Hyung-Jin
    • Journal of Microbiology and Biotechnology
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    • 제25권10호
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    • pp.1648-1652
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    • 2015
  • Azaphilone polyketides are synthesized by iterative non-reducing fungal polyketide synthases (NR-fPKSs) with a C-terminus reductive domain (-R). Several azaphilone biosynthetic gene clusters contain a putative serine hydrolase gene; the Monascus azaphilone pigment (MAzP) gene cluster harbors mppD. The MAzP productivity was significantly reduced by a knockout of mppD, and the MAzP NR-fPKS-R gene (MpPKS5) generated its product in yeast only when co-expressed with mppD. Site-directed mutations of mppD for conserved Ser/Asp/His residues abolished the product formation from the MpPKS5/mppD co-expression. MppD and its homologs are thus proposed as a new protein factor involved in the product formation of NR-fPKS-R.

유산균 발효유의 이용과 건강증진 (Utilization of Fermented Milk and It's Health Promotion)

  • 이정열;허철성;백영진
    • Journal of Dairy Science and Biotechnology
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    • 제17권1호
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    • pp.58-71
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    • 1999
  • This study was designed to investigate the health promotion effect of fermented milk and historical story of Korean dairy products from the ancient period to present. Although the origin of fermented milk is Europe, the recede of fermented milk was founded in far-east and middle east areas at BC 4C. After the spread of fermented milk to Korea and Japan. The consumption of fermented milk in Korea was dramatically increased to 14.2 kg per person in 1997. Health promotion effect of fermented milk can be devided to 5 major effected improvements of intestinal microflora, anticancer, cholesterol assimilation anti-pathogenic activity. Fermented milk reduced the level of ${\beta}$-glucornidase and nitroreductase to 50% and it provides anticancer activity by cell wall an polysaccharides. Fermented milk has cholesterol assimilation activity ca. 54${\sim}$40% (B. longum, Str. thermophillus). Anti-pathogenic activity of fermented milk was significant. It appeared that Sal. ser. typhimurium was more susceptible than 5. coli 0157 at low pH fermented milk. Viable cells of E. coli 0157 were not dramatically decreased in most of fermented milks tested, but in general, Sal. ser. typhimurium was drastically decreased in most of the fermented milks.

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ser-M Framework을 활용한 공급망관리(SCM) 메커니즘에 관한 연구 - 글로벌 철강사 사례를 중심으로 - (A Study on Supply Chain Management Mechanism Using ser-M Framework - Focused on the case of Global Steel Manufacturer -)

  • 홍성식;엄재근
    • 국제지역연구
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    • 제22권4호
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    • pp.77-98
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    • 2018
  • 본 논문의 목적은 제조업, 특히 철강기업의 공급망 경쟁력 확보를 위한 최적의 메커니즘 사례를 연구함으로서 그 시사점을 찾고자 하는 것이다. 이를 위해 본 논문에서는 국내 대표 글로벌 철강그룹의 계열사를 대상으로 그 사례를 살펴보았다. 지금까지의 기업 전략연구는 주로 대형 그룹사의 모기업을 중심으로 이루어져 왔으나, 이번에는 대그룹 계열사에 대한 전략적 한계점과 특성을 고려하여 ser-M기반에서 공급망관리 메커니즘을 다루도록 하며, 이를 통해 글로벌 철강업체 및 대기업 계열사에 적절한 메커니즘을 탐색해보고자 한다. 연구결과, 대기업 그룹의 계열사는 그룹 모기업의 필요에 의해 건립되는 경우가 많아 모기업(S) 중심의 메커니즘이 작동되기 쉽다. 따라서 계열사 입장에서 통제 가능한 내부 자원(R)을 최대한 활용하는 메커니즘이 적합한 것으로 나타났다. 본 논문의 ser-M기반 공급망 메커니즘 연구는 글로벌 철강 제조업 공급망 사례를 중심으로 메커니즘을 살펴보았다는데 의미가 있으며, 또한 국내 대표그룹 계열사에 대한 사례연구란 점에서 그 의의를 찾을 수 있다.

Vanillic Acid Stimulates Anagen Signaling via the PI3K/Akt/β-Catenin Pathway in Dermal Papilla Cells

  • Kang, Jung-Il;Choi, Youn Kyung;Koh, Young-Sang;Hyun, Jin-Won;Kang, Ji-Hoon;Lee, Kwang Sik;Lee, Chun Mong;Yoo, Eun-Sook;Kang, Hee-Kyoung
    • Biomolecules & Therapeutics
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    • 제28권4호
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    • pp.354-360
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    • 2020
  • The hair cycle (anagen, catagen, and telogen) is regulated by the interaction between mesenchymal cells and epithelial cells in the hair follicles. The proliferation of dermal papilla cells (DPCs), mesenchymal-derived fibroblasts, has emerged as a target for the regulation of the hair cycle. Here, we show that vanillic acid, a phenolic acid from wheat bran, promotes the proliferation of DPCs via a PI3K/Akt/Wnt/β-catenin dependent mechanism. Vanillic acid promoted the proliferation of DPCs, accompanied by increased levels of cell-cycle proteins cyclin D1, CDK6, and Cdc2 p34. Vanillic acid also increased the levels of phospho(ser473)-Akt, phospho(ser780)-pRB, and phospho(thr37/46)-4EBP1 in a time-dependent manner. Wortmannin, an inhibitor of the PI3K/Akt pathway, attenuated the vanillic acid-mediated proliferation of DPCs. Vanillic acid-induced progression of the cell-cycle was also suppressed by wortmannin. Moreover, vanillic acid increased the levels of Wnt/β-catenin proteins, such as phospho(ser9)-glycogen synthase kinase-3β, phospho(ser552)-β-catenin, and phospho(ser675)-β-catenin. We found that vanillic acid increased the levels of cyclin D1 and Cox-2, which are target genes of β-catenin, and these changes were inhibited by wortmannin. To investigate whether vanillic acid affects the downregulation of β-catenin by dihydrotestosterone (DHT), implicated in the development of androgenetic alopecia, DPCs were stimulated with DHT in the presence and absence of vanillic acid for 24 h. Western blotting and confocal microscopy analyses showed that the decreased level of β-catenin after the incubation with DHT was reversed by vanillic acid. These results suggest that vanillic acid could stimulate anagen and alleviate hair loss by activating the PI3K/Akt and Wnt/β-catenin pathways in DPCs.

MicroRNA-122 Promotes Proliferation, Invasion and Migration of Renal Cell Carcinoma Cells Through the PI3K/Akt Signaling Pathway

  • Lian, Ji-Hu;Wang, Wei-Hua;Wang, Jia-Qiang;Zhang, Yu-Hong;Li, Yi
    • Asian Pacific Journal of Cancer Prevention
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    • 제14권9호
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    • pp.5017-5021
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    • 2013
  • Objective: MicroRNAs (miRNAs) are a small class of non-coding, single-stranded RNAs with a critical role in genesis and maintenance of renal cancer mainly through binding to 3'-untranslated regions (3'UTR) of target mRNAs, which causes a block of translation and/or mRNA degradation. The aim of the present study was to investigate the potential effects of miR-122 in human renal cell carcinomas. Methods: The expression level of miR-122 was quantified by qRT-PCR. MTT, colony formation, invasion and migration assays were used to explore the potential functions of miR-122 in human renal cell carcinoma cells. Results: Cellular growth, invasion and migration in two A498 and 786-O cells were significantly increased after miR-122 transfection. Further experiments demonstrated that overexpression of miR-122 resulted in the increase of phospho-Akt (Ser473) and phospho-mTOR (Ser2448), then activation of mTOR targets, p70S6K and 4E-BP1. Conclusions: The up-regulation of miR-122 may play an important role in the progress of renal cancer through activating PI3K/Akt signal pathway and could be a potential molecular target for anti-cancer therapeutics.

Purification and Characterization of the Anabolic Acetolactate Synthase III from Serratia marcescens ATCC 25419

  • Joo, Han-Seung;Kim, Soung-Soo
    • BMB Reports
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    • 제34권3호
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    • pp.244-249
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    • 2001
  • The anabolic acetolactate synthase III was purified to homogeneity from Serratia marcescens using DEAE-Sepharose, Phenyl-Sepharose, and hydroxylapatite column chromatography The native molecular weight of the enzyme was approximately 165 kDa. The enzyme is composed of two large and two small subunits with molecular weights of 64 and 15 kDa, respectively. The N-terminal sequence of the large and small subunit of the enzyme was Ser-Ala-Thr-Pro-Gln-Pro-Ser-Thr-Arg-Phe-Thr-Cys-Ala-Gln-Leu-Ile-Ala-His-Leu and Met-Leu-Gln-Pro-Gln-Asp-Lys-Pro-Gln-Val-Ile-Leu-Glu-Leu-Ala-Val-Arg-Asn-His-Pro-Gly-Val-Met-Ser-His-Val, respectively. The optimum pH and pI value were 7.5 and 5.5, respectively The $IC_{50}$ values were $20\;{\mu}M$ and $14\;{\mu}M$ for valine and herbicide SU7, respectively. The substrate specificity ratio, R value, was determined to be approximately 40, which suggests that this enzyme prefers the formation of $\alpha$-aceto-$\alpha$-hydroxybutyrate leading to the synthesis of isoleucine.

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역교잡반응법을 이용한 아이소니아지드 및 리팜피신 신속감수성검사 (Rapid Drug Susceptibility Testing for Isoniazid and Rifampicin by Reverse Hybridization Assay)

  • 박영길;유희경;류성원;배길한
    • Tuberculosis and Respiratory Diseases
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    • 제55권5호
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    • pp.440-448
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    • 2003
  • 연구배경 : 다제내성균의 신속한 확인을 가능케 해주는 감수성검사법은 신속한 처방결정을 통해 환자의 치료 성공률을 향상 시킴과 동시에 다제내성균의 전파를 조기에 차단할 수 있게 되어, 국가 결핵관리 사업의 효율성을 증대시킬 수 있다. 방 법 : 아이나 또는 리팜피신 내성에 관련된 유전자 rpoB, katG, inhA, ahpC 등의 돌연변이를 검출할 수 있는 probe를 합성하였고, 총 502개의 내성균을 대상으로 중함효소연쇄반응 역교잡반응법으로 내성균 검출을 시도하였다. 결 과 : 264개의 리팜피신 내성균 중에서 Ser531Leu돌연변이가 46%를 차지하였고, codon 526에서는 32% 의 균주에서 돌연변이를 보였으며, codon 516에서는 10%의 균주가 돌연변이를 나타냈다. 469개의 아이나 내성균 중에서는 64%가 katG 유전자의 Ser315Thr 돌연변이를 나타내었고, 19%의 균은 inhA 유전자 promoter 돌연변이를 가지고 있었으며, ahpC유전자 돌연변이는 3%에 불과하였다. 역교잡반응법에 의한 검출율은 아이나 내성균 중 80%이상이었으며, 리팜피신 내성균에서도 92%이상을 검출할 수 있었다. 결 론 : 역교잡반응법에 의해 리팜피신 뿐만 아니라 아이나에 대한 감수성검사를 신속하게 수행할 수 있음을 확인하였고, 이 검사법은 특히 재발 또는 다제내성이 의심되는 환자의 처방 결정에 매우 유용한 수단이 될 수 있다.

Repairing Damaged Hair Using Pentapeptides of Various Amino Acid Sequences with Crosslinking Reaction

  • Choi, Wonkyung;Son, Seongkil;Song, Sang-Hun;Kang, Nae Gyu;Park, Sun-gyoo
    • Korea Journal of Cosmetic Science
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    • 제2권1호
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    • pp.11-19
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    • 2020
  • The aim of this study is to investigate the effect of various pentapeptides on hair repair depending on the characteristics of comprising amino acids using crosslinking agents in hair. Total ten peptides were synthesized with two kinds of amino acids respectively, of which were previously categorized according to R group of the amino acids contributing to the characteristic of each peptide: STTSS (Ser-Thr-Thr-Ser-Ser), LIILL (Leu-Ile-Ile-Leu-Leu), CMMCC (Cys-Met-Met-Cys-Cys), DEEDD (Asp-Glu-Glu-Asp-Asp), RKKRR (Arg-Lys-Lys-Arg-Arg), TAMRA-STTSS, TAMRA-LIILL, TAMRA-CMMCC, TAMRA-DEEDD, and TAMRA-RKKRR. Pentapeptide alone, or pentapeptides with crosslinking agents such as polymeric carbodiimide (PCI) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were treated to chemically damaged hair. Hair diameter and break strength (N = 40/case) were measured to calculate tensile strength of hair for computing hair repair ratio, and fluorescence yields (N = 20/case) were collected for hair treated with TAMRA-peptides. The tensile strength of hair treated with pentapeptides alone, or pentapeptides with cross-linking agents is consistent with the fluorescence yield from the microscope images of the cross-sectioned hair in vision and in numerical values. Pentapeptides consisting of hydrophobic amino acids (LIILL), amino acids with sulfur (CMMCC), and basic amino acids (RKKRR) increased the tensile strength in perm-damaged hair. Pentapeptides with no extra carboxyl/amine groups in R group of amino acids resulted in no significant differences in hair strength and fluorescence yield among hairs treated with alone and with crosslinkers. Pentapeptides with extra carboxyl groups or amine groups enabled further strengthening of hair due to increased bonds within the hair after carbodiimide coupling reaction. The hair repairs of pentapeptides with various amino acid sequences were studied using crosslinking. Depending on the physical characteristics of comprising amino acids, the restoration of damaged hair was observed with tensile strength of hair and fluorescence signals upon cross-sectioned hair in parallel to possibly understand the binding tendency of each pentapeptide within the hair.

Phosphorylation of REPS1 at Ser709 by RSK attenuates the recycling of transferrin receptor

  • Kim, Seong Heon;Cho, Jin-hwa;Park, Bi-Oh;Park, Byoung Chul;Kim, Jeong-Hoon;Park, Sung Goo;Kim, Sunhong
    • BMB Reports
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    • 제54권5호
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    • pp.272-277
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    • 2021
  • RalBP1 associated EPS domain containing 1 (REPS1) is conserved from Drosophila to humans and implicated in the endocytic system. However, an exact role of REPS1 remains largely unknown. Here, we demonstrated that mitogen activated protein kinase kinase (MEK)-p90 ribosomal S6 Kinase (RSK) signaling pathway directly phosphorylated REPS1 at Ser709 upon stimulation by epidermal growth factor (EGF) and amino acid. While REPS2 is known to be involved in the endocytosis of EGF receptor (EGFR), REPS1 knockout (KO) cells did not show any defect in the endocytosis of EGFR. However, in the REPS1 KO cells and the KO cells reconstituted with a non-phosphorylatable REPS1 (REPS1 S709A), the recycling of transferrin receptor (TfR) was attenuated compared to the cells reconstituted with wild type REPS1. Collectively, we suggested that the phosphorylation of REPS1 at S709 by RSK may have a role of the trafficking of TfR.

Physical nature of the eclipsing δ Scuti star AO Serpentis

  • Park, Jang-Ho;Lee, Jae Woo;Hong, Kyeongsoo;Koo, Jae-Rim;Kim, Chun-Hwey
    • 천문학회보
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    • 제44권1호
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    • pp.86-86
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    • 2019
  • We present BV photometric observations and high-resolution spectra of AO Ser, which were obtained at the Mt. Lemmon Optical Astronomy Observatory (LOAO) and the Bohyunsan Optical Astronomy Observatory (BOAO), respectively, in 2017. The radial velocities (RVs) for both components were measured, and the effective temperature of the primary star was found to be $T_{eff,1}=8,820{\pm}62K$ by a comparison of the observed spectra and the Kurucz models. A unique set of fundamental parameters of AO Ser were derived for the first time by a simultaneous analysis of the light and RV curves. The results indicate that our program target is a semi-detached eclipsing system with values of $M_1=2.06{\pm}0.11M_{\odot}$ and $M_2=0.41{\pm}0.03M_{\odot}$, $R_1=1.54{\pm}0.03R_{\odot}$ and $R_2=1.30{\pm}0.02R_{\odot}$, and $L_1=12.9{\pm}0.2L_{\odot}$ and $L_2=0.9{\pm}0.3L_{\odot}$. We applied multiple frequency analyses to the eclipse-subtracted light residuals. As a result, two frequencies of $f_1=21.85151days^{-1}$ and $f_2=23.48405days^{-1}$ were detected and their pulsation constants were calculated to $Q_1=0.0344days$ and $Q_2=0.0320days$. The pulsational characteristics and the position in the HR diagram demonstrate that the primary star is a ${\delta}$ Sct pulsator.

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